AUSTRALIAN JOURNAL OF CHEMISTRY澳大利亚化学杂志
AUSTRALIAN JOURNAL OF CHEMISTRY(英文缩写 AUST J CHEM),ISSN 0004-9425,eISSN 1445-0038,中文译名:澳大利亚化学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 1.224 | Q4 |
| 2022 | 1.100 | Q4 |
| 2023 | 1.000 | Q4 |
| 2024 | 0.900 | Q4 |
| 2025 | 1.100 | Q4 |
AUSTRALIAN JOURNAL OF CHEMISTRY 最新收录文献
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1. {"_":"The C-terminal region of granulin-1 promotes cell proliferation in the absence of regular secondary structure.","i":["Opisthorchis viverrini"]}
PMID:DOI:日期:2025-11-01Topical application of granulin-derived peptides has the potential to alleviate the burden of chronic wounds. Granulins are a family of growth factor proteins that generally contain six disulfide bonds and are found in most organisms. They have diverse sequences and complex structure-function relationships. Select examples are potent wound healing agents, making it important to improve our understanding of how granulins fold and the structural features critical for bioactivity. We have previously shown that fragments corresponding to the N-terminal region of the parasite granulin, -GRN-1, can fold autonomously even with a non-native disulfide bond and still have well-defined structures. Here, we show that this phenomenon appears unique to the N-terminal region, as a peptide corresponding to the C-terminal fragment of -GRN-1 does not fold independently in an analogous manner to the N-terminal region. Despite a lack of structure in the C-terminal granulin fragment, this peptide promotes cell proliferation, indicating that the sequence might be more important than the three-dimensional structure for -GRN-1 wound-healing bioactivity.
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2. Differential membrane binding of α/β-peptide foldamers: implications for cellular delivery and mitochondrial targeting.
PMID:DOI:日期:2023-08-01The intrinsic pathway of apoptosis is regulated by the Bcl-2 family of proteins. Inhibition of the anti-apoptotic members represents a strategy to induce apoptotic cell death in cancer cells. We have measured the membrane binding properties of a series of peptides, including modified α/β-peptides, designed to exhibit enhanced membrane permeability to allow cell entry and improved access for engagement of Bcl-2 family members. The peptide cargo is based on the pro-apoptotic protein Bim, which interacts with all anti-apoptotic proteins to initiate apoptosis. The α/β-peptides contained cyclic β-amino acid residues designed to increase their stability and membrane-permeability. Dual polarisation interferometry was used to study the binding of each peptide to two different model membrane systems designed to mimic either the plasma membrane or the outer mitochondrial membrane. The impact of each peptide on the model membrane structure was also investigated, and the results demonstrated that the modified peptides had increased affinity for the mitochondrial membrane and significantly altered the structure of the bilayer. The results also showed that the presence of an RRR motif significantly enhanced the ability of the peptides to bind to and insert into the mitochondrial membrane mimic, and provide insights into the role of selective membrane targeting of peptides.
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3. Determination of Sample Concentrations by PULCON NMR Spectroscopy.
PMID:DOI:日期:2022-01-01PULCON (Pulse Length Based Concentration Determination) is a powerful, versatile, non-invasive, and accurate technique for measuring solution concentrations during routine NMR spectroscopy. As solutes are quantified directly by their unique resonances, this technique avoids weight-based errors caused by contaminants (e.g. moisture), allows NMR samples to be directly employed in biological assays, and is particularly useful for quantifying small molecules, peptides, unstable molecules, and other materials that are difficult to weigh or handle. This article provides an introductory guide for biological and medicinal chemists, and highlights the diversity of applications.
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4. Efficient flow synthesis of human antimicrobial peptides.
