LAB ON A CHIP芯片实验室
LAB ON A CHIP(英文缩写 LAB CHIP),ISSN 1473-0197,eISSN 1473-0189,中文译名:芯片实验室 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 7.517 | Q1 |
| 2022 | 6.100 | Q1 |
| 2023 | 6.100 | Q1 |
| 2024 | 5.400 | Q1 |
| 2025 | 6.300 | Q1 |
LAB ON A CHIP 最新收录文献
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1. Vascularized glioblastoma-on-a-chip reveals microglia-mediated regulation of tumor invasion and stemness.
PMID:日期:2026-09-25Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor in adults, characterized by rapid progression and poor prognosis. Central to GBM pathobiology are glioma stem cells (GSCs), which preferentially localize within the perivascular niche (PVN) and sustain tumor growth, invasion, and therapeutic resistance through dynamic interactions with the surrounding PVN microenvironment. Notably, microglia (MG), the brain-resident immune cells, represent a substantial fraction of the GBM PVN and play a pivotal role in establishing an immunosuppressive tumor microenvironment (TME). However, the mechanisms by which MG interacts with the brain PVN and how this, in turn, regulates GSC behavior remain poorly understood. To systematically investigate these complex cellular interactions, herein we engineered a compartmentalized human GBM PVN-on-a-Chip (GPoC) model featuring a perfusable brain microvascular network surrounding a tumor core composed of patient-derived GSCs and a stromal region containing brain-resident MG. The presented biomimetic 3D model recapitulates the structural and functional features of the native PVN, enabling well-controlled experimental interrogation and spatial visualization of tumor-immune-vascular crosstalk. Through invasion assay, real-time live imaging, immunofluorescent staining, and secretome profiling, it was evident that the GSC cells exhibited enhanced invasion in the presence of both MG and vasculature. Furthermore, pharmacological intervention studies revealed attenuated GB3 migration under tri-culture conditions, highlighting that the migratory phenotype of the tumor cells is, in part, driven by MG. Overall, the engineered GPoC platform serves as a physiologically relevant human brain tumor model for dissecting MG-mediated mechanisms and advancing therapeutic targeting of the GBM microenvironment.
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2. Improving CTC isolation by controlling orbit engagement in inertial microfluidics.
PMID:日期:2026-09-24Inertial microfluidics provides a label-free strategy for isolating circulating tumor cells (CTCs) from blood by exploiting size-dependent lateral migration in microchannels. However, straight-channel devices are often optimized using the final equilibrium position, even though target-cell loss can arise from incomplete or poorly reproducible migration before outlet collection. Here, we show that heteroclinic orbit engagement provides a predictive design criterion for improving CTC scale separation in a rectangular co-flow inertial microchannel. A computational model predicted size-dependent heteroclinic orbits and lateral migration velocities, which were experimentally validated using beads under controlled inlet flow-split conditions. Increasing buffer confinement shifted the sample-buffer interface closer to the sidewall, promoted earlier orbit engagement, improved agreement between measured and simulated velocity profiles, and reduced upstream trajectory variability. Larger CTC scale particles engaged their orbits earlier and migrated more reproducibly, while smaller white blood cell (WBC) scale particles remained laterally displaced from the larger particle focusing region. Biological validation with PANC1 and A549 cells showed that the 1 : 6 : 1 flow-split configuration increased separation efficiency to 99.9 ± 0.2% for PANC1 and maintained high separation efficiency of 99.2 ± 0.9% for A549 cells, although its effect on total recovery was cell line dependent. In an intraductal papillary mucinous neoplasm (IPMN) patient sample, representing a premalignant pancreatic lesion that may progress to invasive pancreatic ductal adenocarcinoma (PDAC), CTC target outlet partitioning was 67% under 1 : 1 : 1 and 100% under 1 : 6 : 1, within the limits of the detected cell numbers. These results establish orbit engagement as a mechanistic design principle for improving clinically relevant CTC enrichment without changing device geometry, total flow rate, or labeling strategy.
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3. Mechanically programmable whispering gallery mode microlasers for information encryption.
PMID:日期:2026-09-24Microlasers featuring multiple emission degrees of freedom offer a unique physical platform for information encryption. However, conventional microlasers provide limited access to intrinsic mechanical properties of microcavities, leaving this mechanical dimension largely untapped for information encryption. Here, we propose mechanically programmable whispering gallery mode (WGM) microlasers to address this limitation. The microlasers were fabricated by inkjet printing a dye-doped solution on a superhydrophobic surface. The mechanical properties of the liquid microcavities can be programmed by changing the glycerol concentration. Ultrasound was applied to stimulate the microlaser, and the resulting dynamic laser spectra were used to read out the mechanical properties encoded in the microcavity. We found that the mechanical properties of microlasers establish a physically secure dimension orthogonal to conventional optical methods. We further employed these mechanically programmable WGM microlasers for information encryption. We envision that hybrid optical-mechanical encryption can be achieved through integration with conventional optical methods.
