JOURNAL OF APPLIED MICROBIOLOGY应用微生物学杂志
JOURNAL OF APPLIED MICROBIOLOGY(英文缩写 J APPL MICROBIOL),ISSN 1364-5072,eISSN 1365-2672,中文译名:应用微生物学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.059 | Q2 |
| 2022 | 4.000 | Q2 |
| 2023 | 3.200 | Q2 |
| 2024 | 3.200 | Q2 |
| 2025 | 2.800 | Q3 |
JOURNAL OF APPLIED MICROBIOLOGY 最新收录文献
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1. Intestinal mucus promotes microbial degradation of bamboo fibre in an in vitro giant panda gut fermentation system.
PMID:日期:2026-09-24In some hindgut-fermenting animals, the intestine secretes mucus that provides physical lubrication and protection under high-fiber dietary conditions, yet the role of this mucus in plant-fiber degradation by the gut microbiota remains unclear. The unique "mucus expulsion" of giant pandas offers an ideal model to investigate the physiological significance of mucus in herbivore dietary adaptation. We established an in-vitro gut-mimicking system with mucus as the key variable. We quantified bamboo fiber degradation, visualized microstructural damage via scanning electron microscopy (SEM), and analyzed the microbial community and functional potential using 16S rRNA sequencing. Null model-based ecological process inferences (NST, βNTI) were employed to probe community assembly mechanisms. In the mucus system, the degradation of bamboo cellulose and hemicellulose was significantly enhanced (P<0.05), with scanning electron microscope (SEM) revealing extensive damage to bamboo fiber tissues. This enhanced degradation was associated with the predicted microbial functions related to plant polysaccharide metabolism, including diverse hydrolases (e.g., GH2/3/43) and transport systems. The primary contributors to this genetic potential were enriched fiber-hydrolyzing genera, including Bacteroides and Dysgonomonas, which actively interacted with resident probiotics and other commensal bacteria. Community assembly analysis suggested that the mucus physicochemical environment imposed deterministic constraints on microbial community composition, while phylogenetic turnover remained within stochastic expectations and was primarily characterized by dispersal limitation. Within the mucus physicochemical environment, a specifically assembled microbial consortium was associated with enhanced bamboo fiber degradation and nutrient acquisition. These findings provide new insights into the potential role of mucus in supporting microbial adaptation to a bamboo-based diet in giant pandas.
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2. Antibacterial Potential and Polyphasic Taxonomy of Streptomyces Isolates From Extreme Habitats.
PMID:日期:2026-09-22Within the scope of combating global antibacterial resistance, this study aimed to evaluate the biotechnological and antibacterial potentials of Streptomyces isolates retrieved from two distinct extreme habitats in the Eastern Anatolia Region of Türkiye through polyphasic characterization and secondary metabolite production optimization. Soil physicochemical properties from extreme habitats were determined using potentiometric and conductometric methods, and Streptomyces isolates were retrieved via selective plating techniques. Following primary antibacterial screening using the cross-streak method, selected isolates underwent morphological, biochemical, and molecular characterization based on 16S rRNA, rpoB, and recA gene sequencing. Furthermore, the production of antibacterial metabolites by selected isolates was carried out by optimizing fermentation conditions to determine the most suitable culture media and cultivation periods. Among the isolates, 14.7% exhibited significant antibacterial activity. Polyphasic characterization identified three strategic isolates as Streptomyces rochei (BH011), Streptomyces bikiniensis (BH022), and Streptomyces inhibens (BH034), while BH006 and BH026 showed high phylogenetic similarity to Streptomyces ficellus and Streptomyces gobitricini, respectively. Fermentation optimization revealed GYM broth as the ideal medium for BH026, which demonstrated broad-spectrum activity, including a 25 mm inhibition zone against Escherichia coli. Conversely, isolate BH022 exhibited significantly enhanced antibacterial activity upon co-culture induction with Staphylococcus aureus. Our findings suggest that extreme environments in Türkiye represent significant hotspots for the identification potential biotechnological microbial sources. The enhancement of antibacterial activity through nutritional optimization and interspecies competition highlights the potential of these extreme-habitat isolates for further bioprospecting of bioactive secondary metabolites.