PMID:DOI:日期:2020-04-01Organisms from all kingdoms of life have evolved a vast array of peptidic natural products to defend against microbes. These are known collectively as antimicrobial peptides (AMPs) or host defense peptides, reflecting their abilities to not only directly kill microbes, but also to modulate host immune responses. Despite decades of investigation, AMPs have yet to live up to their promise as lead therapeutics, a reality that reflects, in part, our incomplete understanding of these diverse agents in their various physiological contexts. Toward improving our understanding of AMP biology and the ways in which this can be best leveraged for therapeutic development, we are interested in large-scale comparisons of the antimicrobial and immunological activities of human AMPs, an undertaking that requires an efficient workflow for AMP synthesis and subsequent characterization. We describe here the application of flow chemistry and reverse phase flash chromatography to the generation of 43 AMPs, approaches that, when combined, significantly expedite synthesis and purification, potentially facilitating more systematic approaches to downstream testing and engineering.
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5. A theoretical assessment of structure determination of multi-span membrane proteins by oriented sample solid-state NMR spectroscopy.
PMID:DOI:日期:2020-01-01Oriented sample solid state NMR (OS-ssNMR) spectroscopy allows direct determination of the structure and topology of membrane proteins reconstituted into aligned lipid bilayers. While OS-ssNMR theoretically has no upper size limit, its application to multi-span membrane proteins has not been established since most studies have been restricted to single or dual span proteins and peptides. Here, we present a critical assessment of the application of this method to multi-span membrane proteins. We used molecular dynamics simulations to back-calculate [N-H] separated local field (SLF) spectra from a G protein-coupled receptor (GPCR) and show that fully resolved spectra can be obtained theoretically for a multi-span membrane protein with currently achievable resonance linewidths.
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6. On-resin strategy to label α-conotoxins: Cy5-RgIA, a potent α9α10 nicotinic acetylcholine receptor imaging probe.
PMID:DOI:日期:2020-01-01In-solution conjugation is the most commonly used strategy to label peptides and proteins with fluorophores. However, lack of site-specific control and high costs of fluorophores are recognised limitations of this approach. Here, we established facile access to grams of Cy5-COOH a two-step synthetic route, demonstrated that Cy5 is stable to HF treatment and therefore compatible with Boc-SPPS, and coupled Cy5 to the N-terminus of α-conotoxin RgIA while still attached to the resin. Folding of the two-disulfide containing Cy5-RgIA benefitted from the hydrophobic nature of Cy5 resulting in only the globular disulfide bond isomer. In contrast, wild-type α-RgIA folded into the inactive ribbon and bioactive globular isomer under the same conditions. Labelled α-RgIA retained its ability to inhibit acetylcholine(100 μM)-evoked current reversibly with an IC of 5.0 nM (Hill coefficient = 1.7) for α-RgIA and an IC of 1.6 (Hill coefficient = 1.2) for Cy5-RgIA at the α9α10 nicotinic acetylcholine receptors (nAChRs) heterologeously expressed in oocytes. Cy5-RgIA was then used to successfully visualise nAChRs in RAW264.7 mouse macrophage cell line. This work introduced not only a new and valuable nAChR probe, but also a new versatile synthetic strategy that facilitates production of milligram to gram quantities of fluorophore-labelled peptides at low cost, which is often required for experiments. The strategy is compatible with Boc- and Fmoc-chemistry, allows for site-specific labelling of free amines anywhere in the peptide sequence, and can also be used for the introduction of Cy3/Cy5 FRET pairs.
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7. The Mechanism of Membrane Permeabilization by Peptides: Still an Enigma.
PMID:DOI:日期:2019-01-01Peptide-induced permeabilization of lipid vesicles has been measured for decades and has provided many insights into the sequence-structure-function relationships of membrane-active peptides. However, researchers in the field have noted that many experiments show transient permeabilization, in which a burst of leakage occurs immediately after peptide addition, followed by a slowdown or cessation of leakage before all contents have been released. This widely observed, but rarely studied, phenomenon is not explained by standard equilibrium pore models that are commonly invoked in both experimental and computational studies. Here we discuss observations of transient permeabilization, and we outline a pathway towards understanding this enigmatic phenomenon.