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4. Optimization of lipid nanoparticle components and microfluidic parameters for intramuscular circRNA delivery with reduced hepatic off-targeting.
PMID:日期:2026-09-22Circular RNA (circRNA) represents a promising class of RNAs for sustained protein expression and vaccine applications. However, current lipid nanoparticle (LNP) platforms remain poorly optimized for efficient intramuscular circRNA delivery, as they are primarily designed for the delivery of small interfering RNA and linear messenger RNA with strong off-target expression in the liver. In this study, based on the clinical benchmark SM-102 LNP, we systematically optimized lipid components and microfluidic parameters for better intramuscular circRNA delivery. Through and screening of a variety of phospholipids and sterols, we identified a 1,2-dioleoyl--3-phosphoethanolamine (DOPE)- and cholesterol-based formulation, which demonstrated improved storage stability and enhanced intramuscular transfection while minimizing hepatic off-target expression. Using ovalbumin as a model antigen, we proved that this formulation-based vaccine elicited more balanced Th1/Th2 immune responses with reduced liver toxicity. Together, this study successfully develops an optimized formulation with reduced hepatic off-target effects, which holds great promise for the intramuscular delivery of circRNA-based vaccines and therapeutics.
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5. Leaching behaviour of stereolithography 3D-printed microfluidics.
PMID:日期:2026-09-22Stereolithography (SLA) 3D printing has become a standard tool to produce microfluidic structures, thanks to advancements in both 3D printer technology and resin materials. Used across many applications, SLA-printed microfluidics are often exposed to a variety of solvents, giving rise to potential leaching from the printed structure (in the form of unreacted resin components) that can potentially interfere with device functionality. In this study, we investigated the leaching behavior of SLA-printed structures from both external surfaces (under static conditions) and microfluidic surfaces (under flowing conditions). We focused on how leaching is affected by factors such as resin and solvent type, post-processing, fluidic architecture, and internal flow rates. Using a combination of gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) analysis, we identified 15 chemical species consistently found in leachate samples from 4 commercial resins, in the form of monomers and photoinitiators. We found that increased post-processing led to an overall reduction in leaching, with monomers being more affected than photoinitiators. In no case was leaching eliminated. For leaching from microfluidic channels, we found that light treatment did not result in any reduction in leachate levels, which can be attributed to the absorption of light in the external regions of the structure. These findings highlight the importance of thorough post-processing, careful solvent selection, and optimal fluid flow conditions to improve the chemical stability and biocompatibility of SLA-printed devices in fluidic applications.
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6. {"_":"A deep learning-enhanced surface acoustic wave platform for contact-free biomechanical characterization of embryos.","i":["in situ"]}
PMID:日期:2026-09-22Understanding the mechanical strength of embryos is essential for evaluating their viability in developmental biology. This study introduces a novel biosensor designed for , non-contact mechanical characterization. The platform offers several unique contributions, including: (1) a microfluidic system combining SAW and deep learning for the automated quantification of embryonic mechanical strength; (2) a quantitative fitting model relating SAW power to acoustic radiation force for precise mechanical loading; (3) the first quantitative atlas of embryonic mechanical thresholds, achieved through the systematic measurement of two key parameters: the apparent maximum compressive stress (MCS) causing developmental arrest and the apparent ultimate compressive strength (UCS) leading to embryonic rupture. The results demonstrate consistent embryonic strengthening from zygote to pharyngula stages, with MCS increasing from 8.113 kPa to 11.407 kPa and UCS from 10.256 kPa to 14.219 kPa. This automated platform advances the understanding of embryonic structural integrity for developmental biology and tissue engineering.
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7. Millifluidic triMEA organoid platform with stretchable microelectrode arrays for parallel organoid electrophysiology under standardized perfusion.
