ZOOLOGICAL RESEARCH动物学研究
ZOOLOGICAL RESEARCH(英文缩写 ZOOL RES),ISSN 2095-8137,eISSN 2095-8137,中文译名:动物学研究 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 6.975 | Q1 |
| 2022 | 4.900 | Q1 |
| 2023 | 4.000 | Q1 |
| 2024 | 4.700 | Q1 |
| 2025 | 5.600 | Q1 |
ZOOLOGICAL RESEARCH 最新收录文献
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1. {"_":"Temperature-dependent protease LasB from PS-1 mediates enteritis and pyroptosis in .","i":["Aeromonas hydrophila","Pelodiscus sinensis"]}
1. {"_":"PS-1的温度依赖性蛋白酶LasB介导了肠道炎症和细胞焦亡。","i":["嗜水气单胞菌","中华鳖"]}PMID:日期:2026-09-22Temperature is crucial for the interaction between pathogens and hosts, especially for ectotherms, which is closely related to the occurrence of disease. Chinese soft-shelled turtle ( , ), as an economically valuable aquatic species, is also an ectothermic animal. Its aquaculture industry is severely affected by pathogens. Therein, ( ) is one of the main pathogens causing bacterial diseases in . Here, we found that temperature is essential for the pathogenicity of to . What's more, a temperature-dependent virulence factor LasB, which mediated pyroptosis of intestinal fibroblast-like cells of was identified. Through expression detection at different temperatures, it was found that the expression level of LasB was highest at 35℃. Moreover, after knockout of , the toxicity of the bacteria to intestinal fibroblast-like cells of was decreased, with reduced cell damage and lower LDH release rate associated with pyroptosis. Transcriptomic analysis revealed that LasB knockout significantly attenuated the upregulation of pyroptosis-related genes ( , , , and ) and inflammatory pathways in infected cells. After knockout of , the pathogenicity of to is significantly reduced, especially at 35℃. These results indicated that LasB played an important role in the pathogenicity of , and its virulence is closely related to temperature.
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2. Genomic insights into local adaptation of indigenous chickens.
2. 本土鸡局部适应的基因组学见解PMID:日期:2026-09-18Indigenous chickens are an essential part of biodiversity and a vital protein resource to humans, yet global warming and environmental changes pose serious threats to their survival and productivity. Therefore, assessing population adaptive capacity under shifting environments is crucial for breeding resilient animals, and guiding conservation strategies. Here, we integrated ecological and whole-genome resequencing data from 1 022 chickens from 44 Chinese indigenous populations to reveal genomic signatures of local adaptation. From 87 agroclimatic variables, we identified eight dominant environmental factors including solar radiation, precipitation, diurnal temperature range, and five landcover variables (cropland areas, water areas, trees coverage, bare ground and shrubs coverage) that shape ecological niches of indigenous chickens. Landscape and comparative genomics analyses revealed both known and novel candidate genes, such as , , , , and for the solar radiation, precipitation, diurnal temperature range, cropland areas, trees coverage and bare ground, respectively. Particularly, adaptive non-coding variants harbored in these genes exhibited spatial allelic changes across populations and acted as regulatory elements via chromatin accessibility and DNA methylation, influencing adaptation in a tissue-specific manner. Our findings underscore the rich genetic diversity of Chinese indigenous chickens and provide new insights into genomic mechanisms of local adaptation, offering valuable references for domestic animal breeding, conservation, and climate resilience.
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3. Lipopolysaccharide-induced glycolytic reprogramming drives H3K18 lactylation-mediated inflammatory injury in buffalo mammary epithelial cells.
