MICROVASCULAR RESEARCH微血管研究
MICROVASCULAR RESEARCH(英文缩写 MICROVASC RES),ISSN 0026-2862,eISSN 1095-9319,中文译名:微血管研究 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.750 | Q2 |
| 2022 | 3.100 | Q2 |
| 2023 | 2.900 | Q2 |
| 2024 | 2.700 | Q2 |
| 2025 | 2.900 | Q2 |
MICROVASCULAR RESEARCH 最新收录文献
-
1. Shear stress and blood coagulation: A comparative study of smokers and diabetics, with healthy individuals.
PMID:日期:2026-11-01Blood coagulation is a complex physiological process governed by both biochemical and biomechanical mechanisms. While smoking, diabetes, and aging are established risk factors for thrombotic disorders, their combined interplay on shear-dependent coagulation behaviour remains poorly understood. This study investigates the effects of applied shear stress, smoking, diabetes, and age on blood coagulation using oscillatory rheometry to evaluate patient-specific haemostatic responses. Platelet-rich plasma (PRP) obtained from 30 volunteers representing healthy, smoker, and type 2 diabetic cohorts was analysed at 37 °C using an Anton Paar rheometer. Amplitude sweep experiments first established a linear viscoelastic region between 1 and 10 Pa, and subsequent rheological measurements were performed within a conservative stress range of 1-4 Pa. Time-to-gel point (TGP) was determined using the frequency-independent loss tangent criterion based on the Chambon-Winter gelation theory. Rheological findings were validated through conventional clotting time (CT) and prothrombin time (PT) measurements, while clot microstructure and platelet activation were further examined using field-emission scanning electron microscopy (FESEM) and fluorescence microscopy. A priori power analysis confirmed that the study was adequately powered to detect the observed intergroup differences. TGP decreased with increasing applied shear stress, demonstrating accelerated clot formation under higher mechanical loading. Compared with healthy controls, smokers and individuals with type 2 diabetes consistently exhibited shorter TGP, CT, and PT values, indicating enhanced coagulation potential. Older participants also demonstrated reduced TGP relative to younger healthy individuals, suggesting an age-related increase in procoagulant behaviour. FESEM revealed denser fibrin networks with reduced pore size at higher shear stresses, whereas fluorescence microscopy demonstrated increased CD62P expression, confirming progressive shear-induced platelet activation. These findings demonstrate that rheologically determined TGP is sensitive to both mechanical loading and underlying health status and, when complemented by structural and biological analyses, provides a quantitative marker for assessing patient-specific haemostatic function and thrombotic risk.
-
2. Evaluation of nailfold videocapillaroscopy findings in juvenile idiopathic arthritis.
PMID:日期:2026-11-01Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatic disease in children. This study aimed to evaluate nailfold videocapillaroscopy (NVC) findings in children with JIA, compare NVC findings across JIA subtypes, and assess their relationship with disease activity. This prospective cross-sectional study included patients diagnosed with JIA according to the International League of Associations for Rheumatology (ILAR) classification criteria who attended routine outpatient clinic visits between October 2025 and December 2025. NVC following the EULAR microcirculation protocol. NVC parameters were compared across JIA subtypes and against age-matched healthy reference data. The study included 104 patients (51.9% male), of whom 84 (80.8%) had a nonspecific pattern. None of the patients demonstrated a scleroderma pattern. Reduced capillary density was observed in 60.6% of the patients. Tortuous and crossing capillaries were present in all patients, while giant capillaries were not detected. Abnormal shapes, dilated capillaries, and microhaemorrhages were observed in 44.2%, 32.7%, and 25% of patients, respectively. Dilated capillaries were nominally more common in RF-negative polyarticular JIA (p = 0.045), though not after correction for multiple comparisons (q = 0.096). In comparisons with age-specific reference values, patients with JIA were found to have lower capillary density (p < 0.001), arterial (p < 0.001), venous (p < 0.001), and loop diameters (p < 0.001), capillary length (p = 0.003). No statistically significant correlations were observed between NVC parameters and disease duration or disease activity scores (p > 0.05). This study demonstrated significant microvascular differences in the NVC of children with JIA. However, largely similar NVC findings across JIA subtypes suggest subtype-specific differences are limited.
