Synthetic and Systems Biotechnology合成与系统生物技术
Synthetic and Systems Biotechnology(英文缩写 SYN SYST BIOTECHNO),ISSN 2405-805X,eISSN 2405-805X,中文译名:合成与系统生物技术 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.692 | Q2 |
| 2022 | 4.800 | Q1 |
| 2023 | 4.400 | Q1 |
| 2024 | 4.400 | Q1 |
| 2025 | 4.800 | Q2 |
Synthetic and Systems Biotechnology 最新收录文献
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1. Hijacking host RNA polymerase for processive in situ mutagenesis.
PMID:日期:2027-04-01Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host compatibility, operational complexity, and uneven distribution of mutations across these windows. In this study, we developed a novel in vivo Targeted Assisted Mutagenesis via Endogenous RNAP tool (TAMER) by functionally fusing the endogenous ω subunit of RNAP with a deaminase and dCas9. This tool achieved a mutagenesis efficiency of 1.28 × 10 substitutions per base (s.p.b.), which represents a 4.2 × 10-fold increase over the natural mutation rate. Additionally, this method enables uniform mutation distribution, an even mutation rate, a broad mutagenesis window, low off-target effects, and cross-host portability. Overall, TAMER offers a simple, efficient, and broadly applicable strategy for in vivo targeted long-range mutagenesis.
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2. {"_":"A 6His-2Flag dual-tag strategy enables high-yield production of antimicrobial peptide N6 in to combat multidrug-resistant .","i":["Pichia pastoris","Klebsiella pneumonia"]}
PMID:日期:2027-04-01In response to the global threat posed by multidrug-resistant , this study established an efficient heterologous expression system for the antimicrobial peptide N6 in X-33. By employing an innovative 6His-2Flag dual-tag fusion strategy coupled with formic acid-mediated cleavage, we achieved a high-yield production of N6, with a final titer of 1.02 g/L-significantly surpassing previous reports and meeting the threshold for scalable industrial production. The expressed N6 exhibited potent and broad-spectrum antibacterial activity against clinical isolates of , with MIC values ranging from 2 to 8 μg/mL. Mechanistic studies revealed that N6 exerted rapid bactericidal and biofilm-eradicating effects through a multimodal action involving membrane disruption, metabolic interference, and induction of oxidative stress. In a murine systemic infection model, the N6 treatment significantly improved survival rates of mice to 70% and reduced bacterial burdens in key organs. This work not only identifies N6 as a promising therapeutic candidate against drug-resistant bacterial infections, but also provides a streamlined and cost-effective synthetic biology platform for the high-level production of antimicrobial peptides, thereby facilitating their clinical translation and industrial application.
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3. {"_":"Artificial gene clusters designed for simultaneous multi-gene expression enable reconstruction of metabolite biosynthesis pathways in .","i":["Trichoderma reesei"]}
PMID:日期:2027-04-01The filamentous fungus represents a highly promising chassis cell for the efficient synthesis of both protein and non-protein metabolites. Despite the availability of diverse genetic manipulation tools for , the engineering of chassis cells frequently requires coordinated regulation of multiple genes, a process that remains laborious and time-consuming with current methods. Here, we designed a modular and inducible artificial gene cluster (AGC) by repurposing functional DNA elements from the quinic acid (QA)-responsive gene cluster in . This AGC is capable of expressing up to six functional genes for the assembly of genetic circuits. Under culture conditions utilizing a mixed carbon source of glycerol and QA, both robust cellular vegetative growth and activation of the gene cluster are compatible. Moreover, we identified as a gene encoding a putative transcriptional activator that governs the expression of the gene cluster. Notably, its overexpression alone is sufficient to bypass QA-sensing induction. Leveraging this insight, we engineered a QA-independent, constitutively expressed version of the AGC. As a proof of concept, we reconstructed the biosynthetic pathways for ilicicolin H and erythritol in using the above QA-dependent and QA-independent AGCs, successfully achieving the synthesis of ilicicolin H and enhanced erythritol production in the chassis cells. In summary, our study establishes novel genetic manipulation tools for engineering chassis cells, allowing for rapid, multiplexed gene expression regulation to facilitate metabolite biosynthesis.
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4. Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment-A mini review.