PMID:日期:2026-09-21Organoids enable rigorous studies of neurodevelopment and disease, yet existing platforms often compromise between precise microenvironment control and reliable electrophysiological measurement. The challenge of achieving uniform perfusion alongside multi-well volumetric neural recordings limits parallel functional characterization and technical reproducibility across samples. Here, we present the triMEA millifluidic platform, which integrates three stretchable microelectrode arrays within a single device and delivers uniform perfusion across three independent wells. A single inlet supplies identical perfusion to each well, which stabilizes flow patterns and equalizes mechanical stimuli between chambers. This design facilitates simultaneous, independent electrophysiological recordings from three organoids within a single experimental setup. Using human midbrain organoids as a proof-of-concept biological model, we provide computational and experimental validation of the fluidic profiles and demonstrate electrophysiological recordings across all three wells under continuous perfusion. This platform establishes a reproducible and standardized foundation for parallel organoid interfacing.
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8. Magnetic bead induces surface tension change at the gas-solid-liquid interface to manipulate the reciprocating flow of a droplet for rapid immunoassay of viruses.
PMID:日期:2026-09-21Droplet manipulation provides tools for medical diagnostics, cell culturing, and drug screening. Among droplet manipulation techniques, magnetic manipulation enables transportation, mixing and separation of droplets, and thus has garnered broad attention for rapid immunoassay. Typically, magnetic manipulation requires a ferrofluid or micron-sized magnetic particles as droplet actuators. However, ferrofluids may contaminate the droplet, and magnetic particles may trap unbound molecules (, viruses, antigens) within their aggregation gaps. These disadvantages decrease the accuracy of immunoassay. Here, we use a millimeter-sized magnetic bead to manipulate a droplet by inducing a surface tension change at the gas-solid-liquid interface. The manipulation of each droplet solely requires a single magnetic bead that releases no solvent or contaminant, eliminating the trapping of unbound molecules in the gaps between aggregated magnetic beads. We further design a chip and its complementary miniaturized analyzer to manipulate the reciprocating flow of droplets, and develop a rapid immunoassay method to detect Sendai virus and pneumonia virus of mice within 10 min. This method can detect target viruses in serum with a true positive rate of 100% (6/6) and a true negative rate of 93.3% (28/30). This work demonstrates great performance in droplet manipulation and the rapid immunoassay of viruses, with good potential to serve as a universal platform for detection and medical diagnosis.
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9. Hydrogels and microfluidics: toward more physiologically relevant organ-on-a-chip models.
PMID:日期:2026-09-18Hydrogel-integrated organ-on-a-chip (OoC) models have emerged as a promising platform for recapitulating the complexity of native human tissues. This review highlights the role of hydrogels as tissue-specific extracellular matrix (ECM) mimics, focusing on the physicochemical properties that regulate cellular behaviour and tissue function in three-dimensional (3D) systems. We also explore the contribution of microfluidic systems in further enhancing physiological relevance by integrating multiple microenvironmental cues, including dynamic biochemical gradients, biophysical stimuli, tissue architecture, temporal regulation, 3D cellular arrangements, and ECM incorporation. Following a discussion of different hydrogel-microfluidic integration strategies, along with their associated engineering challenges and design considerations, we examine how the synergistic integration of hydrogels with microfluidics enables the development of advanced OoC models that more faithfully reproduce native tissue organisation and physiology. These advances make hydrogel-integrated OoC models well suited to a broad range of applications, including studying fundamental biology, disease modelling, drug development, personalised medicine, and multi-organ system, helping bridge the gap between animal studies, traditional studies, and human physiology.
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10. A human iPSC-based perfusable and membrane-free neurovascular unit-on-chip connecting brain organoids to the blood-brain barrier.
PMID:日期:2026-09-18The blood-brain barrier (BBB) protects the central nervous system by restricting entry of harmful blood-borne factors, but this selectivity actively limits delivery of therapeutics to the brain. Because BBB function is shaped by dynamic interactions within the neurovascular unit (NVU), particularly between brain endothelial cells, pericytes, and astrocytes, there is a need for human-relevant models that capture both barrier properties and neurovascular crosstalk in a controlled setting. Here, we present a novel human induced pluripotent stem cell (iPSC)-based NVU-on-chip that couples a perfusable, vessel-like BBB compartment to an adjacent open-top chamber housing a human brain organoid (hBO) a membrane-free, hydrogel-based interface. This design enables co-culture of BBB and parenchymal components in close proximity while allowing direct cell-cell interactions at the barrier-tissue boundary without an artificial porous membrane. Using this platform, we demonstrate stable on-chip co-culture and show that hBO-derived astrocytes can sprout, migrate toward the BBB compartment and establish direct contact with endothelial cells, recapitulating the NVU structure. This system offers a human-specific framework for modeling NVU biology in health and disease and for evaluating BBB penetration together with downstream effects of candidate therapeutics on hBO.