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3. Pathogenic Escherichia coli Isolated from Recreational Waters in Northwestern Wyoming Display Multidrug Resistance.
PMID:日期:2026-09-21To determine the prevalence, antimicrobial resistance, and genomic characteristics of antimicrobial-resistant Escherichia coli isolated from recreational freshwater in the Greys-Hoback watershed, Wyoming, USA. A total of 263 presumptive E. coli isolates were recovered using membrane filtration (EPA Method 1603), of which 218 (83%) were confirmed as E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial susceptibility testing by broth microdilution identified 20 (∼9%) isolates with clinically relevant resistance phenotypes, including 6 multidrug-resistant (MDR; ≥3 antimicrobial classes). Whole-genome sequencing of the resistant subset identified blaEC alleles in all genomes, along with additional resistance determinants, including sul2, tet, aph variants, and floR genes. Plasmid replicons were detected in most isolates, with IncF-type plasmids predominating and associated with MDR gene clusters. Contig-level analysis identified plasmid-associated MDR regions in selected isolates, although plasmid reconstruction was limited by short-read assemblies. Phylogenetic analyses revealed multiple sequence types and O: H serotypes, demonstrating substantial genetic diversity and suggesting that the resistant E. coli population originated from multiple sources rather than from clonal expansion. All sequenced isolates carried multiple virulence-associated genes, indicating co-occurrence of resistance and virulence determinants. By integrating antimicrobial susceptibility testing, whole-genome sequencing, and plasmid reconstruction, we show that recreational freshwater in northwestern Wyoming harbors genetically diverse antimicrobial-resistant pathogenic E. coli, including MDR isolates associated with putative mobile resistance elements. These findings support the inclusion of recreational water systems in environmental AMR surveillance frameworks and highlight the value of integrating phenotypic and genomic approaches for isolate characterization.
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4. In vitro digestion and fecal fermentation profiles of Cremastra appendiculata polysaccharide and evaluation of its antioxidant and hypoglycemic capacities.
PMID:日期:2026-09-16Cremastra appendiculata is a traditional Chinese herbal medicine for liver cancer treatment. C. appendiculata polysaccharides (CAP) as a biomacromolecular showed significant lipid-lowering activities. However, the gastrointestinal metabolic fate and the bioactivities of the fermented products remain poorly understood. Simulated salivary, gastric, intestinal digestion models, together with an in vitro human fecal fermentation system, were employed to evaluate the physicochemical profiles of CAP. Variations in molecular weight, reducing sugars, free monosaccharides, short-chain fatty acids (SCFAs), and pH were determined. 16S rRNA gene sequencing was performed to characterize alterations in fecal fermentation microbial communities, and the in vitro α-amylase and α-glucosidase inhibitory activities and antioxidant activities were assessed. As a result, during the simulated fermentation process, CAP remained structurally stable throughout simulated gastrointestinal digestion. During fecal fermentation, CAP was degraded by the fecal microbiota as the molecular weight decreased and the reducing sugars increased. Moreover, CAP significantly enhanced SCFAs production, particularly acetic acid and propionic acid, with a reduction in fermentation pH. CAP selectively enriched beneficial bacteria, including Bifidobacterium, Bacteroides, Lactobacillus and Odoribacter, while suppressing potentially harmful microorganisms, such as Proteobacteria, Bilophila, and Eggerthella. Furthermore, fecal fermentation of CAP enhanced inhibitory effects against α-amylase and α-glucosidase and antioxidant activities. These findings provide mechanistic insights into the health-promoting properties of CAP and support its future development as a functional food ingredient and gut microbiota regulator.
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5. Applied antibacterial and surface decontamination potential of methyl salicylate-rich Gaultheria fragrantissima essential oil.
