APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY应用生物化学与生物技术
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY(英文缩写 APPL BIOCHEM BIOTECH),ISSN 0273-2289,eISSN 1559-0291,中文译名:应用生物化学与生物技术 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.094 | Q3 |
| 2022 | 3.000 | Q3 |
| 2023 | 3.100 | Q2 |
| 2024 | 3.300 | Q2 |
| 2025 | 3.500 | Q2 |
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 最新收录文献
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1. Immobilization of Heavy Metals by Microbial Extracellular Polymeric Substances: Key Mechanisms, Driving Factors, and Research Advances.
PMID:日期:2026-10-01Microbial extracellular polymeric substances (EPS) are increasingly recognized as promising biogenic matrices for heavy metal immobilization, but their role is still frequently interpreted through an adsorption-centered perspective. This interpretation limits a mechanistic understanding of why EPS-rich systems often show greater stability and adaptability than conventional biosorbents under complex wastewater conditions. This study proposes to view EPS-mediated heavy metal immobilization as a coupled and adaptive process that integrates rapid adsorption, selective coordination, precipitation, biomineralization, biofilm protection, and microbial regulation. Evidence from recent studies indicates that EPS immobilize heavy metals through ion exchange, electrostatic attraction, coordination complexation, direct precipitation, and metabolically induced biomineralization, while these pathways are jointly governed by EPS composition, functional-group distribution, spatial stratification, pH, salinity, redox state, nutrient balance, metal stress, and quorum sensing. A key point of this study is that it attempts to go beyond describing what EPS do and to explain how EPS convert transient metal capture into more stable immobilization by linking extracellular chemistry, microbial metabolism, and biofilm-scale organization. This framework suggests a different interpretation of prior studies: high adsorption capacity alone should not be treated as a universal performance indicator; instead, EPS performance should be evaluated by capacity, selectivity, post-binding stability, regeneration potential, and tolerance to mixed-metal and saline wastewater conditions. By critically comparing mechanisms, performance ranges, and engineering trade-offs, this review provides a more analytical basis for controllable EPS production, functional optimization, composite design, and resource-oriented wastewater treatment. EPS should be regarded not merely as passive biosorbents, but as adaptive biological interfaces with strong potential for sustainable and scalable heavy metal remediation.
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2. {"_":"Green Synthesis and Characterization of SiO Nanoparticles Using Origanum majorana Leaf Extract and Evaluation of its Biological Potential.","sub":["2"]}
PMID:日期:2026-10-01Silica nanoparticles (SiO₂ NPs) are versatile nanomaterials with tunable physicochemical properties and excellent biocompatibility, making them suitable for biomedical applications. In this study, a green synthesis approach was employed using Origanum majorana leaf extract as a natural reducing and stabilizing agent to fabricate biofunctionalized SiO₂ nanoparticles. The novelty of this work lies in utilizing a phytochemically rich yet underexplored plant source for synthesizing silica nanoparticles and systematically evaluating their multifunctional biological properties. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, FTIR, XRD, FESEM, EDX, and DLS analyses, confirming the formation of amorphous, high-purity SiO₂ nanoparticles with Si-O-Si and Si-OH functional groups. FESEM analysis revealed particle sizes of 50-200 nm, while DLS indicated a hydrodynamic diameter of 298.3 nm (PDI = 0.496). The nanoparticles exhibited significant antioxidant activity (96.7% DPPH scavenging at 10 µg/mL), concentration-dependent antimicrobial effects, notable anti-inflammatory activity (IC₅₀ = 165 µL), and moderate cytotoxicity against L929 fibroblast cells (IC₅₀ = 112.06 µg/mL). This study presents an eco-friendly strategy for producing biofunctionalized silica nanoparticles with promising applications in nanomedicine and antimicrobial therapy.
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3. Mangiferin as a Polyphenolic Scaffold for Enzyme Targeted Molecular Regulation of Carbohydrate Hydrolyzing Enzymes in Diabetes Management.
