使用激光散斑对比成像对人体皮肤微循环系统性微血管功能进行标准化评估的方案
Protocol for Standardized Assessment of Systemic Microvascular Function in Human Cutaneous Microcirculation Using Laser Speckle Contrast Imaging.
摘要(中文译文)
激光散斑对比成像（LSCI）是一种高分辨率、非侵入性的光学技术，能够实现微血管血流灌注的实时、全视野可视化。本方案提出了一套标准化方法，用于利用LSCI评估人体皮肤微循环的系统性微血管功能。由于该技术所获得的测量结果对环境及生理性混杂因素高度敏感，本方案强调严格的标准化流程，以提高可重复性和实验可靠性。本方案详细说明了必要的环境控制措施，包括室温稳定在23°C ± 1°C、受试者体位、适应过程以及图像采集过程中外部干扰的最小化。该方法将LSCI与两种互补的激发性操作相结合，以评估皮肤微血管反应性。采用闭塞后反应性充血评估综合微血管反应性，而采用离子导入驱动的药理学激发评估内皮功能。具体而言，给予乙酰胆碱以评估内皮依赖性血管舒张，给予硝普钠以评估内皮非依赖性血管舒张。本逐步可视化方案旨在促进标准化LSCI方法在临床和转化研究环境中的推广应用。该方法已成功应用于识别多种临床疾病中的微血管损伤，包括难治性高血压、糖尿病和冠状动脉疾病。通过实现微血管反应性的可重复且非侵入性评估，本方法为研究系统性血管健康、监测疾病进展以及评估针对微血管的干预措施疗效提供了有价值的工具。
Abstract
Laser speckle contrast imaging (LSCI) is a high-resolution, non-invasive optical technique that enables real-time, full-field visualization of microvascular blood perfusion. This protocol presents a standardized methodology for evaluating systemic microvascular function in human cutaneous microcirculation using LSCI. Because measurements obtained with this technique are highly sensitive to environmental and physiological confounders, the protocol emphasizes strict standardization procedures to improve reproducibility and experimental reliability. The protocol details essential environmental controls, including room temperature stabilization at 23°C ± 1°C, participant positioning, acclimatization procedures, and minimization of external interference during image acquisition. The methodology integrates LSCI with two complementary provocative maneuvers to evaluate cutaneous microvascular reactivity. Post-occlusive reactive hyperemia is used to assess integrated microvascular reactivity, whereas iontophoresis-driven pharmacological challenges are used to evaluate endothelial function. Specifically, Acetylcholine is administered to assess endothelium-dependent vasodilation, and sodium nitroprusside is administered to assess endothelium-independent vasodilation. This step-by-step visualized protocol is intended to facilitate the adoption of standardized LSCI methodologies in clinical and translational research settings. The approach has been successfully applied to identify microvascular impairment in several clinical conditions, including resistant hypertension, diabetes, and coronary artery disease. By enabling reproducible and non-invasive assessment of microvascular reactivity, this methodology provides a valuable tool for investigating systemic vascular health, monitoring disease progression, and evaluating the efficacy of interventions targeting the microvasculature.
如何引用
AMA
Ernesto Novaes, Andrea De Lorenzo, Eduardo Tibirica. Protocol for Standardized Assessment of Systemic Microvascular Function in Human Cutaneous Microcirculation Using Laser Speckle Contrast Imaging.. Journal of visualized experiments : JoVE. 2026; doi:10.3791/71634.
APA
Ernesto Novaes, Andrea De Lorenzo, Eduardo Tibirica (2026). Protocol for Standardized Assessment of Systemic Microvascular Function in Human Cutaneous Microcirculation Using Laser Speckle Contrast Imaging.. Journal of visualized experiments : JoVE. https://doi.org/10.3791/71634
GB/T 7714
Ernesto Novaes, Andrea De Lorenzo, Eduardo Tibirica. Protocol for Standardized Assessment of Systemic Microvascular Function in Human Cutaneous Microcirculation Using Laser Speckle Contrast Imaging.[J]. Journal of visualized experiments : JoVE, 2026 doi:10.3791/71634.
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