International Journal of Biological Macromolecules国际生物大分子杂志
International Journal of Biological Macromolecules(英文缩写 INT J BIOL MACROMOL),ISSN 0141-8130,eISSN 1879-0003,中文译名:国际生物大分子杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 8.025 | Q1 |
| 2022 | 8.200 | Q1 |
| 2023 | 7.700 | Q1 |
| 2024 | 8.500 | Q1 |
| 2025 | 8.700 | Q1 |
International Journal of Biological Macromolecules 最新收录文献
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2. Preliminary structural characterization and potential glucose metabolism-regulating effects of Armillaria mellea mycelial polysaccharide in insulin-resistant HepG2 cells.
PMID:日期:2026-09-24Armillaria mellea mycelial polysaccharide (AMMP) is a fungal bioactive macromolecule with potential metabolic regulatory activity; however, its preliminary structural characteristics and glucose metabolism-regulating effects in insulin-resistant hepatic cells remain inadequately clarified. In this study, AMMP was obtained by ultrasound-assisted enzymatic hydrolysis, purified through column chromatography, and preliminarily characterized using several physicochemical approaches. Its inhibitory effects on α-amylase and α-glucosidase were assessed as supporting in vitro evidence, and its regulatory activity in insulin-resistant HepG2 cells was further examined. The results showed that AMMP was a heteropolysaccharide fraction with a molecular weight of 15.6 kDa and was mainly composed of glucose (48.842%), galactosamine (14.647%), and mannose (13.564%). FT-IR and UV analyses revealed typical polysaccharide spectral characteristics, whereas one-dimensional H and C NMR spectra provided preliminary information on the general proton and carbon resonance regions. The relatively high proportion of galactosamine indicated the possible presence of amino sugar-containing components, although its exact origin and linkage pattern require further structural confirmation. AMMP exhibited moderate competitive and reversible inhibition of α-amylase and α-glucosidase, with weaker inhibitory potency than acarbose, and fluorescence quenching analysis suggested that the interactions were mainly mediated by van der Waals forces and hydrogen bonds. In insulin-resistant HepG2 cells, AMMP treatment (100-300 μg/mL) decreased glucose levels in the culture medium and cellular glycogen content, increased the activities of hexokinase, pyruvate kinase, and glucokinase, enhanced superoxide dismutase and glutathione peroxidase activities, and reduced malondialdehyde levels. In addition, AMMP regulated the mRNA expression of key genes in the PI3K/Akt signaling pathway. Transcriptomic analysis identified 32 DEGs under a stringent twofold-change criterion and 234 candidate DEGs under an exploratory threshold; among the latter, 35 remained statistically significant after Benjamini-Hochberg correction. GO enrichment analysis emphasized transcriptional regulation, metabolic and biosynthetic processes, oxidative stress, and metal ion-related responses, while PPI analysis identified IL6, JUN, and CXCL8 as the highest-degree candidate hub genes. KEGG analysis showed several exploratory pathway-level signals, although none remained significant after FDR correction. These findings provide preliminary in vitro evidence that AMMP may regulate glucose metabolism, oxidative stress, and insulin resistance-related molecular responses in insulin-resistant HepG2 cells, supporting further evaluation in suitable in vivo models.
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3. Genome-wide identification of Lhcb gene family and functional characterization of BcLhcb1.2 for cold tolerance in non-heading Chinese cabbage.