PMID:日期:2026-09-16The persistence of biofilm-forming nosocomial pathogens on healthcare-associated surfaces represents a major challenge in infection control. This study aimed to evaluate the antibacterial, antibiofilm, membrane-disruptive, and surface decontamination potential of methyl salicylate-rich Gaultheria fragrantissima essential oil (GFEO) against clinically relevant pathogens. Antibacterial activity was determined by broth microdilution, while antibiofilm effects were assessed using crystal violet assays. Membrane-disruptive activity was evaluated by measuring cytoplasmic leakage, and synergistic interactions with antibiotics were analysed using checkerboard titration. Surface disinfection potential was examined using carrier-based assays on stainless steel, glass, and polystyrene surfaces. GFEO exhibited broad-spectrum antibacterial activity (MIC: 0.156-2.5 mg mL⁻¹) and significantly inhibited early-stage biofilm formation (69-92%). Rapid membrane permeabilization was observed within 10 min, with up to approx. 80% disruption at 2 × MIC after 60 min. Synergistic interactions were identified with selected antibiotics (FICI 0.25-0.5). In surface assays, GFEO achieved approximately 3 log₁₀ reductions for most strain-surface combinations within 20 min and up to 4.77 log₁₀ after 60 min. GFEO exhibits a potent membrane-mediated antimicrobial profile with strong antibiofilm and surface bactericidal activity, highlighting its potential as a natural agent for healthcare-associated infection control and environmental decontamination.
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6. Host-microbiome interactions and enteric pathogen infections - from early-life to long-term impacts on gut-brain communication.
PMID:日期:2026-09-15Interactions of the host with the microbes that constitute the intestinal microbiota are important for maintaining health and well-being within the gastrointestinal tract and beyond. Disruptions to these interactions, such as those that follow enteric pathogen infection or administration of antibiotics, can lead to persistent pathophysiology, particularly if disruptions occur during early development in neonatal life. Here we highlight the impacts of altered host-microbe interactions following insults across the life span and discuss emerging mechanisms of action promoting these disruptions (see Graphical Abstract). We also identify potential therapeutics to restore these important pathways and their mechanisms of action.
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7. Production of Entomopathogenic Fungi and Pathogenicity of Metarhizium anisopliae against Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae).
PMID:日期:2026-09-11To address the severe defoliation caused by the invasive bronze bug, Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae), this study investigated the mass production potential of three entomopathogenic fungal (EPF) strains and the specific biocontrol efficacy of Metarhizium anisopliae against this pest. Initially, Cordyceps javanica (LCBPF 11), M. anisopliae (LCBPF 31), and Beauveria bassiana (LCBPF 46) produced > 1 × 10⁹ conidia g⁻¹ in solid-state fermentation. To scale production, liquid culture was selected for C. javanica and M. anisopliae using industrial by-products (corn flour and molasses), while M. anisopliae was further compared in a rice flour-dextrose-yeast extract (M1) medium. Fitness assays revealed that while M. anisopliae blastospores (M1) were more heat-susceptible at 45°C (LT50 0.182 h) than aerial conidia (LT50 1.621 h), survival analysis of bioassays against T. peregrinus adults revealed highly significant differences among treatments. Remarkably, all M. anisopliae propagules achieved 100% mortality within five days, with blastospores produced in M1 medium reaching an LT50 of only 1.81 days. Furthermore, Cox proportional hazards modeling confirmed that these optimized blastospores (M1) presented a mortality risk 2.51 times higher than the commercial standard Metarril® (Hazard Ratio = 2.51; 95% CI: 1.90-3.31). To the best of our knowledge, this is the first report of M. anisopliae blastospores successfully infecting T. peregrinus. Taken altogether, these findings demonstrate the technical feasibility and significant potential of these rapid-acting infective propagules for the management of this forest pest.
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8. Perilla seed oil reshapes the rumen microbiome and increases fermentation end-products in vitro.
