BIOPROCESS AND BIOSYSTEMS ENGINEERING生物过程与生物系统工程
BIOPROCESS AND BIOSYSTEMS ENGINEERING(英文缩写 BIOPROC BIOSYST ENG),ISSN 1615-7591,eISSN 1615-7605,中文译名:生物过程与生物系统工程 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.434 | Q2 |
| 2022 | 3.800 | Q2 |
| 2023 | 3.500 | Q2 |
| 2024 | 3.600 | Q2 |
| 2025 | 4.300 | Q2 |
BIOPROCESS AND BIOSYSTEMS ENGINEERING 最新收录文献
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1. Application and progress of synthetic biology technology in amino acid production.
PMID:日期:2026-10-01Amino acids, as key bio-based chemicals, possess unique physiological functions and chemical properties, making them widely applicable in food additives, animal feed, pharmaceuticals, and cosmetics. However, conventional production methods suffer from inherent limitations, including low conversion efficiency, poor substrate utilization, and significant environmental burdens. The rapid advancement of synthetic biology has provided a revolutionary pathway for amino acid production through the systematic design and engineering of biological systems. This article reviews recent progress in synthetic biology for amino acid biosynthesis, covering the design and reconstruction of metabolic pathways, the development of genetic circuits and dynamic regulation technologies, and the establishment of high-throughput screening platforms. It further elaborates on production strategies for natural amino acids, branched-chain amino acids, and several non-canonical amino acids. On this basis, the key technical applications of synthetic biology in amino acid manufacturing are systematically expounded, and major bottlenecks-such as host cell adaptive evolution and metabolic flux balance-are discussed in the context of current synthetic biology strategies. By integrating and critically reviewing existing studies, this article aims to explore novel research and development strategies to advance the production of biosynthetic amino acids.
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2. High-solids ethanol production from waste paper through consolidated bioprocessing using advanced Saccharomyces cerevisiae strains.
PMID:日期:2026-10-01High-solids simultaneous saccharification and co-fermentation (SScF) of paper wastes were compared in lab-scale fed-batch (vertical) and batch (horizontal) bioreactor cultures. Fermentation of untreated label backing (LB), multilayer paper-based packaging (ML) and potato paper sacks (PS) paper wastes were optimised for solid loading to minimise the exogenous enzyme dosage with advanced consolidated bioprocessing (CBP) yeast strains, to achieve maximum technical performances in terms of ethanol concentration, yield and enzyme efficiency. Using cellulase-producing CBP yeast (Cellusec 2.0, 3.3 or 4.0) improved the enzyme efficiency by 1.5-fold at a final solid loading of 20% (w/w dry) compared to a higher solid loading of 30% (w/w) in fed-batch SScF. At 20% (w/w) solid loading and 2 FPU/g ds, enzyme efficiencies of 0.109, 0.078 and 0.067 g ethanol/FPU were recorded with corresponding ethanol titres of 59.3, 45.2 and 38.2 g/L and ethanol yields of 58.5, 43.9 and 57.1% of the theoretical maximum for LB, PS and ML, respectively. Whereas batch SScF at 30% (w/w) solid loading and 2 FPU/g ds achieved higher ethanol titres of 65.6, 50.7 and 54.3 g/L, the corresponding ethanol yields and enzyme efficiencies were markedly lower at 36.7, 28.0 and 52.1% of the theoretical maximum and 0.069, 0.050 and 0.062 g ethanol/FPU for LB, PS and ML, respectively. Fed-batch SScF in a vertical bioreactor at 20% (w/w) final solid loading was, therefore, the preferred configuration due to more favourable enzyme efficiencies, ethanol yields and enzyme cost.
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3. Biosynthesis of cyanophycin by high-cell-density cultivation of recombinant Escherichia coli for corrosion-resistant biomaterial applications.