PMID:日期:2026-09-24The light-harvesting chlorophyll a/b binding protein (Lhcb) constitutes the antenna system of photosystem II (PSII), which also plays an important role in plant growth and responses to abiotic stress. However, the role of Lhcb genes in cold stress remains poorly characterized in non-heading Chinese cabbage. Here, 25 BcLhcb genes were identified and classified into 8 subfamilies, and these genes were mapped to nine chromosomes. Analysis indicated that BcLhcb genes may respond to hormonal and abiotic stress. Subcellular localization and expression pattern demonstrated that BcLhcb1.2, a chloroplast-localized gene, was significantly induced by cold stress. When BcLhcb1.2 was silenced, weaker cold tolerance was found than in control. And cold-responsive genes were more significantly down-regulated in BcLhcb1.2 silent lines. In overexpression assays, lower malondialdehyde content and higher proline content showed that BcLhcb1.2 enhanced cold tolerance of Arabidopsis thaliana, while lhcb1 weakened. Moreover, the abscisic acid sensitivity was higher in overexpressing lines and lower in the lhcb1 line, indicating that BcLhcb1.2 participated in the ABA signaling pathway. BcABF4, an ABA-responsive transcription factor, was identified which could bind the ABRE box in BcLhcb1.2 promoter and activate its expression. In summary, a novel regulatory model, ABA-BcABF4-BcLhcb1.2, was proposed to enhance cold tolerance. It provides new insights for resistance breeding in non-heading Chinese cabbage.
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4. Sustainable development of additively manufactured PLA composites reinforced with agro-waste derived bio-filler: Structural and flame-retardant performances.
PMID:日期:2026-09-24The increasing reliance on conventional composite materials has raised concerns regarding cost and environmental sustainability, particularly due to poor waste management. This study explores the potential of underutilized pineapple leaf fibre (PALF) agro-waste from North-Eastern India as a low-content natural reinforcement for PLA-based material-extrusion (MEX) 3D printing. Chemically treated PALF was incorporated at low concentrations (1-5 wt%) to develop lightweight bio-composites, with their surface, structural, chemical, mechanical, and flame-retardant characteristics systematically investigated. XRD analysis revealed an increase in crystallinity, while FTIR confirmed the absence of new chemical bonds, indicating predominantly physical interactions between PLA and PALF. The incorporation of PALF improved Shore D hardness, tensile strength, and compressive strength by 2.5%, 65.81%, and 7.61%, respectively, with the 95PLA/5PALF composite exhibiting the optimum overall performance. In contrast, flexural strength decreased relative to neat PLA. Flammability testing further demonstrated enhanced flame resistance, attributed to char formation and the resulting barrier effect, which reduced the burning rate. The developed PLA/PALF bio-composites provide a sustainable pathway for converting region-specific agricultural residues into functional 3D-printable materials with enhanced mechanical performance and flame resistance. The results also demonstrate their potential as lightweight, sustainable materials for structural and semi-structural applications while contributing to the development of a circular material economy.
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5. Mutagenesis-guided modeling of the Lynx1-α7 nicotinic acetylcholine receptor complex: An iterative feedback-driven approach.
PMID:日期:2026-09-24Human α7 nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel involved in many essential processes and considered important pharmacological target. Its function is regulated by GPI-anchored neuromodulator Lynx1. Despite huge progress in structural studies of α7-nAChR, there is still a lack of experimental data on its interaction with Lynx1. Here, we present an iterative modeling framework that combines exhaustive ensemble protein-protein docking with a quantitative two-sided mutagenesis-guided scoring. Water-soluble domain of human Lynx1 (ws-Lynx1) interacted with the resting state of human α7-nAChR and inhibited ACh-evoked currents through the receptor expressed in Xenopus oocytes with IC ~ 11 μM without affecting channel desensitization. Ws-Lynx1 and α7-nAChR residues selected from the initial docking model were replaced by alanine, and effects of their substitutions were evaluated and classified as Loss-of-Function, Gain-of-Function, or Neutral. Loss-of-Function substitutions were represented by ws-Lynx1[R37A] (IC ~ 60 μM) and α7-nAChR[K182A] (full activity loss). Based on mutagenesis data combined with a GPI-anchoring geometry restriction the initial number of docking solutions was reduced from 338,000 to seven. The top-scoring conformation was assembled into a pentameric (GPI-anchored Lynx1)-(N-glycosylated α7-nAChR) complex and refined by 1.5 μs of explicit-membrane unrestrained molecular dynamics. The refined model revealed several new features of the Lynx1-α7-nAChR interaction and suggests a mechanism underlying allosteric modulation. By this model, Lynx1 loop II and GPI-anchor form stable contacts with the ECD-TMD coupling region, preventing the receptor activation. Structural insights obtained here provide rational basis for the future design of new α7-nAChR modulators.