3. 脂多糖诱导的糖酵解重编程驱动水牛乳腺上皮细胞中H3K18乳酰化介导的炎症损伤PMID:日期:2026-09-18Buffalo mastitis impairs milk production and quality, yet the underlying epigenetic mechanisms remain unclear. While LPS-induced glycolytic activation and lactate accumulation contribute to inflammation, their role in histone lactylation-mediated injury is unexplored. Our results revealed that mammary tissues from buffalo with clinical mastitis exhibited elevated LPS levels, upregulation of inflammatory markers, enhanced apoptosis, and markedly higher levels of lactate, Pankla, and H3K18la compared to healthy controls. Similarly, an LPS-induced mouse mastitis model recapitulated the inflammatory and apoptotic features observed in buffalo tissues, confirming LPS as a key driver of mammary tissue injury. In vitro, LPS treatment of buffalo mammary epithelial cells (BuMECs) upregulated proinflammatory and proapoptotic markers while downregulating antiapoptotic genes. RNA-seq and western blot analyses further revealed that LPS enhanced the expression and activity of key glycolytic enzymes, such as , , , and , thereby promoting glycolytic flux, increasing lactate production, and elevating Pankla and H3K18la levels. CUT&Tag analysis suggested that H3K18la may facilitate the recruitment of transcriptional regulators, such as , , and , potentially linking lactylation to the activation of inflammatory and apoptotic pathways. Importantly, inhibition of reduced lactate accumulation, decreased H3K18la levels, and substantially attenuated inflammatory injury in BuMECs. Together, these findings identify the HK2-lactate-H3K18la axis as a central mechanism in LPS-induced inflammatory injury in BuMECs and underscore its potential as a therapeutic target for alleviating mastitis and enhancing mammary health in dairy buffalo.
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4. {"_":"Inheritance of the epigenetic signature and reduced intermuscular bone phenotype acquired via DNA methylation editing of the 2 promoter in zebrafish.","i":["runx","b"]}
PMID:日期:2026-09-18The presence of intermuscular bones (IBs) can directly affect the economic value of aquaculture fish. Although genome editing can create IB-free fish by knocking out key IB-related genes, such as , the associated DNA sequence alterations raise food safety and health concerns, limiting its breeding applications. In this study, we used CRISPR/dCas9-mediated epigenome-editing technology targeting the 2 promoter in zebrafish to alter DNA methylation patterns without changing the DNA sequence. Our results showed that higher 2 promoter methylation patterns significantly inhibited mRNA expression levels in the recombinant plasmid. Using the CRISPR/dCas9-Dnmt7 system to enhance methylation of the zebrafish promoter, we observed a significant decrease in 2 mRNA expression levels in the F0 generation. The IBs in the 11 -16 muscle segments of the adult F0 fish were significantly shorter compared with the controls. Inbreeding of fish was used to produce F1 and F2 offspring that retained these high promoter methylation levels, along with persistent expression suppression and IB development inhibition. Transcriptome sequencing analysis suggested that increasing 2 promoter methylation levels may synergistically induce additional epigenetic modifications, potentially affecting the PPAR signaling pathway and FoxO transcription factor regulation, which appears to inhibit osteoblast proliferation and differentiation. Overall, this study demonstrates an innovative application of epigenetic editing technology for aquaculture breeding. By precisely regulating the expression patterns of key genes for economically important traits while preserving genomic DNA integrity, this approach provides a theoretical foundation and technical support for improving fish economic traits.
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5. GPR87 protects UVB-damaged skin by coordinating antioxidant, DNA repair, and anti-inflammatory responses via Nrf2 and PI3K/AKT-NF-κB pathways.
PMID:日期:2026-09-18GPR87 has been implicated in tumorigenesis, malignant progression, and remodeling of the tumor microenvironment in various cancers; however, its role in the skin remains largely unexplored, despite its abundant expression in skin. Here, we found that UVB exposure markedly downregulated GPR87 expression in human skin tissues, HaCaT keratinocytes, and mouse skin. Importantly, genetic deletion of exacerbated UVB-induced skin injury in C57BL/6 mice. In keratinocytes, RNA interference-mediated knockdown of increased reactive oxygen species (ROS) levels. Both and data further showed that GPR87 deficiency aggravated inflammatory responses and cyclobutane pyrimidine dimer (CPD) accumulation while attenuating apoptosis in skin cells. Moreover, GPR87 facilitated CPD clearance by regulating the expression and ubiquitination of XPC, a key component of the nucleotide excision repair pathway. Mechanistically, GPR87 enhances antioxidant defense by promoting Nrf2 nuclear translocation and the expression of downstream antioxidant genes. In parallel, loss of GPR87 activates the PI3K/AKT pathway and its downstream effector NF-κB. Notably, pharmacological inhibition of AKT effectively reversed the GPR87 deficiency-induced CPD accumulation and inflammatory responses. Collectively, these findings demonstrate that GPR87 protects against UVB-induced skin damage by coordinating antioxidant defense, DNA repair, and anti-inflammatory responses through Nrf2 and PI3K/AKT-NF-κB pathways, establishing GPR87 as a key endogenous factor in maintaining skin homeostasis under UVB stress. Thus, targeting GPR87 may provide a promising strategy for preventing or alleviating UVB-induced photodamage and related skin disorders.