-
3. The effect of foot temperature on laser speckle contrast imaging (LSCI) and transcutaneous oxygen pressure (TcPO2) measurements.
PMID:日期:2026-11-01Assessment of foot microcirculation is increasingly implemented into clinical practice to assess vascular status and wound-healing potential in patients with vascular disease when conventional assessment techniques provide limited or unreliable information. Transcutaneous oxygen pressure (TcPO2) and laser speckle contrast imaging (LSCI) are techniques used to assess microcirculation in the foot. However, foot temperature is currently not considered during TcPO2 and LSCI measurements, despite substantial variation between patients and across measurements. Determining its influence is therefore crucial to ensure measurement reliability and reproducibility in clinical practice. This study aimed to determine the influence of foot temperature on TcPO2 and LSCI measurements. Physiological foot temperatures ranging from 25 °C to 35 °C were simulated in 30 healthy participants using cold and warm water immersion. Subsequently, plantar LSCI perfusion and skin temperature measurements were obtained, together with dorsal TcPO2 and skin temperature measurements around the electrodes. TcPO2 and LSCI mean perfusion values differed significantly between foot temperatures of 25 °C and 35 °C, with higher temperatures yielding higher values for both techniques. Despite these clear differences, interindividual variability was seen and no consistent relation was observed between temperature changes and corresponding TcPO2 values. LSCI perfusion followed an exponential relation with foot temperature, but prediction intervals were wide. Foot temperature following water immersion significantly influences TcPO2 and LSCI measurements, although temperature responses vary between individuals. For TcPO2, this effect remained present despite local electrode heating, indicating that surrounding foot temperature continues to affect measurements. Clinicians should therefore consider foot temperature when interpreting TcPO2 and LSCI measurements.
-
4. Ruxolitinib preserves endothelial function in middle-aged and old mice after ischemia/reperfusion injury.
PMID:日期:2026-11-01Ischemia/Reperfusion (I/R) and the increasing age of patients are coexisting challenges in today's cardiovascular medicine. Vascular I/R leads to endothelial dysfunction and is partially driven by inflammation. With increasing age, a profile of biomolecules known as the senescence-associated secretory phenotype (SASP) also increases. The SASP correlates with decreased resilience and intrinsic capacity to withstand various stressors and contains pro-inflammatory cytokines, likely fueling I/R injury. Senomorphics block SASP secretion. We investigated the effect of ruxolitinib on endothelial function after vascular I/R injury in middle-aged and old mice. Aortic rings of 11-13 and 23-25 months old C57BL/6 J mice were divided into three groups: Control (12 mo: n = 9; 24 mo: n = 10), ischemia/reperfusion (I/R group; 12 mo: n = 9; 24 mo: n = 11), and I/R with ruxolitinib (I/R + Ruxo; 12 mo: n = 13; 24 mo: n = 15). The aortic rings were stored in de-aired saline solution for 24 h without (I/R) or with ruxolitinib (I/R + Rux). Afterward, they were mounted in organ bath chambers. The reperfusion injury was induced by 200 μM sodium hypochlorite. In the Control group, no ischemia or sodium hypochlorite was performed. We investigated the vasomotor function using potassium chloride (KCl), phenylephrine (PE), acetylcholine (ACh), and sodium nitroprusside (SNP). The contraction to PE in % of KCl was higher in the I/R group (12 mo: 80 ± 10%; 24 mo: 94 ± 15%) compared to the control (12 mo: 51 ± 8%; 24 mo: 63 ± 13%) and lower in the I/R + Ruxo group (12 mo: 60 ± 9%; 24 mo: 70 ± 10%) compared to the I/R group, in both age populations. The maximal endothelial-dependent relaxation to ACh was substantially decreased in the I/R group (12 mo: 34 ± 6%; 24 mo: 34 ± 7%) compared to control (12 mo: 75 ± 5%; 24 mo: 88 ± 9%) and higher in the I/R + Ruxo group (12 mo: 59 ± 21%; 24 mo: 61 ± 12%) compared to I/R in both age populations. The endothelial-independent relaxation to SNP was comparable between all groups. The concentration of interleukin (IL)-1α, IL-7, IL-10, IL-13, and tumor necrosis factor-α was higher in the I/R + Ruxo group, only in 24-month-old mice. CC chemokine ligand (CCL)11, intercellular cell adhesion molecule-1, CCL8, RANTES, and C-X-C motif chemokine ligand 5 were comparable between I/R and I/R + Ruxo in 12- and 24-mo mice. Treatment of the aorta with the senomorphic agent ruxolitinib during ischemic storage protects the endothelial-dependent vasomotor function of the aorta in both age groups.
