APPLIED MICROBIOLOGY AND BIOTECHNOLOGY应用微生物学与生物技术
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY(英文缩写 APPL MICROBIOL BIOT),ISSN 0175-7598,eISSN 1432-0614,中文译名:应用微生物学与生物技术 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.560 | Q1 |
| 2022 | 5.000 | Q1 |
| 2023 | 3.900 | Q2 |
| 2024 | 4.300 | Q1 |
| 2025 | 4.700 | Q2 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 最新收录文献
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1. Alluvial filtration as a scalable and safe single-use technology for the harvest of viral vectors.
1. 冲积过滤作为一种可扩展且安全的单次使用技术用于病毒载体的收获PMID:日期:2026-10-01Recent advances in gene therapy have increased the demand for large-scale manufacturing processes. The production of recombinant adeno-associated viral (rAAV) vectors, the predominant vectors used for gene delivery, requires efficient scalable processes for production and purification which have to comply with high safety standards. To enable operation in a fully closed system, the exclusive use of single-use materials and equipment is indispensable. The present study reports on the use of alluvial filtration for the clarification of lysed rAAV vector production culture from various production scales. In small-scale experiments (1-3 L feed), the in-house developed method was superior to the use of a synthetic depth filter regarding clarification performance for capacity, turbidity reduction, product recovery, and impurity reduction. Scale-up calculations for 500 L harvest revealed that alluvial filtration could provide the required filter area and capacity within a fully closed system, whereas the use of the depth filter could not. Real-life scale-up data for processing 10 L, 100 L, and 500 L with different rAAV vector variants confirmed scalability of the alluvial filtration method and yielded excellent results regarding its clarification performance. Moreover, the full filter capacity and limits of the system were not reached, indicating the robustness of the process to varying feedstock compositions. The safety of the established alluvial filtration train was supported by implementing enhanced monitoring and control to mitigate the risk of failure and leakages. Overall, the harvest of rAAV vectors by alluvial filtration proved to be superior to established depth filters within a biopharmaceutical production process. KEY POINTS: • Large-scale viral vector production (> 100 L) to meet increasing gene therapy demand • Alluvial filtration outperforms depth filters in efficient harvest clarification • Alluvial filtration offers capacity for closed-system scale-up to 500 L production.
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2. What we (still don't) know about wood-degrading fungi: an overview of threats and utilization potentials.
2. 我们对木质降解真菌（仍然）未知的方面：威胁与利用潜力概述PMID:日期:2026-09-28Wood, being one of the most abundant materials on Earth, is a hierarchically structured lignocellulosic biocomposite that has evolved to resist decomposition by other organisms. While lignin is thought to have evolved in the Devonian (~ 400-390 Ma), leading to the deposition of vast coal reserves in the Carboniferous (~ 360-299 Ma), a diverse group of fungi evolved around that time to precisely break through this barrier and use it as carbon source. The emerging "wood-degrading fungi" since then play a fundamental role in global nutrient cycling, carbon flow, and material turnover. The significance of wood-degrading fungi lies both in their applicability thanks to their enzyme systems, transporters, and metabolites, and in their being threats to forests, wood products, and health. This duality is similar, albeit maybe less known to the public, than the one in the food industry, where fungi have both useful applications (e.g., in food coloring, food fermentation, and as protein sources) and risks (e.g., through mycotoxins). Moreover, studies on fungal colonization and substrate attack in connection with fungal degradation in the built environment have expanded our mechanistic understanding of their natural behavior. In the present mini-review, we provide an overview of the mechanisms by which fungi degrade wood and discuss both the threats to living trees and wood products associated with wood-degrading fungi as well as their potential for added value in the bioeconomy. We furthermore aim to highlight important macroscopic and microscopic aspects to provide an integrative view of the mechanisms involved, including data retrieved by genomic and literature meta-analyses. This mini-review integrates insights from the literature with exploratory analyses of diverse databases to provide a holistic view of wood-degrading fungi, supporting future perspectives and a multidimensional understanding of these organisms.
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3. Graphene Oxide and Methylglyoxal: a combined strategy against chronic wound pathogens.
