STEM CELLS干细胞
STEM CELLS(英文缩写 STEM CELLS),ISSN 1066-5099,eISSN 1549-4918,中文译名:干细胞 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.845 | Q1 |
| 2022 | 5.200 | Q1 |
| 2023 | 4.000 | Q1 |
| 2024 | 3.600 | Q2 |
| 2025 | 4.800 | Q1 |
STEM CELLS 最新收录文献
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1. Characterization of Carcinogenesis Resistance in Anatolian Blind Mole-rat Mesenchymal Stem Cells and Assessment of Transcriptomic Signatures.
PMID:日期:2026-09-25Mesenchymal stem cells (MSCs) exhibit dual roles in tumor progression and therapy resistance. However, the blind mole-rat (BMR, Nannospalax xanthodon) exhibits extraordinary natural resistance to spontaneous and induced carcinogenesis, making its MSCs a subject of significant therapeutic interest. This study investigates BMR MSCs' and fibroblasts' resistance to the carcinogen N-methyl-N-nitrosourea (MNU). We examined the cellular behavior and transcriptomic expression differences of bone marrow-derived MSCs and also fibroblasts isolated and characterized from blind mole-rats after exposure to the chemical carcinogen MNU. Similar analyses were also performed on mouse, rat, and human-derived MSCs, and expression disparities between species were evaluated. While MNU treatment significantly induced apoptosis and reduced cell viability in human and mouse MSCs, BMR MSCs maintained stable proliferation kinetics, viability, and multi-lineage differentiation potential. Transcriptomic profiling revealed species-specific gene expression patterns, highlighting unique adaptive responses in the BMR. In BMR fibroblasts, MNU treatment suppressed PI3K/Akt signaling and modulated mitochondrial oxidative phosphorylation, whereas BMR MSCs demonstrated a flexible signaling architecture that preserved cellular programming and p53-mediated transcriptional regulation. Cross-species analysis integrated with TCGA datasets identified LAMTOR3 as a critical p53-dependent tumor-protective node. The ability of BMR MSCs to maintain the p53-LAMTOR3 axis following MNU treatment, alongside the suppression of pro-tumoral SMAD signaling, suggests a robust evolutionary strategy to inhibit malignant transformation. These findings provide novel insights into the genomic integrity of the BMR and identify potential biomarkers and therapeutic targets for enhancing cancer resistance.
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2. EPLIN stabilizes cell-cell adhesion complexes to facilitate TGF-β1-induced fibroblastic differentiation of human periodontal ligament stem cells.
PMID:日期:2026-09-24Periodontal ligament stem cells (hPDLSCs) are essential for periodontal regeneration, yet the molecular mechanisms governing their differentiation into functional fibroblasts remain poorly understood. This study aimed to identify novel regulatory molecules of PDL fibroblastic differentiation and elucidate the role of Epithelial Protein Lost In Neoplasm (EPLIN) in the fibroblastic differentiation. hPDLSCs were differentiated into PDL fibroblasts using TGF-β1. A novel antibody (anti-LG45) identified EPLIN as a target antigen. Functional roles of EPLIN were investigated through siRNA-mediated depletion and ectopic overexpression. Phalloidin staining for F-actin, and co-immunoprecipitation were performed to analyze adherens junction integrity. TGF-β1 treatment successfully induced fibroblastic differentiation, as evidenced by significant increases in fibroblastic marker expressions, which were abolished by the TGF-β receptor inhibitor SB-431542. A novel Anti-LG45 antibody was found to specifically recognize EPLIN in differentiated PDL fibroblasts. EPLIN depletion significantly reduced the fibroblastic differentiation and disrupted the typical elongated morphology of PDL fibroblasts. Mechanistically, EPLIN depletion led to filament fragmentation and cytoskeletal collapse, decoupled N-cadherin from the cytoskeleton, and accelerated cell proliferation. Conversely, ectopic overexpression of EPLIN-α or β was sufficient to inhibit proliferation and induce fibroblastic markers even without TGF-β1 stimulation. EPLIN is a key regulatory factor that stabilizes the linkage between the actin cytoskeleton and adherens junctions, maintaining the fibroblastic phenotype and preventing unscheduled proliferation in hPDLSCs. This study indicates the importance of cytoskeletal structural integrity in PDL differentiation. Under this circumstance, EPLIN serves as a regulator that maintains the fibroblastic differentiation state of hPDLSCs and prevents unscheduled proliferation.
