Optica光学
Optica(英文缩写 OPTICA),ISSN 2334-2536,eISSN 2334-2536,中文译名:光学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 10.644 | Q1 |
| 2022 | 10.400 | Q1 |
| 2023 | 8.400 | Q1 |
| 2024 | 8.500 | Q1 |
| 2025 | 8.800 | Q1 |
Optica 最新收录文献
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1. Maskless and on-chip LED-array microscope with spatially varying angle calibration for centimeter-scale phase imaging.
PMID:日期:2026-02-20Conventional lens-based microscopes are constrained by a trade-off between resolution and field-of-view (FOV), which limits overall imaging throughput. Recent works have shown that on-chip imaging systems with LED-array-based illumination offer a cost-effective approach for large FOV phase imaging. However, this strategy faces two main challenges: (1) twin-image ambiguity can degrade phase reconstruction. While mask-based modulation can help, it adds system complexity due to fabrication and alignment requirements; and (2) the illumination angle from each LED varies across large FOVs and can degrade centimeter-scale phase reconstruction without calibration. Here, we present a computational framework to jointly achieve mask-free on-chip phase imaging and adaptive calibration of spatially varying illumination angles. The sensorFOVis divided into subregions, within each of whichLEDillumination is approximated as planar. LED illumination angles for each subregion are initialized geometrically. Phase retrieval is then performed within each subregion by constraining the reconstruction with a soft optical transparency prior while simultaneously refining angle estimates. Reconstructed phase maps are merged to produce a high-quality, large-FOV phase image. We demonstrate this approach by achieving centimeter-scale on-chip phase imaging (up to 2.7 × 1.7 cm) with micron-scale resolution across various biological tissue sections. This approach provides a simple, low-cost, and scalable solution for large-FOV and label-free imaging.
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2. Longitudinal, label-free, high-resolution imaging of glioblastoma spheroid response to therapy: a translational tool for preclinical evaluation of chemotherapy, radiation, and immunotherapy.
PMID:日期:2026-02-20Glioblastoma (GBM) is an aggressive brain tumor with limited treatment options and poor patient survival, underscoring the need for novel, to our knowledge, therapeutic strategies and improved preclinical models. Patient-derived tumor spheroids (PDTSs) offer a physiologically relevant platform for evaluating treatments such as chimeric antigen receptor (CAR) T cell therapy, chemotherapy, and radiation. However, significant challenges remain in monitoring the complex three-dimensional (3D) microenvironment of the GBM PDTSs. Current imaging techniques used for this purpose are primarily endpoint analyses which lack critical real-time, non-invasive capabilities that ultimately preclude longitudinal and continuous monitoring. In this study, we introduce quantitative oblique back-illumination microscopy (qOBM) as a label-free and non-invasive imaging approach for longitudinal and continuous, high-resolution monitoring of GBM PDTSs during treatment. qOBM enables real-time visualization of cellular processes, including apoptosis, cell migration, and T cell-mediated cytotoxicity by leveraging tomographic refractive index-based quantitative imaging. We construct a compact qOBM system that fits within common incubators and apply it to study the effects of radiation, chemotherapy, and immunotherapy on three patient-derived GBM cell lines, extracting both static and dynamic image features over a 72 h treatment period. Additionally, we develop machine learning models to predict spheroid viability and cytotoxicity, demonstrating the potential of qOBM to enhance treatment evaluation. Our findings establish qOBM as a powerful tool for longitudinal and continuous spheroid monitoring, offering a non-destructive, high-resolution alternative to conventional endpoint assays and improving the evaluation of preclinical treatments for GBM.
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3. Single-scan adaptive optics-enabled quantitative optical coherence tomography angiography for absolute three-dimensional retinal blood flow mapping.
PMID:日期:2026-01-16Accurate measurement of blood flow is critical for understanding metabolic function and disease progression, particularly in the retina, where conditions such as diabetic retinopathy, macular degeneration, and glaucoma are closely linked to impairment in microvascular circulation. However, current imaging techniques, including optical coherence tomography angiography (OCTA) and Doppler OCT, do not generally provide direct and absolute blood flow measurements, particularly at the capillary level. Adoption of adaptive optics (AO) and high-speed swept-source lasers in OCT systems has enabled video-rate volumetric acquisition with the ability to resolve individual red blood cells (RBC). Here, we present a quantitative AO-OCTA approach that enables 3D blood flow mapping by integrating OCTA-based vessel morphology with a 3D Radon transform of RBC streaks to measure cell velocity from the raw spatio-temporal OCT data. We describe a complete post-processing pipeline to determine single-cell velocity, vessel diameter, and flow rate using a single scan. We demonstrate depth-resolved flow rates across retinal vessels with diameters from 5 to 120 μm and velocities ranging from 0.5 to 54 mm/s, capturing the wide range of flow dynamics in the human retina. Our methodology is a powerful tool for quantitative blood flow imaging, establishing a robust method for noninvasive, high-resolution microvascular flow quantification with multiple potential applications in biomedical research and clinical diagnostics.