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6. Biodegradable antistatic starch foams via two-step extrusion: Linking additive effects, melt rheology, and cellular structure.
PMID:日期:2026-09-24Developing biodegradable starch foams with antistatic functionality remains challenging because antistatic additives can simultaneously influence charge dissipation, melt viscoelasticity, and foam-structure formation. Herein, a hierarchical film-to-foam strategy was developed to separate additive-specific effects from changes introduced during two-step extrusion foaming of starch-based materials. Homogeneous hydroxypropyl starch (HPS) films were first used to screen additives with strong antistatic potential and favorable interactions with the starch matrix, while pellet rheology was further applied to evaluate whether these additive effects could be translated into a melt state suitable for stable foaming. Polyvinylpyrrolidone (PVP) and 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) showed similarly strong antistatic potential in the film model, but generated different rheological responses in starch pellets. After foaming, high [EMIM][Ac] loading promoted expansion but weakened structural stability, whereas 1 wt% PVP provided the best overall balance, yielding low apparent density (21.26 kg·m), relatively high expansion ratio (32.94), reduced resistivity (724.62 MΩ·cm), and improved compressive resilience (140.41%) and recovery (58.46%). These results show that the performance of antistatic starch foams is governed not by additive chemistry alone, but by the sequential coupling of additive-starch interactions, melt-state regulation, and foam-structure development. This work provides a useful basis for designing multifunctional biodegradable polysaccharide foams with balanced electrical and mechanical performance.
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7. Gut microbiota modulation, short-chain fatty acid (SCFA) production, and immune responses associated with structurally distinct peach pectin fractions.
PMID:日期:2026-09-24Peach pectin is a promising functional polysaccharide, yet the structure-activity associations among its fractions remain poorly defined. Here, four structurally distinct peach pectin fractions, water-soluble (WSP), chelator-soluble (CSP), sodium carbonate-soluble (SSP), and an enzymatically modified fraction (ES) with targeted homogalacturonan fragmentation, were evaluated in cyclophosphamide-induced immunosuppressed female mice. Among them, SSP exerted the most comprehensive benefits. Specifically, SSP treatment restored cytokine balance; increased red blood cell count and hemoglobin levels by 24.56% and 34.39%, respectively, relative to the model group; enhanced microbial diversity (Shannon and Sobs indices increased by 21.17% and 12.05%, respectively); and optimized short-chain fatty acid profiles. These effects were strongly associated with SSP's structural traits, particularly moderate branching, higher rhamnogalacturonan I (RG-I) content, and characteristic monosaccharide composition. Correlation analysis identified taxon-specific responses, where norank_f_UCG-010 (unclassified genus) was correlated with the RG-I index (r = 0.99) and arabinose (r = 0.99), and Blautia, Enterorhabdus, and Eubacterium_brachy_group (unclassified genus) with rhamnose, fucose, and galactose, respectively. Higher branching was negatively correlated with Bacillus (r = -0.95) and norank_f_Desulfovibrionaceae (unclassified genus) (r = -0.99) and positively correlated with valeric acid levels (r = 0.96). Overall, structural differences of the peach pectin fractions were related to the differences in the modulation of the microbiota and the immune response in this model, highlighting SSP as a promising functional ingredient.
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8. RNA derived from cancer cells as a scaffold for amyloid-β25-35 aggregation: the effect of RNA methylation and rRNA status.
