CANADIAN JOURNAL OF MICROBIOLOGY加拿大微生物学杂志
CANADIAN JOURNAL OF MICROBIOLOGY(英文缩写 CAN J MICROBIOL),ISSN 0008-4166,eISSN 1480-3275,中文译名:加拿大微生物学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.226 | Q3 |
| 2022 | 2.800 | Q3 |
| 2023 | 1.800 | Q4 |
| 2024 | 1.600 | Q4 |
| 2025 | 2.400 | Q3 |
CANADIAN JOURNAL OF MICROBIOLOGY 最新收录文献
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1. Coincidental observation of a parvovirus during a targeted bacterial survey of cattle suffering from septic arthritis.
PMID:日期:2026-09-24DNA capture-based sequencing by hybridization probe enrichment (CapSeq) affords high-throughput and targeted gene-to-organism detection. Despite the specificity designed into CapSeq platforms, untargeted DNA represent additional genes and organisms of interest. To this end, de novo metagenome assemblies of CapSeq datasets present opportunities for discovery. We report an example here. CapSeq of samples that originated from cattle suffering from septic arthritis resulted in detection and assembly of two ungulate tetraparvovirus 1 (UTPV1) genomes. These coincidental detections and assemblies were obtained from a platform designed to characterize bacterial, not viral, pathogens. Access to a sample matched shotgun metagenomic dataset corroborated the result. Importantly, septic arthritis is a significant source of lameness in beef cattle that lacks effective and approved treatments, and the list of aetiological agents is incomplete. This metagenomic observation calls attention to the possible implication of viral contributors to bovine septic arthritis, which is treated as a bacterial disease. The results exemplify the potential application of unbiased CapSeq data mining to support clinical discoveries.
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2. The blue light receptor gene PoWC-1 plays a role in the formation of Pleurotus ostreatus primordia.
PMID:日期:2026-08-31Blue light is a critical environmental signal that triggers fruiting body initiation in mushroom-forming fungi. Pleurotus ostreatus is a widely cultivated model mushroom, yet the molecular mechanisms linking light perception to primordia formation remain poorly understood. Here, we functionally characterized the blue light receptor gene PoWC-1, a homolog of Neurospora crassa white collar 1, during early morphogenesis. Antisense-mediated silencing of PoWC-1 caused a marked delay in primordia formation under light/dark cycles and complete arrest at the undifferentiated mycelial stage under continuous darkness, underscoring its role in developmental progression. Even in darkness, PoWC-1 silencing downregulated key developmental transcription factors, including hom1, hom2, Pofst3, and fst4, suggesting that PoWC-1 may contribute to developmental competence independently of light. Comparative transcriptomics further revealed that PoWC-1 influences a range of metabolic pathways-such as glycolysis/gluconeogenesis, the pentose phosphate pathway, and amino sugar metabolism-that may support the energetic and biosynthetic demands of fruiting initiation. In addition, PoWC-1 appears to affect upstream signaling components, including the MAPK pathway and the two-component system, along with a suite of downstream transcription factors. These findings suggest that PoWC-1 functions as a key regulator integrating blue light signals into a multi-layered network involved in metabolic reprogramming, signal transduction, and transcriptional regulation during primordia formation in P. ostreatus. Collectively, this study provides a molecular framework for understanding photomorphogenesis in edible mushrooms.
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3. Contrasting growth responses of early- and late-successional native grasses to AMF-rich whole soil inocula under greenhouse conditions.
PMID:日期:2026-08-27Arbuscular mycorrhizal fungi (AMF) form symbioses in terrestrial ecosystems, yet plant responses to AMF-rich soil inocula are variable and do not necessarily reflect root colonization. We investigated how plant successional strategy and AMF-rich soil inocula interact to drive plant performance and root colonization structures. In a greenhouse experiment, four early- and late-successional native grasses were grown with five trap-cultured AMF-rich whole soil inocula. We assessed responses in biomass and height, expressed as log response ratios relative to sterile controls, alongside intraradical hyphal and arbuscular colonization. Biomass responses were generally negative and depended on the interaction between inoculum treatment and successional group. Late-successional species exhibited less negative growth responses than early-successional species. The probability of detecting hyphal colonization differed among inoculum treatments, whereas arbuscular colonization depended on the interaction between inoculum and successional group. However, neither arbuscules nor hyphal colonization predicted biomass responses, showing that greater physical colonization does not necessarily translate into greater plant growth. These findings show that structural colonization alone is insufficient to predict the growth effects of AMF-rich whole soil inocula. For grassland restoration, our results highlight that evaluating soil inocula based solely on physical colonization is insufficient; successful management requires assessing their functional effects on target native grasses.
