JOURNAL OF SEPARATION SCIENCE分离科学杂志
JOURNAL OF SEPARATION SCIENCE(英文缩写 J SEP SCI),ISSN 1615-9306,eISSN 1615-9314,中文译名:分离科学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.614 | Q2 |
| 2022 | 3.100 | Q2 |
| 2023 | 2.800 | Q2 |
| 2024 | 2.800 | Q2 |
| 2025 | 3.600 | Q2 |
JOURNAL OF SEPARATION SCIENCE 最新收录文献
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1. A Five-Dimensional "Spider-Web" Strategy for Quality Markers Identification of Traditional Chinese Medicine Compound Preparations Based on an Integrated Characteristic Network.
PMID:日期:2026-09-01Given the complex composition of traditional Chinese medicine (TCM) formulas, quality evaluation cannot rely on a single component or a limited few. Therefore, a five-dimensional quality marker (Q-marker) identification system encompassing "effectiveness, specificity, transferability, measurability, and formula compatibility" was developed and subsequently applied to Fufang Huangqi Jianpi oral liquid (FFHQJPOL) as an example. Using UHPLC-Q-TOF/MS, 62 compounds were identified in the preparation. The measurability dimension was constructed based on 17 major constituents derived from 23 common peaks in the chromatographic fingerprints of 10 batches. Serum pharmacochemistry via UHPLC-Q-TOF/MS revealed 32 prototype components absorbed into the bloodstream, thereby establishing the transferability dimension. Network pharmacology approaches were applied to construct a "component-target-disease-pathway" network, defining the effectiveness dimension. A topological network of compounds and herbs was established to depict the distribution pattern of the candidate compounds across various herbs, thereby revealing specificity. The compound compatibility dimension was established based on the function and proportional weight of its active ingredients in the formula. Ultimately, through regression area (RA) and coefficient of variation (CV) calculation, 3-hydroxy-9,10-dimethoxypterocarpan, ononin, zizyphusine, calycosin-7-O-glucoside, and sinapine were initially identified as the key Q-markers. It is worth mentioning that astragaloside IV and astragaloside II were additionally incorporated into the Q-markers system based on UHPLC-Q-TOF/MS analysis and their known pharmacological activities, ultimately establishing seven key Q-markers for FFHQJPOL. This strategy provided a multi‑dimensional integrated paradigm for Q‑markers discovery in TCM compound preparations, facilitating the transition from single‑index to system‑integrated quality control.
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2. Online Enrichment and Separation of Phthalides and Phenolic Acids From Chuanxiong Rhizoma by Reversed Migration Cyclodextrin-Modified Micellar Electrokinetic Chromatography Combined With a Three-Step Enrichment Technique.
PMID:日期:2026-09-01A sensitive three-step online enrichment strategy including large-volume sample stacking, dynamic pH junction, and sweeping was successfully developed and validated for the simultaneous online enrichment and separation of phthalides and phenolic acids in Chuanxiong Rhizoma (CX) by coupling reversed migration cyclodextrin-modified micellar electrokinetic chromatography (RM-CD-MEKC) with a diode array detector. The samples were prepared in a low-conductivity, high-pH matrix consisting of 3 mM borax buffer at pH 7.5. The background electrolyte was a high-conductivity, low-pH buffer containing 10 mg·mL sulfobutylether-β-cyclodextrin, 80 mM sodium dodecyl sulfate, and 25 mM sodium dihydrogen phosphate in 20% methanol (pH 3.5). Under the optimized conditions, four major active components (senkyunolide A, senkyunolide I, ferulic acid, and chlorogenic acid) of Ligusticum chuanxiong Hort. were efficiently separated within 17 min, achieving good resolution and 10- to 44-fold enrichment. The limits of detection ranged from 0.09 to 0.25 µg·mL while the limits of quantification ranged from 0.31 to 2.24 µg·mL. The relative standard deviations for intra-day and inter-day precision were all below 3.45%, demonstrating good precision and method stability. Overall, the proposed RM-CD-MEKC combined with a three-step enrichment technique provides a simple, rapid, and environmentally friendly enrichment approach for the simultaneous determination of phthalides and phenolic acids in CX samples.
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4. Mechanistic Origins of Pressure Reduction, Apparent Efficiency Gain, and Enhanced Detector Response in Radial Flow Stream Splitting Chromatography.
PMID:日期:2026-09-01Radial flow stream (RFS) splitting has been reported to reduce operating pressure and apparent theoretical plate height (increasing column efficiency) while increasing detector response relative to conventional high-performance liquid chromatography column operation. However, the mechanisms responsible for these effects have remained unclear. Here, pressure measurements, computational fluid dynamics, and reduced-order outlet models were used to examine how radial outlet partitioning in the new end fitting alters column behavior. Most of the measured pressure reduction arose from post-column effects, although a smaller in-column contribution remained. Simulations showed that the radial outlet disrupted the conventional outlet-flow funnel and preferentially captured wall-adjacent flow. When the outlet models were applied to a published model of radial column heterogeneity, apparent column plate height decreased by approximately 19% and peak height increased by approximately 18%, consistent with typical experimental results. Experimental sensitivity improvements also persisted under detector conditions that were independent of flow rate. These results indicate that the reported benefits under RFS splitting mode arise from a combination of post-column pressure effects and selective sampling of the radial solute distribution.
