JOURNAL OF BIOTECHNOLOGY生物技术杂志
JOURNAL OF BIOTECHNOLOGY(英文缩写 J BIOTECHNOL),ISSN 0168-1656,eISSN 1873-4863,中文译名:生物技术杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.595 | Q2 |
| 2022 | 4.100 | Q2 |
| 2023 | 4.100 | Q2 |
| 2024 | 3.900 | Q2 |
| 2025 | 4.000 | Q2 |
JOURNAL OF BIOTECHNOLOGY 最新收录文献
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1. Do plants used for vaccine production contribute to immunogenicity and immunoprotection beyond antigen expression?
PMID:日期:2026-11-01Plant-based systems have proved to be a versatile platform for recombinant vaccine production. Numerous species of model and agronomic edible plants have been engineered to express antigens targeting diverse pathogens. Leaves, fruits, tubers, and seeds have served as expression tissues, while subcellularly, the endoplasmic reticulum, chloroplasts, and vacuoles have been targeted to enhance antigen yield and stability. Furthermore, most of the studies have demonstrated the immunogenicity of vaccines produced and administered within plant tissue. Interestingly, certain studies of wild-type (near-isogenic and non-transgenic) plant tissues used as experimental controls consistently exhibit intrinsic immunostimulatory properties. These effects, often overlooked, include enhanced antibody production, cytokine modulation, and partial protection during pathogen challenge. Bioactive compounds such as alkaloids, flavonoids, terpenoids, glycoalkaloids, polysaccharides, glucosinolates, carotenoids, and phenolics may contribute to these responses, varying across species. Evidence from multiple animal models shows that WT plant matrices can influence immune outcomes, sometimes outperforming inert controls. Recognizing the immunomodulatory contribution of plant-derived compounds is essential for accurately evaluating plant-made vaccines and for optimizing future formulations in both human and veterinary applications.
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2. Construction, expression, and characterization of a multi-epitope protein based on envelope glycoproteins B, C, and D of Varicellovirus bovinealpha1/5 in Escherichia coli.
PMID:日期:2026-11-01Varicellovirus bovinealpha1 (BoAHV-1) and 5 (BoAHV-5) are important pathogens associated with respiratory, reproductive, and neurological disorders in cattle, remaining globally relevant since their first reports in the 1950s and 1960s. Envelope glycoproteins B, C, and D play critical roles in viral attachment and fusion, making them key targets for immune responses and promising candidates for vaccine development. This study aimed to design a multi-epitope protein using immunoinformatic approaches, incorporating highly conserved, antigenic B- and T- cell epitopes with strong predicted MHC-binding affinity from glycoproteins B, C, and D of BoAHV-1/5, followed by in silico characterization and heterologous expression in Escherichia coli. The construct was designed using bioinformatics tools, cloned into the pET-24a vector, and expressed in a prokaryotic system. The resulting protein consists of 321 amino acids, with a predicted molecular weight of 33.24 kDa, high antigenicity (1.1543) and non-allergenicity. Molecular docking analyses indicated strong interactions with bovine Toll-like receptors TLR2/6 and TLR4. The protein was successfully expressed in E. coli and detected by anti-His6x antibody in Western blot, showing the expected molecular weight (~33 kDa). It was also recognized by bovine serum containing neutralizing antibodies against BoAHV-1/5. Overall, these findings support the feasibility of the multi-epitope construct and provide a foundation for future investigations of its immunogenicity and protective efficacy studies in animal models.
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3. Combining adaptive laboratory evolution and metabolic engineering for efficient erythritol production from crude glycerol by Yarrowia lipolytica.