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4. Peptidylarginine Deiminase Inhibitor Treatment is Associated with Increased Phage Susceptibility in Erwinia amylovora.
PMID:日期:2026-08-26Understanding the phage resistance mechanisms in Erwinia amylovora is crucial for improving phage therapy applications targeting this plant pathogen, which is responsible for fire blight disease in apple and pear orchards. This study investigated E. amylovora phage resistance in the presence of peptidylarginine deiminase (PAD) inhibitors, which have been found in other studies to prevent the conversion of arginine to citrulline as a post-translational modification that may initiate outer membrane vesicle (OMV) formation. Infection of the E. amylovora Ea6-4 strain by ΦEa21-4 phages, in the presence of either GSK199 or Cl-Amidine, demonstrated higher infection efficacy and larger phage lysis areas compared to the control infections without PAD inhibitors. Our data suggests that E. amylovora could conceivably employ OMVs as a possible passive phage resistance mechanism in addition to the previously identified regulator of capsule synthesis, the two-component Rcs-based phage resistance system.
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5. Global Report on High Pathogenicity Avian Influenza in Antarctica and sub-Antarctic Islands.
PMID:日期:2026-06-23Geographical isolation and extreme climates have resulted in the long isolation of Antarctica and sub-Antarctic islands. The result is a unique assemblage of animals, many endemics to the region. Low pathogenicity avian influenza is present in Antarctica; however, we have a poor understanding of the epidemiology, ecology and evolution of these viruses in the region. Of concern is the arrival of high pathogenicity avian influenza (HPAI) H5N1 clade 2.3.4.4b in the austral summer of 2023/24, and subsequent range expansion in the 2024/25 austral summer. Herein we review data and information from the literature (including all pre-prints, reports, and press releases), providing a narrative of events in the sub-Antarctic and Antarctica. We also address the impacts of HPAI H5N1 on Antarctic wildlife, and the unique challenges of working in the region.
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6. Performance Comparison of Commercially Available Kits for Wild Birds RNA Extraction.
PMID:日期:2026-06-09RNA extraction demands careful sample handling and processing due to the lack of stability of RNA in the environment. However, these conditions are sometimes challenging to achieve when working with free-ranging animals. The main objective of this study was to evaluate the efficiency of three commercial RNA extraction kits for biological samples obtained from 6 wild bird species (black-headed gull, griffon vulture, house sparrow, mallard, song thrush and common starling). We extracted 24 samples (faeces, oropharyngeal swabs, and cloacal swabs) using RNeasy PowerMicrobiome Kit, RNeasy PowerFecal Pro Kit, and RNeasy Mini Kit (Qiagen). The extraction performance was assessed based on total RNA yield, RNA integrity, and purity ratios (260/280 and 260/230). Moreover, an analysis to determine the inhibitory effect of the extracted samples and a multiple linear regression model were performed.RNA extraction performance varied considerably among species, sample types and extraction kits. Overall, the RNeasy PowerFecal Pro Kit yielded higher RNA integrity and purity values and showed lower RT-qPCR inhibition than the other kits, particularly in faecal samples. These findings highlight the importance of selecting extraction methods according to both species and sample type when working with wildlife samples.
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7. Evaluating how infectious bursal disease virus influences influenza A virus transmission in chickens.
PMID:日期:2026-06-09Infectious bursal disease virus (IBDV) is a major cause of immunosuppression in chickens that has been shown to affect influenza A virus (AIV) pathogenicity and shedding patterns. In this study, we compared the shedding of a low passage duck origin AIV in two groups of specific- pathogen free chickens: one group that was IBDV-inoculated and one sham-inoculated group. At three weeks age, the birds were divided and intranasally given one of three doses (102, 103, 105) of A/mallard/OH/421/1987 (H7N8) low pathogenicity AIV. Although the bursae of the IBDV-infected chicks had higher lesion scores, the damage was minimal. We observed that this low IBDV exposure increased resistance to AIV challenge. There was no difference between the mean days of shedding between the two groups, although the IBDV inoculated group shed less virus (approximately 1 log10 less per bird). Uninoculated contacts co-housed with the treatment groups given 105 AIV became infected. Additionally, chicks shedding approximately 103-4 EID50/ml AIV (CT of 33 or less) transmitted virus to cage mates. These findings demonstrate that the impacts of immunosuppressive agents on AIV challenge may vary, based on a number of confounding factors.
