FEMS MICROBIOLOGY LETTERSFEMS微生物学快报
FEMS MICROBIOLOGY LETTERS(英文缩写 FEMS MICROBIOL LETT),ISSN 0378-1097,eISSN 1574-6968,中文译名:FEMS微生物学快报 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.820 | Q3 |
| 2022 | 2.100 | Q4 |
| 2023 | 2.200 | Q3 |
| 2024 | 2.200 | Q3 |
| 2025 | 2.000 | Q4 |
FEMS MICROBIOLOGY LETTERS 最新收录文献
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1. Lineage-specific remodeling of a conserved tRNA-Thr-associated genomic island in Edwardsiella anguillarum.
1. 鳗爱德华氏菌中一个保守的tRNA-Thr相关基因组岛的谱系特异性重塑PMID:日期:2026-10-01Edwardsiella anguillarum is a bacterial pathogen affecting diverse aquaculture species worldwide, including red seabream, Japanese eel, and tilapia. In Japan, strains show a phenotypic dichotomy, where red seabream-derived strains are non-motile and ornithine decarboxylase (ODC)-negative, whereas eel-derived strains retain both traits. However, the evolutionary processes underlying lineage diversification within this species remain poorly understood. We performed a global comparative genomic analysis of 24 E. anguillarum strains, including seven newly sequenced genomes from Japan. Despite high ANI values (>99.6%), SNP analysis revealed lineage-level divergence, with 6 500-7 500 SNPs separating red seabream-associated and eel-associated lineages. Comparative analysis identified a conserved chromosomal hotspot adjacent to the tRNA-Thr gene with lineage-specific structural variation. The Japanese red seabream-associated lineage possessed a compact ∼10-kb region containing a Type I-E CRISPR/Cas system, whereas eel-associated and tilapia-derived strains carried expanded genomic islands (∼48-52 kb) enriched in DNA repair and mobile genetic element functions. These configurations were associated with phylogeographic lineages. Genes associated with motility and ODC activity were conserved across lineages, with no evidence of gene loss or major structural disruption. Phylogenetic analysis of the island-associated tyrosine-type recombinase/integrase (YR) was broadly consistent with the core genome. A shared frameshift mutation in eel- and tilapia-derived strains was consistent with a shared pseudogenization event. Overall, our findings indicate that diversification in E. anguillarum is associated with localized structural variation at a conserved tRNA-Thr-associated chromosomal hotspot within an otherwise highly conserved core genome.
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2. Genomic characterization of clinical Burkholderia semiarida SYBS718, a Burkholderia cepacia complex strain isolated from an immunocompromised patient.
PMID:日期:2026-09-30Burkholderia semiarida, a plant-pathogenic species within the Burkholderia cepacia complex (Bcc), has emerged as a worrying opportunistic pathogen causing serious respiratory infections in patients with cystic fibrosis (CF). In this study, strain SYBS718 was isolated from the blood and sputum of an immunocompromised patient. Conventional MALDI-TOF mass spectrometry and 16S rRNA sequencing misidentified this isolate as Burkholderia cenocepacia, whereas whole-genome sequencing enabled accurate species confirmation. Genomic analysis predicted a total of 569 virulence factors, 11 antibiotic resistance genes, and 1112 type III secretion system effector proteins related genes through the core databases of VFDB, CARD, and TS33. The virulence factors mainly include type VI secretion system (T6SS), type III secretion system (T3SS), iron uptake system, flagella, and type IV pili (TFP), which are associated with bacterial colonization, invasion, and pathogenicity. And the antibiotic resistance genes are primarily associated with fluoroquinolone, tetracycline, and aminoglycoside antibiotics. This study is the first to perform comprehensive genomic characterization of a clinical B. semiarida isolate. Our findings reveal the limitations of routine Bcc identification, and provide fundamental genomic data for further clinical and mechanistic research on this emerging opportunistic pathogen.
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3. Molecular mechanism of postharvest quality deterioration in Stropharia rugosoannulata based on transcriptomics and metabolomics.
