APPLIED AND ENVIRONMENTAL MICROBIOLOGY应用与环境微生物学
APPLIED AND ENVIRONMENTAL MICROBIOLOGY(英文缩写 APPL ENVIRON MICROB),ISSN 0099-2240,eISSN 1098-5336,中文译名:应用与环境微生物学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.005 | Q2 |
| 2022 | 4.400 | Q2 |
| 2023 | 3.900 | Q2 |
| 2024 | 3.700 | Q2 |
| 2025 | 4.200 | Q2 |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 最新收录文献
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1. The gut within: reframing the coral gastrovascular cavity as a discrete anaerobic microbial ecosystem.
1. 肠道之内：将珊瑚胃血管腔重新定义为独立的厌氧微生物生态系统PMID:日期:2026-10-05Coral holobionts are typically studied at colony or fragment scale, erasing the spatial context of the microenvironments which microbial life occupies. Recent microsensor work (Q. Zhang, E. Bollati, and M. Kühl, Appl Environ Microbiol 92:e02441-25, 2026, https://doi.org/10.1128/aem.02441-25) shows the coral gastrovascular cavity (GVC) sustains active anaerobic metabolism, reflecting patterns of symbiotic state and heterotrophic feeding. These findings recast the GVC as a discrete, gut-like ecosystem with its own redox regime, substrate supply, and bacterial community, calling for spatially explicit approaches in coral research to resolve metabolism at microscopic scales.
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2. Impacts of temperature on hydrogenotrophic denitrifying aquifer microbiota.
2. 温度对氢自养反硝化含水层微生物群落的影响PMID:日期:2026-10-05Groundwater is a globally relevant drinking water resource, yet nitrate pollution from agriculture increasingly threatens its quality, especially in shallow, oxygen-rich aquifers where natural attenuation is limited. This study examined whether gaseous hydrogen can stimulate autochthonous chemolithoautotrophic denitrification in nitrate-polluted, gravel aquifer sediments, and whether this process remains effective across temperatures relevant for laboratory and field conditions. We hypothesized that both microbial community composition and the kinetics of nitrate reduction will change under distinct temperature regimes. Microcosm incubations of sediments from a nitrate-polluted aquifer successfully enriched native hydrogenotrophic denitrifiers. Denitrification activity was quantified at 12°C, 15°C, 20°C, and 25°C, with maximum rates ranging from ~1 to ~1.8 µmol g⁻¹ sediment day⁻¹, and average rates generally following the expected kinetic temperature responses. Higher temperatures supported greater microbial biomass and community diversity, including a broader range of denitrifiers. Nevertheless, a single population, a novel strain of , dominated the community across all temperatures. Unlike the heterotrophic type strain, it possessed a conserved, likely transferable gene cluster for hydrogen oxidation, hydrogen sensing, and CO₂ fixation, as revealed by metagenomics. Although complete denitrifiers with hydrogen-oxidation capacity dominated at all temperatures, nitrite accumulated transiently below 15°C. The presence of aerobic respiration genes in all enriched denitrifiers indicates their ability to switch to oxygen as an electron acceptor if available. Our study demonstrates a notable presence of an autochthonous hydrogenotrophic denitrification potential in the studied aquifer and its effective stimulation by hydrogen injection alone. Furthermore, it identifies mass transfer constraints and redox control as targets for future process optimization.IMPORTANCENitrate contamination of groundwater remains a widespread challenge in agricultural regions, and effective remediation strategies are urgently needed. Hydrogen-stimulated chemolithoautotrophic denitrification represents a promising, low-carbon approach, yet its robustness under environmentally relevant temperature conditions has remained unclear. Our findings show that indigenous aquifer microorganisms can rapidly and consistently perform hydrogenotrophic denitrification across a broad temperature range (12-25°C), driven by a dominant species with a specialized hydrogen-oxidizing gene cluster. Identification of a conserved, potentially transferable gene cluster conferring chemolithoautotrophic lifestyle is not only biotechnologically interesting but also contributes to debate about genomic and ecological definitions of microbial species. This demonstrates that hydrogen injection can reliably activate existing microbial potential without requiring bioaugmentation and that temperature-driven shifts in community composition do not compromise nitrate removal. These insights advance the development of hydrogen-based bioremediation strategies for nitrate-impacted aquifers.
