PLANT SCIENCE植物科学
PLANT SCIENCE(英文缩写 PLANT SCI),ISSN 0168-9452,eISSN 1873-2259,中文译名:植物科学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.363 | Q1 |
| 2022 | 5.200 | Q1 |
| 2023 | 4.200 | Q1 |
| 2024 | 4.100 | Q1 |
| 2025 | 4.600 | Q1 |
PLANT SCIENCE 最新收录文献
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1. {"_":"AHL13, AHL19 and AHL20 Genes Differentially Regulate Cell Wall-Associated Defense Responses under Fumonisin B-Induced Stress in Arabidopsis thaliana.","sub":["1"]}
PMID:日期:2026-10-03Fumonisin B (FB), a mycotoxin produced by Fusarium species, induces oxidative stress and cell wall-associated defense responses in plants. However, the regulatory factors that determine how different components of cell wall defense are coordinated under toxin-induced stress remain poorly understood. AT-hook motif nuclear-localized (AHL) proteins are chromatin-associated transcriptional regulators, but their roles in FB-induced cell wall remodeling have not been directly examined. In this study, we investigated the distinct roles of AHL13, AHL19, and AHL20 genes in FB-induced defense responses in Arabidopsis thaliana. Wild-type Col-0 and ahl13, ahl19, and ahl20 loss-of-function mutants were treated with 10µM FB for 4 days, and growth, oxidative stress markers, defense-related enzyme activities, and cell wall associated gene expression were analyzed. The three mutants displayed genotype-specific responses rather than a uniform defense pattern. In ahl13 mutant, weakening of the peroxidase-associated responses was accompanied by activation of biosynthetic and remodeling-related components. In ahl19 mutant, a biosynthesis-oriented wall defense program became more prominent. In ahl20 mutant, oxidative damage remained limited, while selected antioxidant and defense mechanisms were activated. These findings indicate that AHL13, AHL19, and AHL20 genes do not simply enhance or suppress FB-induced cell wall defense. Instead, they appear to act as regulatory factors that may differentially influence ROS production/detoxification, peroxidase activity, phenylpropanoid input, callose-related responses, cellulose biosynthesis, and wall remodeling pathways.
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2. Genome-Wide Characterization of the DUF966 Gene Family in Rice and Functional Analysis of OsDSR6 via Overexpression.
PMID:日期:2026-10-03We previously identified seven members of the domain of unknown function 966 (DUF966) family in rice and showed that OsDSR2 and OsDSR3 regulate tolerance to drought, salinity and alkali stresses. However, bioinformatic characterization of this family remains limited, and the biological functions of the remaining members are largely unexplored. In this study, we performed a systematic bioinformatic analysis of the structure and potential function of the rice DUF966 family and focused on the unidentified member OsDSR6 in salinity tolerance. Phylogenetic and collinearity analyses showed that DUF966 genes are more evolutionarily conserved in monocot species. Promoter analysis indicated that family members commonly contain light-responsive elements, abscisic acid-responsive elements (ABREs) and methyl jasmonate (MeJA)-responsive elements. Transcriptional regulatory prediction identified 25 putative transcription factors and 91 microRNA-target interactions associated with this family. Among them, NIGT1 (LOC_Os02g22020) and LOC_Os05g37730 were predicted to bind the promoter regions of OsDSR3 and OsDSR7, respectively, OsDSR6 was predicted to be regulated by osa-miR399a/b/c. OsDSR6 exhibited high expression levels in rice leaves and leaf sheaths. Its expression was rapidly downregulated by salt and drought stress but upregulated by abscisic acid (ABA) treatment. The OsDSR6-encoded protein is localized to the plasma membrane. Functional analysis showed that overexpression of OsDSR6 increases rice sensitivity to salt stress, and the transcript levels of stress-responsive genes, including Lea3, Rab16c, P5CS and OsNCED4, were decreased in OsDSR6-overexpressing plants. Collectively, these findings extend current understanding of the rice DUF966 gene family and identify OsDSR6 as a potential target for molecular breeding of salt-tolerant rice.
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3. Exogenous Melatonin Confers Thermotolerance in Water Spinach by Modulating Redox Metabolism and the Ascorbate-Glutathione Cycle.
PMID:日期:2026-10-02Aims High-temperature (HT) stress significantly impairs plant growth by impairing antioxidant enzyme activities and the photosynthetic apparatus. Melatonin (ME) is a novel plant hormone that plays diverse roles in regulating tolerance to abiotic stresses. This study examined the role and potential of ME in mitigating the negative impacts of heat stress on water spinach plants. Methods Different concentrations of ME (50, 100, 150, and 200µM) were foliar-sprayed for 12 days (every 3 days). After ME pretreatment, water spinach plants were exposed to heat stress (40 °C) for 96hours. Results HT stress dramatically decreased biomass production by hindering pigment concentration, lowering gas exchange parameters, impairing root architecture, and altering antioxidant enzyme activities. In contrast, foliar application of ME (100µM) significantly improved biomass production, photosynthetic performance, and root architecture. In addition, ME (50-200µM) effectively reduced the accumulation of HO and O, restored cellular membrane integrity by reducing MDA accumulation, and regulated the ascorbate-glutathione cycle. Furthermore, foliar application of ME considerably increased secondary metabolite concentrations (anthocyanin, flavonoids, and phenols), and these secondary metabolites strongly controlled ROS overproduction. Conclusions Our study found that ME (100µM) may effectively mitigate heat-induced stress in water spinach by enhancing the photosynthetic machinery, regulating redox homeostasis, reducing cellular membrane damage, and modulating antioxidant defense and the AsA-GSH cycle.
