CRYOBIOLOGY低温生物学
CRYOBIOLOGY(英文缩写 CRYOBIOLOGY),ISSN 0011-2240,eISSN 1090-2392,中文译名:低温生物学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.728 | Q3 |
| 2022 | 2.700 | Q2 |
| 2023 | 2.300 | Q2 |
| 2024 | 2.100 | Q2 |
| 2025 | 2.600 | Q2 |
CRYOBIOLOGY 最新收录文献
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1. Natural mating with sterile triploid males after artificial insemination recapitulates manual sperm mixing to restore the fertility of cryopreserved semen in Bombyx mori.
PMID:日期:2026-09-24Silkworm sperm exist as two types: eupyrene and apyrene. Apyrene sperm lack nuclei and are incapable of fertilization. However, they play a crucial role in facilitating fertilization by assisting nucleated sperm in migrating from the bursa copulatrix to the spermatheca. Additionally, apyrene sperm exhibit lower cryotolerance compared with eupyrene sperm, resulting in significantly reduced fertility following cryopreservation. Conventionally, the fertility of cryopreserved semen has been restored by mixing it with semen from sterile triploid males, which contains functional apyrene sperm and nonfunctional eupyrene sperm. However, collecting semen from triploid males is labor-intensive. To reduce this effort, we investigated whether mating with triploid males could improve the fertility of cryopreserved semen without artificial mixing with semen from triploid males. The results of this study demonstrated that females mated with triploid males and artificially inseminated with cryopreserved semen produced more fertilized eggs compared with unmated females. Furthermore, females mated with triploid males after artificial insemination (AI) with cryopreserved semen laid more fertilized eggs and exhibited a significantly higher fertilization rate than those mated before AI with cryopreserved semen. Notably, injecting 5 μL of cryopreserved semen produced more fertilized eggs than 10 μL when females were mated with triploid males after AI. These findings suggest that mating with triploid males eliminates the need for semen collection and permits AI with smaller volumes of cryopreserved semen.
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2. Tail-degloving and cold storage facilitate primary cell culture from wild-caught rodents.
PMID:日期:2026-09-17The use of wild rodents as biological resources is hindered by the difficulties of live transport and primary culture contamination caused by skin bacteria. To address this, we developed a "tail-degloving" method to physically remove the contaminated external integument. Using the large Japanese field mouse (Apodemus speciosus), this method suppressed contamination compared to conventional biopsies, yielding cells within 5-7 days that were capable of subculturing and cryopreservation. The protocol withstood 4°C storage for up to 7 days and enabled contamination-free cell establishment from a large rodent. This tail-degloving protocol eliminates live transport risks and effectively establishes primary cells after a multi-day transport delay.
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3. Rapid warming improves blastocyst production of vitrified bovine oocytes while significantly affecting mitochondrial activity.
PMID:日期:2026-09-14Improvement of bovine oocyte vitrification focuses mainly on the cooling step, while warming steps receive little attention. Recent studies report improved in vitro embryo production (IVEP) efficiency in vitrified rodent embryos warmed rapidly at high temperatures (50-60°C). This effect has not yet been tested on bovine oocytes; therefore, this study aimed to evaluate rapid warming's influence on mitochondrial functionality, nuclear DNA integrity, and IVEP efficiency. Three groups of in vitro matured oocytes were included: 1) control, 2) SW - vitrified oocytes warmed slowly, and 3) RW - vitrified oocytes warmed rapidly. Oocytes were assessed in two experiments for: (1) embryo production and (2) mitochondrial functionality and DNA integrity using MitoTracker® Red CMXRos and Hoechst33342 dyes. All vitrified-warmed oocytes were examined for cytoplasm expansion and rupture after warming. In Experiment 1, cleavage rates differed significantly among all groups, with RW showing higher rates than SW. In Experiment 2, the strongest MitoTracker® Red CMXRos fluorescence was observed in the control group, followed by SW and RW. No significant differences were found between SW and RW in DNA degeneration, cytoplasm expansion, or rupture. Although mitochondrial mass was lowest in RW, higher temperature did not increase DNA damage or affect cytoplasm expansion compared to SW. Moreover, RW achieved the best cleavage and blastocyst rates among vitrified-warmed groups. These findings indicate that rapid warming does not compromise oocyte integrity and may improve IVEP outcomes. The data suggests potential for incorporating rapid warming into vitrification protocols for mature bovine oocytes.
