运动对心脏、肾脏和内皮功能障碍的蛋白水解相关标志物的影响
Influence of Exercise on Proteolysis-Related Markers of Cardiac, Renal, and Endothelial Dysfunction.
摘要(中文译文)
有氧体育活动对心血管、肾脏和内皮功能障碍相关的蛋白水解标志物（如不对称二甲基精氨酸（ADMA）和对称二甲基精氨酸（SDMA））的影响尚未得到充分研究。我们的目标是通过检测多种组织和不同性别间ADMA和SDMA的产生，来研究运动与蛋白水解相关标志物水平之间的相关性。方法：我们分析了体育活动分子传感器联盟（MoTrPAC）的公开数据，该数据包括先前收集的Fischer 344大鼠在渐进式跑台训练后的多组学分析结果。ADMA/SDMA的分析采用非靶向亲水相互作用液相色谱（HILIC）-正离子质谱法进行。通过RNA-seq检测骨骼肌（腓肠肌）、肾脏、肝脏、脂肪组织、血浆、心脏和小肠组织中ADMA/SDMA产生酶（蛋白质精氨酸甲基转移酶（PRMTs））及蛋白酶体相关基因的表达。在50或60只小鼠的队列中，分析了训练后一、二、四和八周的数据，并与久坐对照组进行比较。棕色脂肪中复合ADMA/SDMA水平在雌性中显著升高，在雄性中有一定程度升高。两种性别中小肠的ADMA和SDMA水平均下降，而雄性肾脏SDMA升高，雌性略有升高。SDMA在雌性骨骼肌中也出现短暂升高。RNA-seq揭示了蛋白酶体相关基因表达的性别特异性变化，包括雌性肾脏中的上调和雄性肝脏及脂肪组织中的下调。PRMT表达在雄性血液中下降，PRMT 1-3在雄性棕色脂肪中呈上升趋势。这些发现表明，运动诱导了复杂的组织和性别特异性的蛋白水解及甲基精氨酸调控模式，显示出与体育活动代谢益处相关的潜在关联。
Abstract
The effects of aerobic physical activity on proteolysis-related markers of cardiovascular, kidney, and endothelial dysfunction, such as asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), have not been well studied. Our goal was to investigate the correlation between exercise and the levels of proteolysis-related markers by examining the production of ADMA and SDMA across multiple tissues and between sexes. Methods: We analyzed publicly available data from the Molecular Transducers of Physical Activity Consortium (MoTrPAC), which included previously collected multi-omic profiling of Fischer 344 rats after progressive treadmill training. Analysis of ADMA/SDMA was performed using untargeted hydrophilic interaction liquid chromatography(HILIC)-positive mass spectrometry. Gene expression of ADMA/SDMA producers (protein arginine methyltransferases (PRMTs)) and proteasome-related genes was examined via RNA-seq in skeletal muscle (gastrocnemius), kidney, liver, adipose tissue, plasma, heart, and small intestine tissue. In cohorts of 50 or 60 mice, data were analyzed over one, two, four, and eight weeks post training and compared to sedentary controls. Composite ADMA/SDMA levels in brown adipose rose significantly in females and to some extent in males. ADMA and SDMA levels decreased in the small intestine in both sexes, while kidney SDMA rose in males and slightly in females. SDMA also increased transiently in female skeletal muscle. RNA-seq revealed sex-specific changes in proteasome-related gene expression, including upregulation in female kidney and downregulation in male liver and adipose tissue. PRMT expression decreased in male blood, and PRMT 1-3 trended upward in male brown adipose. These findings suggest that exercise induces complex tissue- and sex-specific patterns of proteolysis and methylarginine regulation, showing potential associations related to the metabolic benefits of physical activity.
如何引用
AMA
Tai G Metzger, Joon Sung, Paul Megee. Influence of Exercise on Proteolysis-Related Markers of Cardiac, Renal, and Endothelial Dysfunction.. Cureus. 2026; doi:10.7759/cureus.108088.
APA
Tai G Metzger, Joon Sung, Paul Megee (2026). Influence of Exercise on Proteolysis-Related Markers of Cardiac, Renal, and Endothelial Dysfunction.. Cureus. https://doi.org/10.7759/cureus.108088
GB/T 7714
Tai G Metzger, Joon Sung, Paul Megee. Influence of Exercise on Proteolysis-Related Markers of Cardiac, Renal, and Endothelial Dysfunction.[J]. Cureus, 2026 doi:10.7759/cureus.108088.
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