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5. Identification of Wild-Simulated Ginseng Powder, Cultivated Ginseng Powder, Cultivated Ginseng Decoction Pieces, and Baoyuan Decoction Containing Wild-Simulated Ginseng and Cultivated Ginseng by UHPLC-Q-TOF-MS/MS and Non-Targeted Metabolite Profiling.
5. 采用UHPLC-Q-TOF-MS/MS和非靶向代谢物谱分析鉴定林下参粉、栽培人参粉、栽培人参饮片及含林下参和栽培人参的保元汤PMID:日期:2026-09-01Wild-simulated ginseng (linxiashen, LXS) powder, farm-grown ginseng (yuanshen, YS) powder, and farm-grown ginseng decoction pieces (YSDP), as well as the Baoyuan decoction prepared from them (BYD-LXS, BYD-YS, and BYD-YSDP) by boiling, exhibit certain metabolic differences in their chemical composition. To elucidate the holistic molecular profile and differences, a reversed-phase UHPLC-Q-TOF-MS metabolomics strategy with an ACQUITY HSS T3 stationary phase was applied to characterize and discover differential chemical markers. In total, 293, 663, 311, and 645 compounds (including 22, 13, 24, and 28 rare ginsenosides) were annotated in LXS/YS, BYD-LXS/BYD-YS, YS/YSDP, and BYD-YS/BYD-YSDP respectively. Based on PCA and OPLS-DA, 87, 222, 95, and 168 chemical markers (including 8, 7, 13, and 3 rare ginsenosides) were annotated in LXS/YS, BYD-LXS/BYD-YS, YS/YSDP, and BYD-YS/BYD-YSDP decoctions, respectively. Secondary metabolites such as triterpene saponins were found to be present at higher levels in LXS decoction and BYD-LXS decoction. The amount of components eluted from YSDP decoction was significantly higher than that from the decoction of YS powder. In addition, Support Vector Machine and Back Propagation Neural Network models with feature selection nested within cross-validation were constructed to classify LXS/YS, BYD-LXS/BYD-YS, YS/YSDP, and BYD-YS/BYD-YSDP decoctions, achieving 100%, 100%, 100%, and 94.44% classification accuracies, respectively. This study provides a basis for the quality assessment of ginseng and Baoyuan decoction, as well as for the development and utilization of rare ginsenosides.
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6. Molecular Networking-Based Annotation of Tuberostemonine Isomers Using MS/MS Spectral Similarity.
PMID:日期:2026-09-01Stemona alkaloids possess multiple stereogenic centers, giving rise to structurally diverse isomeric forms that remain challenging to differentiate by conventional mass spectrometric approaches. In this study, six tuberostemonine isomers were annotated and discriminated using LC-MS/MS in combination with molecular networking-guided spectral similarity analysis. Based on their MS/MS spectral features, these isomers were classified into two distinct groups. Gaussian fitting further revealed characteristic differences in fragmentation energy profiles and relative fragment-ion intensities, enabling refined differentiation of closely related isomeric alkaloids. The relative abundances of these isomers were subsequently compared across commercial "Bai-Bu" herbal materials. These results provide experimental evidence and an MS-based analytical strategy for the identification, discrimination, and quality evaluation of isomeric Stemona alkaloids.
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7. Room-Temperature Synthesis of Anionic Covalent Organic Framework Monolithic Materials for Efficient Capturing of Berberine Hydrochloride.
PMID:日期:2026-09-01An anionic covalent organic framework (COF) was constructed using 2,5-diaminobenzenesulfonic acid as an ionic building unit. The incorporation of sulfonate functionalities endows the framework with permanent anionic sites, thereby enabling selective ion-exchange interactions with cationic species. The adsorption performance toward berberine hydrochloride (BBH) was systematically investigated by optimizing pH, ionic strength, adsorbent dosage, extraction time, and elution conditions. Rapid adsorption equilibrium was achieved within 10 min, and the kinetic data were well described by a pseudo-second-order model, indicating a chemisorption-dominated process. Under optimal conditions (pH 11, 303 K), the maximum adsorption capacity reached 1082.4 mg g. The material maintained high adsorption efficiency in the presence of competing cations, demonstrating pronounced selectivity arising from electrostatic interactions. Moreover, it retained over 90% of its initial adsorption capacity after five adsorption-desorption cycles, confirming its structural stability and recyclability. These results highlight the effectiveness of introducing ionic functionalities into COF architectures to achieve high-capacity and selective adsorption of alkaloid compounds, providing a viable platform for the separation and analysis of BBH in complex herbal matrices. The design and synthesis of novel COF porous adsorption materials will play a crucial role in the separation and analysis of natural products.