PMID:日期:2026-10-01Cyanophycin granule polypeptide (CGP), also known as multi-L-arginyl-poly(aspartic acid), is a biodegradable biopolymer composed primarily of aspartic acid and arginine. Due to its versatile functional properties, CGP has attracted increasing interest for potential applications in food, medicine, cosmetics, agriculture, and corrosion inhibition. The objective of this study was to biosynthesize a biologically derived corrosion-inhibiting biomaterial using recombinant Escherichia coli BL21(DE3) expressing cyanophycin synthetase (CphA), followed by cost-effective induction strategy. Specifically, this work aimed to establish a high-cell-density cultivation process capable of achieving improved CGP production while reducing dependence on costly inducers such as IPTG. Initial shake-flask experiments demonstrated that lactose induction resulted in higher CGP production and biomass formation compared to IPTG induction. In addition, supplementation with phosphate, ribose, trace elements, yeast extract, and tryptone further improved CGP accumulation. Based on these findings, a high-cell-density fed-batch fermentation strategy using lactose as both inducer and carbon source was developed, maximum gravimetrically recovered crude soluble and insoluble CGP-containing fractions of 35.4 and 17.8 g/L. Product characterization was further supported by FTIR, XRD, MALDI-MS. Furthermore, the recovered CGP-related material was evaluated in preliminary corrosion inhibition experiments under acidic conditions and showed significant reduction in corrosion rates compared with untreated control samples, indicating its potential for future corrosion-protection applications.
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4. Valorization of fish oil byproduct methyl esters for omega‑3 polyunsaturated fatty acids production via immobilized CALB lipase‑catalyzed hydrolysis.
PMID:日期:2026-10-01Omega-3 polyunsaturated fatty acids (n-3 PUFAs) offer therapeutic benefits, including triglyceride reduction and management of cardiovascular and neurological disorders. This study investigates the use of fish oil processing by‑products, which consist mainly of fatty acid methyl and ethyl esters, as novel substrates for n‑3 PUFAs production. To overcome the low efficiency of conventional chemical methods and the vulnerability of free lipase to alcohol toxicity and poor reusability, we developed a stable immobilized lipase system. The target enzyme (CALB) was immobilized onto macroporous resin LXTE‑1000 via adsorption‑crosslinking, and optimal hydrolysis conditions were determined. The resulting immobilized enzyme (LXTE‑1000@CALB) showed markedly enhanced thermal and operational stability, retaining approximately 64.21% of its initial hydrolysis degree over ten consecutive batches. This work provides an efficient, green, and sustainable approach for valorizing fish oil by‑products and producing n‑3 PUFAs.
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5. Short-term aerobic pretreatment of swine manure enhances short-chain fatty acid production in anaerobic fermentation: performance and mechanisms.
PMID:日期:2026-10-01Facing the dual challenges of swine manure pollution control and carbon resource recovery under intensive farming conditions, conventional anaerobic fermentation technologies are limited in their application for high-value conversion due to low hydrolysis efficiency and high operational costs. To address these limitations, this study proposed a self-heating strategy using short-term aerobic pretreatment, aiming to optimize substrate pretreatment and enhance the synthesis of short-chain fatty acids during subsequent acidogenic fermentation. The results demonstrated that this pretreatment approach rapidly activates aerobic microbial metabolism and significantly improves the substrate's overall properties, as reflected by a decreased pH and increased conductivity, moisture content, total protein, and total organic carbon, and effectively promotes the solubilization and hydrolysis of organic matter, thereby increasing the bioavailability of substrates for anaerobic fermentation. Microbial community analysis further revealed that the pretreatment process selectively enriched hydrolytic-acidogenic bacteria, including Clostridium, Bacillus, Paenibacillus, and Streptomyces, while synergistically enhancing the activities of key metabolic enzymes such as cellulase, protease, and acetate kinase. These effects systematically strengthened the metabolic pathways from substrate degradation to acid synthesis. As a result, the short‑term aerobic pretreatment achieved a significant improvement in SCFAs production and energy efficiency. Specifically, the total SCFAs yield increased by 21.31% ± 4.23%, while energy consumption declined by 94.48% ± 1.48%. In addition, the unit acid production cost amounted to merely 4.37% ± 0.15% of that required for the conventional low‑temperature thermal treatment. Moreover, it significantly reduced the abundance of high-risk antibiotic resistance genes in fermentation residues, highlighting its environmental benefits. This study elucidates the synergistic mechanism of short-term aerobic pretreatment across "substrate-microorganism-enzyme" dimensions, confirming it as an efficient, economical, and sustainable pretreatment technology for swine manure resource recovery.