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4. DMSO and Serum-free cryopreservation of T-cells enabled by glycerol/betaine formulation and controlled-rate freezing with geometry-matched thermal contacts.
PMID:日期:2026-09-09T-Cells are key components of the adaptive immune system and engineered (e.g CAR-T) T-cells are deployed in immunotherapy, particular for haematologic malignancies. Methods to replace DMSO and serum during T-cell cryopreservation are crucial for both therapies and basic research to improve recovery and function. Glycerol and osmolytes are promising alternatives owing to their lower cytotoxicity than DMSO but often fail to give robust post-thaw recovery. Here we explore the combination of glycerol and betaine for the cryopreservation of Jurkat cells and demonstrate a significantly improved cryopreservation process that is suitable for both low-volume multi-well plate and vial-based freezing with no added serum. The optimised formulation of 10% glycerol and 2% betaine gave >90% post-thaw recovery, but crucially this was achieved when linked with a 3 °C/min freezing rate, and it was observed that faster freezing in general outperformed slower (1 °C/min) which is in contrast to standard protocols which rely on passive cooling devices. The increased rate of cooling appeared to mitigate delayed nucleation damage, which is typically associated with multi-well plate freezing, and the solutions were shown to not impact nucleation in droplet freezing assays. These results demonstrate an easily useable formulation for routine banking, particularly in multiwell-plates which is essential for assay-ready/high-throughput screening approaches as well as vials for larger-volume applications. The removal of serum may also facilitate shipping, where country-specific guidelines for animal-based serum prevent import/export as well as reducing costs.
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5. Development of techniques for cryopreserving giant clam larvae.
PMID:日期:2026-09-01Giant clams (Tridacna spp.) are keystone reef organisms that play important ecological roles in Indo-Pacific coral reef ecosystems. However, their populations have declined substantially because of overexploitation, habitat degradation, and climate change. Cryopreservation offers a potential strategy for preserving genetic resources and supporting future conservation and restoration efforts. In this study, we evaluated the feasibility of cryopreserving D-stage larvae of Tridacna crocea and T. maxima using a two-step freezing protocol. The effects of different cryoprotectants, concentrations, and equilibration times on post-thaw larval integrity and motility were assessed. Larval development was monitored using microscale culture techniques and microscopic observations. The results demonstrated species-specific responses to cryopreservation treatments, with T. maxima generally exhibiting greater cryotolerance than T. crocea. Ethylene glycol produced the highest larval integrity percentage (LIP) in both species. In addition, glycerol and propylene glycol yielded relatively high LIP values in T. crocea, whereas methanol and dimethyl sulfoxide (DMSO) performed more effectively in T. maxima. Post-thaw motility was observed only in a limited number of treatment groups, with the highest motility recorded in larvae treated with 0.75 M glycerol for 30 min and 0.5 M DMSO for 20 min. In contrast, non-cryopreserved control larvae exhibited a progressive decline in motility during culture, decreasing to 12.1% in T. maxima and 4.0% in T. crocea after 24 h. To our knowledge, this study represents the first investigation of cryopreservation in tridacnid D-stage larvae. Although post-thaw motility remained limited, the successful preservation of larval structural integrity demonstrates the potential of cryopreservation as a conservation tool for giant clams. Further optimization of species-specific cryopreservation protocols will be necessary to improve post-thaw recovery and support long-term genetic resource banking.
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6. Vacuum-assisted sucrose impregnation improves frozen pineapple quality through water-state modification.
