Pesticide Biochemistry and Physiology农药生物化学与生理学
Pesticide Biochemistry and Physiology(英文缩写 PESTIC BIOCHEM PHYS),ISSN 0048-3575,eISSN 1095-9939,中文译名:农药生物化学与生理学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.966 | Q1 |
| 2022 | 4.700 | Q1 |
| 2023 | 4.200 | Q1 |
| 2024 | 4.000 | Q1 |
| 2025 | 4.800 | Q1 |
Pesticide Biochemistry and Physiology 最新收录文献
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1. Repellent and insecticidal activities of secondary metabolites from Praxelis clematidea against Solenopsis invicta (Hymenoptera: Formicidae).
PMID:日期:2026-09-01Praxelis clematidea is a high-risk invasive species with potent insecticidal and repellent properties. This study isolated and identified its bioactive constituents against the red imported fire ant, Solenopsis invicta. Through bioassay-guided fractionation using a digging-based repellency assay, an active oily mixture (I-[3]) was obtained, with a minimum repellent effective dose (MRED) of 0.0125 mg/g. Notably, caryophyllene oxide, a major component (22.3%), showed stronger repellency (MRED = 0.00156 mg/g) than DEET (MRED = 0.00625 mg/g). Friedelin (MRED = 0.0625 mg/g), stigmasterol, and 4',5,6,7-tetramethoxyflavone (each MRED = 0.125 mg/g), from moderately active fractions, exhibited only moderate activity. Caryophyllene oxide was selected for further evaluation. In behavioral bioassays, it significantly reduced S. invicta nesting activity for at least 15 days, reduced foraging ants within 1 h, and prolonged food transport time throughout the experiment. Moreover, in field tests, its repellency was comparable to DEET. In addition to its repellent effects, caryophyllene oxide also showed rapid contact toxicity (LT₅₀ = 1.19 h at 0.1 mg/cm), albeit slightly less potent than DEET (LT₅₀ = 0.558 h). Enzyme assays revealed that caryophyllene oxide exhibited concentration- and time-dependent effects: at low concentrations, acetylcholinesterase (AChE) and carboxylesterase (CarE) showed initial activation followed by inhibition, unlike glutathione S-transferase (GST), which remained consistently activated; at high concentrations, both were rapidly inhibited, with GST inhibition likely due to exceeded detoxification capacity. Overall, these results indicate that P. clematidea contains active compounds against S. invicta, with caryophyllene oxide emerging as a promising dual-action (repellent and contact insecticidal) agent for S. invicta management.
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2. DDT biotransformation by a delta-class glutathione S-transferase underlies tolerance in honey bees.
PMID:日期:2026-08-01Bees are key global pollinators but face long-term exposure to pesticide residues. Although the western honey bee (Apis mellifera) shows tolerance to the persistent organic pollutant DDT, the underlying molecular basis remains unclear. Glutathione S-transferases (GSTs) are important detoxification enzymes in bees, yet direct evidence for their involvement in pesticide metabolism remains limited. In insects, Delta- and Epsilon-class GSTs are typically associated with xenobiotic detoxification. However, the A. mellifera genome encodes only a single Delta-class GST, AmGSTD1, and lacks the Epsilon class, suggesting that AmGSTD1 may play an important role in xenobiotic detoxification in honey bees. Here, we combined genetic, biochemical, and computational analyses to investigate the role of AmGSTD1 in DDT detoxification. Silencing of AmGSTD1 in A. mellifera increased susceptibility to DDT stress, whereas transgenic expression of AmGSTD1 in Drosophila melanogaster conferred increased DDT tolerance. Enzyme kinetics and HPLC analyses demonstrated that recombinant AmGSTD1 protein catalyzed the biotransformation of DDT to DDE, while molecular docking further supported the interaction between AmGSTD1 protein and DDT. AmGSTD1 protein was also associated with alleviation of oxidative stress under DDT exposure. Furthermore, the A170V mutation was identified as an important factor affecting the detoxification activity of the AmGSTD1 protein. Overall, these findings provide functional evidence that AmGSTD1 protein contributes to DDT detoxification through both xenobiotic biotransformation and oxidative stress regulation. This study improves our understanding of the molecular mechanisms underlying pesticide tolerance in honey bees.
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3. ABCG and ABCH transporters at the interface of cuticular lipid transport and insecticide handling.