PMID:日期:2026-10-01Mangiferin, a bioactive xanthonoid predominantly present in mango, exhibits significant therapeutic potential in metabolic disorders, particularly diabetes mellitus. The present study was undertaken to compare the inhibitory potential of mangiferin with the standard drug acarbose against the carbohydrate hydrolyzing enzymes α-glucosidase and α-amylase using an integrated biochemical and in silico approach. Mangiferin was quantified in pulp and peel using LC-MS/MS analysis, revealing significant varietal variation, with Zardalu pulp (3.80 ± 0.2 mg/kg DW) and Dasheri peel (7.99 ± 0.2 mg/kg DW) showing the highest concentrations. Biochemical profiling demonstrated higher phenolic and flavonoid content in mango peels compared to pulp, indicating their potential as rich sources of bioactive compounds. Molecular docking analysis was performed using an AutoDock based workflow followed by residue-residue interaction analysis to evaluate ligand-protein interactions. Molecular docking analysis revealed strong binding affinity of mangiferin with α-glucosidase (-7.1 kcal/mol) and α-amylase (-8.4 kcal/mol), in comparison to standard drug acarbose. Interaction analysis showed stable hydrogen bonding, hydrophobic interactions and π-π stacking with key catalytic residues, suggesting effective inhibition of carbohydrate hydrolyzing enzymes. Structural validation using Ramachandran plot confirmed the reliability of protein models. These findings indicate that mangiferin exhibits a comparatively stronger binding affinity towards α-amylase than acarbose and may serve as a promising natural antidiabetic agent. The integration of biochemical and in silico findings highlights mango, as a promising source of natural antidiabetic compounds. However, further in vitro and in vivo studies are required to validate its therapeutic applicability.
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4. Structure-Based Design and Evaluation of Coumarin-Derived CDK4 Inhibitors for Non-Small Cell Lung Cancer: An Integrated Computational Study.
PMID:日期:2026-10-01Lung cancer remains the leading cause of cancer related mortality worldwide, and non-small cell lung cancer (NSCLC) accounts for approximately 85% of cases. Dysregulation of the Cyclin-Dependent Kinase 4-Cyclin D3 complex promotes uncontrolled cell proliferation and represents an attractive therapeutic target in NSCLC. In this study, an integrated computational workflow comprising pharmacokinetic filtering, molecular docking, molecular dynamics (MD) simulations, and MM/PBSA binding free-energy calculations was employed to identify putative coumarin-derived CDK4 inhibitor candidates from a virtual library of 30,176 compounds. The docking protocol was validated using the co-crystallized CDK4/6 inhibitor abemaciclib, yielding a redocking RMSD of 1.38 Å and a docking score of - 9.86 kcal·mol⁻. Following Lipinski and QikProp filtering, four lead compounds, 4-methylesculetin, 3-acetamidocoumarin, esculetin, and daphnetin, were selected for detailed investigation. Docking analysis identified 4-methylesculetin (- 8.63 kcal·mol⁻) and 3-acetamidocoumarin (- 9.10 kcal·mol⁻) as the top-ranked ligands. Triplicate 200 ns MD simulations demonstrated stable protein-ligand complexes characterized by persistent active-site interactions, favorable conformational stability, and sustained hydrogen-bond occupancy. MM/PBSA calculations further supported the favorable binding energetics of 4-methylesculetin (ΔG_bind = - 22.99 ± 3.31 kcal·mol⁻) and 3-acetamidocoumarin (ΔG_bind = - 22.36 ± 2.07 kcal·mol⁻). Integrated evaluation of docking affinity, dynamic stability, hydrogen-bond persistence, binding free-energy profiles, and conformational behavior identified 4-methylesculetin as the most balanced computational lead candidate. Collectively, these findings establish coumarin-derived scaffolds as promising starting points for the development of next-generation CDK4-targeted therapeutics and provide a strong computational foundation for future experimental validation in NSCLC.
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5. Potential Role of METTL14 in Modulating Prostate Cancer Proliferation and Glycolysis Through m6A Modification of LINC01138.
PMID:日期:2026-10-01This study aimed to investigate the role of methyltransferase-like 14 (METTL14) in regulating proliferation and glycolysis in prostate cancer (PCa) through m6A modification of LINC01138. The expression of METTL14 and LINC01138 was analyzed in PCa tissues and cell lines (PC-3 and 22Rv1 cells). Gain- and loss-of-function experiments were performed to evaluate their effects on cell proliferation, migration, invasion, apoptosis, and glycolysis. m6A-related assays were conducted to examine the interaction between METTL14 and LINC01138. In addition, a xenograft mouse model was established to assess the in vivo role of METTL14 in PCa progression. METTL14 was significantly downregulated in prostate cancer and its low expression correlated with advanced TNM stage and distant metastasis. Functional experiments showed that METTL14 overexpression suppressed proliferation, migration, invasion, and glycolytic activity in PCa cells, whereas METTL14 knockdown produced the opposite effects. Mechanistically, METTL14 promoted the m6A modification of LINC01138, leading to its downregulation. Rescue experiments further demonstrated that LINC01138 overexpression reversed the inhibitory effects of METTL14 on tumor progression and glycolysis. In vivo, METTL14 overexpression reduced tumor growth and LINC01138 expression in xenograft models, consistent with the in vitro findings. These findings suggest a potential role of the METTL14-LINC01138 axis in prostate cancer progression and glycolysis. Further studies are warranted to elucidate the precise downstream mechanisms linking LINC01138 to glycolysis and malignant behavior.