PMID:日期:2026-09-24Besides a well-established protein aggregation-mediated pathologies in neurodegenerative diseases, protein misfolding and subsequent accumulation of non-functional aggregates may also have a role during cancer progression promoting cancer cell survival and affecting drug responses. A number of factors may modulate the process of protein aggregation. However, the role of RNA as a scaffold and its methylation status were not elucidated yet. In the present study, we have used a cellular model of cancer, namely osteosarcoma (OS) cells to investigate RNA-mediated amyloid-β (Aβ) aggregation in the biological system not related to Aβ-mediated pathology. In the presence of Aβ25-35, RNA isolated from OS cells promoted the formation of protein aggregates in a concentration-dependent manner in vitro. Drug-induced senescent OS cells devoid of two mC RNA methyltransferases TRDMT1 and NSUN2 had limited ability to form protein aggregates. Nevertheless, this observation was associated with rRNA status and the expression of rRNA-related genes rather than RNA methylation. In contrast, RNA secreted by senescent wild type (WT) OS cells was characterized by increased mC levels compared to RNA intracellular pools that was accompanied by its ability to stimulate the formation of Aβ25-35 aggregates. In conclusion, we show for the first time that RNA derived from cancer cells may be a scaffold promoting the formation of Aβ25-35 aggregates. More studies are needed to validate the RNA-mediated protein aggregation in cellular systems and its modulation by RNA methylation and rRNA status. RNA amyloidogenic activity should also be assessed in the presence of full-length Aβ.
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9. Microbial transformation and cordycepin co-administration enhanced incretin-related and prebiotic potentials of galactomannans from two herbs.
PMID:日期:2026-09-24Regulation of gut microbiota composition by polysaccharides has been regarded as a potential therapeutic strategy. Accordingly, to evaluate the interaction between a specific type of polysaccharides (galactomannans) and intestinal bacteria and their in vitro activities, a total of twenty-three polysaccharides were isolated from two medicinal herbs rich in galactomannans or glucogalactomannans, Trigonella foenum-graecum and Cordyceps militaris. Two fractions from C. militaris,CM-1a02 and CF-1b03, exhibited significant effects on two important in vitro incretin-related anti-hyperglycemic targets (IC₅₀ = 75.0 μg/mL or 0.8 μM against DPP-4 and 2.4-fold in GLP-1 secretion, respectively), and promoted the growth of a representative gut microbe (Bacteroides ovatus, BO) for the digestion of galactomannans. In addition, TF-5, the main fraction from T. foenum-graecum, also showed similar bioactivity (2.1-fold in insulin secretion and IC₅₀ = 134.4 μg/mL or 4.0 μM against DPP-4). Molecular docking confirmed that TF_BO (the metabolite of TF-5 by BO degradation) binds to a DPP-4 catalytic pocket, providing structural insights into its mechanism of action. Notably, co-administration of CM-1a02 or CF-1b03 with cordycepin enhanced the inhibition of DPP-4. Chemical and spectroscopic identification of the four active fractions (TF-5, TF_BO, CM-1a02 and CF-1b03) resulted in the elucidation of their possible repeat units or partial fragments. These findings reveal the structure-function relationships between the results of the three incretin-based in vitro screenings and the interaction between a key gut microbe and the tested fractions.
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10. Substrate-pocket reshaping of an (R)-selective transaminase enables efficient asymmetric synthesis of (R)-3-amino-1-butanol.
PMID:日期:2026-09-24(R)-Selective ω-transaminases are valuable biocatalysts for the synthesis of chiral amines and pharmaceutical intermediates, but their practical application is often limited by insufficient catalytic efficiency, poor operational stability and unfavorable reaction equilibrium. Here, an Actinomycete-derived (R)-selective ω-transaminase, AcTA, was engineered for the asymmetric reductive amination of 4-hydroxy-2-butanone to produce (R)-3-amino-1-butanol. Through a combination of molecular modeling, alanine scanning, consensus analysis and iterative saturation mutagenesis, the substrate-binding pocket of AcTA was reshaped to improve substrate positioning and catalytic performance. The optimal triple variant M3 (H73R/A168V/S210R) exhibited a 13.74-fold increase in catalytic efficiency (k/K) compared with the wild-type enzyme. M3 also showed markedly enhanced thermostability, with T₅₀, t₁₂ and T values increased by 15.22 °C, 7.97 min and 6.46 °C, respectively. Under optimized reaction conditions, M3 converted 50 g/L 4-hydroxy-2-butanone with 94.28% conversion and 99.5% ee within 36 h. These results establish M3 as an efficient and thermostable biocatalyst for the asymmetric synthesis of (R)-3-amino-1-butanol and demonstrate that substrate-pocket reshaping is an effective strategy for improving (R)-selective transaminases.