Sensors & Diagnostics传感器与诊断
Sensors & Diagnostics(英文缩写 SENS DIAGN),ISSN 2635-0998,eISSN 2635-0998,中文译名:传感器与诊断 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-10-01 至 2026-09-30，按本站收录文献的发表日期统计。
期刊介绍
Sensors & Diagnostics 最新收录文献
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1. Electrochemical DNA hairpin sensors for differentiating small molecule intercalation from minor groove binding.
PMID:日期:2026-09-23Small molecule double-stranded DNA intercalators have significant potential for therapeutic applications. However, screening for and confirming a drug candidate's intercalative behavior remains labor-intensive and costly. To address this, we investigated the sequence and biophysical parameters that affect the performance of electrochemical DNA hairpin sensors for streamlined identification of structural intercalators. These sensors utilize oligonucleotide (oligo) sequences that form hairpins upon intercalator binding. The 3' end of the oligo is modified with alkylthiol linkers for gold electrode surface monolayer self-assembly, while the 5' end carries a methylene blue redox reporter. Hairpin formation enhances electron transfer between methylene blue and the gold electrode, which can be detected voltammetry. We tested seven hairpin structures varying in stem length and sequence. Our optimal oligo, HP4, features a four-base-pair stem and responds to five DNA intercalators over a broad detection range, with EC in close agreement with published affinity ( ) values for these interactions. We further demonstrate HP4's ability to discriminate intercalator binding from a series of minor groove binders through significant differences in signal gain upon incubation. Altogether, our strategy establishes a platform for identifying intercalative compounds that should support the development of DNA-targeting therapeutics.
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2. DISCO-LAMP: a novel discontinuous LAMP assay for isothermal antigen detection.
PMID:日期:2026-07-28Proximity ligation assay (PLA) is a highly sensitive protein detection method in which DNA probes conjugated to affinity reagents are brought into proximity upon binding the same target molecule, thereby enabling greatly accelerated ligation of the DNA strands. The detection of the ligated DNA typically relies on PCR, which requires temperature cycling. In this study, we report on a novel discontinuous (DISCO)-LAMP assay that enables the wash-free detection of PLA products loop-mediated isothermal amplification (LAMP). Due to the exponential amplification nature of LAMP, a careful balance between efficient amplification of the ligated full-length DNA and minimal background amplification from the individual constituent DNA fragment is essential but often challenging to achieve. After extensive template/primer design and assay optimization, DISCO-LAMP assay achieved a detection limit of 1 fM for the full-length DNA while maintaining minimal background amplification at 10 nM of each individual DNA fragment. When functionalized with specific affinity reagents, DISCO-LAMP detected Shiga toxin 2 (Stx2) with a limit of detection (LoD) of 100 fM as well as both Wuhan-1 and Omicron spike protein using a newly engineered Designed Ankyrin Repeat Protein (DARPin) D targeting a conserved epitope on the SARS-CoV-2 spike protein. We believe DISCO-LAMP represents a versatile and efficient LAMP-based technology that is readily adaptable for sensing diverse targets PLA.
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3. Noninvasive malaria detection beyond blood sampling.
PMID:日期:2026-07-21Malaria remains a major global health challenge, particularly in sub-Saharan Africa, despite being one of the oldest documented human diseases. Although blood-based detection remains the mainstream diagnostic modality, its use is constrained by the need for trained personnel, sterile procedures, patient discomfort, and reduced sensitivity for low-parasitemia and asymptomatic infections. Recent advances in sensing and diagnostics, increasingly integrated with machine learning and artificial intelligence, have created new opportunities for noninvasive malaria detection using accessible biological samples and physiological signals. This critical review synthesizes recent progress in noninvasive malaria detection across nonblood sampling matrices, biophysical detection mechanisms, and data-driven analytical approaches. We examine diverse sampling matrices, including urine, saliva, exhaled breath, and skin-emitted volatile organic compounds with attention to their associated biomarkers, biological relevance, and operational feasibility. We further categorize detection technologies into three principal domains: biological sample-based platforms, including immunoassays, molecular amplification, biosensors, and mass spectrometry; acoustic, photoacoustic, and optical systems, including ultrasound, photoacoustics, optical imaging, and spectroscopy; and digital and connected health platforms, including wearable monitoring, smartphone imaging, and machine learning-enabled analysis. We then compare these modalities in terms of analytical performance, operational suitability, and translational potential for integration into healthcare systems. Finally, we present an implementation-oriented roadmap that highlights translational, equity, artificial intelligence, scalability, and regulatory considerations, identifying practical paths toward field-ready, affordable, and programmatically relevant noninvasive malaria detection.