-
5. Evaluation of retinochoroidal microvascular parameters and biochemical markers in childhood obesity.
PMID:日期:2026-11-01To evaluate retinochoroidal microvascular changes and their association with biochemical parameters in children with obesity. This cross-sectional study included 100 children aged 6-15 years (50 obese, 50 normal weight). Retinal parameters superficial and deep capillary plexuses (SCP, DCP), choriocapillaris (CC), foveal avascular zone (FAZ), ganglion cell layer (GCL), and retinal nerve fiber layer (RNFL) were assessed using OCT and OCTA. Biochemical measures, including HDL, LDL, triglycerides, glucose, insulin, and blood pressure, were recorded. Associations between vascular density and biochemical variables were analyzed using regression analysis. Obese children showed a significantly smaller FAZ area than controls (262.11 ± 60.33 μm vs 316.47 ± 67.09 μm, p < 0.001). RNFL thickness was significantly reduced in the central region (9.35 ± 5.51 μm vs 11.25 ± 4.65 μm, p = 0.041). GCL thickness was significantly lower in the temporal (81.55 ± 10.07 μm vs 87.42 ± 9.81 μm, p = 0.022) and inferior regions (85.73 ± 13.29 μm vs 92.69 ± 10.06 μm, p = 0.028). No significant differences were observed in SCP, DCP, or CC vessel densities. Foveal SCP and DCP vessel densities were negatively associated with systolic blood pressure and positively associated with HDL cholesterol. Childhood obesity is associated with FAZ reduction and thinning of retinal neural layers. Retinal microvascular findings may reflect the influence of vascular and metabolic risk factors in pediatric obesity.
-
6. EV30 suppresses choroidal neovascularization associated with modulation of the mTOR/NF-κB/p38 MAPK signaling pathway.
PMID:日期:2026-11-01Wet age-related macular degeneration (wAMD) is a leading cause of irreversible vision loss characterized by pathological choroidal neovascularization (CNV). While anti-VEGF therapies are the standard of care, limitations such as treatment resistance and side effects necessitate novel therapeutic agents. This study evaluates the therapeutic efficacy and mechanism of EV30, a novel pterostilbene derivative, in suppressing CNV. EV30 was synthesized based on the pterostilbene template. In vitro, the effects of EV30 on human umbilical vein endothelial cells (HUVECs) proliferation, migration, and tube formation were assessed using Cell Counting Kit-8 (CCK-8), scratch wound, and tube formation assays, respectively. Mechanistic pathways were investigated via Western blotting and RT-qPCR. In vivo, a laser-induced CNV mouse model was treated with intravitreal EV30. Efficacy was evaluated utilizing fundus photography, fluorescein angiography (FFA), optical coherence tomography (OCT), and choroidal flat mounts (IB4 staining). Finally, biosafety was assessed through histology (H&E), electroretinography (ERG), and blood analysis. EV30 demonstrated potent anti-angiogenic properties in vitro, significantly inhibiting HUVEC proliferation, migration, and tube formation in a dose- and time-dependent manner. EV30 reduced vascular endothelial growth factor A (VEGFA) expression and modulated the phosphorylation status of proteins associated with the mTOR/NF-κB/p38 MAPK signaling pathway. In the laser-induced CNV model, EV30 effectively reduced lesion area and vascular leakage comparable to bevacizumab. Furthermore, ERG analysis revealed that EV30 partially preserved retinal electrophysiological function, as indicated by improved scotopic a-wave amplitudes, suggesting functional protection of the retina in the CNV model. EV30 exerted anti-angiogenic effects and was associated with modulation of mTOR/NF-κB/p38 MAPK signaling activity. Together, these findings suggest that EV30 represents a potential therapeutic candidate for CNV by suppressing pathological angiogenesis and modulating inflammation-associated signaling pathways.