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4. Back-illumination interference tomography for imaging weak scattering in thick tissues.
PMID:日期:2025-12-20Biological tissues are composed of discrete compartments of biochemical media that often exhibit subtle differences in refractive index. Light propagating through these compartments partially diffracts in a forward direction with a phase shift. We introduce a microscopy technique to image this scattering signal in thick tissues, called back-illumination interference tomography (BIT). An incoherent source is demagnified and imaged past the focal plane of a high-numerical aperture objective lens, producing a small, semicoherent source of backscattered light. This backscattered light undergoes a phase inversion over the narrow depth of field of the microscope, providing interference contrast to weakly scattering objects at the focal plane. BIT offers a different source of contrast to conventional illumination and oblique back-illumination microscopy. Compared to these techniques, we show that BIT improves contrast to blood cells in microfluidic chambers and in a human capillary. Finally, we apply BIT to unstained, unlabeled bulk human tissue and compare side-by-side to adjacent frozen sections stained with hematoxylin and eosin. These results demonstrate the potential of BIT to provide high-resolution, high-speed, 3D imaging of unprocessed biological tissues.
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5. Ultrasensitive Alzheimer's disease biomarker detection with nanopillar photonic crystal biosensors.
PMID:日期:2025-10-20The recent development of drugs able to mitigate neurodegenerative diseases has created an urgent need for biomarker tests that can be readily used by practitioners. Although biomarker detection directly in patients' blood is now possible, low-cost point-of-care tests remain a challenge because relevant biomarkers, especially amyloid- ( ) peptides, are small, they occur at very low concentrations, and detecting a single marker is insufficient. Here, we demonstrate a photonic resonant sensor able to detect 0.2 pg/ml of and in 1% human blood serum, equivalent to 20 pg/ml in undiluted serum, which is the clinically required level. This high performance is achieved by combining gold nanoparticle amplification with a dielectric nanopillar photonic crystal structure in a dimer configuration, while also employing an immunoassay approach for high selectivity and specificity. The design combines high resonance Q-factor, amplitude, and sensitivity, ideally suited for sensing. We also show the detection of and peptides in the same channel, which is highly relevant for assessing disease progress and opens a route toward multiplexing. Together with the handheld operation we have demonstrated previously, these photonic innovations make a major contribution to the ability to detect and monitor the progression of neurodegenerative diseases such as Alzheimer's.
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6. SCREEN: SCatteREr ENabled optical asymmetry.
PMID:日期:2025-06-20Optics that allow us to see clearly along one viewing direction while obscuring others' view of us are useful in numerous settings, including privacy-preserving window screens and one-way mirrors for psychological studies. Additionally, due to the rise of cameras that are able to see outside the visible spectrum, there is a need for optics that can also provide one-way visibility at these wavelengths. This is particularly challenging for thermal (i.e., infrared) imaging because most existing methods require precise control of scene illumination, which is difficult to achieve in the infrared. To address this challenge, we demonstrate broadband, passive one-way visibility by precisely tuning the position and optical parameters of a single optical scatterer. We show the benefits of our approach in both a simulated and an experimental testbed. With experimental data, we demonstrate a 5.22× and 5.23× improvement in the degree of asymmetry for midwave infrared (MWIR) and visible (VIS) wavelengths, respectively. Ultimately, our method introduces a robust, passive one-way visibility system at midwave infrared (MWIR), which can aid in numerous privacy preservation applications.
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7. Space-time inverse-scattering of translation-based motion.
