TRENDS IN BIOTECHNOLOGY生物技术趋势
TRENDS IN BIOTECHNOLOGY(英文缩写 TRENDS BIOTECHNOL),ISSN 0167-7799,eISSN 1879-3096,中文译名:生物技术趋势 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 21.942 | Q1 |
| 2022 | 17.300 | Q1 |
| 2023 | 14.300 | Q1 |
| 2024 | 14.900 | Q1 |
| 2025 | 16.600 | Q1 |
TRENDS IN BIOTECHNOLOGY 最新收录文献
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1. Engineering Pseudomonas putida KT2440 for open-loop upcycling of mixed plastics.
PMID:日期:2026-09-24Current recycling technologies address less than 10% of global plastic waste, necessitating alternative valorization routes. Biological upcycling via enzymatic depolymerization combined with microbial conversion of the resulting monomers offers a promising pathway to transform mixed-plastic waste into valuable alternatives. In this research article, we employed a single engineered Pseudomonas putida KT2440 for simultaneous co-utilization of five plastic monomers including ethylene glycol, terephthalate, adipate, 1,4-butanediol, and l-lactic acid, which can be derived from enzymatic hydrolysis of polyethylene terephthalate (PET), poly(butylene adipate-co-terephthalate) (PBAT), polyester polyurethanes (PUs), and polylactic acid. Continuous fermentation over 21 days with alternating mixed-monomer feeds achieved steady-state growth and complete substrate depletion, yielding adaptive mutations that informed iterative strain improvement. Further engineering enabled the biosynthesis of (R)-3-hydroxybutyrate (R-3HB), and 0.70 g l R-3HB was produced directly from enzymatic hydrolysates of blended PET, PBAT, and thermoplastic PU. These results establish a viable bio-based approach for upcycling realistic mixed plastics into value-added bioproducts.
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2. Plasmid copy number control offers a versatile tool in synthetic biology applications.
PMID:日期:2026-09-23Plasmids are typically regarded as static delivery vehicles for foreign DNA. Here, we expose the pivotal role that plasmid copy number (PCN) control can play in synthetic biology, not only in Escherichia coli but also in the next-generation bacterial workhorse Vibrio natriegens. We show that the antibiotic selection marker can impact PCN, thus affecting growth and protein production, and that cells can be cotransformed with multiple variants of the same plasmid, with their PCN controlled simultaneously and in unison. We reveal that plasmid loss can be mitigated via the integration of an additional origin of replication (ori) and that PCN control can be leveraged to modulate horizontal gene transfer, which we illustrate within the context of conjugation-based intercellular communication. Finally, we expand the MoClo modular cloning framework with inducible PCN control for rapid prototyping and to enhance the performance of complex biocircuits.
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3. Nanozymes and the restoration of immune tolerance in autoimmune diseases.
PMID:日期:2026-09-23Autoimmune diseases (ADs) are characterized by persistent immune dysregulation and oxidative imbalance, yet current therapies largely rely on broad immunosuppression that rarely restores durable immune homeostasis. Growing evidence indicates that reactive oxygen species (ROS) function as both inflammatory mediators and essential immune regulators, limiting the effectiveness of indiscriminate ROS scavenging. Here, we highlight nanozymes as emerging catalytic therapeutics for (ADs) and redefine their role as regulators of redox-guided immune homeostasis. We propose a catalytic redox set-point framework in which nanozymes restore a physiological redox window that supports immune regulation while constraining pathological inflammation. This perspective establishes a unifying mechanistic link between nanozyme catalysis and immune restoration, and outlines future opportunities for tolerance-oriented design and clinical translation.
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4. Asking pragmatic and RAD questions about organoid intelligence.
PMID:日期:2026-09-22Debates about whether organoid intelligence systems are conscious, or can suffer, often stall on the question of what these organoid entities and systems are. A reflexive, anticipatory, and deliberative approach, grounded in a moral principle of complexity, instead asks what they do and provides guidance on that basis. Unanswerable questions are transformed into more workable processes.
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5. Designing artificial cofactors and their enzymes together.
PMID:日期:2026-09-19When replacing expensive redox cofactors, making biomimetic cofactors with desirable thermostability and redox properties is only half of the equation; enzymes must also be able to use them. Ji and colleagues confront that trade-off by pairing the development of a thermostable carbocyclic cofactor with a dedicated regeneration enzyme for sustained biocatalysis.
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6. Why cell agriculture struggles with meat and opportunities for non-food tissues.
