异氟烷辅助电针在大鼠少弱精子症模型中的作用
Isoflurane-Assisted Electroacupuncture in a Rat Model of Oligoasthenozoospermia.
摘要(中文译文)
少弱精子症是男性生殖功能障碍最常见的类型之一。在啮齿类动物研究中，在多次重复实验中一致地实施电针（EA）存在技术挑战，尤其是当联合腹部正中线与后肢穴位时，稳定的电极接触至关重要。选择中极（CV3）、关元（CV4）、足三里（ST36）和三阴交（SP6）是因为这些穴位常用于不孕症和精液异常的针刺方案，且腹部正中线加后肢的配置适合标准化的电极配对。本报告描述了一套实用方案，用于建立腺嘌呤诱导的大鼠少弱精子症模型，并在异氟烷辅助下实施CV3-CV4与ST36-SP6配对刺激的电针治疗。雄性Sprague-Dawley大鼠每日灌胃腺嘌呤28天以诱导生殖功能损伤。随后，在吸入麻醉下每日实施电针28天，使用3%异氟烷诱导、1.5%面罩维持，以实现稳定的针刺放置和电极配对（CV3-CV4和ST36-SP6），并设定明确的刺激参数（2 Hz，1.5 mA，30 min）。通过计算机辅助精液分析评估精子计数和活力，并辅以睾丸苏木精-伊红（HE）组织学检查来评价治疗效果。代表性结果表明，腺嘌呤暴露降低了精子计数和活力并破坏了生精小管结构，而电针与精液参数和睾丸形态的部分恢复相关。该方案为需要在少弱精子症大鼠模型中进行一致的电针实施及精液/组织学验证的研究提供了现成参考。
Abstract
Oligoasthenozoospermia is one of the most common forms of male reproductive dysfunction. In rodent studies, delivering electroacupuncture (EA) consistently across repeated sessions poses a technical challenge, particularly when combining abdominal midline and hind-limb acupoints, where stable electrode contact is critical. Zhongji (CV3), Guanyuan (CV4), Zusanli (ST36), and Sanyinjiao (SP6) were selected because these points are frequently used in acupuncture protocols for infertility and semen abnormalities, and the abdominal midline plus hind-limb configuration suits standardized electrode pairing. This report describes a practical protocol for establishing an adenine-induced rat model of oligoasthenozoospermia and for administering isoflurane-assisted EA with paired stimulation of CV3-CV4 and ST36-SP6. Male Sprague-Dawley rats received adenine by daily gavage for 28 days to induce reproductive impairment. Subsequently, EA was administered daily for 28 days under inhalational anesthesia, using 3% isoflurane for induction and 1.5% for mask maintenance, to enable stable needle placement and electrode pairing (CV3-CV4 and ST36-SP6) with defined stimulation parameters (2 Hz, 1.5 mA, 30 min). Treatment effects were evaluated via computer-assisted semen analysis for sperm count and motility, supplemented by testicular hematoxylin and eosin (HE) histology. Representative results indicate that adenine exposure reduced sperm count and motility and disrupted seminiferous tubule architecture, while EA was associated with partial recovery in both semen parameters and testicular morphology. This protocol offers a ready reference for studies requiring consistent EA delivery and semen/histological verification in a rat model of oligoasthenozoospermia.
如何引用
AMA
Qian Zhang, Xuanrui Chen, Chunxue Meng, Changbin Zhao, Qianxi Na, Jing Zhang, et al. Isoflurane-Assisted Electroacupuncture in a Rat Model of Oligoasthenozoospermia.. Journal of visualized experiments : JoVE. 2026; doi:10.3791/71878.
APA
Qian Zhang, Xuanrui Chen, Chunxue Meng, Changbin Zhao, Qianxi Na, Jing Zhang, et al (2026). Isoflurane-Assisted Electroacupuncture in a Rat Model of Oligoasthenozoospermia.. Journal of visualized experiments : JoVE. https://doi.org/10.3791/71878
GB/T 7714
Qian Zhang, Xuanrui Chen, Chunxue Meng, Changbin Zhao, Qianxi Na, Jing Zhang, et al. Isoflurane-Assisted Electroacupuncture in a Rat Model of Oligoasthenozoospermia.[J]. Journal of visualized experiments : JoVE, 2026 doi:10.3791/71878.
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