3. 氧化石墨烯与甲基乙二醛：对抗慢性伤口病原体的联合策略PMID:日期:2026-09-24Chronic wound infections are difficult to treat due to the polymicrobial biofilms that delay wound healing increasing antimicrobial tolerance and limiting patient compliance. To address this challenge, we developed a non-antibiotic combination of Graphene Oxide (GO) and Methylglyoxal (MGO) (patent N. 102022000024408-Composizione per il trattamento delle infezioni a carico delle lesioni cutanee) against clinically relevant chronic wound pathogens. For the tests, clinical antimicrobial resistant Staphylococcus aureus and Pseudomonas aeruginosa were used. MGO MIC was determined and the best GO + MGO combination, evaluated by using checkboard test, was tested in terms of: i) fluid membrane changes; ii) S. aureus and P. aeruginosa CFUs/mg reduction in Lubbock Chronic Wound Biofilm-LCWB, a recognized polymicrobial in vitro chronic wound biofilm model; iii) P. aeruginosa motility. MGO MIC values ranged from 32 to 128 mg/l. Notably, synergistic interactions between GO and MGO were observed exclusively against S. aureus, whereas the combinations showed additive effects against P. aeruginosa. The best GO + MGO (6.25 + 64 mg/l) combination increased bacterial membrane fluidity, inhibited informing and mature LCWBs by 60-80% in terms of CFU/mg and P. aeruginosa twitching motility. GO + MGO combination exhibited an additive antimicrobial/antibiofilm activity with a multi-target action. Overall, the GO + MGO combination, at recognized non-toxic concentrations, is a valid and innovative non-antibiotic solution for wound management affecting the polymicrobial chronic wound biofilms and P. aeruginosa motility.
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4. Fermentation-based oxalic acid production aimed at sustainable critical metal recovery from electronic waste.
4. 基于发酵法的草酸生产，旨在从电子废弃物中可持续地回收关键金属PMID:日期:2026-09-23This study systematically investigates the biogenic production of oxalic acid (OA, HCO) via submerged fermentation to establish a sustainable reagent for critical metal recovery applications. Preliminary shake-flask experiments evaluated Aspergillus niger strains (ATCC 1015 and CECT 2807), optimal carbon sources, and the influence of pH adjustment. Despite inherent biological variance across replicates, A. niger ATCC 1015 demonstrated a superior production trajectory, yielding 71.0 ± 27.7 mM OA in 5 days. Intermittent pH adjustment above 4.0 significantly enhanced secretion to 90.4 ± 5.8 mM, while glucose was identified as the optimal carbon source (Y 0.4 g/g). Building upon these preliminary findings, process intensification was conducted in a 10 L stirred-tank bioreactor. Transitioning to a fed-batch strategy with pulsed feeding and continuous pH control effectively contributed to substantially higher OA titers, achieving a peak OA titer of 260.1 ± 4.8 mM over 14 days. Co-production of gluconic acid and acidogenesis inhibition posed a challenge in attaining higher product yields. Collectively, these findings establish a robust, scalable bioprocess for sustainable OA generation, directly supporting its emerging application as a highly selective leaching agent for critical metal recovery from electronic waste streams. KEY POINTS: A.niger strain ATCC1015 was pinpointed as a suitable strain for biogenic OA production Fed-batch with pulsed feeding maximizes OA titer in scale-up. Gluconic acid and phosphate excess hinder selective OA production.
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5. Correction: Efficient biotechnological production of N-(Hydroxycinnamoyl) tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation.
5. 更正：利用植物源酶在带原位产物沉淀的台式生物反应器中高效生物技术生产N-(羟基肉桂酰基)酪胺PMID:日期:2026-09-19该文献暂无摘要。
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6. Disulfiram reverses fluconazole-resistance mediated by Cdr1 in Candida albicans and interferes with biofilm formation.