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8. Prediction of Elution Profiles in Countercurrent Chromatography With a Linear Loss of Stationary Phase.
PMID:日期:2026-09-01Countercurrent chromatography (CCC) is a liquid-liquid separation method that is predominantly used for the preparative isolation of natural products. Key indicators for the development of CCC methods are the partition coefficient (K value) between the two liquid phases and the properties of the chosen biphasic solvent system. Based on this information, it is possible to predict elution volumes and peak widths with the probabilistic model for immiscible separations and extractions (ProMISE). However, a common challenge in reliably predicting CCC elution profiles is a persistent loss of stationary phase (i.e., bleeding), which leads to a decrease in the percentile share of stationary phase (S value) in the column as the separation progresses. In this study, two carotenoids were isolated and used as test compounds to investigate the effect of bleeding with the n-hexane/benzotrifluoride/acetonitrile (20:7:13, v/v/v) solvent system. After showing that the stationary phase bleeding rate was linear (and not exponential), experiments were carried out both in head-to-tail and in tail-to-head mode. Next, a simple calibration method was developed to predict elution volumes and peak widths with the ProMISE2 software in CCC separations with linear bleeding. Finally, based on the collected data, a method was developed to accurately predict the elution volume and peak width during a (long) CCC run, particularly for analytes with comparatively high elution volumes.
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9. Systematic Evaluation of Partition-Dominated Retention in Hydrophilic Interaction Liquid Chromatography (HILIC) Under Varying Aqueous Components of the Mobile Phase: Revisiting the HILIC Column Test Scheme.
PMID:日期:2026-09-01The robustness of a test for characterizing hydrophilic interaction liquid chromatography (HILIC) columns against variations in aqueous mobile-phase composition was evaluated using mobile phases of acetonitrile/aqueous mixture (9:1, v/v) in the pH range of 3.6-6.8 of the aqueous part. Ten columns (commercially available and home-made columns) were examined under five mobile phases differing in anion species (formate or acetate), NH concentration and salt concentration. The specific hydrophilicity of the columns was assessed via the retention factor of uridine (k(U)), and the relative retention characteristics (i.e., retention selectivity: α) were evaluated using structure-related probe pairs. The k(U)-values exhibited low variability under low-salt conditions (20 mM in the aqueous part; CVs = 0.01-0.08), but higher variability under high-salt conditions (100 mM in the aqueous part; CVs = 0.03-0.18). Under comparable pH conditions (ammonium formate at pH 4.5 vs. ammonium acetate at pH 4.7), k(U)-values were consistently higher in the former, indicating that differences in NH concentration affect hydration at the stationary-phase interface and thereby influence retention. This suggests that k(U), commonly used as an indicator of hydrophilic retention, can be affected significantly by partition interactions that are controlled by ionic concentration rather than the pH value of the mobile phase. In contrast, the characteristic α-values primarily associated with solute retention via partitioning exhibited high robustness (CVs ≤ 0.03). Notably, α(k(U)/k(2'-deoxyuridine)) values correlated with the estimated phase ratio, suggesting that it reflects the effective volume of the hydrated layer on the surface of the packing materials. Furthermore, the relationships between α(k(1-β-D-arabinofuranosyl uracil)/k(U)) and α(k(vidarabine)/k(adenosine)) indicated that nucleobase‑dependent interactions contribute significantly to stationary‑phase retention. Overall, these results demonstrate that each k(U) and α, particularly those associated with partition-dominated retention, exhibit generally high robustness within this test framework and provide a basis for more robust and transferable HILIC column characterization.
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10. Determination of Enantiomers of Deschloroketamine and Its Metabolites in Wistar Rat Brains.
PMID:日期:2026-09-01Deschloroketamine (DXE) is a dissociative new psychoactive substance whose enantiomers and metabolites may exhibit distinct pharmacological and toxicological profiles. Reliable analytical methods enabling the chiral determination of DXE and its metabolites in biological matrices are thus essential. In this study, we evaluated supercritical fluid chromatography coupled to tandem mass spectrometry (SFC‑MS/MS) and capillary zone electrophoresis with ultraviolet detection (CZE‑UV) for the enantioselective analysis of DXE and its major metabolites in rat brain tissue. SFC using polysaccharide‑based chiral stationary phases provided baseline separation of the hydroxylated and demethylated metabolites, but severe peak distortion of DXE enantiomers prevented their quantification. Therefore, quantitative analysis was performed by CZE employing carboxymethyl‑β‑cyclodextrin as a chiral selector and a high‑sensitivity Z‑shaped detection cell. Analysis of brain tissue samples obtained following high-dose administration revealed near equimolar concentrations of the DXE enantiomers and a higher concentration of (R)-norDXE relative to (S)-norDXE. In this particular case, the combined use of SFC‑MS/MS and CZE‑UV provided a practical workflow for the chiral profiling of DXE and its metabolites in complex biological matrices.