3. 表皮脂质转运和杀虫剂处理界面上的ABCG和ABCH转运蛋白PMID:日期:2026-08-01ATP-binding cassette (ABC) transporters are widely associated with insecticide resistance, yet the physiological links between endogenous transport and xenobiotic handling remain incompletely resolved for ABCG and arthropod-associated ABCH subfamilies. Unlike many canonical full ABC transporters, ABCG and ABCH proteins usually have reverse half-transporter topology and require dimeric assembly, creating substrate-recognition spaces that can support lipid movement, pigment precursor routing, cuticular barrier formation, and chemical-response phenotypes. Here, we synthesize structural, physiological, and toxicological evidence from insects and mites to evaluate how these transporters may connect cuticular lipid homeostasis with insecticide handling. Recent structural work on Tribolium castaneum TcABCH-9C supports an ATP-coupled squeeze-pump model for hydrophobic substrates, including ceramide-like lipids and fenoxycarb. This model provides an important mechanistic framework, but its direct support is currently limited to one ABCH system and should not be generalized to all ABCH orthologs or to ABCG transporters without comparative validation. We distinguish three experimentally separable interaction modes: competitive occupancy, spatial blockade, and functional uncoupling. Because direct proof that endogenous lipids and insecticides compete within the same transporter cycle remains limited, we propose an evidence-tier framework that integrates structural, biochemical, physiological, and genetic validation. Finally, we discuss how RNA interference, CRISPR/Cas9, and structure-guided perturbation can support functional testing while remaining constrained by delivery, specificity, field feasibility, and non-target arthropod safety.
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4. SfIML-1 interacts with integrin β5 to mediate fungal conidial adhesion and immune activation in Spodoptera frugiperda.
PMID:日期:2026-08-01Soluble pattern-recognition receptors are essential for detecting pathogen-associated molecular patterns, yet how they cooperate with membrane-associated receptors to enhance immune recognition and cellular responses remains poorly understood. Here, building on our previous identification of SfIML-1 (Spodoptera frugiperda immulectin-1) as a C-type lectin that recognizes both hemocytes and fungal conidia to enhance cellular immune responses, we investigated its receptor association and immune functions in S. frugiperda. Recombinant SfIML-1 induced the expression of multiple antimicrobial peptide genes in hemocytes and fat body, indicating broad activation of innate immunity. Transcriptomic profiling of SfIML-1-overexpressing Sf9 cells revealed extensive changes in genes associated with immunity, phagocytosis-related processes, and cytoskeletal organization. Notably, an integrin β subunit gene was significantly upregulated. Genome-wide analysis identified five α- and five β-integrin genes in S. frugiperda, establishing the first systematic integrin repertoire in this species. Yeast two-hybrid assays demonstrated a specific interaction between SfIML-1 and integrin β5, and domain mapping showed that the loop2 region of the carbohydrate-recognition domain is important for association with the β-tail region of β5. Fluorescence colocalization analyses further supported the association of SfIML-1 with β5 at the cell membrane. Functionally, overexpression of β5 or its extracellular/β-tail domains enhanced SfIML-1-mediated adhesion of conidia to Sf9 cells, whereas β5 silencing reduced adhesion. Collectively, these results support a role for SfIML-1 within a β5-associated immune pathway that promotes conidial adhesion and immune activation, providing new insight into how soluble pattern-recognition receptors cooperate with membrane-associated receptors during insect innate immunity and highlighting potential targets for sustainable pest management.
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5. Cell autophagy promotes nucleopolyhedrovirus infection in the fall armyworm.
PMID:日期:2026-08-01Pathogens, such as nucleopolyhedroviruses (NPVs), are promising biological control agents for lepidopteran pests to protect crops. However, a major drawback, their field application is limited by slower virulence compared with chemical insecticides. Modulating host pathways to enhance viral infectivity offers a potential strategy for improving NPV-based biocontrol. In this study, the expression level of NADH:ubiquinone oxidoreductase core subunit S8 (Ndufs8) was upregulated in the insect midgut following Spodoptera frugiperda NPV (SfNPV) infection. Silencing Ndufs8 impaired mitochondrial function, increased oxidative stress, and activated autophagy, but had no significant adverse effects on larval development and survival. The Ndufs8-autophagy cascade subsequently promoted SfNPV replication in insects. Co-feeding larvae with SfNPV and nanocarrier-delivered double-stranded RNA targeting Ndufs8 (dsNdufs8) accelerated insect death compared with the virus alone, demonstrating the enhanced virulence. Furthermore, bacterially expressed short hairpin RNA against Ndufs8 (shNdufs8), followed with nanocarrier delivery, achieved effective gene silencing and increased insect mortality comparable to synthetic dsNdufs8, supporting the potential field application for scalable RNA delivery. Our findings elucidate the function of a host responsive gene in the virus-host interaction. The combined use of dsNdufs8/shNdufs8 with SfNPV highlights a practical and scalable strategy to integrate RNA interference with pathogens to improve the biocontrol efficacy against insect pests.