PMID:日期:2026-09-01Freezing-induced ice recrystallization is a major limitation in preserving the quality of high-moisture fruits because of its adverse effects on cellular structure, texture, and nutritional stability. This study investigated vacuum-assisted sucrose impregnation as a cryostabilization approach for improving the quality of frozen pineapple stored at -18 °C for 21 days. Pineapple cubes were subjected to untreated control, atmospheric sucrose impregnation (AI; immersion in 60% (w/w) sucrose solution for 30 min at atmospheric pressure), and vacuum-assisted impregnation (VI; 31.8 kPa for 20 min followed by 10 min relaxation). Mass transfer, thermal characteristics, physicochemical properties, color, texture, and microstructural changes were evaluated. Vacuum treatment significantly enhanced solute incorporation, increasing cryoprotectant uptake to 11.2%. Differential scanning calorimetry revealed that VI reduced the onset freezing temperature from -1.8 to -3.6 °C and decreased melting enthalpy by approximately 52% compared with untreated samples. Freezable water content decreased from 72.4% to 38.6%, while bound water increased from 27.6% to 61.4%, indicating enhanced water immobilization within the fruit matrix. During frozen storage, VI-treated samples exhibited lower ion leakage (9.1%), greater retention of total phenolic content (67.5 mg GAE/100 g), higher antioxidant activity determined by DPPH scavenging (73.9%) and FRAP (581.3 μmol Fe equivalents/100 g fresh weight), and improved textural stability, maintaining hardness at 21.4 N compared with 10.2 N in control samples after 21 days. SEM observations further confirmed reduced tissue disruption and preservation of cellular architecture. These findings demonstrate that vacuum-assisted sucrose impregnation effectively improves cryostability by regulating water distribution and preserving structural integrity in frozen pineapple.
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7. A fast and simple one-step oocyte vitrification and warming protocol: proof-of-concept validation in the mouse model.
PMID:日期:2026-09-01Oocyte vitrification is widely used in fertility preservation and assisted reproductive technologies (ART), but current protocols rely on sequential cryoprotectant exposure steps that are labor-intensive and operator-dependent. This study evaluated the feasibility of a simplified one-step vitrification and warming protocol using mouse metaphase II oocytes. An initial optimization phase using 716 oocytes identified the optimal exposure time to the newly developed vitrification and warming solutions. Subsequently, 876 oocytes were allocated to fresh controls, standard multi-step vitrification, or the optimized one-step protocol. Outcomes included post-warming survival, meiotic spindle integrity, chromosome alignment, reactive oxygen species (ROS) levels, mitochondrial distribution, intracytoplasmic sperm injection (ICSI) outcomes, blastocyst development, total blastocyst cell counts, and full-term development after embryo transfer. An one-step exposure time of 2 min achieved the highest post-warming survival (100%), comparable to the standard protocol (98.9%; p = 0.080). Immunofluorescence analysis demonstrated normal spindle morphology, chromosome alignment, oxidative stress levels, and mitochondrial distribution across groups. Survival after warming and ICSI, as well as blastocyst formation rates, did not differ significantly between vitrification methods and fresh controls (p > 0.05). Although blastocyst total cell numbers were slightly reduced in vitrified groups, full-term development rates were comparable among all groups, and offspring developed normally to adulthood. These findings demonstrate that one-step vitrification and warming can preserve oocyte developmental competence while substantially simplifying cryopreservation procedures, supporting its potential as a practical alternative to conventional vitrification protocols in ART.
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8. Postprocedural pain after microwave ablation versus cryoablation for pulmonary tumors: A retrospective comparative study.
PMID:日期:2026-09-01This retrospective single-center study compared recorded postprocedural visual analog scale (VAS) pain scores after microwave ablation (MWA) and cryoablation for pulmonary tumors under routine postoperative care. Patients who underwent CT-guided pulmonary tumor ablation between January 2019 and October 2025 were included. Pain scores were recorded by thoracic surgery ward nurses using a VAS at 8, 16, 24, and 48 h after the procedure, and generalized estimating equations were used to account for repeated observations. A total of 68 ablation sessions, including 41 MWA and 27 cryoablation sessions, were included for pain analysis. Mean VAS scores were lower after cryoablation than after MWA at 8 h (0.5 ± 1.3 vs. 1.5 ± 1.6), 16 h (0.9 ± 1.8 vs. 2.0 ± 1.8), 24 h (0.3 ± 0.6 vs. 1.7 ± 1.8), and 48 h (0.3 ± 0.6 vs. 1.1 ± 1.3). Cryoablation was associated with lower recorded VAS scores than MWA from 8 to 48 h after pulmonary tumor ablation under routine postoperative care. These findings suggest that cryoablation may be associated with lower recorded postoperative pain scores, although MWA remains a feasible treatment option for pulmonary tumors.