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4. PEG-mediated viscous crowding controls dynamics and quantitative range in recombinase polymerase amplification nucleation site analysis.
PMID:日期:2026-07-07Quantitative nucleic acid amplification tests (NAATs), such as qPCR or digital PCR, are essential diagnostic tools in the fields of medicine, environmental monitoring, and food safety. Recent efforts to bring nucleic acid quantification to the point-of-need using rapid isothermal amplification assays struggle to provide quantitative precision and suitable quantitative range. Here we investigate a digital isothermal NAAT in a membrane-free microfluidic chip by systematically tuning recombinase polymerase amplification (RPA) chemistry with a polyethylene glycol crowding agent (PEG 35 000) that controls the dynamics of individual amplification nucleation site development. Amplification nucleation site analysis (ANSA) using real-time epifluorescence imaging reveals that individual nucleation sites exhibit Gaussian spatial intensity profiles whose amplitude, width, and total fluorescence evolve in time. Site diameter increases linearly during active amplification, while peak intensity follows an exponential growth curve before plateauing. Increasing PEG concentration results in smaller reaction site diameters and enables reliable discrimination of closely spaced amplification events with 8% PEG providing the best balance between amplification robustness and site separation. We observe quantitative detection from 10 to 10 HIV DNA copies per reaction over a 4 log window that fits a power-law model ( = 0.94). Compared with digital PCR, the chip requires no photolithography-based fabrication or droplet generators and is fabricated from low-cost laser-cut polymers and tape. By coupling viscosity-driven site formation with low magnification fluorescence imaging, PEG-tuned ANSA delivers rapid, calibration-free HIV viral load measurements that cover the clinical range for HIV management in a simple, low-cost membrane-free microfluidic chip.
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5. Translational opportunities in aptamer and nanobody lateral flow assays within the WHO REASSURED framework.
PMID:日期:2026-05-20The World Health Organization's REASSURED framework underscores the role of lateral flow assays (LFAs) in addressing diagnostic access gaps in low-resource regions affected by emerging and re-emerging pathogens, neglected tropical diseases, and diseases of unknown etiology. While antibody-based LFAs dominate clinical use, aptamers and nanobodies have emerged as alternative recognition elements with favorable stability, reproducibility, and manufacturing characteristics. This review examines the analytical performance of aptamer- and nanobody-based LFAs across infectious and immune-mediated disease contexts and situates these systems within the diagnostic development landscape. Across disease settings, analytically robust performance comparable to antibody-based formats is routinely demonstrated under controlled conditions. However, comparative analysis reveals a persistent disconnect between analytical feasibility and progression into standardized clinical validation and deployment. Translational outcomes are more often constrained by specimen access, comparator definition, manufacturing readiness, and regulatory precedent than by limitations in binder affinity or assay chemistry. These findings indicate that for researchers developing next-generation LFAs, analytical optimization alone is insufficient; successful translation increasingly depends on alignment with validation, manufacturing, and deployment frameworks that govern diagnostic evaluation.