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4. Role of the CONSTANS protein in regulating flowering time in Thlaspi arvense L.
PMID:日期:2026-10-01The floral transition, which denotes the shift from vegetative to reproductive growth, is a fundamental process in plant development that critically influences both plant reproductive success and crop yield. Photoperiod, defined as the perception of day length, acts as a crucial environmental cue regulating floral transition in numerous plant species. Thlaspi arvense L., a promising oilseed crop within the Brassicaceae family, demonstrates photoperiod-insensitive flowering in its Kunming ecotype. To elucidate the underlying mechanisms, homologs of the Arabidopsis thaliana CONSTANS (CO) and FLOWERING LOCUS T (FT) genes were identified in Thlaspi arvense L., and designated TaCO-like1 (TaCO1) and TaFT-like1 (TaFT1). Both genes exhibit core conserved characteristics akin to their Arabidopsis counterparts. The TaCO1 protein localizes to the nucleus with predominant leaf expression, whereas TaFT1 distributes in both the nucleus and cytoplasm. Moreover, both genes show rhythmic expression. Functionally, the heterologous overexpression of either TaCO1 or TaFT1 accelerates flowering in Arabidopsis, and TaCO1 activates TaFT1 transcription. Importantly, TaCO1 significantly diminished the disparity in flowering time between long-day and short-day conditions in Arabidopsis co-9 mutants, suggesting that it may have a more important flowering-promoting function under short-day conditions. This functional diversification of TaCO1 provides a molecular basis for the unique flowering mechanism observed in the Kunming ecotype and presents a potential target for the genetic manipulation of flowering time in this oilseed crop.
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5. A LysM receptor-like kinase TaLYK5 positively regulates wheat resistance to leaf rust.
PMID:日期:2026-10-01Wheat leaf rust, caused by the biotrophic fungus Puccinia triticina (Pt), is one of the most destructive diseases threatening global wheat production. Lysin motif (LysM)-containing receptors play central roles in plant recognition of fungal chitin and activation of pattern-triggered immunity (PTI), but the function of the LysM receptor-like kinase TaLYK5 in wheat resistance to leaf rust remains largely unknown. Here, we show that TaLYK5 lacks conserved kinase motifs essential for catalytic activity and is predicted to be a pseudokinase, suggesting that it may function through a co-receptor to transmit chitin signals. The transcript levels of TaLYK5 were rapidly and persistently upregulated upon Pt infection and chitin treatment in wheat. Transient expression of TaLYK5-6A in Nicotiana benthamiana significantly enhanced chitin-induced callose deposition. Transient silencing of TaLYK5 in wheat compromised resistance to Pt, with reduced H₂O₂ accumulation and increased fungal colonization. Importantly, stable overexpression of TaLYK5-6A in transgenic wheat lines conferred enhanced resistance to Pt, as shown by significantly fewer uredia and lower fungal biomass. Furthermore, overexpression of TaLYK5-6A enhanced chitin-induced callose deposition and expression of PTI marker genes. Collectively, these results demonstrate that TaLYK5 positively regulates wheat resistance to leaf rust, and this regulation is associated with chitin-triggered PTI, highlighting its potential as a candidate gene for improving leaf rust resistance in wheat.
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6. TaWRKY24 positively regulates wheat resistance to Puccinia striiformis f. sp. tritici via modulation of defense signaling and reactive oxygen species accumulation.
6. TaWRKY24通过调节防御信号和活性氧积累正调控小麦对条锈菌的抗性PMID:日期:2026-09-29Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), remains a major threat to global wheat production, which necessitates a better understanding of the molecular mechanisms underlying resistance. WRKY transcription factors are pivotal regulators of plant immunity; however, their specific functions in the wheat-Pst pathosystem remain poorly characterized. In this study, we identified and functionally characterized a WRKY transcription factor gene, TaWRKY24, in wheat. Expression profiling revealed that TaWRKY24 was continuously induced upon Pst inoculation. Subcellular localization assays demonstrated that the TaWRKY24 protein is predominantly localized to the nucleus. Functional analyses indicated that transient overexpression of TaWRKY24 significantly enhanced wheat resistance to Pst, whereas virus-induced gene silencing (VIGS) compromised wheat resistance to Pst. Histological observations revealed that TaWRKY24 promotes reactive oxygen species (ROS) accumulation at early infection sites and restricts fungal hyphal expansion. To further explore the underlying mechanism, combined transcriptomic and qRT-PCR analyses showed that silencing TaWRKY24 resulted in significant downregulation of key genes involved in MAPK signaling (MKK4/5, WRKY33), glutathione metabolism (GSS), and phenylpropanoid biosynthesis (PAL). In addition, yeast one-hybrid and dual-luciferase reporter assays demonstrated that TaWRKY24 directly binds to and activates the promoters of PAL- and MAPK-related genes. Collectively, our findings suggest that TaWRKY24 positively regulates wheat resistance to Pst, likely through the modulation of MAPK-mediated immune signaling, ROS homeostasis, and defense-related metabolic pathways. This study provides novel insights into the regulatory functions of WRKY transcription factors in wheat innate immunity.