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9. Microbiological findings in cryocontainers and ultra-low temperature freezers and the source of contamination.
PMID:日期:2026-09-01Cryogenic liquid nitrogen (LN) storage containers are widely used for the long-term preservation of cells and tissues, yet they may represent an overlooked source of microbial contamination. Effective contamination control within cryogenic systems and the surrounding cryobank environment is essential, particularly when supporting clinical cell or tissue transplantation. Contamination risk increases in the absence of adequate air filtration and is further amplified by high humidity condensation leading to ice accumulation, frequent container opening, insufficient packaging of stored materials, and improper handling during loading and unloading procedures. This study evaluated microbial contamination in LN vapor-phase containers (T ≤ -150 °C) using standard cultivation methods and MALDI-TOF mass spectrometry, with emphasis on the potential spread of microorganisms via LN vapor. Contamination levels in LN containers were compared with those in mechanical low-temperature freezers (T ≤ -80 °C), alongside microbial profiles detected on protective gloves used during sample manipulation. Microbial growth (2-10 CFU/mL), based on analysis of the melting ice, was detected in 5 of 8 LN containers. Detected organisms included potential pathogens such as Delftia tsuruhatensis, Acinetobacter spp., Pseudomonas spp., and Staphylococcus aureus, as well as non-pathogenic environmental species. Wall swabs revealed additional bacteria, while all lid swabs remained culture-negative. Mechanical freezers exhibited low-level contamination (1-14 CFU/mL), with microbial profiles overlapping those detected in LN systems. Matching organisms found on gloves indicate operator handling as a major contamination route. Microbial presence in frost and meltwater further suggests possible transmission via LN vapor. These findings underscore the need for stricter hygiene measures, improved decontamination procedures, and routine microbiological monitoring in cryogenic storage environments.
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10. In vitro embryo production in alpacas (Lama pacos): Blastocyst development from oocytes vitrified by Microdrop or Cryotech technique.
PMID:日期:2026-09-01We evaluated the use of alpaca oocytes vitrified using the Microdrop or Cryotech technique for in vitro embryo production. Grade I-II COCs (n = 1094) were recovered from slaughterhouse ovaries, and in vitro matured for 36 h. Then, MII oocytes were randomly distributed in groups: a) vitrified using two techniques [Microdrop (VM group; n = 250) or Cryotech (VC group; n = 244)], b) just exposed to the corresponding vitrification/thawing solutions (EM group; n = 200 or EC group; n = 200) or c) non-exposed nor vitrified (i.e. fresh oocytes; control group; n = 200). After treatment administration oocytes from all groups were IVF using epididymal sperm and in vitro cultured for up to 8 days. The vitrification technique and the exposure to vitrification solutions of MII oocytes negatively affected (p < 0.001) the cleavage rate compared to control group. On the other hand, exposure to Microdrop vitrification solution (EM group) and vitrification using the Microdrop technique significantly (p < 0.001) reduced the embryo developmental rate at all stages evaluated. Early blastocysts rate was affected (p < 0.001) by treatment, and it was greatest (p < 0.001) for the EC and VC, intermediate (p < 0.05) for the control, and lowest (p < 0.001) for VM and EM groups. Expanded and hatched blastocyst were not observed in the groups where oocytes were subjected to either Cryotech or Microdrop vitrification technique. We conclude that Cryotech is a more suitable vitrification technique for MII alpaca oocytes than Microdrop, under the conditions used in this study (timing and cryoprotectants concentration), allowing the development of early blastocyst at a rate comparable to control group.