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6. Exploitation of Vibrio alginolyticus Mo245 as a dual producer of a glycosaminoglycan and poly(3-hydroxybutyrate) from glycerol.
PMID:日期:2026-10-01In the present work, the marine bacterium Vibrio alginolyticus Mo245 demonstrated the ability to secrete an extracellular polysaccharide (EPS) concomitant with the intracellular accumulation of a polyhydroxyalkanoate (PHA). Upon cultivation in glycerol as the sole carbon source, V. alginolyticus Mo245 co-produced a glycosaminoglycan (GAG), named EPS-Mo245, and the homopolymer poly(3-hydroxybutyrate) (P3HB), attaining maximum concentrations of 1.29±0.01 g/L and 1.78±0.00 g/L, respectively, within a 24 h cultivation. EPS-Mo245 was a hyaluronic acid (HA)-like GAG, composed of glucuronic acid (40.5±2.2 mol%), galactosamine (29.8±0.2 mol%), and glucosamine (21.1±0.6 mol%). It presented an average molecular weight (Mw) of 1945±67 kDa, with a polydispersity index (PDI) of 1.29±0.02, and a thermal degradation temperature of 235 °C. The produced P3HB had a Mw of 110±0.00 KDa and a PDI of 1.70±0.08, characterized by melting and thermal degradation temperatures of 175 °C and 293 °C, respectively, within the range of values reported for P3HB. These findings demonstrate V. alginolyticus Mo245 as a promising producer of both biopolymers, suitable for the valorization of glycerol, showcasing a sustainable route to yield two valuable biobased materials, an HA-like polysaccharide and P3HB, with potential for use in several applications.
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7. Streamlined stable CHO cell line development using droplet microfluidics with image-based monoclonality assessment.
PMID:日期:2026-10-01Rapid generation of monoclonal CHO cell lines remains a key bottleneck in mammalian cell line development (CLD), often requiring months of iterative screening and cloning. Here, we present an integrated droplet microfluidic workflow for high-throughput screening and isolation of antibody-producing ExpiCHO-S cells using a FRET-based secretion assay. The FRET assay was developed and optimized for picodroplet-based screening by evaluating probe configuration, donor-to-acceptor ratio, antibody concentration range, and signal saturation behavior. The platform enables picodroplet-based detection of secreted antibodies, sequential enrichment of antibody-producing cells, and image-verified single-cell isolation. Application of this approach yielded image-verified monoclonal antibody-producing clones that maintained consistent antibody production during the stability assessment performed in this study, including in the absence of selection pressure. By reducing the early clone-identification phase from DNA transfection to validated 96-well antibody-producing clones to approximately 5 weeks, this workflow addresses key limitations of conventional approaches and provides both a practical FRET assay-development framework and a scalable strategy for streamlining early CLD workflows in biomanufacturing.
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8. Modulation of mycelial formation in Streptomyces toxytricini and enhancement of lipstatin using light.
