中国四川鸡传染性支气管炎病毒的分离与鉴定
Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China.
摘要(中文译文)
从疑似传染性支气管炎感染的鸡的肾脏和肺中分离到一种非血凝性病毒。以无特定病原体鸡胚作为培养系统。利用鸡胚气管器官培养的纤毛活性作为指示系统，显示其中一株分离株（SAIB3）的理化特性与传染性支气管炎病毒（IBV）M41株（标准株）相似；而该分离株的电镜观察显示为冠状病毒颗粒。在气管器官培养中进行病毒中和试验，基于交互中和滴度和欧几里得距离比较了5株IBV分离株与6株已知IBV株的血清型。交叉中和模式表明，一株分离株为T株IBV血清型，另一株为M41 IBV血清型，而其他分离株与IBV的M41株和T株具有部分血清型关系。
Abstract
A nonhemagglutinating virus was isolated from kidneys and lungs of chickens suspected of having infectious bronchitis infection. Specific-pathogen-free embryonated chicken eggs were used as the cultural system. With the use of the ciliary activity of chicken embryo tracheal organ cultures as indicator system, the physicochemical properties of one of the isolated strains (SAIB3) were shown to be similar to infectious bronchitis virus (IBV) strain M41 (standard strain); whereas electron microscopy of the isolate showed coronavirus particles. Virus-neutralization tests were performed in tracheal organ cultures to compare the serotypes of five IBV isolates and six known IBV strains on the basis of reciprocal neutralization titers and euclidean distance. The cross-neutralization pattern indicated that one isolate was of the T-strain IBV serotype, another of the M41 IBV serotype, while others had partial serotype relationship to M41 and T-strains of IBV.
如何引用
AMA
H N Wang, Q Z Wu, Y Huang, P Liu. Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China.. Avian diseases.
APA
H N Wang, Q Z Wu, Y Huang, P Liu Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China.. Avian diseases.
GB/T 7714
H N Wang, Q Z Wu, Y Huang, P Liu. Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China.[J]. Avian diseases
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