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3. {"_":"Pyridoxine can inhibit salvage of other B vitamers in .","sub":["6"],"i":["Salmonella enterica"]}
PMID:日期:2026-10-05Pyridoxal 5'-phosphate (PLP) is a nutrient required by all life. As an essential cofactor, PLP powers enzymatic reactions in primary and secondary metabolism. Microbes can obtain PLP through either PLP biosynthesis or the salvage of exogenous vitamin B. Vitamin B exists in multiple forms (vitamers) in the natural environment, but the effect of mixed B vitamer supplementation on microbial physiology has not been explored. In this study, the ability of to obtain PLP during growth in media containing various ratios of B vitamers is examined. The data show that the B vitamer, pyridoxine (PN), can inhibit the salvage of B vitamers, pyridoxamine and pyridoxal, by mutants. Genetic analyses were performed to determine the mechanism by which PN disrupts pyridoxamine and pyridoxal salvage. Mutants resistant to PN inhibition during the salvage of pyridoxamine were isolated and found to encode variants of B kinase (PdxK) with altered activity toward the B vitamer substrates. Suppressor analysis suggests that PN inhibition is, at least, partially due to competitive inhibition of the pyridoxamine kinase activity of PdxK by PN. Additionally, the data indicate that pyridoxine 5'-phosphate (PNP)-mediated toxicity also contributes to growth inhibition by PN. Without successful acquisition and utilization of vitamin B, microbes cannot survive or perform their essential roles in human health and the natural environment. Vitamin B exists in multiple forms (vitamers) in the natural world, but the effect of B vitamer mixtures on microbial growth has not been analyzed. This work shows that the B vitamers are not necessarily interchangeable sources of PLP, and they may have distinct effects on microbial physiology.
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4. {"_":"Autoinducer-2 functions as both a quorum-sensing and metabolic signal in .","i":["Escherichia coli"]}
4. {"_":"在**大肠杆菌**中，自诱导物-2既作为群体感应信号，也作为代谢信号发挥作用。","i":["Escherichia coli"]}PMID:日期:2026-10-05Bacteria integrate diverse environmental signals to coordinate behavior, yet the relationship between nutrient sensing and quorum sensing (QS) remains incompletely understood. Autoinducer-2 (AI-2) is unique among QS signals in that its production is tightly linked to central metabolism, raising fundamental questions about the boundary between metabolic and signaling functions. In , AI-2 coordinates collective behaviors through the operon, whose expression is controlled not only by the AI-2-responsive repressor LsrR but also by the cAMP receptor protein (CRP), placing it at the intersection of carbon sensing and population-level signaling. While inhibition of expression by PTS sugars was previously established, we show that non-PTS sugars similarly suppress expression through CRP, further decoupling QS activation from cell density and coupling it to carbon source availability. Genomic analysis across reveals that CRP binding sites in the promoter are broadly conserved, indicating that metabolic modulation of AI-2 signaling is an ancestral regulatory feature. Using a FRET-based biosensor, we further show that AI-2 uptake modulates intracellular cAMP levels similarly to non-PTS carbon transport, suggesting AI-2 may have originally functioned as a nutrient substrate. Additionally, we isolated soil- and phyllosphere-associated bacteria capable of utilizing AI-2 as a sole carbon source. Interestingly, this capacity seems to have evolved independently from the ability to produce and metabolize AI-2 through the LuxS/Lsr pathway. Overall, our findings reveal an underappreciated metabolic basis of AI-2 QS and suggest that, in some species, nutrient utilization and intercellular signaling may have diverged from a common ancestral pathway.IMPORTANCEQuorum sensing allows bacteria to coordinate collective behaviors by detecting secreted signaling molecules, yet the evolutionary origins of these systems remain poorly understood. AI-2, one of the most broadly conserved bacterial signals, is derived from central metabolism and processed by machinery in that strikingly resembles a sugar utilization system. Here, we show that nutrient availability overrides cell density as the primary determinant of AI-2 responsiveness, that this regulatory logic is conserved among genomes, and that environmental bacteria can grow on AI-2 as a sole carbon source. These findings reframe AI-2 as a signal embedded within, and potentially evolved from, nutrient-sensing pathways, with direct implications for understanding how byproducts of cellular metabolism can acquire signaling functions.