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6. Fetal hypoxia causes oocyte oxidative stress damage via the Sirt3/Sod2 pathway and can be alleviated by nicotinamide mononucleotide.
6. 胎儿缺氧通过Sirt3/Sod2通路引起卵母细胞氧化应激损伤，并可通过烟酰胺单核苷酸缓解PMID:日期:2026-09-18Environmental hypoxia exerts detrimental effects on the reproductive capabilities of both humans and animals. A fetal hypoxia model was established in which fetal mice were kept in a high-plateau hypoxic setting from embryonic day (E) 0 to 16.5. In our previous research, we found that fetal hypoxia exposure perturbs the methylation of imprinted genes in adult sperm and causes intergenerational placental impairments in male offspring. However, the specific impacts of fetal hypoxia on the female reproductive system, particularly regarding oocyte maturation, remain poorly understood. First, we found that fetal hypoxia mice exhibited a significant reduction in the average number of pups per litter. We conducted a comprehensive analysis of the transcriptome in oocytes from the hypoxic group and investigated the metabolic alterations within the follicular microenvironment. Fetal hypoxic stress contributed to cleavage and blastocyst rate reduction and induced early apoptosis and DNA damage triggered by mitochondrial dysfunction, oxidative stress aggravation and Sirt3/Sod2 downregulation. Additionally, administration of nicotinamide mononucleotide (NMN) has been shown to prevent oocytes from mitochondrial dysfunction and developmental impairment by increasing the expression of Sirt3/Sod2 and autophagy. The number of pups per litter in fetal hypoxia mice was reduced by 57.7% compared to the control group, while NMN intervention could restore it to 73.1% of the control group. These results indicate that fetal hypoxia exposure exerts multiple potential damages to adult female reproduction, while highlighting the clinical potential of NMN supplementation as a targeted intervention to alleviate such hypoxia-associated female reproductive impairment.
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7. Regional and laminar organization of the entorhinal cortex in tree shrews based on cytoarchitecture, chemoarchitecture, and connectivity.
PMID:日期:2026-09-08The entorhinal cortex (EC) is a pivotal interface that integrates multimodal cortical and subcortical information into the hippocampal formation and distributes hippocampal output to widespread telencephalic targets. It is also one of the earliest and most severely affected regions in Alzheimer's disease (AD). The tree shrew ( ) has emerged as a promising model for AD research. However, the uncharted organization of tree shrew EC has limited its application in modeling early stages of AD. In this study, we provide a comprehensive anatomical reference for the tree shrew EC by delineating its boundaries, parcellating its medial and lateral subregions, and mapping its laminar connectivity based on cyto- and chemoarchitecture combined with tracing experiments. We found that the tree shrew EC exhibits an intermediate architecture between rodents and monkeys, mirroring its phylogenetic position and potentially links to the gradual evolution of place codes from rodents to primates. This study provides the first detailed entorhinal atlas of this species, establishing a critical foundation for future neural-tracing and electrophysiological investigations. It enhances the utility of the tree shrew in mechanistic studies of spatial coding and conscious memory, as well as a naturalistic model for incipient AD.
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8. {"_":"A horizontally acquired gene mediates insect cocoon pigmentation in the eri silkmoth, .","i":["Samia ricini"]}
8. {"_":"一个水平获得的基因介导了蓖麻蚕的茧色素沉着。","i":["蓖麻蚕"]}PMID:日期:2026-09-08Holometabolous insects make cocoons during larval-pupal metamorphosis to protect the pupal phase. The materials used for cocoon construction vary widely. Lepidopteran insects typically secrete silk to form cocoons, which display diverse colors. The eri silkworm, , is an economically important domesticated species that mostly produces white cocoons, with some varieties producing red cocoons. The enzyme kynureninase (KYNU), acquired from bacteria by horizontal gene transfer, has previously been implicated in insect coloration, while the tryptophan metabolite 3-hydroxyanthranilic acid (3-HAA) has been identified as a red pigment. However, exactly how is involved in cocoon pigmentation remains unclear. Here, we report that a horizontally transferred bacterial gene encoding regulates red cocoon formation. Metabolomic analysis revealed a high accumulation of 3-HAA in red cocoons, confirming its role as the primary pigment and associating the coloration with tryptophan metabolism. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated that is highly expressed in the silk glands and significantly downregulated in the red cocoon strain compared to the white cocoon strain. Genomic sequencing identified a 141 bp deletion in the upstream regulatory region of in the red cocoon strain compared to the white cocoon strain. Dual-luciferase assays confirmed that this deletion significantly reduced promoter activity. CRISPR/Cas9 knockout of in the white-cocoon strain resulted in mutants producing red cocoons with elevated 3-HAA content. These findings reveal that the horizontally transferred gene exhibits tissue-specific expression and regulates cocoon coloration in , illustrating that horizontal gene transfer can play an important role in regulating an insect physiological process.
