{"_":" P2-4：一种针对 中病原体的潜在生物防治剂。","i":["变异马赛菌","铜绿假单胞菌","中华绒螯蟹"]}
{"_":" P2-4: a potential biocontrol agent against pathogenic in .","i":["Massilia varians","Pseudomonas aeruginosa","Eriocheir sinensis"]}
摘要(中文译文)
是一种临床上重要的细菌病原体，对水产养殖构成严重威胁。然而，关于分离株对抗水产养殖中病原体的信息有限。本研究以中华绒螯蟹致病性菌株为 prey 细菌，从水产养殖沉积物中分离到一株潜在捕食者——分离株 P2-4，并进一步表征了其基因组特征、抗菌相关基因、毒力基因、毒性代谢产物、致病性、抗菌谱以及对养殖环境中致病性菌株的体内外抗菌效果。分离株 P2-4 含有一条染色体和一个质粒，共有 75 个 tRNA、7 个 5S rRNA、7 个 16S rRNA、7 个 23S rRNA、34 个 sRNA、5,238 个编码基因、20 个基因组岛、1 个前噬菌体、23 个插入序列和 102 个重复序列。该菌株携带 19 个与细胞运动和细胞壁裂解相关的抗菌相关基因。此外，该分离株不携带毒力基因，不能产生溶血素、硫化氢、亚硝酸盐和氨，对实验动物无致病性，7 天急性 LD₅₀ 值高于 5.0 × 10⁵ CFU/mL。此外，该分离株除对目标病原体外，还对致病性菌株具有潜在抗菌活性。在浓度为 1.0 × 10⁴ 至 1.0 × 10⁷ CFU/mL 时，其对致病性菌株的抑菌率为 99.35% 至 99.99%；在剂量为 6.0 × 10⁵ 至 6.0 × 10⁷ CFU/g 饲料时，对感染实验动物的相对存活率为 42.31% 至 73.08%。据我们所知，本研究首次证明某菌株可作为水产养殖中对抗致病性菌株的潜在生物防治剂。
Abstract
is a clinically significant bacterial pathogen that poses a serious threat to aquaculture. However, there is limited information on isolates against pathogenic in aquaculture. In this study, a potential predator, isolate P2-4, was isolated from aquaculture sediment using Chinese mitten crab -pathogenic as the prey bacterium, and its genomic features, antibacterial-related genes, virulence genes, toxic metabolites, pathogenicity, antibacterial spectrum, and and antibacterial effects against pathogenic in were further characterized. Isolate P2-4 possessed one chromosome and one plasmid, with a total of 75 tRNAs, 7 5S rRNAs, 7 16S rRNAs, 7 23S rRNAs, 34 sRNAs, 5,238 coding genes, 20 genomic islands, 1 prophage, 23 insertion sequences, and 102 repeat sequences. It harbored 19 antibacterial-related genes (, , , , , , , , , , , , , , , , , , and ) associated with cellular motility and cell wall lysis. In addition, the isolate carried no virulence genes, was unable to produce hemolysin, hydrogen sulfide, nitrite, and ammonia, and was avirulent in with a 7-day acute LD value of above 5.0 × 10 CFU/mL. Furthermore, the isolate exerted potential antibacterial activity against pathogenic , , , and , besides . It exhibited bacteriostatic rates of 99.35 to 99.99% against pathogenic at concentrations of 1.0 × 10 to 1.0 × 10 CFU/mL, and displayed relative percentage survivals of 42.31 to 73.08% against infection in at doses of 6.0 × 10 to 6.0 × 10 CFU/g diet. To our knowledge, this study, for the first time, demonstrates a strain as a potential biocontrol agent against pathogenic in aquaculture.
如何引用
AMA
Yiyao Liu, Yueqi Yang, Meiling Liu, Sijia Chen, Haipeng Cao, Chunlei Gai, et al. {"_":" P2-4: a potential biocontrol agent against pathogenic in .","i":["Massilia varians","Pseudomonas aeruginosa","Eriocheir sinensis"]}. Frontiers in microbiology. 2026; doi:10.3389/fmicb.2026.1863139.
APA
Yiyao Liu, Yueqi Yang, Meiling Liu, Sijia Chen, Haipeng Cao, Chunlei Gai, et al (2026). {"_":" P2-4: a potential biocontrol agent against pathogenic in .","i":["Massilia varians","Pseudomonas aeruginosa","Eriocheir sinensis"]}. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2026.1863139
GB/T 7714
Yiyao Liu, Yueqi Yang, Meiling Liu, Sijia Chen, Haipeng Cao, Chunlei Gai, et al. {"_":" P2-4: a potential biocontrol agent against pathogenic in .","i":["Massilia varians","Pseudomonas aeruginosa","Eriocheir sinensis"]}[J]. Frontiers in microbiology, 2026 doi:10.3389/fmicb.2026.1863139.
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