PMID:日期:2026-10-01Scanty reports exist on Streptomyces toxytricini, a natural producer of the anti-obesity pro-drug lipstatin. For the first time, a study has been conducted on the regulation of its secondary metabolite synthesis using light. The present study investigates the impact of multiple light conditions on mycelial hyphal development and lipstatin production in S. toxytricini. Upon examining the mycelial, hyphal, and pellet morphogenesis under various light conditions, the differential pattern from dense pellet to loose mycelial hyphae was observed in the submerged fermentation condition. Biomass accumulation, as indicated by dry cell weight (DCW), showed a significant increase under red (4.2 g/L), indigo (4 g/L), green (4.13 g/L), yellow (3.37 g/L), and violet light (3.53 g/L) conditions. The maximum biomass was observed under red light, showing a four-fold increase compared with the dark condition (1 g/L). Along with the biomass and mycelial arrangements, red light produced significantly smaller pellet sizes (81.4 ± 13 μm) than the dark (784.7 ± 46 μm) and white-light (332.5 ± 59 μm) conditions. The HPLC analysis confirmed the significant effect of the red-light condition on lipstatin yield, with ~ 6 g/L, five-fold higher than ~ 1.2 g/L in white light and 300-fold higher than ~ 0.02 g/L in dark conditions. Besides that, in silico analysis of the photoreceptors identified two putative bacteriophytochrome (BphP) containing proteins in S. toxytricini, suggesting a potential sensory framework of red-light perception. Collectively, this work highlights light as a promising and easily implementable process parameter for enhancing secondary metabolite production, providing a foundation for future mechanistic and omics-based investigations.
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9. Application of dielectric spectroscopy combined with simple linear and multivariate modelling for in-line monitoring of biomass concentration in E. coli cultures.
PMID:日期:2026-10-01Biomass concentration is a crucial parameter while monitoring Escherichia coli bioprocesses. Real-time monitoring of this attribute is preferable to discrete measurement, as it immediately detects deviations, reduces sampling efforts and thereby minimizes the risk of contamination. Therefore, the potential of dielectric spectroscopy for continuous monitoring of E. coli cultures was investigated. Two datasets were acquired across culture batches in a benchtop bioreactor: ∆ permittivity and the dielectric spectrum. For each batch, variations in ∆ permittivity followed the OD trend, highlighting the E. coli growth phases. The dielectric spectra also evolved throughout the batches, and Principal Component Analysis (PCA) revealed at least two main contributors to spectral variability. Both datasets were then used to estimate the OD using either simple linear or multivariate Partial Least Squares (PLS) modelling, with the aim of comparing these two strategies. For four calibration batches, both simple linear and PLS models generally predicted the OD accurately, with only minor differences. For a validation batch, both strategies showed good predictions during exponential growth but were slightly less accurate in the other bacterial culture phases. Prediction noise was also evaluated, and PLS provided slightly less noisy predictions than the simple linear model. Overall, this indicates that simple linear and PLS predictions are not considerably different, and both modelling strategies can be used indistinguishably to calibrate the dielectric signals for estimating the OD in E. coli cultures.
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10. Raman-guided sample subset selection for cost-efficient offline calibration in bioprocesses.
PMID:日期:2026-10-01In bioprocess engineering, model-based methods play a vital role in understanding complex dynamics of novel species or strains. However, the model development is often hampered by prohibitive costs associated with redundant reference analyses and poorly informed sampling schedules due to insufficient prior knowledge about the process dynamics. We propose a model-free PAT strategy, termed Raman-guided sample subset selection (RGSS) to prioritise informative offline reference assays from inline Raman spectra before nonlinear model calibration. RGSS is demonstrated with Saccharomyces cerevisiae fed-batch fermentations and evaluated by auto- and cross-validation (CV), as well as with practical identifiability analysis of an unstructured mechanistic model. The approach is implemented using constrained vector quantization (CVQ) with full-spectrum and analyte-specific wavenumber-selection and benchmarked against uniform-in-time sampling, random subsampling, and Kennard-Stone (KS) selection. The best RGSS scenarios, using only five selected reference assays per analyte, retained offline CV accuracy (NRMSE = 3.49) close to the full-data reference (NRMSE = 3.29) using all 22 samples. KS selection was also competitive for ten selected samples (NRMSE = 3.79), whereas uniform-in-time sampling resulted in higher CV errors for five and ten samples (8.47 and 65.57). Random subsampling occasionally produced competitive subsets, but showed broad variability over 20 random runs (NRMSE median [IQR]: 6.14 [4.84-47.76] and 15.48 [5.07-44.34]) for five and ten randomly selected samples. These results support RGSS as a cost-efficient, spectrally informed sample subset selection framework for prioritising offline assays in model-based bioprocess development.