-
7. Forsythoside E alleviates chronic unpredictable mild stress-induced depressive-like behaviors in mice: associations with blood-brain barrier integrity and neuroinflammatory changes in the prefrontal cortex.
PMID:日期:2026-11-01Persistent stress-related depression has increasingly been associated with neuroinflammatory activation and impairment of blood-brain barrier (BBB) function. Forsythoside E (FE), a phenylethanoid glycoside isolated from Forsythia suspensa, is known to exert anti-inflammatory and cytoprotective effects. Given that its specific role in stress-related depressive states and associated BBB abnormalities remains insufficiently defined, this study aimed to evaluate whether FE could mitigate behavioral impairment and modulate the neurovascular inflammatory microenvironment in a chronic stress model. Male C57BL/6 mice were subjected to chronic unpredictable mild stress (CUMS) for 6 weeks, with FE (50 mg/kg/day) administered orally starting from day 21. Behavioral alterations were evaluated using the sucrose preference, novelty-suppressed feeding, tail suspension, forced swimming, and open-field paradigms. ELISA, immunofluorescence, and Evans blue extravasation were employed to quantify inflammatory changes, BBB leakage, and perivascular IL-17A and VEGFA expression in the prefrontal cortex (PFC). FE markedly attenuated CUMS-induced behavioral abnormalities, including reduced sucrose preference, prolonged feeding latency, increased immobility, and diminished central exploration, though body weight loss was not significantly corrected. FE treatment was associated with reduced TNF-α, IL-6, IL-1β, and IL-17A levels in the PFC, reduced microglial accumulation, decreased Evans blue extravasation, and reduced perivascular IL-17A and VEGFA expression. FE was associated with alleviation of depressive-like behaviors in CUMS-exposed mice. These effects are accompanied by reduced neuroinflammatory responses, decreased BBB permeability, and alterations in IL-17A and VEGFA expression in the prefrontal cortex. These findings suggest a potential involvement of neurovascular and inflammatory processes in the observed effects of FE, although causal mechanisms require further investigation.
-
8. Exercise-induced redox modulation of red blood cell function in health and disease: Mechanistic implications for hemoglobin cycling and microvascular regulation.
PMID:日期:2026-11-01Exercise generates transient systemic redox and hemodynamic signals that influence oxygen delivery, vascular tone, and metabolic adaptation in both health and disease. Red blood cells (RBCs) are increasingly recognized as regulated redox-responsive cells rather than passive oxygen carriers. This narrative review integrates mechanistic and translational evidence linking RBC deformability, microvascular transit, and perfusion matching to exercise-induced reactive oxygen and nitrogen species (RONS), hemodynamic shear, hemoglobin redox cycling, antioxidant buffering, and nitric oxide (NO)-related signaling. RBC redox architecture, supported by glutathione and thioredoxin systems maintained by pentose phosphate pathway-derived nicotinamide adenine dinucleotide phosphate (NADPH), limits membrane lipid and protein damage during recurrent oxidative oscillations and restrains hemoglobin auto-oxidation. Exercise-induced changes in red blood cell nitric oxide synthase (RBC-NOS) activity, S-nitrosylation chemistry, nitrite reduction, and adenosine triphosphate (ATP)-mediated purinergic signaling may further influence NO bioavailability and downstream endothelial responses. However, severe, prolonged, unaccustomed, or insufficiently recovered oxidative stress may shift these adaptive responses toward methemoglobin accumulation, band-3 aggregation, vesiculation, phosphatidylserine-positive erythrocyte clearance, hemolysis, and impaired microvascular regulation. We therefore propose an intensity-duration-recovery framework in which moderate, transient, and adequately recovered redox/shear pulses support RBC deformability and microvascular adaptation, whereas prolonged or repeatedly performed high-intensity exercise may exceed erythrocyte buffering capacity and promote hemolytic injury or premature erythrocyte clearance. Because RBC redox dysfunction overlaps with cardiometabolic, vascular, hematological, and exercise-intolerance-related disease settings, standardized RBC-contained redox biomarkers may help improve the interpretation of exercise responses and support phenotype-aware exercise prescription.
