从濒危草原松鸡中分离出的网状内皮组织增殖病病毒的致病性及传播

摘要(中文译文)

采用日本鹌鹑实验模型研究了网状内皮组织增殖病病毒（REV）野外分离株的致病性和传播性。同时在鸡和火鸡接种后评估了其致瘤性。原始REV（命名为APC-566）分离自阿特沃特草原鸡（Tympanuchus cupido attwateri），该物种是美国南部一种濒危野生禽类。REV分离株的传播性通过与实验感染鹌鹑接触的幼龄未感染日本鹌鹑进行研究。通过病毒分离和间接免疫荧光未检测到垂直传播。在少数接触鹌鹑中检测到血清阳转，提示存在水平传播。APC-566分离株在胚胎期感染的鹌鹑中于6周龄开始诱导肿瘤。日本鹌鹑中检测到的大多数肿瘤为淋巴肉瘤，其中81%的肿瘤通过免疫过氧化物酶染色含有CD3+细胞。REV APC-566对1日龄感染的鸡和火鸡也具有致瘤性，鸡在感染后最早58天出现肿瘤，火鸡在13周龄时出现肿瘤。本研究的部分目的是试图了解从濒危禽类中分离的REV的致病性和传播潜力。

Abstract

The pathogenicity and transmission of a field isolate of reticuloendotheliosis virus (REV) was studied using an experimental model in Japanese quail. Oncogenicity was also evaluated after inoculations in chickens and turkeys. The original REV (designated APC-566) was isolated from Attwater's prairie chickens (Tympanuchus cupido attwateri), an endangered wild avian species of the southern United States. The transmissibility of the REV isolate was studied in young naive Japanese quail in contact with experimentally infected quail. Vertical transmission was not detected by virus isolation and indirect immunofluorescence. Seroconversion was detected in few contact quails, suggesting horizontal transmission. The APC-566 isolate induced tumors beginning at 6 wk of age in quails infected as embryos. Most of the tumors detected in Japanese quail were lymphosarcomas, and 81% of these neoplasias contained CD3+ cells by immunoperoxidase. REV APC-566 was also oncogenic in chickens and turkeys infected at 1 day of age, with tumors appearing as early as 58 days after infection in chickens and at 13 wk of age in turkeys. This study was conducted in part as an attempt to understand the potential for pathogenicity and transmission of REV isolated from endangered avian species.

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