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5. {"_":"Survival, growth, and potential of horizontal gene transfer of antibiotic resistance genes of bovine on the cantaloupe surface.","i":["Salmonella"]}
5. {"_":"牛源抗生素抗性基因在哈密瓜表面的存活、生长及水平基因转移潜力。","i":["沙门氏菌"]}PMID:日期:2026-10-01outbreaks are often linked to contaminated cantaloupes, and bacterial multiple antibiotic resistance is frequently observed. Research is needed to understand the growth and survival of on cantaloupe surfaces, and the mechanisms of antibiotic resistance in that environment. This study investigated the growth, survival, and capacity for horizontal gene transfer of antibiotic resistance genes in on the surface of cantaloupe. Bovine-derived isolates were inoculated onto the surface of cantaloupe and incubated at 35°C under 65-75% humidity for 5 days. Each isolate was co-inoculated with donor CSH56 harboring helper plasmid RP4-8 and mobilizable plasmid pSC27, which carries kanamycin, neomycin, and ampicillin resistance genes. One hundred microliters of the suspension (1 × 10 CFU/mL) and 100 µL of the suspension (1 × 10 CFU/mL) was applied to each cantaloupe (1:10 ratio). survival and growth were monitored on Hektoen agar, transconjugants were enumerated on Hektoen agar with kanamycin, and donor were quantified on MacConkey agar. All isolates survived and exhibited growth within the 5-day period, with increases ranging from 0.94 log CFU/mL to 3.43 log CFU/mL. Notably, all isolates possess the capability to accept and integrate sequences derived from the pSC27 plasmid from into their chromosome, both and on cantaloupe. After conjugation, the count of mutants carrying the plasmid was 2.79 log CFU/mL . The survival, growth, and horizontal gene transfer of on the surface of cantaloupe pose significant risks to public health.IMPORTANCE, a leading cause of foodborne diseases, poses a global health threat, with multidrug resistance emerging as a severe problem. Cantaloupes have been implicated in salmonellosis outbreaks in multiple countries over the last three decades. We hypothesize that acquisition of antibiotic resistance genes from other bacteria may occur on the surface of the fruit. This study established that can survive, proliferate, and acquire antibiotic resistance genes on cantaloupe surfaces. This observation underscores the potential risks on the growth and transfer of genes between pathogens such as and in foods. This impact goes beyond disease production and contributes to the rapid escalation of antimicrobial resistance and transmission of virulence genes in foods and the environment, which is a significant threat to public health.
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6. {"_":"Formulations containing co-biotic compounds mediate microbiome function and composition without increased gas production in an gastrointestinal model.","i":["ex vivo"]}
PMID:日期:2026-10-01Dietary supplement formulations that contain co-biotics or compounds that modulate biological processes in both the host and microbiome to confer a health benefit are an emerging strategy to fine-tune both host physiology and gut microbiome function. Here, we evaluated three novel formulations containing co-biotic compounds (DM-02, a multivitamin; AM-02, formulated for energy and focus; and PM-02, formulated to promote sleep) for their effects on human gut microbiome composition and function. The three formulations and untreated control (Unt-Ctrl) were subjected to upper gastrointestinal digestion, after which the digesta were exposed to 24-h simulated colonic fermentation in the validated systemic intestinal fermentation research (SIFR) technology ( = 6 healthy adults). Outcomes included pH, short-chain fatty acid (SCFA) and gas production, ultra-deep metagenomic profiling, and untargeted metabolomics of post-colonic supernatants, each compared to Unt-Ctrl. All three formulations significantly reduced pH (3%-4%) and increased total SCFAs (9%-11%) and acetate (9%-12%), with AM-02 and PM-02 also increasing butyrate by 20% and propionate by 7%-8%, without increasing gas production. Each formulation significantly enriched specific SCFA- and B-vitamin-producing taxa. AM-02 significantly increased the abundance of two pyruvate fermentation to acetate/lactate pathways. PM-02 significantly increased the abundance of two tryptophan biosynthesis pathways, accompanied by an increase in available tryptophan and the abundance of tryptophan-producing microbes. All three formulations increased the availability of microbiome-derived metabolites, indicating microbiome functional modulation by the treatments. These findings support clinical evaluation of these novel formulations as a strategy to enhance microbiome composition and function. The gut microbiome produces metabolites, including short-chain fatty acids, B vitamins, and tryptophan derivatives, that are critical regulators of host physiology, from energy metabolism and gut barrier integrity to sleep and immune function. While probiotics introduce live microorganisms, and prebiotics selectively feed existing microbes, co-biotics represent a distinct category of compounds that simultaneously modulate host cell biology and microbiome activity. Despite growing interest in co-biotic supplementation, the impact of complete co-biotic-containing formulations on gut microbiome composition and functional output has not been evaluated. Using a validated gastrointestinal model inoculated with fecal microbiota from six healthy adults, we demonstrate that three supplement formulations containing co-biotic compounds consistently increased production of health-associated metabolites, selectively enriched beneficial microbial taxa, and modulated functional metabolic pathways, all without increasing gas production. These findings establish a mechanistic foundation for the clinical investigation of co-biotic formulations as targeted, tolerable interventions for optimizing gut microbiome function across diverse human populations.
