BIOTECHNOLOGY ADVANCES生物技术进展
BIOTECHNOLOGY ADVANCES(英文缩写 BIOTECHNOL ADV),ISSN 0734-9750,eISSN 1873-1899,中文译名:生物技术进展 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 17.681 | Q1 |
| 2022 | 16.000 | Q1 |
| 2023 | 12.100 | Q1 |
| 2024 | 12.500 | Q1 |
| 2025 | 14.100 | Q1 |
BIOTECHNOLOGY ADVANCES 最新收录文献
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1. Bioproduction of α,ω-oxyfunctionalised alkanes.
1. α,ω-氧官能化烷烃的生物生产PMID:日期:2026-10-03The oxidation of n-alkanes into higher-value products, such as diols and diacids, is facilitated by various wild-type and engineered microorganisms. Depending on the substrate chain length, several distinct enzymatic systems are involved in achieving highly selective diterminal oxidation. In this review, we comprehensively summarise the enzymatic pathways responsible for the conversion of alkanes to α,ω-diols and α,ω-diacids and present strategies in the field of enzyme engineering, metabolic engineering and process optimisation aiming to increase the efficiency of these bioconversions. Furthermore, we examine integrated strategies for the conversion of diacids into diols and highlight recent advancements in this dynamic field. Finally, we discuss future research directions and key bottlenecks that must be addressed to achieve efficient n-alkane valorisation, whether derived from petroleum or sustainable alternatives such as chemically recycled plastics and plant-based renewable resources.
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2. Engineering defensins: Heterologous expression strategies from genetic engineering to synthetic biology.
2. 工程化防御素：从基因工程到合成生物学的异源表达策略PMID:日期:2026-09-29Defensins are a structurally diverse class of host defense peptides with broad-spectrum antimicrobial and immunomodulatory activities. Their broader applications in biomedicine and biotechnology, however, remain constrained by limited natural availability and difficulties associated with heterologous production, including low yields, host toxicity, inefficient folding, and loss of biological activity. In this review, we examine defensin production from an integrated engineering perspective, emphasizing the interplay between expression regulation, host physiology, and molecular design. We establish a multidimensional engineering framework that integrates genetic engineering, synthetic biology, and AI-driven design strategies to improve defensin production and functional optimization. In this context, synthetic biology provides a framework for programmable regulation of gene expression and host metabolism. Recent advances in artificial intelligence further enable data-driven prediction and redesign of defensin sequences and structures, extending optimization beyond naturally occurring sequence space. By integrating these complementary approaches, defensin engineering can progressively shift from empirical optimization toward rational and quantitatively informed design. This framework provides a conceptual basis for improving the production, functional stability, and manufacturability of defensins and for guiding the development of scalable production strategies.
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3. Environmental stress drives dynamic remodeling of the plant condensatome.
3. 环境胁迫驱动植物凝聚体组的动态重塑PMID:日期:2026-09-22Phase separation, which drives the formation of dynamic and reversible membraneless biomolecular condensates, has emerged as a novel molecular strategy for adaptation to environmental stresses. In this review, we (i) summarize how this process enables rapid and precise signal integration and regulation, (ii) integrate recent advances in the study of plant biomolecular condensates, and (iii) systematically summarize the mechanisms of stress-induced phase separation. We discuss how plants utilize phase separation to orchestrate molecular responses to abiotic stresses, as well as biotic stresses such as pathogen invasion. Furthermore, we provide a focused analysis of the potential roles of endogenous plant molecules in modulating phase separation processes. Finally, we address the key questions and challenges in the field and propose a "plant condensatome" research roadmap integrating multi-omics approaches including genomics, transcriptomics, and proteomics to comprehensively map the composition, dynamic properties, and environmental responsiveness of plant condensates. We firmly believe that interdisciplinary integration to deeply resolve the mechanisms of phase separation will provide a new theoretical foundation for understanding plant environmental adaptation strategies and advancing molecular breeding for stress-resistant crops.
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4. A critical review on different strategies to enhance mass transfer in methanotrophic bioreactors.
4. 关于强化甲烷氧化菌生物反应器传质的不同策略的批判性综述PMID:日期:2026-09-22Methanotrophs are bacteria with the ability to directly utilize methane as substrate to produce different useful biomaterials (e.g., lipids, proteins, bioplastics, methanol). However, due to the solubility and mass transfer limitations of methane, the methanotroph biotechnology remains almost entirely in laboratory/research scale. To address this, studies in recent years have focused on increasing the methane volumetric mass transfer coefficient (ka) through modifications of bioreactor design and operations. This review critically analyzed these advances, aiming to identify the most suitable strategy for methane bioconversion. The modifications were categorized, i.e., mechanical agitation, feed gas introduction system, and cultivation medium modification [including addition of a non-aqueous phase (NAP)], and their effectiveness was related to ka and/or productivity metrics (PM) (i.e., yield, elimination capacity, and remediation efficiency). Studies that determined both ka and PM were assessed using a new metric (∆PM/∆ka, with ∆ as the difference between implemented strategy and control). This metric, which was independently determined for each study, allowed cross-study comparison of the effectiveness of the different modifications/strategies. A positively high ∆PM/∆ka indicates greater emphasis on PM improvement (or biological performance) over ka enhancement (or physical attributes) of the bioreactor system. While majority of strategies result in a positive ∆PM/∆ka, two-phase partitioning bioreactors (TPPBs) exhibited the highest potential (i.e., highest ∆PM/∆ka). The effectiveness of this strategy relies on the homogeneous dispersion of the added NAP. Although TPPB operation is more complex than conventional bioreactors, this review suggests that future research should consider TPPB strategies to address its complexity and economic viability.