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6. Green Synthesis of Silver Nanoparticles Using the Extract of Adenosma caeruleum R.Br.: Evaluation of Antibacterial, Antifungal and Antioxidant Activity.
PMID:日期:2026-10-01Silver nanoparticles (AgNPs) have attracted considerable attention due to their remarkable antimicrobial and antioxidant properties. In this study, AgNPs were synthesized through a green and sustainable approach using Adenosma caeruleum R.Br. extract as both reducing and stabilizing agents. The effects of key synthesis parameters, including AgNO₃ concentration, extract to precursor ratio, temperature, pH, and reaction time, were systematically optimized to achieve efficient nanoparticle formation. Comprehensive characterization using UV-Vis, FT-IR, XRD, DLS, zeta potential, TEM, and EDS analyses confirmed the successful synthesis of predominantly spherical and crystalline AgNPs with particle sizes mainly ranging from 20 to 50 nm and a Zaverage hydrodynamic diameter of 47.5 ± 1.4 nm. Despite a relatively broad particle size distribution, the nanoparticles exhibited good colloidal stability, as evidenced by a highly negative zeta potential (-���75.2 ± 1.3 mV). The biosynthesized AgNPs demonstrated notable antioxidant activity against ABTS radicals, with an IC₅₀ value of 49.8 ± 0.14 µg/mL. In addition, significant antimicrobial activity was observed against both bacterial and fungal strains. At a dose of 30 µL, inhibition zones of 21.0 ± 0.18 mm (Escherichia coli), 14.0 ± 0.13 mm (Bacillus cereus), 22.0 ± 0.17 mm (Salmonella typhimurium), 14.0 ± 0.19 mm (Staphylococcus aureus), and 12.0 ± 0.12 mm (Candida albicans) were recorded, with Gram-negative bacteria generally exhibiting greater susceptibility. The minimum inhibitory concentration (MIC) was determined to be 1.25 mg/mL for all tested microorganisms. Overall, the results demonstrate that A. caeruleum-mediated AgNPs possess antibacterial, antifungal, and antioxidant activities and highlight the potential of this indigenous medicinal plant as a sustainable resource for the green synthesis of functional nanomaterials with prospective biomedical and environmental applications.
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7. Conversion of Rice Husk into Silicon Carbide Nanowires for Photocatalytic and Electrocatalytic Applications.
PMID:日期:2026-10-01Silicon carbide (SiC) nanowires with rhombohedral structure, length of longer than 20 μm and diameter of less than 100 nm, respectively were prepared under Ar atmosphere at 1250 ℃ for 180 min using rice husk as the raw materials. The band gap of the rice husk-derived SiC nanowires (RH-SiC) is 2.05 eV and RH-SiC can be utilized as visible-light photocatalysts for organic pollutants removal. For rhodamine B (RhB) and methylene blue (MB) removal using 25 mg RH-SiC, 20 mL RhB and MB solution (0.01 g·L) can be removed with solar light irradiation for 40 min and 55 min, respectively. RH-SiC can also be utilized as efficient electrode materials for simultaneous detection of Hg, Pb and Cd. Three stripping peaks are located at - 0.74 V, - 0.54 V, and + 0.71 V for 1 mM Hg, 0.1 mM Pb and 1 mM Cd, respectively in 0.1 M KCl solution using the RH-SiC-modified electrode. Solution pH value of 7, deposition potential of - 1.5 V, deposition time of 120 s and standing time of 60 s are the optimal operating parameters. The detection range and limit of detection are 0.63 nM and 0.01-1000 µM, 0.45 nM and 0.001-100 µM, 0.87 nM and 0.01-1000 µM for simultaneously detection of Hg, Pb and Cd. The RH-SiC-modified electrode exhibits good selectivity, reproducibility, stability and practical applicability for efficiently detecting Hg, Pb and Cd.
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8. Characterization and Immobilization of a High Activity Lysine Decarboxylase from Serratia Proteamaculans NJ303 for Cadaverine Production.