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8. Synthetic Models for Nickel-Iron Hydrogenase Featuring Redox-Active Ligands.
PMID:DOI:日期:2017-05-01The nickel-iron hydrogenase enzymes efficiently and reversibly interconvert protons, electrons, and dihydrogen. These redox proteins feature iron-sulfur clusters that relay electrons to and from their active sites. Reported here are synthetic models for nickel-iron hydrogenase featuring redox-active auxiliaries that mimic the iron-sulfur cofactors. The complexes prepared are Ni(μ-H)Fe species of formula [(diphosphine)Ni(dithiolate)(μ-H)Fe(CO)(ferrocenylphosphine)] or NiFe complexes [(diphosphine)Ni(dithiolate)Fe(CO)(ferrocenylphosphine)] (diphosphine = PhP(CH)PPh or CyP(CH)PCy; dithiolate = S(CH)S; ferrocenylphosphine = diphenylphosphinoferrocene, diphenylphosphinomethyl(nonamethylferrocene) or 1,1'-bis(diphenylphosphino)ferrocene). The hydride species is a catalyst for hydrogen evolution, while the latter hydride-free complexes can exist in four redox states - a feature made possible by the incorporation of the ferrocenyl groups. Mixed-valent complexes of 1,1'-bis(diphenylphosphino)ferrocene have one of the phosphine groups unbound, with these species representing advanced structural models with both a redox-active moiety (the ferrocene group) and a potential proton relay (the free phosphine) proximal to a nickel-iron dithiolate.
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9. Total Solid-Phase Synthesis of Biologically Active Drosophila Insulin-Like Peptide 2 (DILP2).
PMID:DOI:日期:2017-01-01In the fruit fly , there are eight insulin-like peptides (DILPs) with DILPs 1-7 interacting with a sole insulin-like receptor tyrosine kinase (DInR) while DILP8 interacts with a single G protein-coupled receptor (GPCR), Lgr3. Loss-of-function mutation studies show that the neuropeptide DILP2 has a key role in carbohydrate and lipid metabolism as well as longevity and reproduction. A better understanding of the processes whereby DILP2 mediates its specific actions is required. Consequently we undertook to prepare DILP2 as part of a larger, detailed structure-function relationship study. Use of our well-established insulin-like peptide synthesis protocol that entails separate solid phase assembly of each of the A- and B-chains with selective cysteine S-protection followed by sequential S-deprotection and simultaneous disulfide bond formation produced DILP2 in good overall yield and high purity. The synthetic DILP2 was shown to induce significant DInR phosphorylation and downstream signalling, with it being more potent than human insulin. This peptide will be a valuable tool to provide further insights into its binding to the insulin receptor, the subsequent cell signalling and role in insect metabolism.
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10. Thienyl Difluoroboron β-Diketonates in Solution and Polylactide Media.
PMID:DOI:日期:2016-01-01Difluoroboron β-diketonates have impressive optical properties in both solution and the solid state. In particular, both fluorescence and room-temperature phosphorescence are present when the dyes are confined to a rigid matrix, such as poly(lactic acid) (PLA). To expand current knowledge and color range capabilities of this unique type of multi-emitting chromophore, a series of thienyl-substituted BFbdk complexes have been synthesized. The photophysical properties were investigated in methylene chloride solution and in the solid state as dye/PLA blends. By varying donor ability, i.e. methyl, phenyl, methoxyl, and thienyl substituents, and by changing the dye loading in the PLA media (0.1-10% dye loading) red-shifted emission was achieved, important for biological imaging applications. In dilute CHCl solution, complexes exhibited absorptions ranging from 350 - 420 nm, solid-state fluorescence in PLA ranging from 390 - 500 nm, and oxygen sensitive phosphorescence ranging from 540 - 585 nm in PLA blends. Promising candidates as dye/PLA blends serve as models for dyepolymer conjugates for application as biological oxygen nanoprobes.