PMID:日期:2027-04-01Harmful cyanobacterial blooms (HCBs) are a prominent worldwide environmental concern. Proliferation of HCBs outpaces the development of mitigation and management technologies. Recently, cyanophages began to emerge as a potential abundant resource that can be harnessed for target-specific HCB treatment. However, there exist two major technical barriers in applying cyanophage-based treatment to HCB mitigation: host specificity and lysogenic life cycle. This minireview attempts to parse documented studies that developed or applied genetic engineering approaches or technologies to tackle the barriers through expanding host range and bypassing or abolishing the lysogenic life stage. We summarized representative technical approaches that may be extrapolated from bacteriophage to cyanophage, discussed genes and pathways that regulate host binding and infection or determine the switch between lytic and lysogenic lifecycles, identified existing knowledge and technological gaps and challenges, and proposed some attainable solutions and future perspectives for this fast-growing field of engineered cyanophage-mediated HCB remediation.
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5. {"_":"Biosynthesis of vanillin glucoside in .","i":["Escherichia coli"]}
PMID:日期:2027-04-01Vanillin is a widely used flavor compound in the food, cosmetic, and pharmaceutical industries. Although microbial biosynthesis offers a green alternative to conventional chemical synthesis, challenges such as unstable heterologous enzyme expression, insufficient methyl donor supply, and product cytotoxicity limit production. Here, we engineered to produce vanillyl alcohol, vanillin, and vanillin glucoside directly from glycerol. Genomic integration of the alcohol dehydrogenase and multi-copy of the caffeic acid -methyltransferase COMT gene eliminated the need for plasmid-based expression, yielding 1.09 g/L vanillyl alcohol in shake flasks. To overcome methylation bottlenecks, we reinforced the -adenosyl-l-methionine (SAM) cycle by enhancing SAM biosynthesis, accelerating -adenosyl-l-homocysteine (SAH) hydrolysis, and establishing methionine regeneration, which increased vanillyl alcohol production to 1.95 g/L. Subsequent deletion of four endogenous aldehyde-reduction genes together with removal of heterologous enabled vanillin accumulation at 551.6 mg/L. Introduction of the glucosyltransferase UGT72E3/2 converted vanillin into vanillin glucoside, reaching 1.37 g/L in shake flasks and 2.12 g/L in a 3-L fed-batch fermenter. This work establishes a modular platform for efficient production of vanillin and its derivatives in .
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6. Directed biosynthesis of the designer peptidyl nucleoside antibiotics featuring a simplified azetidine-containing moiety.
PMID:日期:2027-04-01Fungal and fungal-like pathogen infections pose escalating threats to the global one-health, thus underscoring the urgent need to reverse this trend by accelerating the development of novel antifungal drugs. Polyoxin (POL), a group of structurally related nucleoside antibiotics, is an eco-friendly fungicide targeting fungal cell wall biosynthesis. However, the chemical diversity and therapeutic potential of this antibiotic has remained underexplored. Here, we report the rational design of a series of POL analogs that feature a simplified azetidine-containing moiety, and we have further realized the directed biosynthesis of them using an industrial POL producer as cell factory. Notably, three analogs (POL-A, POL-H, and POL-K), featuring a l-A2CA (l-azetidine-2-carboxylic acid) moiety, exhibit enhanced inhibitory activity against and even the oomycete plant pathogen . Moreover, systematic molecular docking analyses reveal that the three POL analogs can be better accommodated to the active pocket of the chitin synthases, directly contributing to the improved inhibitory activity of the designer POL analogs. The study provides the basis for the rational access of next-generation agents targeting chitin biosynthesis.
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7. Corrigendum to "Improved catalytic efficiency of P450 OleP for converting lithocholic acid into murideoxycholic and ursodeoxycholic acids through semi-rational and rational design" [Synth Syst Biotechnol 15 (2027) 94-104].
PMID:日期:2027-04-01[This corrects the article DOI: 10.1016/j.synbio.2026.05.015.].