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7. Functional validation of EXLB1 in leaf and stomatal development in tobacco.
PMID:日期:2026-09-29Leaf morphological traits are the key determinants of tobacco yield and stress responses, and cell wall synthesis and metabolism play an important role in leaf development. However, the underlying regulatory mechanism of cell wall on leaf morphology remains largely unknown in tobacco. Here, we compared two pairs of tobacco cultivars (DMC vs. DX3 and DMC vs. WM3) with distinct leaf traits, and identified a cell wall-related expansin gene, NtEXLB1, based on transcriptome data analysis. The homolog NbEXLB1 from N. benthamiana is highly expressed in the mature leaf, and the NbEXLB1 protein is localized to the cell wall. NbEXLB1 gene overexpression promoted leaf expansion with larger abaxial pavement cells, decreased stomatal density and aperture in N. benthamiana. NbEXLB1 CRISPR mutant plants exhibited smaller pavement cells and more stomata, while the leaf area did not show any significant difference. Furthermore, overexpression of NbEXLB1 remarkably enhanced drought tolerance and resistance to Rhizoctonia solani infection. These findings provide mechanistic insights into how cell wall regulation coordinates leaf development, and highlight NbEXLB1 as a promising genetic resource for improving leaf productivity and stress resilience in tobacco breeding.
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9. A gain-of-function mutation in SlUVR8 reveals critical roles of UV-B signaling in tomato development and stress tolerance.
PMID:日期:2026-09-26Studying the functions of the UV-B photoreceptor UVR8 in large, indeterminate-growth tomato plants is challenging due to the difficulty of applying uniform UV-B irradiation. To overcome this limitation, we generated a constitutively active photoreceptor variant, SlUVR8, by substituting tryptophan 289 with alanine. This variant enables functional investigation of SlUVR8 in tomato without the need for UV-B treatment. Size exclusion chromatography coupled with western blot analysis revealed that the W289A substitution destabilizes SlUVR8 homodimers and alters protein conformation. In transgenic tomato lines expressing SlUVR8, the variant enhanced photomorphogenesis and suppressed thermomorphogenesis at the seedling stage in the absence of UV-B, and also conferred substantial tolerance to UV-B stress without prior acclimation. Notably, SlUVR8 failed to activate UV-B-induced early transcriptional responses in seedlings. In mature plants, SlUVR8 significantly increased chlorophyll accumulation in fruits by regulating key genes involved in chlorophyll biosynthesis. Collectively, our results demonstrate that SlUVR8 represents a constitutively active UVR8 photoreceptor for long-term UV-B responses, allowing the dissection of UV-B signaling pathways independently of UV-B exposure. This study establishes intrinsic UV-B signaling as a critical regulator of seedling establishment, fruit development, and stress adaptation in tomato. Our findings provide a mechanistic basis for harnessing UV-B signaling to improve horticultural crop performance, and position SlUVR8 as a valuable genetic tool for both fundamental UV-B research and practical crop improvement.
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10. Combined transcriptomic and metabolomic analysis reveals molecular mechanisms governing the effect of foliage-sprayed calcium chloride on tomato fruit quality.
PMID:日期:2026-09-25Calcium fertilizers are widely used to enhance tomato fruit quality, however, the molecular mechanisms underlying these effects remain not fully elucidated. In this study, 'Micro-Tom' tomato plants were subjected to foliar sprays of calcium chloride at different concentrations, and 0.05 mol·L⁻¹ was identified as the optimal concentration. Transcriptomic profiling, untargeted metabolomics, and RT-qPCR validation were integrated to characterize the molecular responses to calcium supplementation. Foliar application of 0.05 mol·L⁻¹ calcium chloride significantly increased fruit firmness, vitamin C content, lycopene content, and the sugar-acid ratio. Untargeted metabolomics identified 429 differentially accumulated metabolites, including 281 upregulated and 148 downregulated metabolites, with lipids and phenylpropanoids representing the most enriched classes. Transcriptomic analysis identified 2514 differentially expressed genes, which were predominantly enriched in the phenylpropanoid biosynthesis pathway. Several key genes in this pathway, including SlCCoA-OMT, SlPER42, SlPER43, and Sl1-CysPrx, were significantly upregulated following calcium treatment. The increased accumulation of two lignin precursors, ferulic acid and sinapic acid, together with the enhanced expression of their pathway genes, was associated with greater fruit firmness, suggesting that increased precursor availability may promote cell wall reinforcement. These results identify candidate genes and metabolic pathways associated with calcium-mediated improvements in tomato fruit quality and provide preliminary molecular insights into calcium fertilization and the breeding of high-quality tomato cultivars.