6. 双硫仑逆转白色念珠菌中由Cdr1介导的氟康唑耐药性并干扰生物膜形成PMID:日期:2026-09-12The incidence of Candida albicans infections has risen markedly, particularly among immunocompromised individuals and cancer patients undergoing chemotherapy. Fluconazole is commonly used as a first-line treatment for C. albicans infections; however, increasing clinical resistance poses a significant therapeutic challenge. As a result, developing new strategies to enhance the management of C. albicans infections has become critical. This study aims to assess the prevalence of azole's resistance among clinical C. albicans isolates in Egypt and to evaluate disulfiram/fluconazole combination against fluconazole resistant isolates. A total of 64 C. albicans clinical isolates were tested for fluconazole susceptibility using disc diffusion method and broth microdilution method (alone and in combination with disulfiram). The combination of fluconazole and disulfiram was further assessed using checkerboard method. The expression levels of resistance determinants were analyzed using RT-PCR. Molecular docking was used for further analysis. Sixteen isolates (25%) were resistant to fluconazole. Combination with subinhibitory concentration of disulfiram reduced the minimum inhibitory concentration (MIC) of fluconazole in resistant isolates (2-128 fold). Checkerboard assay revealed that fluconazole/disulfiram combination was mostly synergistic. In fluconazole-resistant isolates, Cdr1 was overexpressed, whereas disulfiram (at subinhibitory concentration) has triggered its downregulation. Docking studies showed that disulfiram may compete with ATP for binding and may disrupt fluconazole's interaction with Cdr1. Additionally, disulfiram exhibited a potent anti-biofilm activity against the tested C. albicans isolates. Fluconazole/disulfiram combination appears to be a promising approach for overcoming fluconazole resistance in C. albicans. Further studies are needed to validate this finding and explore its clinical potential. KEY POINTS: • Fluconazole/disulfiram combination is a promising approach to overcome fluconazole resistance in C. albicans • Disulfiram triggered downregulation of Cdr1 expression in fluconazole-resistant C. albicans. • Disulfiram competes with ATP for Cdr1 binding, disrupting fluconazole-Cdr1 interaction.
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7. An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species.
PMID:日期:2026-09-11Advances in molecular biology tools are essential for streamlining and accelerating genetic engineering of cells across industrial and academic applications. While CRISPR-Cas improves genome editing efficiency, current systems have limitations and are often host specific, which restricts their versatility. This study describes a versatile CRISPR-Cas9 system for genome editing in industrially relevant Bacillus species. By adapting the well-established pJOE8999 vector-based CRISPR-Cas9 genome editing system, we constructed an inducer-independent, broad-host-range genome editing system. It maintains the benefits of low toxicity to the target cell and the cloning host as well as the ease to use of a single-plasmid CRISPR-Cas9 system. We utilized the constitutive Sigma70-type promoter from the conserved veg gene of Bacillus, to develop and test the suitability of promoter variants of different strengths for Cas9 expression. Successful gene deletions in three different Bacillus species demonstrated the versatility of the modified system for this industrially important genus. This was further confirmed by the integration of a reporter gene fusion and the introduction of a single point mutation in the genome of Bacillus licheniformis. This one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time. KEY POINTS: • Editing and screening of promoter variants for balanced Cas9 expression in Bacillus. • Development of a versatile inducer-independent, single-plasmid CRISPR-Cas-based system. • Verification of the modified CRISPR-based system for genome editing in different Bacilli.
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8. Synthetic consortia towards photosynthetically derived acetate for heterotrophic production of 1-butanol.
8. 利用合成菌群实现光合来源乙酸盐用于异养生产1-丁醇PMID:日期:2026-09-11Synthetic consortia is an emerging field in biotechnology, offering advantages such as division of labor between the individual members and reduced risk of contamination. Consortia combining phototrophic and heterotrophic bacteria are attractive, as phototrophs can utilize CO₂ and light energy to sustain growth. Several studies have explored such systems where sucrose is most commonly used as the provided carbon source. An alternative is acetate, a smaller two-carbon molecule produced as a by-product by many microorganisms, including the model cyanobacterium Synechocystis PCC 6803 (thereafter Synechocystis). Although acetate production during phototrophic growth is typically low, previous studies have demonstrated that metabolic engineering-specifically the introduction of phosphoketolase (PK) and overexpression of phosphotransacetylase (Pta)-enabled the development of a high-producing strain (WT_PKPa_RBS_BsPta_Δacs) capable of secreting significant levels of acetate into the medium (Roussou and Lindblad 2026). In this study, Escherichia coli and Pseudomonas taiwanensis were engineered to produce 1-butanol, an industrially relevant bulk chemical, and cultivated using acetate as the sole carbon source. These strains were then individually co-cultivated with the previously engineered Synechocystis strain, WT_PKPa_RBS_BsPta_Δacs, forming two distinct consortia that were maintained for 42 days. Growth dynamics were successfully monitored, and acetate concentrations in the consortia were lower than in a corresponding axenic Synechocystis culture. At the same time, 1-butanol production was detected in the two co-cultures, demonstrating the feasibility of coupling photosynthetically derived acetate to heterotrophic production of a value-added bulk chemical. KEY POINTS: • Photosynthetic/heterotrophic synthetic consortia for 1-butanol production. • Engineered Synechocystis PCC 6803 cells produce acetate from CO. • Modified Escherichia coli and Pseudomonas taiwanensis cells grow on acetate and produce 1-butanol.