PMID:日期:2026-10-01Cadaverine, an essential precursor for bio-based nylon PA5X production, has emerged as a highly valuable compound in the engineered plastics and synthetic fibers industry. Current industrial biomanufacturing of cadaverine depends critically on the enzymatic activity of L-lysine decarboxylase (LDC). In this investigation, we systematically evaluated ten LDC variants originating from six distinct bacterial species, all cloned and expressed in Escherichia coli BL21(DE3). Comprehensive characterization identified SpLDC from Serratia proteamaculans as the most promising candidate, demonstrating exceptional catalytic activity under alkaline conditions. Enzymatic analysis showed that SpLDC established optimal activity at pH 6.5 and 52 °C, with remarkable stability maintained between pH 5.5-8.0 and temperatures of 37-52 °C during prolonged 12-hour incubations. Kinetic studies revealed favorable catalytic parameters (K = 11.5 mM, V = 1000 U/mg), indicating efficient substrate conversion. To enhance industrial applicability, we developed an immobilized enzyme system by genetically fusing SpLDC with a chitin-binding domain (ChBD) and subsequently immobilizing it on chitin supports. This engineered biocatalyst demonstrated superior performance compared to whole-cell systems, achieving a 3.4-fold enhancement in cadaverine production efficiency. The immobilized preparation exhibited excellent operational stability, retaining over 50% of its initial activity through ten repeated reaction cycles. This work establishes an efficient, stable, and reusable enzymatic platform for cadaverine biosynthesis, offering significant potential for sustainable nylon PA5X production at industrial scales.
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9. Tandem Repeat Gene Strategy for High-Yield Production and Functional Evaluation of Bioactive Wheat Oligopeptides.
PMID:日期:2026-10-01Bioactive wheat peptides are promising functional ingredients, yet their industrial production remains constrained by low yields, product heterogeneity, and poor scalability associated with conventional enzymatic hydrolysis and chemical synthesis. Here, we report a tandem-repeat gene strategy for the scalable biosynthesis of the representative wheat peptide YDW (Tyr-Asp-Trp-Pro-Gly-Gly-Arg-Asn). By integrating codon optimization with engineered pepsin-cleavable linkers, high-molecular-weight precursor proteins were efficiently expressed in Escherichia coli and subsequently converted into homogeneous target peptides through site-specific enzymatic processing. Systematic evaluation of repeat architectures identified the 24-repeat construct as optimal, reflecting a balance between gene length, mRNA stability, and translational burden. This system achieved peptide yields of up to 5 g/L in a 700 L fermentation process, demonstrating strong scalability. The resulting peptides exhibited high purity and correct molecular weight, as confirmed by HPLC and LC-MS/MS analyses. Functional assays further showed that YDW significantly enhanced cellular collagen I expression by over 240% relative to controls, supporting its potential in anti-photoaging and tissue repair applications. This work establishes a generalizable and scalable platform for the biomanufacturing of cereal-derived bioactive peptides, providing a practical alternative to conventional methods and enabling future studies on peptide structure-function relationships and industrial applications.
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10. Blue Carbon Dots Modified Cu-MOF: Excellent Peroxidase-Like Activity and Ratiometric Fluorescence Sensing to L-Cys.
10. 蓝碳点修饰的Cu-MOF：优异的过氧化物酶样活性和对L-Cys的比率荧光传感PMID:日期:2026-10-01Blue carbon dots (B-CDs) were prepared via a hydrothermal method using citric acid and urea as precursors, and subsequently employed to construct a carbon dots/MOF-based composite (B-CDs@Cu-MOF) through an in situ one-pot approach. The B-CDs were successfully incorporated into the pores and onto the surfaces of the Cu-MOF, without significantly altering its octahedral crystalline structure, but with a slight decrease in surface area. Due to the reduction nature of the B-CDs, partial reduction of Cu(II) occurred in the synthetic process, resulting in the coexistence of Cu(II) and Cu(I) within the B-CDs@Cu-MOF. The peroxidase (POD)-like catalytic activity of B-CDs@Cu-MOF was investigated systematically using the oxidation of o-phenylenediamine (OPD) in the presence of HO. The B-CDs@Cu-MOF exhibits distinctly improved POD-like activity, compared to the original Cu-MOF and other previously reported Cu-based POD mimics. The catalytic kinetics parameters including Michaelis constant (K) and maximum initial velocity (V) were determined. In addition, these B-CDs@Cu-MOF show fluorescence emission at 450 nm, attributed to the B-CDs. By integrating it with the fluorescence emission at 564 nm from 2,3-diaminophenol (DAP), a product of OPD oxidation, a ratiometric sensing platform was developed. This platform, composed of B-CDs@Cu-MOF, OPD and HO, was used to detect L-cysteine (L-Cys). In this system, L-Cys competitively consumes reactive oxygen species (ROS) and inhibit DAP formation. Based on that, a ratiometric fluorescence sensor for L-Cys detection was constructed, exhibiting a linear response range from 10 µmol·L to 100 µmol·L, and a detection limit of 1.77 µmol·L. This work well integrated the enzyme-like catalysis of Cu-MOF and the fluorescence properties of CDs, that provides an excellent ratiometric sensing platform toward target molecules.