-
9. Microcirculatory effects of heparin: Evidence for endothelial glycocalyx degradation in children and young adults.
PMID:日期:2026-11-01Heparin, a widely used anticoagulant in acute care, is known to interact with and modulate the endothelial glycocalyx (EG). This interference becomes particularly relevant in conditions such as sepsis, where microvascular dysfunction and endothelial degradation contribute substantially to organ failure. However, the immediate effects of heparin on microcirculatory parameters in humans remains insufficiently characterized. This study investigates the acute effects of heparin on microcirculation and EG integrity in-vivo using darkfield microscopic imaging. This pre-postinterventional study includes 34 patients (mean age 11.8 ± 10.2 years; 53% female) who underwent cardiac catheterisation receiving a standardized heparin bolus. Before and 15 min after administration of heparin we measured sublingual microcirculation and EG using the GlycoCheck® (Version 5.2) system with Sidestream Dark Field (SDF) imaging. Red blood cell flow (RBC Flow), perfused microvessel density (PVD), capillary blood volume (CBV), and perfused boundary region (PBR), a surrogate for glycocalyx integrity, were assessed. Heparin administration was associated with a significant increase in PBR, indicating EG degradation and a concomitant reduction in PVD, while RBC Flow and CBV remained unchanged. These effects persisted after adjustment for systemic hemodynamic and respiratory changes and were independent of baseline hemoglobin, hematocrit and blood glucose levels. These findings provide insights into the short-term effects of heparin on EG integrity and microcirculation and highlight potential for unintended microvascular effects of heparin. Further studies are warranted to investigate drug-induced microvascular alterations and their long-term clinical consequences. German Clinical Trials Register, DRKS00035631 https://www.drks.de/drks_web/.
-
10. Viscoelastic hemostatic assays in trauma-induced coagulopathy: Precision transfusion, fibrinolytic phenotyping, and future directions.
PMID:日期:2026-09-19Trauma-induced coagulopathy (TIC) is a rapidly evolving hemostatic disorder that develops early after severe injury and remains a major determinant of hemorrhagic mortality and thrombotic complications. Conventional coagulation tests provide limited information on the dynamic interactions among coagulation factors, platelets, fibrinogen, and fibrinolysis, often delaying targeted intervention. Viscoelastic hemostatic assays (VHAs), thromboelastography and rotational thromboelastometry, offer real-time whole-blood assessment of clot initiation, propagation, strength, and lysis. This narrative review synthesizes evidence on TIC pathophysiology, emphasizing shock-induced endotheliopathy, activated protein C signaling, platelet dysfunction, and the fibrinolytic spectrum. VHA-guided transfusion is examined against fixed-ratio protocols and low-titer group O whole blood. Equal attention is given to the boundaries of these assays. Because VHAs are static, low-shear tests performed without a vascular surface, they do not interrogate platelet adhesion and cannot detect the receptor shedding characteristic of exhausted platelet syndrome; a normal clot strength with an adequate platelet count may therefore coexist with impaired platelet plug formation. Initiation parameters are sensitive to acidosis, hypothermia, and endogenous heparinoids independently of factor concentration, and conventional assays cannot distinguish pathologic fibrinolytic shutdown from hypo-fibrinolysis. Two constraints limit generalizability of published thresholds. Parameters are not interchangeable between manufacturers or between device generations, so thresholds cannot be transferred, a limitation that also constrains meta-analysis. Reference values differ systematically by sex and age, and current trauma thresholds derive from predominantly male cohorts. Emerging approaches include provocative tissue plasminogen activator-challenged testing, dielectric coagulometry, and flow-based microfluidic assays that interrogate adhesion under shear.