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6. Burkholderia cepacia NB-13: Biocontrol efficacy and medium-driven metabolic regulation.
PMID:日期:2026-08-01Fusarium oxysporum, the primary pathogen causing lily root rot, severely impairs lily yield and quality, threatening the sustainable development of the lily industry. Conventional chemical control strategies are associated with drawbacks such as pesticide residues, environmental pollution, and pathogen resistance, emphasizing the urgent need for eco-friendly biological control strategies. In this study, an antagonistic bacterial strain Burkholderia cepacia NB-13 was isolated from healthy lily rhizosphere tissues, which exhibited 80.76% inhibition rate against F. oxysporum mycelial growth and was identified as B. cepacia through morphological and molecular verification. Whole-genome sequencing was performed to predict antifungal metabolites, while integrated transcriptome analysis and targeted pyrrolnitrin quantification elucidated the regulatory mechanism underlying differential antifungal substance production, with a significantly higher pyrrolnitrin yield in King's B Medium supported by the upregulation of prnA biosynthetic genes and ED pathway activation. In vitro and field experiments indicated that the strain achieved an in vitro control efficacy of 91.66%. Application of B. cepacia NB-13 combined with conventional field management delivered excellent biocontrol efficacy, raising the survival rate of lily to 89.58% after field treatment. The strain also showed good compatibility with organic fertilizers. Microbial diversity analysis indicated that B. cepacia NB-13 regulated the rhizosphere microenvironment without altering α-diversity. Collectively, our findings demonstrate that B. cepacia NB-13 possesses excellent biocontrol potential against F. oxysporum-induced lily root rot, providing a promising candidate for green biological control in agricultural production.
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7. Regional heterogeneity in the VGSC-based knockdown resistance to pyrethroids among Aedes albopictus populations from southern West Bengal, India.
PMID:日期:2026-08-01Aedes albopictus, a competent vector of multiple arboviral diseases and a globally expanding invasive species, poses a rising public health challenge. Reduced efficacy of the traditional chemical control due to evolving resistance limits effective outbreak management. Susceptibility test of the regional Ae. albopictus populations sampled from 16 districts of West Bengal, India, against pyrethroid insecticides- Permethrin and Deltamethrin followed by characterization of the VGSC partial segments were done. The mean mortalities against Permethrin and Deltamethrin were 92.10 ± 1.45 and 96.10 ± 1.24 respectively with significant spatial variation in the mortalities and Knockdown times among the districts' populations. Molecular analysis of the partial VGSC sequences revealed amino acid substitution at positions: 1016 (Valine to Glycine), 1022 (Alanine to either Serine or Proline), 1520 (Threonine to Isoleucine) and 1534 (Phenylalanine to Cysteine). Analysis of Molecular Variance (AMOVA) of the Domain II and III sequences showed partitioning of the genetic variation within populations accounting for 63.98% and 69.53% of the total variance whereas among population, variation contributed 36.02% and 30.47%, respectively. The overall fixation index values of 0.3601 and 0.3047, respectively, were significant (p < 0.01). Haplotype network map identified 27 and 28 haplogroups for Domain II and III, respectively with distinct phylogeographical pattern of distribution. Deviation of the allele frequencies from the Hardy Weinberg equilibrium parabola may reflect ongoing selection pressure favoring kdr alleles. Molecular docking results suggested likely reduced binding affinity of the selected pesticides with the VGSC mutants. The study revealed spatial variation of resistance patterns for Ae. albopictus across Southern West Bengal and highlights the need for region specific and evidence-based vector control strategies.
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8. Biocontrol potential and antifungal mechanism of Streptomyces angustmyceticus GD7-29 against Fusarium oxysporum f. sp. cubense tropical race 4.