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8. {"_":"Characterization of two key cytochrome P450 enzymes in and production of 3α,15β-dihydroxy-kaurene in yeast.","i":["Isodon amethystoides","de novo","ent-"]}
PMID:日期:2027-04-01Glaucocalyxin A (GLA), a well-known kaurene diterpenoid, has emerged as a high-value bioactive natural product in pharmaceutical research owing to its potent and broad-spectrum activities. To date, its total synthesis has proven extremely challenging due to poor yield and intricate routes, and the biosynthetic pathway of GLA remains poorly understood. Herein, we identified two enzymes, IamCYP71D761 and IamCYP706V18, in , which specifically mediate the C3α- and C15β-hydroxylation of kaurene with high stereoselectivity and regioselectivity, respectively, generating 3α,15β-dihydroxy-kaurene (), a key precursor in the biosynthesis of GLA. In addition, we established an efficient biosynthesis platform for in . Briefly, high-efficiency isozyme screening, protein engineering, increased acetyl-CoA synthesis, and copy number enhancement were applied to the kaurene biosynthesis module. Subsequently, promoter optimization, competitive pathway knockout, and electron transfer optimization were introduced, resulting in production of to 24.0 ± 0.2 mg/L in shake flask. In summary, this work highlights the pivotal roles of IamCYP71D761 and IamCYP706V18 in the heterologous biosynthesis of , offering valuable insights for the further pathway reconstruction and production of GLA, GLB, and other kaurene diterpenoids.
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9. {"_":"Ribosome engineering enhances genetic code expansion in .","i":["Saccharomyces cerevisiae"]}
PMID:日期:2027-04-01Genetic code expansion enables the site-specific installation of noncanonical amino acids (ncAAs) into proteins, but its limited efficiency in eukaryotes remains a major barrier to broader application. Here we establish a visual, plug-and-play screening platform to evolve 18S ribosomal DNA in and identify ribosomal variants that improve ncAA incorporation. The best-performing strain, designated ribo-hyper, increased ncAA-dependent GFP production by 2.9-fold relative to the wild-type rDNA strain and enhanced incorporation across distinct orthogonal aminoacyl-tRNA synthetase/tRNA pairs. Characterization of ribo-hyper showed that global translation activity and cellular growth were moderately reduced. Proteomic analysis further revealed changes in amino acid biosynthesis, translation-related proteins and stress-response pathways, indicating that the engineered ribosome reshapes cellular translation homeostasis. Perturbation of translation quality-control pathways, including the ribosome-rescue factors Dom34 and Hbs1 and the core mRNA exosome component Ski6, reduced ncAA-containing protein output, whereas disruption of ribosome quality-control factor Rqc2 had little effect. These findings support a role for ribosome rescue and associated mRNA turnover in efficient ncAA incorporation in the ribo-hyper strain. Together, our results establish eukaryotic ribosome engineering as a viable strategy for improving genetic code expansion in yeast.
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10. {"_":"Development of a new recombineering system for species.","i":["Edwardsiella"]}
10. {"_":"为物种开发新型重组工程系统。","i":["爱德华氏菌属"]}PMID:日期:2027-04-01species are important aquaculture pathogens that also cause opportunistic infections in humans, necessitating efficient genome editing tools to study their pathogenesis and develop control strategies. In this study, we identified and characterized six endogenous recombinases pairs from and its phages. Among these, the BAS_MS17 system exhibited the highest recombination efficiency in EIB202Δp. Extending homology arms from 150 bp to 200 bp improved editing efficiency by 2-fold, while the addition of Redg or Plug further enhanced recombination by 3-fold and 2.5-fold, respectively, without compromising accuracy (100%). More importantly, when applied to sdu12S, Redg or Plug improved the editing efficiency by 8-fold and 7-fold, respectively. Deletion of the phage-derived single-strand binding protein (SSB) reduced efficiency to 25% of the BAS_MS17 level, whereas expression of the endogenous RecA-family SSB (rSSB) increased recombinant yield by 5-fold, highlighting functional conservation. Furthermore, SSB proteins from heterologous hosts failed to enhance recombination efficiency. Using the optimized system, we successfully knocked out ten distinct genes, including virulence-associated loci, with editing accuracy exceeding 85%. Phenotypic analysis revealed that , but not the other tested genes, contributes to biofilm formation. Virulence evaluation results showed that , , and are critical virulence-associated factors. Collectively, this streamlined recombineering system provides a simple, rapid, and efficient genetic tool for , supporting mechanistic studies of virulence and the development of live attenuated vaccine candidates.