MICROCHIMICA ACTA微化学学报
MICROCHIMICA ACTA(英文缩写 MICROCHIM ACTA),ISSN 0026-3672,eISSN 1436-5073,中文译名:微化学学报 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 6.408 | Q1 |
| 2022 | 5.700 | Q1 |
| 2023 | 5.300 | Q1 |
| 2024 | 5.300 | Q1 |
| 2025 | 5.400 | Q1 |
MICROCHIMICA ACTA 最新收录文献
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2. Development of a graphene oxide-based PEGylated fluorescent aptasensor with ultrahigh-sensitivity and anti-fouling properties for the detection of serum carcinoembryonic antigen levels.
PMID:日期:2026-09-21The specificity and sensitivity of aptamer-based serum biomarker assays can be compromised by nonspecific adsorption of proteins. In this study, we used the carcinoembryonic antigen (CEA) aptamer as a model to develop a fluorescent aptasensor capable of specifically and sensitively detecting serum biomarkers. This aptasensor employs the good antifouling capability of polyethylene glycol 2000 (P2K) to enhance specificity and the good fluorescence resonance energy transfer capability of graphene oxide (GO) to improve sensitivity. The aptasensor was applied to determine pure CEA protein and CEA in clinical serum and demonstrated that the P2K-modified fluorescent aptasensor exhibited significantly superior performance in CEA detection compared with the unmodified aptasensor, including accuracy, precision, linear range, antifouling properties, and selectivity. In the detection of CEA levels in clinical serum samples, the P2K-modified fluorescent aptasensor exhibited a lower limit of detection (LOD) (1.31 vs. 1.90 ng/mL), a wider linear range (1-256 vs. 1-64 ng/mL), better recovery (92.4%-103.6% vs. 73.3%-124.1%), and a smaller relative standard deviation (RSD) (0.7%-2.5% vs. 2.0%-6.4%). The aptasensor is capable of determining CEA levels within 90 min using only 1 µL of serum, with advantages of requiring no prefabrication of sensors, measuring fluorescence intensity in a conventional microplate reader, operating experimentally at room temperature, and high-throughput detection. Conclusively, this study successfully developed a GO-based P2K-modified fluorescent aptasensor capable of specifically, sensitively, and conveniently detecting CEA levels in clinical serum samples and with potential for clinical application.
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3. Photoactivated fluorescent nanozymes from mulberry leaf-derived carbon dots for dual-mode antioxidant determination and biothiol contribution estimation.
PMID:日期:2026-09-21Contribution-resolved antioxidant determination in complex food matrices remains challenging because the total antioxidant response usually arises from multiple overlapping reducing species. Herein, red-emissive carbon dots (CDs) were prepared from mulberry leaves via a solvothermal method and developed as highly efficient photoactivated fluorescent nanozymes for dual-mode antioxidant determination. The CDs exhibited a large Stokes shift (Δλ = 272 nm) and were stably dispersed in aqueous media with the assistance of Tween-20. Under 365 nm irradiation, the CDs showed oxidase-like activity toward 3,3',5,5'-tetramethylbenzidine (TMB), producing oxidized TMB (oxTMB) and simultaneously inducing fluorescence quenching through an inner-filter-effect-dominated process. In the presence of antioxidants, oxTMB was reduced, accompanied by color fading and fluorescence recovery, thereby establishing a light-gated "oxidation-quenching/reduction-recovery" dual-mode sensing platform. Using biothiols as model reductive analytes, the limits of detection for Cys and GSH were 0.29 and 0.16 µM in the colorimetric mode, and 0.34 and 0.26 µM in the fluorescent mode, respectively. More importantly, by integrating N-ethylmaleimide-mediated thiol blocking with total antioxidant measurement, the platform enabled differential estimation of the biothiol-associated reducing contribution in complex soy sauce samples. The total antioxidant capacity was determined to be 1392.3-2643.5 µM Cys-equivalent, while the biothiol-associated contribution was estimated to be 102.4-384.8 µM Cys-equivalent. This work provides a light-gated dual-mode nanozyme platform and a chemically selective strategy for contribution-resolved antioxidant determination in complex fermented food matrices.