PMID:日期:2025-05-20In optical diffraction tomography (ODT), a sample's 3D refractive index (RI) is often reconstructed after illuminating it from multiple angles, with the assumption that the sample remains static throughout data collection. When the sample undergoes dynamic motion during this data-collection process, significant artifacts and distortions compromise the fidelity of the reconstructed images. In this study, we develop a space-time inverse-scattering technique for ODT that compensates for the translational motion of multiple-scattering samples during data collection. Our approach involves formulating a joint optimization problem to simultaneously estimate a scattering sample's translational position at each measurement and its motion-corrected 3D RI distribution. Experimental results with weak- and multiple-scattering samples demonstrate the technique's effectiveness, yielding reconstructions with reduced artifacts, enhanced spatial resolution, and improved quantitative accuracy for samples undergoing continuous translational motion during imaging.
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8. High-speed 4D fluorescence light field tomography of whole freely moving organisms.
PMID:日期:2025-05-20Volumetric fluorescence imaging techniques, such as confocal, multiphoton, light sheet, and light field microscopy, have become indispensable tools across a wide range of cellular, developmental, and neurobiological applications. However, it is difficult to scale such techniques to the large 3D fields of view (FOV), volume rates, and synchronicity requirements for high-resolution 4D imaging of freely behaving organisms. Here, we present reflective Fourier light field computed tomography (ReFLeCT), a high-speed volumetric fluorescence computational imaging technique. ReFLeCT synchronously captures entire tomograms of multiple unrestrained, unanesthetized model organisms across multi-millimeter 3D FOVs at 120 volumes per second. In particular, we applied ReFLeCT to reconstruct 4D videos of fluorescently labeled zebrafish and larvae, enabling us to study their heartbeat, fin and tail motion, gaze, jaw motion, and muscle contractions with nearly isotropic 3D resolution while they are freely moving. To our knowledge, as a novel approach for snapshot tomographic capture, ReFLeCT is a major advance toward bridging the gap between current volumetric fluorescence microscopy techniques and macroscopic behavioral imaging.
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9. Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.
PMID:日期:2025-02-20Wide-field (WF) imaging is pivotal for observing dynamic biological events. While WF chemical microscopy offers high molecular specificity, it lacks the sensitivity for single-molecule detection. In contrast, WF fluorescence microscopy provides live-cell dynamic mapping but fails to leverage the rich chemical information necessary for functional interpretations. To address these limitations, we introduce Wide-Field Bond-selective Fluorescence-detected Infrared-Excited (WF-BonFIRE) spectro-microscopy. This technique combines rationally optimized imaging speed and field-of-view (FOV) to achieve single-molecule sensitivity with bond-selective contrast. We demonstrate WF-BonFIRE's capabilities in imaging single molecules, cells, astrocytes, and live neurons, capturing single FOVs up to 50 μm × 50 μm, with further expansion via multi-FOV mosaicking. Additionally, we have implemented a new temporal-delay modulation scheme that allows real-time kilohertz WF-BonFIRE imaging with speeds up to 1500 Hz. We showcase the millisecond temporal resolution through monitoring the random motion of live Escherichia coli. Leveraging its ability to distinguish molecules through distinct narrow-band BonFIRE signals, we further demonstrate multicolor real-time tracking. WF-BonFIRE should significantly broaden the boundary for chemical imaging, enabling high-speed observations at unparalleled sensitivity levels.
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10. Enhanced biochemical sensing with high-Q transmission resonances in free-standing membrane metasurfaces.
PMID:日期:2025-02-20Optical metasurfaces provide novel solutions to label-free biochemical sensing by localizing light resonantly beyond the diffraction limit, thereby selectively enhancing light-matter interactions for improved analytical performance. However, high-Q resonances in metasurfaces are usually achieved in the reflection mode, which impedes metasurface integration into compact imaging systems. Here, we demonstrate a novel metasurface platform for advanced biochemical sensing based on the physics of the bound states in the continuum (BIC) and electromagnetically induced transparency (EIT) modes, which arise when two interfering resonances from a periodic pattern of tilted elliptic holes overlap both spectrally and spatially, creating a narrow transparency window in the mid-infrared spectrum. We experimentally measure these resonant peaks observed in transmission mode (Q~734 @ ~8.8 μ) in free-standing silicon membranes and confirm their tunability through geometric scaling. We also demonstrate the strong coupling of the BIC-EIT modes with a thinly coated PMMA film on the metasurface, characterized by a large Rabi splitting (32 cm) and biosensing of protein monolayers in transmission mode. Our new photonic platform can facilitate the integration of metasurface biochemical sensors into compact and monolithic optical systems while being compatible with scalable manufacturing, thereby clearing the way for on-site biochemical sensing in everyday applications.