PMID:日期:2026-11-01Erythritol is a natural zero-calorie sweetener with potential for sustainable healthy diets. While yeasts can convert biodiesel-derived glycerol into erythritol, the low production and by-product accumulation limit its industrial commercialization. Here, we isolated different morphologically Yarrowia lipolytica mutants with higher erythritol production through adaptive laboratory evolution under hyperosmotic stress, in which mutant Z exhibited superior growth performance and membrane-related genetic variants compared to the parent strain. We then systematically engineered strain Z to optimize the flux towards erythritol by improving glycerol utilization, reducing the synthesis of competing sugar alcohols, complementing auxotrophic markers, and boosting precursor supply. After fermentation condition optimization and two-stage fed-batch fermentation in a 5-L bioreactor, the final engineered strain Z12 produced 250.76 g/L erythritol from pure glycerol and 232 g/L erythritol from crude glycerol, which is the highest reported titers for both feedstocks. This study demonstrates the effective integration of adaptive evolution with metabolic remodeling for efficient erythritol biosynthesis.
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4. Cysteine-condensed thiazolidine as an effective cysteine source for CHO cell culture.
4. 半胱氨酸缩合噻唑烷作为CHO细胞培养的有效半胱氨酸来源PMID:日期:2026-11-01Cysteine is an essential amino acid for Chinese hamster ovary (CHO) cell culture, yet its poor stability and solubility at neutral pH complicate media and feed formulation, particularly in fed-batch and intensified processes. Thiazolidines, formed by condensation of cysteine with α-ketoacids, offer enhanced chemical stability; however, there are no published applications of their use as cysteine sources. This may be due to limitations by their slow reaction kinetics and poor compatibility with industrial feed preparation. Here, we reported a rapid, high-yield, and manufacturing-compatible cysteine-to-thiazolidine condensation method using α-ketoacids naturally present in mammalian metabolism. By optimizing reaction pH, substrate concentration, and molar ratios, complete conversion was achieved within 1-2 h under near-neutral conditions, as indicated by depletion of free thiols to negative control levels. This enabled the preparation of highly concentrated and storage-stable thiazolidine solutions suitable for direct incorporation into single-feed formulations. Using this optimized approach, thiazolidines were deployed as the sole cysteine source in CHO cell culture feeds and supported cell growth, productivity and product quality comparable to established cysteine delivery approaches across multiple CHO cell lines in both standard and intensified fed-batch processes. Mechanistically, cysteine-condensed thiazolidines function as reversible reservoirs that release cysteine through non-enzymatic equilibrium, maintain low free thiol levels while ensuring sufficient intracellular cysteine availability. Together, these results demonstrate that optimized cysteine-condensed thiazolidines provide a manufacturing-compatible alternative for single-feed cysteine delivery in CHO cell culture processes.
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5. Rational design of high-productivity perfusion processes for CHO Cells: From growth inhibitory strategies to model-driven optimization.
PMID:日期:2026-11-01While perfusion culture for Chinese hamster ovary (CHO) cells offers advantages such as continuous operation and flexibility, it suffers from product loss through cell bleeding and difficulties in reaching high productivity due to sustained rapid cell growth. Growth inhibitory strategies are widely used to enhance productivity in fed‑batch processes; however, their practical implementation and comparative effectiveness in perfusion processes remain insufficiently explored. Meanwhile, process development often relies on costly trial‑and‑error approaches. Here, we systematically compared three growth inhibitory strategies in perfusion culture-low cell‑specific perfusion rate (CSPR), sodium butyrate, and mild hypothermia-with respect to cell growth, metabolism, productivity, and product quality. Genome‑scale metabolic flux sampling analysis revealed that low‑CSPR and sodium butyrate induce a convergent up‑regulation of energy metabolism, correlating with greater gains in specific productivity (q). Building on this insight, we developed a growth‑kinetic model for the combined low‑CSPR + butyrate strategy, incorporating parameter uncertainty. This model‑guided framework enabled the rational design of two distinct high‑productivity perfusion processes: a sustained mode that achieved robust long‑term stability alongside substantial productivity gains, and a high‑intensity mode that pushed q and daily volumetric titer to their maxima, with increases of up to 108.94% and 190.36%, respectively, in a model CHO cell line with a moderate baseline productivity. Our study provides a proof‑of‑concept framework for perfusion intensification, from strategy selection to rational process design.