3. 基于转录组学和代谢组学的酒红球盖菇采后品质劣变分子机制研究PMID:日期:2026-09-30Stropharia rugosoannulata is a major commercial mushroom species. After harvest, the mushroom is prone to rapid quality deterioration, such as browning, water loss, and softening, thereby significantly limiting its shelf life and commercial value. This study utilized cap tissues at three storage stages (immediately postharvest (0 d), 7 days postharvest, and 14 days postharvest, stored at 15°C) for transcriptome sequencing and untargeted metabolome analysis with integrated data analysis. Comparative transcriptome analysis identified 2,955 differentially expressed genes exhibiting consistent upregulation or downregulation trends during postharvest storage. Metabolome analysis screened 43 non-redundant differentially abundant metabolites. Joint KEGG pathway mapping of differentially expressed genes and differentially abundant metabolites identified several metabolic pathways significantly enriched at both transcriptome and metabolome levels, including carbohydrate metabolism, cell wall polysaccharide degradation, and reactive oxygen species metabolism. Within these pathways, expression trends of key enzyme-encoding genes demonstrated significant consistency with changes in abundances of corresponding substrates, key intermediates, and energy molecules. This study provides important data for elucidating postharvest stress responses and enzymatic regulatory mechanisms of S. rugosoannulata, offering potential molecular targets and theoretical support for developing postharvest preservation technology systems.
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4. Transcriptional analysis of D, D-carboxypeptidase genes as secondary cephalosporin resistance reporters in Escherichia coli harbouring blaCTX-M.
4. 携带blaCTX-M的大肠杆菌中D,D-羧肽酶基因作为次要头孢菌素耐药报告基因的转录分析PMID:日期:2026-09-30The emergence of novel plasmid-encoded resistance variants complicates antimicrobial resistance detection by conventional PCR-based methods. Therefore, endogenous secondary resistance genes expressed under antibiotic stresses to produce adaptive resistance mechanisms in bacteria may serve as reporters for AMR detection. This study investigated the transcriptional expression of D,D-carboxypeptidase genes and the transcriptional regulator bolA under oxyimino cephalosporin stress. Thirty clinical Escherichia coli isolates were tested for antibiotic susceptibility and extended-spectrum β-lactamase genes. Network analysis was performed using Cytoscape. D,D-carboxypeptidase genes were transformed into E. coli HST08 and B. subtilis RIK 1285 using pBE-S DNA; while blaCTX-M-harbouring plasmid was transformed into E. coli DH5α and subsequently eliminated from the parent isolate. MIC determination, growth assessment, and qRT-PCR were performed under ampicillin and oxyimino cephalosporins stress. Network analysis linked peptidoglycan synthesis, β-lactam resistance, and metabolic pathways with penicillin binding protein (PBP)-forming gene sets. Among 27 resistant isolates, 20 harboured blaCTX-M and 15 blaTEM. B. subtilis dacD and E. coli dacA clones showed elevated MICs. The study revealed consistent upregulation of dacB and dacD with host-specific effects on MICs, highlighting their potential as reporters for cephalosporin resistance in future diagnostics. Differential transcriptional responses observed among recombinant strains further suggested host-specific adaptive mechanisms during antibiotic exposure.
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5. Known QAC determinants outperform genome language model embeddings in a leakage-aware public Listeria benzalkonium chloride tolerance benchmark.
5. 在考虑数据泄漏的公开李斯特菌苯扎氯铵耐受性基准测试中，已知的QAC决定因素优于基因组语言模型嵌入PMID:日期:2026-09-26Whole-genome sequencing and pretrained DNA models may help prioritize disinfectant-tolerance hypotheses, but phenotype prediction requires measured, isolate-linked labels and evaluation that keeps related lineages apart. We compared conventional sequence features, numerical DNA representations generated by DNABERT2, and known quaternary ammonium compound (QAC) determinants in a public benchmark of 197 Listeria monocytogenes isolates with benzalkonium chloride MIC data. Models were evaluated across five repeated lineage-grouped train, development, and test splits. The known-QAC determinant rule showed the highest balanced accuracy (mean ± SD, 0.961 ± 0.057) and ROC AUC (0.961 ± 0.057). A model combining k-mer, DNABERT2, and QAC scores reached balanced accuracy 0.915 ± 0.023, whereas the best DNABERT2-based classifier reached 0.570 ± 0.110; sparse k-mer models did not exceed the dummy baseline. Public non-Listeria studies lacked the row-level phenotype-genome linkage required for supervised modelling. An exploratory bioinformatic screen therefore identified only candidate regions for future testing, not validated tolerance predictions. Because the benchmark contained only 197 publicly linkable isolates, these findings are hypothesis-generating and require confirmation in larger, independent phenotype-linked cohorts. Within these limits, interpretable determinants were the primary validated signal and DNA-model scores were secondary evidence.