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8. Characterization of non-O1 and non-O139 Vibrio cholerae from seafood in Atlantic Canada.
PMID:日期:2026-06-09This study reports on a comprehensive genomic analysis of Vibrio cholerae strains isolated from shellfish grown in Atlantic Canada, specifically the non-choleragenic non-O1/non-O139 serogroups - NOVC, with the aim to provide a baseline in research of these bacteria and inform risk characterization to human exposure. Seafood homogenates were enriched using both standard saline and modified salt free protocols, for recovery of V. cholerae. Isolates were identified by MALDI TOF MS, and relatedness was assessed using composite correlation index (CCI) analysis and multilocus sequence typing (MLST). Genomic characterization through whole genome sequencing revealed that Canadian NOVC isolates cluster with environmental strains previously reported from coastal New Hampshire, USA, while CCI and MLST analyses confirmed that the isolates are closely related to each other, with several representing novel sequence types. Bioinformatic screening identified gene clusters encoding secretion systems, adherence factors, and accessory toxins, although cholera toxin genes (ctx) and the toxin coregulated pilus (tcp) were absent. Antimicrobial susceptibility testing indicated that all isolates were sensitive to the antibiotics tested and exhibited β hemolysis. In light of the evidence of increase in sea surface water temperature, these findings provide the first comprehensive genomic and phenotypic baseline of NOVC strains from Canadian shellfish and underscore the importance of continued surveillance as a potential increase in NOVC prevalence and human exposure risk.
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9. {"_":"Biofilm Disruption and Gene Expression Alteration by Phages against Multi-Drug-Resistant Pseudomonas aeruginosa.","i":[""]}
PMID:日期:2026-06-03Pseudomonas aeruginosa<i></i> is a major opportunistic pathogen responsible for severe human infections and is increasingly associated with multidrug resistance and limited treatment options. In this study, we isolated and characterized bacteriophages targeting drug-resistant P. aeruginosa<i></i> strains and evaluated their capacity to disrupt biofilms and modulate biofilm-associated gene expression. Phages recovered from sewage showed broad lytic activity against P. aeruginosa<i></i> and were classified morphologically into the families Podoviridae<i></i> and Myoviridae<i></i>. Two phages, A2 and A4, exhibited the highest lytic activity against the resistant strains examined. Both phages significantly degraded preformed biofilms, with phage A4 showing comparatively stronger antibiofilm activity. In addition, phage exposure altered the expression of several biofilm-associated genes, including pelA<i></i>, htpB<i></i>, bifA</i></i>, psl<i></i>, fimW<i></i>, and wspA<i></i>. A notable finding was the reversal of bifA<i></i> expression from downregulation at 12 h to upregulation at 24 h following treatment with phage A4. Collectively, these results demonstrate that phages A2 and A4 possess strong lytic and antibiofilm activities and are capable of modulating biofilm-associated transcriptional responses in drug-resistant P. aeruginosa<i></i>. The findings support the potential application of bacteriophages as alternative therapeutic agents against drug-resistant biofilm-associated infections.
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10. {"_":"Systematic characterization of four novel Twort-group bacteriophages infecting .","i":["Staphylococcus hyicus"]}
PMID:日期:2026-01-01Exudative epidermitis (EE) in piglets, caused by the bacterium , remains a major concern for the swine industry, particularly due to antimicrobial resistance. Bacteriophages represent a promising alternative treatment. This study aimed to isolate and characterize phages infecting from EE-affected swine farm environments in Quebec. Four novel phages (BQE1, BQE4, BQE7, BQL4) were isolated from water and manure samples. All produced clear plaques (<1 mm) and reached titers of 10-10 PFU/mL. Electron microscopy revealed myovirus morphology. Their genomes (115.4-134.5 kb) are closely related to the already described Twort phage but distinct from other phages. No virulence or antimicrobial resistance genes were detected in their genome. Host range analysis across 58 staphylococcal strains showed broad activity against (77%-80% susceptibility), whereas the Twort phage was largely ineffective (4%). Adsorption and growth assays indicated efficient infection, with latent periods of ∼50 min and burst sizes of 10-13 PFU/cell. Phages remained stable at 4 °C and room temperature but were less stable at 31 °C. Overall, these Twort-like phages display strong lytic activity and favorable genomic and biological characteristics, supporting their potential as targeted alternatives to antibiotics for controlling EE in swine production.