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9. A novel positive feedback regulatory loop, TGF-β1/SMAD4/miR-184, controls the follicular development in sows.
9. 一种新的正反馈调节环TGF-β1/SMAD4/miR-184控制着母猪的卵泡发育PMID:日期:2026-09-08TGF-β1, the core ligand of the transforming growth factor β (TGF-β) signaling pathway, is crucial for follicular development and female fertility. However, it remains unclear whether microRNAs (miRNAs), an important class of epigenetic regulators, mediate the anti-atretic effect of TGF-β1. Here, joint transcriptomic analysis demonstrated that miR-184, an anti-atretic miRNA, was significantly elevated by TGF-β1 in sow granulosa cells (GCs). Quantitative detection, correlation analysis, luciferase reporter assays, and chromatin immunoprecipitation confirmed that TGF-β1 induces transcription in a SMAD4-dependent manner, with SMAD4 acting as a transcriptional activator that directly binds to the promoter under TGF-β1 stimulation. RNA-seq and bioinformatics analysis revealed that the target genes of miR-184 are enriched in the TGF-β pathway, with being feedback-induced by miR-184. In vivo and in vitro mechanistic analyses revealed that miR-184, a small-activating RNA (saRNA), activates transcription by binding to its promoter and forming an RNA-induced transcriptional activation complex with AGO2, CTR9, DHX9, and RNA polymerase II. Consequently, a novel positive feedback loop (TGF-β1/SMAD4/miR-184) was identified. In vitro GC and follicle culture systems validated the anti-apoptotic/atretic functions of this loop. Moreover, comparative analyses showed that the levels of TGF-β1 and miR-184 in the follicles of high-fertility sows were significantly higher than those in low-fertility sows. Our findings highlight the critical role of the interaction between TGF-β1 and miR-184 in inhibiting GC apoptosis and follicular atresia, providing potential targets for improving follicular development and sow fertility.
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10. {"_":"Exosomes-mediated molecular mechanisms underlying the transformation of ovarian tissue into a functional placental analogue in black rockfish ( ).","i":["Sebastes schlegelii"]}
PMID:日期:2026-09-08Viviparity has independently evolved multiple times in teleosts, leading to diverse modes of maternal nutrient provisioning. In black rockfish ( ), embryos gain dry weight during gestation, supported by a placental connection that facilitates maternal-fetal nutrient transfer. Although prior studies have outlined the morphology and evolutionary convergence of the black rockfish placental analogue, its full architecture and underlying molecular mechanisms remain unresolved. Here, we show that the maternal component of the placental analogue, derived from ovarian follicular tissue, consists of a vascularized outer layer and a glandularized inner layer, organized into a sac-like structure. At the molecular level, this transformation involves epithelial-mesenchymal interactions, angiogenesis, and immune responses. We observed exosome-like structures surrounding the placental analogue and isolated ovarian exosomes for characterization. These findings suggest active crosstalk between developing embryos and the maternal ovary, which may underlie the observed changes. Subsequent proteomic and transcriptomic analyses revealed that these exosomes carry diverse functional cargos, including hepatocyte growth factor b ( ) mRNA. Ovarian cells efficiently internalize exosomes, upregulating mRNA and protein expression and secretion, which in turn reprograms gene expression and promotes angiogenesis-related placentation. Together, these results elucidate the molecular underpinnings of ovarian placentation in black rockfish and offer valuable perspectives on the oviparity-to-viviparity transition in fish evolution.