PMID:日期:2026-08-01Fusarium wilt of banana, especially caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is the most devastating disease limiting global banana production. With no effective chemical control and only limited resistant cultivars available, sustainable alternatives are urgently needed. Biocontrol is increasingly considered as an environmentally friendly strategy for managing this disease. In this study, an antagonistic strain, designated GD7-29, was isolated from healthy banana rhizosphere and identified as Streptomyces angustmyceticus based on its morphological, physiological, biochemical, and molecular characteristics. The strain exhibited 70.1% inhibition against Foc TR4 and exhibited broad-spectrum antifungal activity. In pot experiments, GD7-29 achieved 50.8% biocontrol efficacy against Fusarium wilt and promoted plant growth through potassium and phosphate solubilization, protease production, and siderophore production. Genomic analysis revealed 33 biosynthetic gene clusters and multiple functional genes potentially associated with its biocontrol activity. Both the culture supernatant and volatile organic compounds (VOCs) produced by GD7-29 markedly suppressed Foc TR4. Metabolomic profiling identified 1409 differentially abundant metabolites and revealed an enrichment of secondary metabolites. GC-MS analysis detected five VOCs, among which butanoic acid was identified as a compound exhibiting both antifungal and plant growth-promoting activities in subsequent bioassays. Furthermore, GD7-29 upregulated the expression of defense-related genes in banana. This study suggests that S. angustmyceticus may represent a promising biocontrol agent for sustainable management of Fusarium wilt in banana production.
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9. Contribution of amino acid substitutions in CYP51A and CYP51B to pefurazoate resistance in Fusarium fujikuroi.
PMID:日期:2026-08-01Rice bakanae disease, caused by the fungal pathogen Fusarium fujikuroi, results in significant yield reductions in global rice production. Pefurazoate, one of the sterol 14α-demethylase inhibitors (DMIs), has been used to control rice bakanae disease. However, pefurazoate-resistant isolates have also been reported in Japan. The F490V substitution in FfCYP51A or S312T/F511S substitutions in FfCYP51B were found in field isolates with pefurazoate resistance. In the present study, we aimed to confirm the contribution of these amino acid substitutions to pefurazoate resistance; toward this, we created a V-type mutation at the 490th codon in FfCYP51A from an F-type isolate using a gene-editing technique. S/F- and T/S-types at the 312/511th codons in FfCYP51B, created from an S/S-type isolate in a previous study, were also used. A sensitivity test for pefurazoate indicated that F490V in FfCYP51A or S312T/F511S in FfCYP51B independently conferred pefurazoate resistance to F. fujikuroi. Notably, S312T/F511S in FfCYP51B was revealed to confer positive cross-resistance between prochloraz and pefurazoate. The increased expression of FfCYP51A and FfCYP51B in the pefurazoate-treated amino acid substitution mutants suggests the presence of a system regulating FfCYP51A and FfCYP51B expression in response to FfCYP51B activity in F. fujikuroi. These results demonstrate that F490V in FfCYP51A and S312T/F511S in FfCYP51B contribute to pefurazote resistance in F. fujikuroi, although additional mechanisms may also modulate fungicide sensitivity. We also developed the polymerase chain reaction-restriction fragment length polymorphism assay for F490V in FfCYP51A and S312T/F511S in FfCYP51B to manage DMI resistant isolates of F. fujikuroi.
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10. Antifungal mechanisms of Trichoderma-derived zinc oxide nanoparticles against Botrytis cinerea: Evidence from ultrastructural and biochemical analyses.
PMID:日期:2026-08-01Grey mold caused by Botrytis cinerea poses a significant threat to Hydrangea cultivation worldwide and requires the development of sustainable and effective control measures. This study explored the potential of biologically synthesized Zinc Oxide nanoparticles (T-ZnO NPs) as an eco-friendly alternative to conventional fungicides and evaluated their antifungal efficacy along with plant health impact. The ZnO NPs were synthesized using Trichoderma spp. and characterized using multiple analytical techniques. The DLS and XRD analysis revealed a uniform size distribution (20-40 nm with spherical morphology). Furthermore, SEM imaging showed agglomerated particles, while EDX confirmed Zn and O as the primary elements. FTIR spectroscopy indicated the presence of protein capping agents and ZN-O bonds. In vitro studies demonstrated significant antifungal activity, 72% growth inhibition against Botrytis cinerea. Greenhouse trials showed that dose-dependent effects with 0.5 mg/mL T-ZnO NPs treatment resulted in remarkable improvement in plant growth parameters, including 23.7% increase in leaf number, 31.1% in leaf diameter, 30.1% in shoot length, 39.9% in root length, as compared to the control. The disease severity index was decreased by 46.3% while the CO assimilation rates, stomatal conductance, and transpiration rates were successfully revived (∼100%, 85.7%, and 83.3%) by T-ZnO NPs under stress. Plant physiological analysis revealed an enhanced chlorophyll content (39.6%) and modulated stress enzymes (superoxide dismutase peroxidase, catalase, and malondialdehyde) activity. DAB and HDCFDA staining of leaf showed that the plants treated with nanoparticles showed no oxidative damage. This study highlights biosynthesized ZnONPs as an eco-friendly alternative for Botrytis cinerea management, offering potential in sustainable agriculture.