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4. {"_":"DO metabolic labeling combined with AuNPs@PVP/SA hydrogel SERS platform for rapid quantitative detection of active lactic acid bacteria.","sub":["2"]}
PMID:日期:2026-09-21Achieving accurate quantification and counting of active lactic acid bacteria (LAB) is an important technical guarantee for the industrial production and application of LAB and their products. In this study, a gold nanoparticles@polyvinylpyrrolidone/sodium alginate (AuNPs@PVP/SA) hydrogel SERS sensor with outstanding stability and sensitivity was developed. Utilizing this platform as a substrate material to mitigate the "coffee ring" effect, we explored a strategy for the precise quantitative determination of mixed active LAB based on the AuNPs@PVP/SA hydrogel SERS platform and DO-probed Raman spectroscopy technology. The results indicated that the SERS platform based on AuNPs@PVP/SA hydrogel exhibited excellent spectral reproducibility for LAB as a SERS substrate material, with a significantly reduced "coffee ring" effect. This strategy enabled rapid and quantitative detection of active lactic acid bacteria (LAB), with a limit of detection (LOD) of 10 CFU/mL. Moreover, the strategy had excellent analytical capabilities for the qualitative identification and precise quantification of different species of active LAB in actual samples, achieving an average accuracy of 98.67% with RSD values all less than 5%. It provides a potential technical support for the dynamic monitoring of relative abundance and metabolic phenomena of mixed active LAB in co-culture systems during actual industrial production.
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5. {"_":"A novel carbon black-integrated cage-structured NiCoO nanocomposite for the selective and sensitive electrochemical determination of vanillin.","sub":["2","4"]}
PMID:日期:2026-09-18A novel electrochemical sensor for determination of vanillin based on carbon black and NiCoO nanocomposite (CB@NiCoO) was established. The nanocomposite was immobilized onto electrode surface, the carbon black can selectively adsorb vanillin via π-π stacking interactions between the aromatic domains and the benzene ring of vanillin. Concurrently, the NiCoO with cage-structure enhances the electrochemical signal response of the adsorbed vanillin by the high specific surface area and favorable ion-exchange capability. Under optimized experimental conditions, the sensor exhibits a linear range of 0.1 ~ 60 µM with a limit of detection of 30 nM, and the selectivity and signal stability were confirmed to be satisfactory. In real-sample analysis, the results are agreement with the contrast testing results of high-performance liquid chromatography, and the spike recovery experiment values ranged from 95.35% to 103.80%, with relative standard deviations (RSDs) of 3.77%~4.59% (n = 3). The proposed electrochemical sensor possesses a favorable application prospect in product quality control.
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6. An interface-regulated colorimetric-electrochemical dual-mode aptasensor for acetamiprid detection.
PMID:日期:2026-09-18Matrix-derived electroactive interference remains a major analytical challenge for electrochemical aptasensors in complex food samples. We develop a sequential dual-mode aptasensor based on interface regulation for reliable colorimetric screening and electrochemical quantification of acetamiprid. The aptasensor is constructed on a g‑C₃N₄/AuNP-modified electrode by immobilizing complementary DNA (cDNA), which hybridizes with AuPt@FeₓO nanozyme-labeled aptamer probes. Upon acetamiprid introduction, target-induced competitive binding drives partial dissociation of the nanozyme probes from the electrode surface. A subsequent PBS rinsing step enables controlled regulation of the sensing interface by removing dissociated probes and coexisting interferents. The released probes are collected for colorimetric screening based on nanozyme-catalyzed TMB oxidation over a concentration range from 10.0 pg mL⁻¹ to 1.0 µg mL⁻¹, followed by electrochemical quantification using the washed electrode over a range from 10.0 fg mL⁻¹ to 1.0 ng mL⁻¹. The limits of detection are 0.073 pg mL⁻¹ for the colorimetric mode and 0.495 fg mL⁻¹ for the electrochemical mode. In this sequential workflow, colorimetric screening offers rapid visual feedback while electrochemical quantification provides sensitive trace-level analysis; the two modes enable mutual signal validation, reducing false-positive and false-negative responses and improving analytical reliability. Satisfactory accuracy and reproducibility are achieved in real food samples, demonstrating that interface-mediated matrix decoupling is an effective strategy for robust aptasensing in complex matrices.
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7. Freeze-thaw-directed interfacial assembly of a gold nanoparticle/MXene electrochemical aptamer sensor for antibody-free myeloperoxidase detection.
PMID:日期:2026-09-18An electrochemical aptamer-based (E-AB) sensor for myeloperoxidase (MPO) was developed that combined a gold nanoparticle /MXene scaffold with freeze-thaw-directed aptamer immobilization and probe-density optimization. Unlike using nanocomposite amplification alone, this strategy regulates probe density and accessibility at the bioreceptor layer to improve target-coupled signal transduction. Using the same aptamer solution, the freeze-thaw method yielded 60.77% greater MPO-induced signal suppression and a 2.4-fold lower regression-derived limit of detection (LOD) than conventional incubation. After target incubation for 50 min at 4 °C, the sensor showed a linear response from 5.00 to 250.0 ng/mL in 1% plasma matrix (equivalent to 500 ~ 25,000 ng/mL in undiluted plasma), with an LOD of 4.15 ng/mL (equivalent to 415 ng/mL in undiluted plasma). These results illustrated that coupling conductive nanointerface design with aptamer-layer organization might provide a practical route for antibody-free and HO-independent MPO determination.