PMID:日期:2026-09-17Cellular agriculture uses cells to produce animal-derived products without raising whole animals. However, scaling cultivated meat into affordable, structured food remains difficult. In this opinion, we argue that this difficulty reflects not isolated technical gaps but constraint coupling: the interdependence of tissue thickness, vascularization, cell-state stability, media cost, bioreactor scale, food safety, and consumer acceptance within the product goal of food-grade meat. We interpret recent diversification through this framework. Nonfood tissues and low-complexity cell-derived products do not eliminate these constraints but reconfigure them around product-specific requirements, including ingredient identity, material performance, durability, regulatory context, and commercial feasibility. This reframing positions diversification beyond meat as product-specific pathways with distinct constraint profiles.
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7. Liquid biopsy in glioblastoma: emerging technologies and translational opportunities.
PMID:日期:2026-09-16Glioblastoma remains highly aggressive, with limited treatment response and poor prognosis. Tissue biopsy (TB) is invasive and fail to capture tumor heterogeneity or temporal dynamics. Liquid biopsy (LB) provides a noninvasive alternative for real-time monitoring via circulating biomarkers, including cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), and extracellular vesicles (EVs) in plasma, cerebrospinal fluid (CSF), urine, and saliva. This review advances beyond prior biomarker catalogs by delivering a quantitative technology scorecard comparing cfDNA-, CTC-, and EV-based platforms in terms of sensitivity, clinical actionability, cost, and scalability. We introduce a multimodal decision matrix and an artificial intelligence (AI)-driven fusion pipeline integrating fragmentomics, EV proteomics, and CTC transcriptomics to enhance minimal residual disease detection and guide precision neuro-oncology.
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8. Enzyme evolution and metabolic engineering for 5-aminolevulinic acid production.
PMID:日期:2026-09-155-Aminolevulinic acid (5-ALA), a nonproteinogenic amino acid, has emerged as a versatile metabolite for applications in health, agriculture, and sustainability. Advances in ALA synthase engineering and metabolic rewiring have enabled scalable biosynthesis. The next frontier should expand beyond production, positioning it as a gateway to future biotechnological applications.
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9. Cross-target and cell-preferential CRISPR-Cas9 inhibition with carbohydrate-tagged oligonucleotides.
PMID:日期:2026-09-15Precise control of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) activity is important for limiting off-target effects and chromosomal rearrangements. Existing inhibitors, including anti-CRISPR proteins and spacer-targeting oligonucleotides, can be constrained by immunogenicity, target-sequence dependence, or delivery challenges. Here, we developed single-stranded DNA oligonucleotides that target conserved regions of the Streptococcus pyogenes Cas9 single-guide RNA (sgRNA) scaffold rather than the variable spacer. Screening identified single-stranded DNA 7 (ssDNA7), which targets stem-loop-1 and the adjacent linker and inhibits Cas9 activity across multiple tested spacer sequences and genomic loci without redesigning the inhibitor. Carbohydrate conjugation improved oligonucleotide stability and preferentially enhanced inhibitory activity in selected liver-derived or cancer cell models. Amplicon sequencing confirmed inhibition of endogenous editing in multiple cell models and normal liver-derived organoids, and supported cell-preferential inhibition in matched cell comparisons. These findings establish sgRNA-scaffold targeting as a strategy for cross-target inhibition of Cas9 and show that carbohydrate conjugation can tune its cellular activity.
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10. DipTRANS: an improved method for in planta transformation and genome engineering in Nicotiana benthamiana.
PMID:日期:2026-09-12Plant transformation remains constrained by labor-intensive tissue culture. Our previous work showed that direct delivery of developmental regulators (DRs) can induce de novo meristems on plants, offering a promising transformation approach. In this resource article, we introduced DipTRANS (Direct in planta Transformation), an optimized, soil-based heritable transformation platform for Nicotiana benthamiana that bypasses sterile culture entirely. DipTRANS is built on DR-induced de novo meristem formation. After optimizing parameters, including regulator combinations, Agrobacterium strain, and infiltration density, DipTRANS yielded transformation efficiencies to 46.7%. Developmental abnormalities associated with regulator expression are resolved through cutting-based propagation and virus-induced transgene excision, enabling recovery of fertile, transgenic progeny. Furthermore, DipTRANS supports tissue culture-free, transgene-free iterative genome modification via virus-induced genome editing. Overall, DipTRANS enables the generation of transgenic plants within 30 days and engineered progeny within 90 days. This methodology provides a rapid, versatile platform and a blueprint for extending direct in planta transformation to other plant species.