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6. Newly isolated Corynebacterium glutamicum strain PP80 for use in high-temperature L-glutamic acid fermentation.
PMID:日期:2026-11-01High-temperature fermentation is a strategy to increase the fermentation rates and process stability while reducing cooling costs; however, metabolic heat generated during large-scale cultivations can impair cell growth and product formation. To address this bottleneck, we sought high-temperature L-glutamic acid fermentation processes using naturally thermotolerant Corynebacterium strains as alternatives to experimentally evolved strains. We isolated Corynebacterium glutamicum PP80 from soil in Thailand and evaluated its performance in penicillin-triggered L-glutamic acid fermentation at elevated temperatures. Compared with the type strain C. glutamicum KY9002 (ATCC 13032) and other isolates, including the thermotolerant species Corynebacterium suranareeae N24, PP80 maintained higher fermentation performance at 37-39°C, producing more than 40 g/L L-glutamic acid in jar fermentor cultures. PP80 also retained higher cell viability than KY9002 after penicillin treatment. Although penicillin induced membrane vesicle production in both strains, PP80 showed a smaller penicillin-associated increase in phospholipid-rich membrane vesicles than KY9002. A focused comparison of proteins associated with stress responses, cell-envelope biogenesis, and L-glutamic acid metabolism and export showed broad conservation of the canonical pathways but identified candidate differences, including an additional MscCG2-like protein in PP80. The results identify PP80 as a candidate strain for further evaluation in elevated-temperature L-glutamic acid fermentation.
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7. Fabrication of covalent immobilizers utilizing chitosan-magnesium chelating interactions.
PMID:日期:2026-11-01The chelating interactions amongst chitosan (CS) and Mg²⁺ were exploited to fabricate CS beads, with glutaraldehyde (GA) incorporated into the gelling solution to introduce the functionalities necessary for β-galactosidase (βGAL) covalent binding and also to provide additional cross-linking. The fabrication parameters of the GA/Mg-CS beads were systematically optimized by varying the concentrations of MgSO₄, GA, CS and acetic acid (AA). It was disclosed that the most efficient gelation solution comprised a mixture of 0.5% MgSO-1% GA, and that it was optimal to utilize a 2% CS solution in 0.5% AA. Structural, morphological and elemental characteristics of the beads were assessed using FTIR, SEM and EDX, respectively. The mechanical durability of the GA/Mg-CS beads was also corroborated. The GA/Mg-CS beads successfully immobilized βGAL, achieving immobilization efficiencies of up to 59.78%. Following immobilization, the βGAL optimal pH shifted from 5.3-4.9 to 4.2, whereas its optimal temperature was unchanged within the range of 56-60°C. The immobilized βGAL (iβGAL) demonstrated higher tolerance to solvents compared to its free homologue. Furthermore, the iβGAL preserved 75.25% of its initial enzymatic activity following 15 consecutive assay cycles. It also maintained 109.14% activity by the 50th storage day. Finally, the applicability of the iβGAL was demonstrated in 24 h whey-permeate lactose degradation experiments at 45 °C.
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8. Immobilization of tryptophan synthase on a magnetic bead-T4 capsid biocarrier for multi-round biocatalysis producing high-value L-tryptophan analogs.