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9. Identification and characterization of TrxA: a novel bacterial thioredoxin-metallothionein chimera.
9. TrxA的鉴定与表征：一种新型细菌硫氧还蛋白-金属硫蛋白嵌合体PMID:日期:2026-08-29Heavy metals (HMs) are naturally occurring elements which can be essential, such as zinc, copper, and iron, or non-essential, including cadmium, mercury, and lead. While essential metals serve as cofactors in critical enzymatic processes, elevated concentrations of both essential and non-essential HMs pose severe toxicity risks, primarily through oxidative stress, disruption of metal homeostasis, and biomolecular damage. Microorganisms have evolved diverse mechanisms to cope with metal-induced stress, including metal sequestration, enzymatic transformation, efflux systems, and surface immobilization. Among these, metallothioneins (Mts) are small, cysteine-rich proteins capable of high-affinity metal binding, contributing to cellular detoxification. Although Mts have been extensively studied in eukaryotes, knowledge of bacterial Mts remains limited, with characterized examples largely confined to cyanobacteria and a few other bacterial species. In this study, we identified a novel hybrid protein, TrxA, from Runella aurantiaca, containing a thioredoxin (Trx) domain fused to a Mt domain. The presence of the Trx domain may confer improved stability and solubility, supporting potential recombinant applications. In fact, the recombinant protein, named TrxMt, was heterologously expressed in Escherichia coli, displaying both disulfide-reducing activity and heavy metal-binding capability. Notably, TrxMt expression enhanced bacterial tolerance to multiple HMs, demonstrating its functional relevance in vivo. These findings expand the understanding of bacterial Mt diversity and suggest that TrxMt is a promising candidate for the bioremediation of heavy metal-contaminated environments, combining metal detoxification with favorable biochemical properties for industrial and environmental applications. KEY POINTS: • Identification of TrxA, a novel hybrid thioredoxin-metallothionein in R. aurantiaca • Recombinant protein TrxMt shows reductase activity and binds HMs • Overexpression of TrxMt enhances tolerance to different HMs in E. coli.
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10. Construction of a novel reporter strain indicating inhibition of cell wall biosynthesis.
10. 构建一种指示细胞壁生物合成受抑制的新型报告菌株PMID:日期:2026-08-28The WalRK two-component system monitors cell wall biosynthesis, and its activity decreases when biosynthesis is inhibited. As WalRK is essential for many Gram-positive bacteria, including Staphylococcus aureus and Bacillus subtilis, directly inhibiting WalK would disrupt cell wall biosynthesis and result in cell death. This study presents the construction of new reporter strains with two WalRK-dependent promoters: PiseA and PssaA. These reporter strains enable indirect in vivo measurement of WalRK activity based on luminescence. We evaluated these reporters in combination with the established PliaI reporter system, which is regulated by LiaRS, in order to improve the identification of cell wall inhibitors during the screening of natural products. The WalRK-dependent PiseA reporter yielded specific responses to almost all known cell wall biosynthesis inhibitors, including β-lactams, tunicamycin, glycopeptides, daptomycin, and fosfomycin, with no discernible response to other antibiotic classes. Furthermore, combining the PliaI reporter system with the PiseA reporter enhanced the range of potential cell wall inhibitors that could be detected. Deleting the gene of the eukaryotic-like Ser/Thr kinase PrkC increased the PiseA signal obtained with certain antibiotics, as well as after a longer incubation period. Additionally, deletion of prkC lowered PliaI expression in the presence of lipid II-targeting antibiotics. Screening natural products with an unknown mode of action identified several compounds that elicited vancomycin-like patterns with the reporter systems, indicating potential cell wall inhibitory activity. Overall, combining WalRK and LiaRS reporters enables a rapid, complementary evaluation of cell wall stress responses, providing a powerful method for discovering new cell wall-targeting antibiotics. KEY POINTS: • The PiseA/PssaA reporters enable continuous in vivo observation of WalRK activity • Cell envelope stress responses are differentially sensed by WalRK and LiaRS • The PiseA reporter strain detects nearly all cell wall biosynthesis inhibitors.