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6. {"_":"Biophysical characterization of Cas9 binding and cleavage using real-time electronic biosensors.","i":["sp"]}
PMID:日期:2026-03-09CRISPR-Cas9 enables curative genome editing but requires precise control of target recognition, particularly when single-nucleotide polymorphisms (SNPs) influence specificity. Conventional biochemical and optical assays often rely on endpoint or ensemble-averaged measurements and therefore fail to resolve the real-time binding dynamics underlying off-target interactions. Here, we report a label-free, non-faradaic electrochemical impedance spectroscopy (nfEIS) platform that directly monitors Cas9-gRNA interactions on gold microelectrodes with single-base resolution at the sickle cell disease (SCD) locus. A guide RNA was designed to perfectly match the SCD mutation (A to T) while introducing a single PAM-proximal mismatch with the wild-type DNA (WD) sequence. Using 63-nucleotide synthetic DNA substrates representing SCD and WD targets, concentration-dependent binding assays were performed to extract equilibrium parameters. Hill-model analysis revealed higher affinity for the SCD target ( = 0.09 nM) relative to WD ( = 0.3 nM), confirming strong on-target binding and weakened interaction at the mismatch site. Magnesium dependence evaluation showed that 5 mM Mg enhanced discrimination by stabilizing on-target complexes while destabilizing mismatched binding, whereas at 1 mM Mg this selectivity was lost. Time-resolved kinetic measurements using 1 nM Cas9 and exponential fitting of the curve revealed rapid association ( = 1.85 min) and dissociation rates ( = 5.24 min) for SCD, consistent with efficient R-loop formation. In contrast, the WD target exhibited slower association ( = 2.68 min) and recurring transient binding with delayed dissociation ( = 34.38 min), corroborated by endpoint gel assays. Cas9 lacking gRNA showed only weak, unstable interactions. Overall, these results demonstrate that Cas9 specificity arises from both affinity differences and binding-residence dynamics. nfEIS thus provides a real-time, label-free platform for probing Cas9 fidelity, Mg-dependent activation, and gRNA design for therapeutic genome editing and diagnostics.
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7. Correction: High resolution voltammetric and field-effect transistor readout of carbon fiber microelectrode biosensors.
PMID:日期:2025-12-09[This corrects the article DOI: 10.1039/D2SD00023G.].
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8. Correction: Turn-on fluorescent sensors for Cu-rich amyloid β peptide aggregates.
PMID:日期:2025-12-09[This corrects the article DOI: 10.1039/D2SD00028H.].
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9. {"_":"A nucleic acid-based electrochemical detection method for sample analysis.","i":["post hoc"]}
PMID:日期:2025-11-17This work introduces a new electrochemical sensing approach, where the liquid sample containing nucleic acid targets can be blotted onto an electrode that is pre-functionalized with probe DNA. The post-hybridization signal and probe DNA signal (obtained by melting the hybrid) can be successively measured later, making the sensing scheme resilient to probe layer deterioration and circumventing the need to measure probe signal immediately before sample collection, ultimately mitigating the need for electrochemical sensing equipment at the sample collection site.
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10. Species-specific discrimination of bacterial biofilms using a ratiometric fluorescence sensor array and machine learning.
PMID:日期:2025-11-11Biofilms are intricate bacterial communities encased in a self-produced extracellular matrix (ECM) of DNA, lipids, proteins, and polysaccharides. The diverse ECM composition across bacterial species significantly influences the progression of biofilm-associated infections, making precise identification crucial for effective treatment. Traditional methods such as biochemical assays, MALDI-TOF mass spectrometry, DNA sequencing and culturing provide valuable insights but have notable drawbacks, including time-consuming procedures, high costs, and the need for specialized equipment and trained personnel. These limitations hinder the rapid and widespread adoption of biofilm identification in clinical settings, underscoring the need for more streamlined, accurate, and accessible methods. In this study, we employed a paper-based ratiometric sensor array with fluorescent dyes (3-hydroxyflavone derivatives) pre-adsorbed onto paper microzone plates to identify bacterial biofilms. The fluorescence signals from the sensor upon interaction with biofilms were analyzed using linear discriminant analysis and different machine learning algorithms, including neural networks, support vector machines, and naïve Bayes classifiers. Our results show that the sensor array accurately distinguishes between biofilms of eight species with 97.5% classification accuracy. It effectively identifies individual bacteria at OD as low as 0.002 o.u. Additionally, using neural networks, the sensor array achieves more than 95% accuracy in distinguishing planktonic bacteria from biofilms and shows over 85% accuracy in identifying clinical bacterial species and biofilms. These findings highlight the sensor's potential for high-precision biofilm identification in laboratory and clinical settings, offering a valuable tool for advancing biofilm research and enhancing clinical diagnostics.