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6. Mechanisms of yield improvement in dryland wheat under plastic film mulching: Insights from soil fungal communities and function.
6. 地膜覆盖下旱地小麦增产机制：来自土壤真菌群落与功能的启示PMID:日期:2026-09-24Plastic film (PF) mulching is an important method for winter wheat cultivation. In this study, the effects of PF mulching on wheat yield were analysed. Compared with non-mulched control, grain yield increased by 70% in PF treatment. Soil available phosphorus (AP) and electrical conductivity (EC) increased by 104.70% and 5.55%, respectively. PF mulching was associated with shifts in the relative abundance of several fungal taxa, including increases in Talaromyces, Trichoderma, and Schizothecium. The overall abundance of taxa classified as potential plant pathogens decreased by 37.73%. However, some taxa that include known plant pathogens, such as Didymella and Bipolaris, showed increased abundance, suggesting the need for careful disease management under mulching conditions. Co-occurrence network analysis indicated that fungal interactions became less complex in PF treatment, accompanied by an increased proportion of positive correlations. Redundancy analysis showed that soil EC and AP were significantly associated with wheat yield and with the relative abundance of specific fungal taxa. PF mulching was associated with changes in soil properties and fungal community structure, which may contribute to improved wheat yield. It provides insights into the potential role of fungal communities in mulching systems and highlights the importance of considering pathogen risks in wheat production.
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7. A comprehensive report on the 1st Swiss-EUCAST Antimicrobial Susceptibility Testing (AST) Meeting.
7. 关于首届瑞士-欧盟CAST抗菌药物敏感性测试（AST）会议的全面报告PMID:日期:2026-09-23Antimicrobial susceptibility testing (AST) is being reshaped by increasingly complex resistance mechanisms, revised interpretive frameworks, and rapid phenotypic and genomic technologies. The first Swiss-EUCAST AST Meeting, held in Zurich on 4 May 2026, brought together more than 100 clinical microbiologists, researchers, and infectious diseases specialists to discuss how these developments should be translated into routine diagnostics. The meeting focused on three topics: the limitations of genotype-phenotype prediction, the ongoing need for standardized interpretation within the European Committee on Antimicrobial Susceptibility Testing (EUCAST), and the potential of new technologies to reduce turnaround times. Discussions specifically on Pseudomonas aeruginosa, as well as on pathogens without clinical breakpoints, carbapenemase-producing Enterobacterales, expected phenotypes, epidemiological cutoff values, rapid phenotypic susceptibility tests, and whole-genome sequencing, showed that no single method can eliminate all diagnostic uncertainties. In summary, it was concluded that standardized phenotypic ASTs will continue to serve as a clinical reference in the future, but molecular and genomic methods offer additional advantages in terms of speed, as well as insights into mechanisms of action and epidemiological relations.
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8. Fungistatic Potential and Inhibition of Fungal Yeast-hyphae switch of Candida spp. by Hecogenin Acetate.