8. 将色氨酸合酶固定于磁珠-T4衣壳生物载体用于多轮生物催化生产高价值L-色氨酸类似物PMID:日期:2026-11-01Enzyme immobilization is pivotal for industrial biocatalysis yet often suffers from activity loss, structural distortion, operational instability, and high production costs. This study presents a magnetic bead-T4 capsid biocarrier (MBTCB) platform for efficient immobilization of an engineered tryptophan synthase Pf0A9 to enable sustainable synthesis of high-value tryptophan analogs. Soc-fused Pf0A9 was immobilized onto T4 phage capsids via high-affinity Soc-capsid binding under mild physiological conditions, preserving native enzyme conformation, and combining with magnetic bead conjugation for rapid separation. The immobilized form of the enzyme, Pf0A9@MBTCB, retained near-native enzyme activity and efficiently synthesized diverse analogs. The system demonstrated exceptional stability with full activity retention over five consecutive catalytic cycles and 84.5% activity after 9-day storage at 4°C, attributed to the robust T4 capsid scaffold, spatially ordered enzyme array preventing aggregation, and ultra-stable Soc-capsid anchoring. Economically, the process utilized unpurified crude lysate, eliminated centrifugation/filtration steps via magnetic recovery, and enabled reuse of unbound enzymes and capsids. In scaled biocatalysis, Pf0A9@MBTCB produced 443 mg of L-6-Cl-tryptophan over three cycles, which was a 2.75-fold yield enhancement versus single-use free enzyme. This platform merges high catalytic efficiency, operational robustness, and cost accessibility, offering a scalable solution for green manufacturing. The modular MBTCB strategy holds broad potential for multi-enzyme cascades and continuous-flow bioreactors, advancing sustainable biocatalysis for chiral chemical synthesis.
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9. New design of a chimeric enzyme based on vanadium-dependent bromoperoxidase and glucose oxidase to highlight the microbicidal properties of the reaction products: HOBr and HOCl.
PMID:日期:2026-11-01Vanadium-dependent haloperoxidases are enzymes found in bacteria, fungi and red or brown macroalgae. They are used as a defense system for the latter by producing bromoform. The intermediate product, HOBr or HOCl, is also a microbicidal compound that can be exploited in many domains as medical tools or disinfectant sprays for example. We show that the mutation identified in Ohshiro's work, which alters substrate specificity toward chloride, is transferable to the homologous enzyme from Chondrus crispus. We then applied genetic and enzymatic engineering to design a chimera with glucose oxidase, thereby enhancing antimicrobial properties by providing a local source of HO. We used the SpyTag/SpyCatcher technology to form the chimera and obtained homogenous objects, indicating that one oligomeric form is favored. We demonstrated the release of HOBr and HOCl thanks to NADH that reacts spontaneously with it outside the active site of the enzyme. We also measured the steady-state kinetic parameters of the wild-type or modified enzymes. The formation of the chimera increased the specificity of ccVHPO1 inside the chimera, towards KBr or HO, compared to the enzyme alone with the SpyCatcher. Finally, we showed a significant increase in the microbicidal effect between a coupled enzymatic system with the glucose oxidase (the two enzymes are free in solution) compared to the chimera system, which is completely bactericidal at concentrations around 20 nM.
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10. A genome-wide coverage-based pipeline for the identification of host-derived candidate DNA biomarkers from cell-free blood.
PMID:日期:2026-11-01We have created a new data-analysis pipeline for the discovery of host-specific candidate DNA biomarkers derived from sequencing data of cell-free blood. Unlike approaches that rely on specific molecular or genetic signatures, our method leverages the coverage distribution of cell-free DNA sequences mapped to a reference genome, applying statistical analyses to identify informative short genomic regions for biomarker discovery. The pipeline is applicable to diverse diseases and can be used to analyze cell-free DNA sequences from plasma or serum to identify candidate biomarkers that are characteristic of disease states in mammals. Core functionalities were developed in Java and integrated with open-source software tools for the preprocessing of raw sequencing data, complemented by Python scripts for the machine-learning analysis and statistical validation. The pipeline is designed for HPC use and users can access the pipeline through a Galaxy workflow, which offers a user-friendly web interface for input selection prior to execution and analysis progress monitoring. Performance tests, carried out using duplicate sets of COVID-19 samples and controls, showed linear scalability of execution time with an increasing dataset size, as well as a substantial reduction in execution time through parallelized computation, whereby each HPC node is used to process the data of one chromosome. Further statistical tests confirmed the quality of the pipeline's results by showing that the set of identified candidate biomarkers remained stable across varying dataset sizes.