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7. {"_":" volatiles elicit divergent behavioral responses in and .","i":["Pichia occidentalis","Drosophila suzukii","Drosophila melanogaster"]}
PMID:日期:2026-10-01(spotted-wing drosophila) is an invasive pest that infests ripe, market-ready fruit, posing a significant threat to fruit production. To develop alternatives to conventional chemical control, environmentally derived fungi (EF) were evaluated for their potential to modulate behavior and viability. , isolated from oral and fecal secretions, exhibited species-specific repellency in . In , exposure to reduced survival, most pronounced in males, and significantly inhibited female oviposition. In contrast, showed no reduction in survival and increased oviposition in the presence of the fungus. Solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME GC-MS) identified volatile metabolites produced by . Subsequent cafeteria-style assays of individual volatiles revealed that several of these compounds elicited aversive responses in while remaining attractive to , highlighting species-specific olfactory effects. Isoamyl butanoate, 2-phenyl-1-propanal, and benzyl alcohol demonstrated consistent repellent activity with superior efficacy compared to established repellents (2-pentylfuran, 1-octen-3-ol, and geosmin). Collectively, these findings demonstrate that EF metabolites can differentially influence closely related species and may be leveraged as targeted, behavior-based tools for managing .IMPORTANCE is a major invasive pest that damages fresh fruit by laying eggs in ripening crops, leading to significant agricultural losses. Current control strategies rely heavily on chemical insecticides, which provide incomplete control and can harm non-target organisms. Here, we show that volatile metabolites produced by the environmental yeast differentially influence closely related species, decreasing oviposition and longevity in while stimulating oviposition in . This species-specific divergence highlights the potential of microbial volatiles as precision tools for pest management, further supporting a behavior-based approach that selectively disrupts pest reproduction while minimizing ecological impact.
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8. {"_":"A phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan.","i":["Salmonella"]}
PMID:日期:2026-10-01Phage steering is an emerging antimicrobial strategy that exploits the evolutionary consequences of phage predation to sensitize bacterial populations to existing antimicrobials. While phage steering toward hypersensitivity to antibiotics and immune effectors has been well documented, little is known about whether a similar mechanism can enhance bacterial sensitivity to commercial disinfectants. Here, we show that SF1, a lytic siphovirus infecting , requires an intact O-antigen layer for adsorption and lytic replication, and that in-frame deletion mutants with O-antigen or deeper LPS defects had increased susceptibility to several commercial disinfectants. Critically, SF1 enhanced the activity of all disinfectants tested in combination assays and, moreover, exhibited mathematical synergy with benzalkonium chloride, hydrogen peroxide, and triclosan, compounds to which SF1-selected LPS mutants were hypersensitive. These findings are consistent with the hypothesis that SF1 functions not only as a bactericidal agent, but as an evolutionary steering tool that potentiates sanitizer activity through selection for sanitizer-hypersensitive phenotypes. Broadly, these results support phage steering as a promising strategy for deploying bacteriophages as adjuvants to commercial disinfectants.IMPORTANCECommercial disinfectants are essential for controlling pathogens throughout the food production and processing chain, but their effectiveness is increasingly compromised by bacterial tolerance and persistence. Although bacteriophages are being investigated as direct bactericidal agents, their utility as adjuvants to commercial sanitizers remains underexplored. In this study, we demonstrate that phage SF1 selects for LPS-deficient mutants that exhibit increased susceptibility to several commercial disinfectants. Importantly, we further show that SF1 synergizes with sanitizers to which phage-selected mutants are hypersensitive, supporting a steering-mediated mechanism whereby phage predation enhances sanitizer activity. To our knowledge, this is the first report demonstrating phage steering as a strategy for deploying bacteriophages as synergizing adjuvants to commercial disinfectants. These findings highlight the potential value of exploiting evolutionary trade-offs to improve pathogen control in food processing environments.