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8. A chiral silicon-based electrochemical sensor for the detection of L-glutamate.
PMID:日期:2026-09-18L-Glutamate (L-Glu), a major excitatory neurotransmitter in the central nervous system, plays an important role in nitrogen metabolism. In this study, chiral mesoporous imprinted silica nanoparticles (CMISNs) were synthesized via the sol-gel method using L-Glu derivatives as templates. Subsequently, the AuNPs/CMISNs@CMK-3/GCE electrochemical sensing interface was constructed. Benefiting from the chiral mesoporous structure of CMISNs and combined high conductivity of CMK-3 and signal amplification effect of gold nanoparticles (AuNPs), the designed electrochemical sensor enables specific detection of L-Glu. The developed sensor exhibits a wide linear response range for L-Glu (1 nM to 1 mM), with a limit of detection of 0.15 nM and a limit of quantification of 0.52 nM. It also demonstrated remarkable enantioselectivity, achieving a chiral separation factor of 7.7 toward glutamate enantiomers, along with good reproducibility (RSD = 3.2%, n = 5). The sensor retained excellent stability over 14 days and high selectivity against potential interferents. In contrast to conventional enzyme-based biosensors, chiral recognition is achieved by imprinted mesopores of L-Glu without the involvement of biological enzymes. This work provides a novel bionic sensing platform for the precise detection of L-Glu, which holds significant potential in neuroscience research and clinical diagnosis.
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9. Water-induced supramolecular self-assembly of porphyrin integrated with multivalent metal-organic framework for enhanced electrochemiluminescence and ultrasensitive aptasensing of 4-fluoromethamphetamine.
PMID:日期:2026-09-18Aggregation-caused quenching (ACQ) and poor aqueous solubility severely restrict electrochemiluminescence (ECL) applications of polycyclic aromatic hydrocarbons (PAHs). Herein, a water-induced supramolecular self-assembly strategy is proposed to address these drawbacks. Via a simple reprecipitation route, representative PAH meso-tetrakis(4-carboxyphenyl)porphyrin (TCPP) forms ordered supramolecular aggregates driven by noncovalent interactions, which suppress π-π stacking and ACQ and achieve a low triggering potential of - 1.0 V. Iron-based MOF supported molybdenum disulfide (MIL-101@MoS) is adopted as a multifunctional amplifier: reversible valence interconversion of Fe/Fe and Mo/Mo sustains continuous redox cycling to improve redox activity, while abundant oxygen vacancies accelerate electron transfer and coreactant activation for further ECL enhancement. After preparing MIL-101(Fe)@MoS@TCPP nanoparticles (MMST), an ECL aptasensor is fabricated for 4-fluoromethamphetamine (4-FMA) determination relying on target-triggered cyanine dye displacement. This sensor exhibits an ultralow limit of detection of (1.87 × 10 g/L) (S/N = 3) and a broad linear range from (1.0 × 10 - 1.0 × 10 g/L) g/L. It also shows favorable analytical results for real e-cigarette samples, with recoveries of 95.0-104.0% and relative standard deviations (RSD) less than 5.0%. This work offers a green route for high-performance PAH-based ECL luminophores and a reliable tool for rapid screening of emerging psychoactive contaminants in complicated food-related matrices.
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10. Efficient fluorescence strategy based on a DNA tetrahedral nanodevice for intracellular hypochlorous acid imaging.
PMID:日期:2026-09-17Hypochlorous acid (HClO) is a pivotal effector molecule in the immune defense system of living organisms and plays a critical role in maintaining physiological homeostasis. Aberrant HClO levels are reported to be associated with numerous diseases, making it a promising disease biomarker in clinical settings. In this study, an efficient fluorescence detection strategy based on a DNA tetrahedron was constructed, for specific HClO imaging in living cells. This method enables specific recognition of HClO through a phosphorothioate (PS) modification site. Under optimized conditions, this method showed a detection range of 0.05-5 µM, with a limit of detection of 31.5 nM, enabling highly sensitive and specific detection of HClO. Recovery tests futher confirm its stable and accurate detection performance in complex samples. Moreover, this method was successfully applied to HClO imaging in RAW 264.7 cells, demonstrating satisfactory results. Overall, the method proposed here has many advantages, including high sensitivity, excellent stability, strong resistance to interference, simple detection process, and fast response, offering it considerable potential for biochemical analysis and disease diagnosis.