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5. Designing gene expression with MPRA and AI: A new paradigm for synthetic and therapeutic regulation.
5. 利用MPRA和人工智能设计基因表达：合成与治疗调控的新范式PMID:日期:2026-09-22Precise and scalable control of gene expression across biological contexts remains a fundamental challenge in synthetic biology and therapeutic development, and massively parallel reporter assays (MPRAs) have emerged as a transformative platform for systematically interrogating cis-regulatory elements (CREs) and enabling high-throughput and quantitative dissection of sequence-dependent transcriptional activity to address this challenge. Beyond their original role in empirical characterization, the convergence of barcoded MPRA technologies with deep learning-based modeling has substantially expanded their impact, facilitating predictive inference and the de novo design of synthetic regulatory elements with programmable behavior. In this review, we synthesize recent advances in barcoded MPRA platforms and AI-driven modeling frameworks for the rational engineering of CREs with tunable transcriptional strength and specificity. We further highlight cutting-edge applications in microbial synthetic biology, mRNA therapeutics, and cell-specific gene regulation, emphasizing the role of barcoded MPRA in supporting data-efficient and cross-context generalizable design. Finally, we discuss current technical and conceptual limitations and future directions toward safe, programmable, and clinically relevant regulatory control systems, outlining a roadmap for next-generation cis-regulatory engineering and gene therapies.
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6. Enterobacteriaceae for succinic acid biomanufacturing: metabolic blueprints, systems-level engineering and future perspectives.
PMID:日期:2026-09-22Succinic acid (SA) is a key C4 platform molecule with wide industrial relevance, including bio-based polymers and solvents. Current reviews largely emphasize traditional producers (e.g., Actinobacillus, Mannheimia, and Basfia) and rarely synthesize progress in Enterobacteriaceae. Here, we close this gap by providing a systems-level review of SA biomanufacturing in Enterobacteriaceae, focusing on non-model genera (Enterobacter, Klebsiella, Citrobacter) that combine fast growth, facultative anaerobiosis, broad feedstock utilization, and high engineering amenability. We outline the metabolic blueprint underpinning SA formation, highlighting the reductive TCA branch, anaplerotic CO₂ fixation (PEPC/PEPCK), the glyoxylate shunt, and redox constraints that govern carbon partitioning. We then summarize recent advances in strain development, including pathway rewiring, stronger carboxylation, adaptive laboratory evolution, and dynamic control, that increase SA titers, yields, and productivities. Finally, we discuss downstream processing and techno-economic bottlenecks, biosafety and regulatory considerations, and emerging directions including division-of-labour consortia and circular-carbon integration for scalable, carbon-efficient SA biorefineries.
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7. Decoding microbial fructosyltransferases: Structural insights, molecular engineering, and biotechnological applications.
7. 解码微生物果糖基转移酶：结构见解、分子工程与生物技术应用PMID:日期:2026-09-22Fructosyltransferase (FTF), classified within glycoside hydrolase families GH32 and GH68 of the GH-J clan, plays an indispensable catalytic role in the biosynthesis of fructooligosaccharides (FOS) and fructan polymers (such as inulin and levan). These enzymes specifically catalyze fructosyl transfer reactions, constructing structurally diverse carbohydrates through β-(2 → 1) or β-(2 → 6) glycosidic linkages: FOS (linear oligosaccharides with DP 2-10 and a terminal glucose) and fructan polymers (high-molecular-weight polysaccharides). The resulting products not only exhibit excellent physicochemical properties but also demonstrate multiple physiological benefits including prebiotic activity, dietary fiber functionality, and regulation of glucose/lipid metabolism, showing broad application prospects in functional foods and pharmaceutical fields. This review systematically summarizes the microbial source diversity, three-dimensional structural characteristics, substrate recognition mechanisms and the molecular basis for the diversity of catalytic products of microbial FTF, including levansucrase, inulosucrase and β-fructofuranosidase. Focusing on enzyme molecular engineering strategies such as directed evolution, rational design, and semi-rational design, this review clarifies the intrinsic relationship between the catalytic properties of FTF and the structural and functional characteristics of their products, and discusses their application value in the synthesis of functional carbohydrates. This paper provides important theoretical support for the fundamental research of FTF and the development of precision synthesis technologies for FOS and fructans.