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9. {"_":"Regulation of central carbon metabolism and storage polymers by PTS in .","sup":["Ntr"],"i":["Rhizobium leguminosarum"]}
9. {"_":"根瘤菌中PTS对中心碳代谢和储存聚合物的调控。","sup":["Ntr"],"i":["豌豆根瘤菌"]}PMID:日期:2026-09-30The coordination of intracellular carbon and nitrogen levels is essential for optimal bacterial growth and, in rhizobia, for survival in the soil, root colonization, and symbiotic interactions with their host plants. Here, we show that the phosphotransferase system PTS, a global regulator of carbon and nitrogen metabolism, modulates the tricarboxylic acid (TCA) cycle activity and the accumulation of three major carbon storage polymers: glycogen, polyhydroxybutyrate, and exopolysaccharide in . The unphosphorylated form of the effector protein ManX is sufficient for the full activation of the TCA cycle dehydrogenase enzymes. Accordingly, loss of reduced dehydrogenase activity and redirected overflow carbon into storage polymers, phenocopying wild-type cells grown under nitrogen starvation. We further demonstrate that carbon metabolism in is also tightly regulated by the HPr kinase (HprK) protein. In an mutant, loss of NPr Ser48 phosphorylation favors phosphotransfer through the histidyl arm and is predicted to increase phosphorylation of downstream PTS components. When grown on glucose, the mutant accumulates elevated intracellular pyrimidine levels and exhibits increased malate dehydrogenase and TCA cycle activity, consistent with a misregulated shift toward gluconeogenesis. This mutant also overproduces exopolysaccharide, an effect potentially mediated by crosstalk between phosphorylated PtsN and the ChvI/ChvG two-component regulatory system. Together, these data suggest that HprK exerts pleiotropic control over processes regulated by PTS, influencing central metabolism while repressing exopolysaccharide production, likely by promoting the unphosphorylated state of PtsN.IMPORTANCEBacteria must continuously balance growth, nutrient availability, and storage to ensure fitness and survival in fluctuating environments. However, how regulatory networks integrate central metabolism with carbon storage remains poorly understood. This study reinforces the role of the PTS system as a central coordinator of carbon routing and polymer accumulation. By revealing how ManX and HprK-dependent phosphorylation influence central metabolism and the production of major carbon storage polymers in , we show that disruption of PTS signaling reprograms carbon allocation, uncoupling growth from carbon storage and mimicking nitrogen starvation even under nitrogen-replete conditions. These findings highlight a regulatory link between carbon-nitrogen signaling and bacterial storage strategies, with implications for cellular survival and plant symbiosis. More broadly, our work illustrates how global metabolic regulators shape bacterial physiological states, providing new insights into mechanisms that support microbial adaptation in complex environments.
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10. {"_":"Welcome to the house-warming party: social interactions and antagonism against in multi-species biofilms.","i":["Listeria monocytogenes"]}
10. {"_":"欢迎参加乔迁派对：多物种生物膜中的社会互动与对抗。","i":["单核细胞增生李斯特菌"]}PMID:日期:2026-09-30integrates into multi-species biofilms and persists in food processing facilities. Its persistence depends on the composition of the microbial communities, but those species that accommodate , or not, remain to be identified. Moreover, the decision-making on biofilm vs pellicle formation in microbial communities remains poorly understood. It was the aim of this study to identify communities that accommodate , or not, and to observe biofilm vs pellicle formation in multi-species biofilms. We quantified in six reconstituted communities, each consisting of 5-19 strains. A 15-member community was simplified into five species to quantify in all 31 combinations of these five strains. favored pellicle formation, while convinced others to join a surface-attached biofilm. In the six microbial communities, cell counts of were lowest when was also present. The antagonistic effect of was confirmed when analyzing all combinations of five strains. The presence of reduced cell counts of by about 1 log CFU/cm. also antagonized , but this was observed ( < 0.05) only in the absence of . Genome analyses of the community identified close to 100 different quorum-sensing systems, including peptides that are also bactericidal against . We conclude that the language used to describe human social interactions is also suited to describe microbial interactions in multi-species biofilm communities, but unraveling mechanisms of communication among biofilm-embedded bacteria remains subject to future studies. Microbial communication in relation to biofilm vs pellicle formation and on whether to accommodate s or not partially converge with quorum-sensing systems as a key mechanism. Two organisms, and , antagonized . Microbial interactions are strongly dependent on the complexity of the microbial community, and different behaviors were observed in biofilms formed by 2, 5, or 15 strains. Results presented in this study document that the use of protective cultures may also reduce persistence of in food processing facilities but are far from providing an understanding of communication and social rules in complex bacterial communities. The novel perspective on microbial interactions may direct future experiments.