8. 醋酸海柯皂苷元对念珠菌属的抑真菌潜能及对真菌酵母-菌丝转换的抑制作用PMID:日期:2026-09-23Invasive fungal infections represent one of the leading causes of morbidity and mortality in immunocompromised patients, and many of the currently available antifungal agents are associated with toxicity, recurrence, or the development of resistance. Plants are a promising source for the discovery of new antifungal compounds due to their vast chemical diversity, among which saponins are widely distributed metabolites. Hecogenin acetate is a sapogenin-the hydrophobic moiety of saponins-and has demonstrated antioxidant, antinociceptive, anti-inflammatory, antifungal, and antihypertensive properties. This study aimed to evaluate the susceptibility of fungal strains to hecogenin acetate, assess its combination with a reference drug using the broth microdilution method, and analyze morphological changes from yeast to filamentous forms (hyphae and pseudohyphae) in Candida albicans, Candida tropicalis, and Candida krusei strains through microculture. The results revealed that hecogenin acetate exhibited fungistatic activity, although it showed a higher IC₅₀ compared to fluconazole. When combined with fluconazole, hecogenin acetate did not reduce the drug's efficacy against C. tropicalis, but it did reduce it against C. krusei. Hecogenin acetate displayed lower inhibition of growth inhibition than fluconazole and decreased its effectiveness in this aspect; however, it completely inhibited fungal yeast-hyphae switch at its highest concentration. These findings suggest that hecogenin acetate may provide complementary benefits, particularly due to its fungistatic effect and ability to inhibit fungal virulence.
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9. Genomic insights into Candidatus Argiopiplasma dusa, a bacterial symbiont of the wasp spider Argiope bruennichi.
9. 对Candidatus Argiopiplasma dusa的基因组学研究：一种与黄蜂蛛Argiope bruennichi共生的细菌PMID:日期:2026-01-07Bacterial symbionts were shown to have diverse but significant impacts on the fitness of their host in insects, but little is known on the symbionts of spiders and their interactions with their hosts. Here, we assembled and investigated the circular 575 kb genome of Candidatus Argiopiplasma dusa, a novel bacterial symbiont of the wasp spider Argiope bruennichi. Phylogenomic analysis placed this species within the phylum Mycoplasmatota, in a poorly characterized clade that may represent a new order-level lineage or affiliate with the Mycoplasmatales order. With 559 predicted genes, the genome is relatively small compared to other Mycoplasmatota genomes (on average 969 genes) and has a low GC content of ∼24%. While the genome encodes genes for proteins involved in glycolysis and fermentative acetate production, it revealed minimal biosynthetic capabilities with pathways for nucleotide, amino acid, and vitamin biosynthesis being absent in Ca. Argiopiplasma dusa. This suggests a metabolic dependence and an endosymbiotic lifestyle within the spider host. The symbiont was detected in A. bruennichi populations across the distribution range of the spider but appears to be absent in certain populations.
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10. Development and psychometric validation of a community microbiology literacy scale in the context of infectious diseases, antimicrobial resistance, and vaccination.
PMID:日期:2026-01-07Microbiology literacy has become increasingly important for public health and societal resilience, particularly in the context of emerging infectious diseases, antimicrobial resistance, and misinformation. However, standardized instruments for assessing Microbiology literacy at the community level remain limited. This study aimed to develop a valid and reliable tool to assess Microbiology literacy in the general population and to examine its multidimensional structure. This methodological, cross-sectional study developed the Community Microbiology Literacy Scale (CMLS). An initial item pool was generated based on international Microbiology books and World Health Organization (WHO) guidelines, followed by expert evaluation and content validity assessment using the Davis technique. Data were collected from 835 adults aged ≥18 years. Exploratory factor analysis (EFA) was conducted using data from 498 participants without imposing an a priori dimensional structure. A conceptually coherent four-factor structure emerged from the data, and the factors were named according to the content of their constituent items. This structure was subsequently tested by confirmatory factor analysis (CFA) in an independent subsample of 337 participants. The EFA identified a four-factor structure comprising Basic Microbiology Knowledge, Infection Control Awareness, Antibiotic and Antimicrobial Resistance Awareness, and Vaccine Awareness, explaining 60.66% of the total variance. CFA confirmed acceptable model fit. The scale demonstrated high internal consistency [Cronbach's alpha (α) = 0.925], with subscale Cronbach's α values ranging from 0.793 to 0.896. Higher Microbiology literacy scores were associated with older age, higher educational attainment, being a healthcare professional, prior Biology or Microbiology education, and awareness of the term "Microbiology" (P < .05). No significant differences were observed according to place of residence. The CMLS is a valid, reliable, and multidimensional instrument for assessing community-level Microbiology literacy and may support targeted educational and public health interventions.