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8. IBISBA: Digitalisation to empower biotechnology and drive biomanufacturing.
8. IBISBA：数字化赋能生物技术并推动生物制造PMID:日期:2026-09-22The European Union is currently engaged in an economic transition towards a circular bioeconomy that is expected to combine more sustainable growth with greater industrial sovereignty and resilience. Consequently, European competitiveness increasingly depends on the ability of advanced technologies, such as biotechnology, to provide the basis for manufacturing goods. These range from fuels and chemicals to biobased materials, advanced food ingredients, and a range of biopharmaceuticals. However, unlocking the potential of enabling technologies for the circular bioeconomy is a major undertaking. It involves harnessing the power of two game-changing technologies, biotechnology and advanced digital technologies (especially AI). To achieve this, it is imperative for Europe to bring together its knowledge, expertise, and infrastructure assets and to promote the concept of data federation. In this regard, Europe is fortunate to have European research infrastructures (RI) that provide ideal frameworks to achieve this. Here, a vision of how e-Infrastructures, such as the one embedded in the Infrastructure for Biotechnology Services for Biomanufacturing (IBISBA), can contribute to the digitalisation of biotechnology and catalyse the growth of biomanufacturing is presented. By advancing asset sharing, interoperability, federated modelling, and data-driven innovation, such RI can significantly accelerate both scientific discovery and industrial application.
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9. Branched-chain amino acid biosynthetic platforms in prokaryotic cell factories: metabolic engineering and value-added chemical biomanufacturing.
9. 原核细胞工厂中的支链氨基酸生物合成平台：代谢工程与高附加值化学品生物制造PMID:日期:2026-09-21Branched-chain amino acids (BCAAs) are recognized not only as industrially important fermentation products but also as versatile biosynthetic platforms for the production of branched-chain value-added chemicals. The characteristic branched carbon skeletons of BCAAs, together with their metabolically versatile intermediates, enable the biosynthesis of a broad spectrum of value-added chemicals, including branched-chain α-keto acids (BCKAs), alcohols, and carboxylic acids. This review systematically examines the metabolic basis of BCAA biosynthesis and the features underpinning its platform functionality, followed by a comprehensive overview of metabolic engineering strategies for constructing efficient BCAA-producing prokaryotic cell factories. Particular emphasis is placed on bacterial production platforms, especially Escherichia coli and Corynebacterium glutamicum, in which engineering carbon flux through enhanced glucose uptake, expanded pyruvate availability, reinforced biosynthetic flux, regulated cofactor supply, and improved product export. Advances in enzyme engineering are also discussed, with four complementary strategies highlighted for expanding the branched-chain chemical repertoire: enhancement of catalytic activity, improvement of substrate selectivity, engineering of cofactor utilization through NADH regulation, and optimization of enzyme expression. Finally, current challenges and future directions are discussed, and an engineering framework is proposed for the rational development of BCAA platform-based biomanufacturing toward an expanded repertoire of branched-chain chemicals.
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10. Steering ecological interactions in microbial chain elongation: functions and impacts of carbon- and iron-based materials.
10. 调控微生物链延伸中的生态相互作用：碳基和铁基材料的功能与影响PMID:日期:2026-09-19The increasing accumulation of organic waste and the demand for renewable chemicals highlight the production of medium-chain fatty acids (MCFAs) as an effective strategy for waste valorization. Microbial chain elongation (CE) converts short-chain fatty acids (SCFAs) into MCFAs. However, its efficiency in mixed cultures is frequently limited by unstable carbon flow, restricted electron transfer, and shifts in functional microbial communities. While exogenous materials are increasingly applied to regulate CE, their mechanisms of action have not been as systematically reviewed as traditional process optimization strategies. This review examines how material-mediated CE enhances MCFA production by facilitating metabolic pathways, directing microbial enrichment, and promoting electron transfer. Specifically, the discussion focuses on carbon-based materials, iron-based materials, and engineered composites, detailing their role in electron shuttling, interfacial redox buffering, microbial attachment, and functional microbial community assembly. Furthermore, we summarize material design principles, their enhancement effects, and the key factors governing operational stability in practical mixed-culture systems. Rather than focusing solely on the enrichment of single functional microbes, this review emphasizes the application of exogenous materials to direct microbial community assembly and enhance interfacial microbe-material interactions. These mechanisms provide a basis for understanding and regulating caproate selectivity during organic waste conversion. Finally, by incorporating economic and environmental considerations, we propose strategies for scaling up material-assisted CE from the laboratory to practical applications. Ultimately, this review builds a framework connecting material properties, microbial ecology, and process efficiency, facilitating the selective conversion of organic waste into MCFAs.