8. 紫苏籽油重塑瘤胃微生物组并增加体外发酵终产物PMID:日期:2026-09-09The effect of Perilla frutescens seed oil (PSO) on an in vitro rumen microbial ecosystem was evaluated by integrating fermentation measurements, microbiome profiling, metagenomics, and untargeted metabolomics. Rumen inoculum was incubated for 24 h with a control TMR substrate (CK), TMR supplemented with 23.7 mg of Perilla seeds per bottle (PS), or TMR supplemented with 8.5 μL of Perilla seed oil per bottle (PSO), with the PS and PSO treatments providing equivalent amounts of seed oil. Fermentation kinetics and volatile fatty acids were measured, and microbial and metabolic responses were characterized using 16S rRNA gene sequencing, metagenomics, KEGG and CAZy annotation, untargeted metabolomics and MetOrigin2 source tracing. PSO increased maximum gas production and total volatile fatty acid concentrations while maintaining pH within the physiological range. Community diversity was unchanged, but PSO altered microbial composition, including increases in Firmicutes, Verrucomicrobia, Vagococcus, Clostridium and Lactobacillus and decreases in Shigella sonnei and Methanosarcina sp. Ant1. PSO also altered microbial functional profiles and increased several lipid- and vitamin-associated metabolites, including linoleic acid, 13-HODE, 9-oxoODE, pantothenic acid and thiamine, while reducing lactate. PSO changed rumen microbial community structure and functional potential in parallel with increased fermentation end-products and extensive metabolic shifts. These in vitro findings identify microbial and metabolic responses that warrant validation in vivo.
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9. Global Diffusion of IncC Plasmid Harboring blaNDM-1in the High-Risk Escherichia coli ST131 Clone.
PMID:日期:2026-09-09The global expansion of quinolone-resistant Escherichia coli (QR-EC) is increasingly associated with β-lactam resistance and mobile genetic elements that facilitate resistance dissemination. This study investigated the molecular mechanisms underlying fluoroquinolone and β-lactam resistance in clinical QR-EC isolates and explored the plasmid type associated. A total of 123 non-duplicate QR-EC clinical isolates responsible mainly for gastrointestinal colonization were collected between 2019 and 2021. Plasmid-mediated quinolone resistance (PMQR), extended-spectrum β-lactamase (ESBL), and carbapenemase genes were screened by PCR. Mutations in the quinolone resistance-determining regions (QRDR) of gyrA and parC were analyzed using sequencing and mismatch amplification mutation assay PCR (MAMA-PCR). Selected isolates underwent multilocus sequence typing (MLST). Whole-genome sequencing (WGS) of a representative extensively drug-resistant strain carrying multiple quinolone resistance determinants, ESBL genes, and carbapenemase genes, was performed. PMQR genes were prevalent among QR-EC, dominated by aac(6')-Ib-cr (60.9% of isolates). ESBL genes were identified in 93.5% of isolates, predominantly blaCTX-M (95.7%). Among ertapenem-resistant isolates (QCR-EC) (n=18), blaNDM-1 and blaOXA-48 were detected in 13 and 11 isolates, respectively. QRDR mutations were highly frequent, particularly gyrA83 (98.4%) and parC80 (30.9%). Major QCR-EC genotypes belonged to sequence types ST167 (n=2), ST1196, ST469, and ST410. High-risk E. coli ST131 clone harboring IncC plasmid encoding blaNDM-1 was described for the first time in Africa, following its emergence, in two continents, Asia and America. Despite the very rare description of these strains worldwide, their description in three continents sign their global diffusion. This finding highlights the ongoing spread of carbapenem resistance and underscores the urgent need for strengthened genomic surveillance.
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10. Rethinking escape frequency benchmarks for environmental applications of engineered microorganisms.
PMID:日期:2026-09-03Biological containment strategies are widely used to reduce possible risks associated with genetically modified microorganisms (GMMs). In the biosafety literature, the performance of these systems is often benchmarked against a maximum escape frequency of one cell per 108 cells. This value is commonly attributed to the USA National Institutes of Health Guidelines for Research Involving Recombinant DNA. However, the guideline refers specifically to laboratory certification of certain host-vector systems and does not define an acceptable escape frequency for applications outside controlled laboratory environments. Despite this limited scope, the 10-8 criterion has been repeatedly cited in research articles and reviews as a general biosafety standard for GMMs. At the same time, quantitative data on escape frequency and survival of genetically modified microorganisms under realistic environmental conditions remain scarce. This lack of empirical evidence complicates environmental risk assessment and can hinder regulatory approval and technology transfer for applications intended to operate beyond the laboratory. Here, we clarify the origin and scope of the 10-8 escape criterion and discuss why it should not be interpreted as a universal biosafety standard. We argue that experimentally validated measurements of escape and survival under application-relevant conditions are urgently needed to support evidence-based biosafety assessment and the responsible development and deployment of GMMs.