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3. Exploring Australian Public Attitudes Toward Organoid Research and Use: A Cross-Sectional Study.
PMID:日期:2026-09-24Organoid technologies offer promising new ways to study human biology and develop innovative therapies. While their scientific potential is clear, public awareness and acceptability remain largely unexplored - critical factors for ethical and equitable implementation. We conducted the first national survey of Australians (N = 502) to assess awareness, knowledge, attitudes, and acceptability of organoid technology. Awareness of stem cells was high, but organoids were largely unfamiliar. After a brief explanatory video, participants appeared to have more accurate conceptual framing (e.g., organoids as "research tools" and "mini-organs"), though objective knowledge remained limited. There was strong support for organoids, and more people expressed willingness to donate cells for stem cell or organoid research than have previously done so. Acceptability was correlated with attitudes toward animal research, knowledge, and some sociodemographic factors. Overall, public endorsement of organoid technology appears high but conditional, underscoring the need for transparent governance, clear consent, and inclusive communication.
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4. Expression of Concern: Hyaluronan Is Crucial for Stem Cell Differentiation into Smooth Muscle Lineage.
PMID:日期:2026-09-22该文献暂无摘要。
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5. Human umbilical cord mesenchymal stem cells enhance folliculogenesis and fertility in premature ovarian failure mice via regulating steroid synthesis.
PMID:日期:2026-09-22Premature ovarian failure (POF) is a dysfunction that severely impacts women's physiological health and reproductive function. Human umbilical cord mesenchymal stem cell (hUCMSC) transplantation offers a promising therapeutic option for restoring ovarian function. However, its efficacy and underlying mechanisms are not yet fully understood. A POF mouse model was established using cyclophosphamide induction, and hUCMSCs were transplanted into the ovaries through in situ injection. Treatment efficacy was evaluated by assessing hormone levels, estrous cycle, follicle count, and reproductive outcomes. Furthermore, metabolomic sequencing was performed on the ovarian tissues of mice to explore the mechanism underlying the therapeutic effect of hUCMSCs. Compared with the POF control group, the hUCMSC treatment group showed significant improvements: primordial and primary follicle counts increased by 8-fold and 5-fold, respectively, while atretic follicle ratios decreased by 50%. Pregnancy rates in treatment group increased from 30% to 60%, with average litter sizes rising from 1.6 pups/litter to 4.1 pups/litter. The expression of key genes (Cyp11a1, Cyp19a1, Star) in the steroidogenesis pathway was significantly upregulated in ovarian tissues, which restored the levels of steroid hormones such as progesterone and thereby stabilized serum sex hormone levels. In situ injection of hUCMSCs can restore ovarian function and fertility in POF mice by reducing granulosa cell apoptosis and upregulating steroidogenesis pathways.
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6. Transverse tibial transport accelerated of diabetic wound healing via enhanced Wnt/β-catenin-driven BMSCs mobilization and angiogenesis.
PMID:日期:2026-09-22The management of severe diabetic foot ulcers (DFUs) remains a major clinical challenge. Tibial cortex transverse transport (TTT) has emerged as an effective surgical intervention, but its underlying mechanism, particularly concerning stem cell mobilization and signaling pathways, is largely unknown. A diabetic DFU model was established in New Zealand white rabbits using Streptozotocin (STZ), followed by TTT surgery. Wound healing was evaluated through photography, H&E, and Masson's trichrome staining. Angiogenesis was assessed by high-resolution X-ray angiography and immunofluorescence. Bone marrow-derived mesenchymal stem cell (BMSC) mobilization was analyzed via CFU-F assays and flow cytometry. Serum levels of SDF-1α, CXCR4, VEGF, and Ang2 were measured by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction. The activity of the Wnt/β-catenin pathway was determined by Western blot. Tibial cortex transverse transport significantly accelerated wound closure and enhanced tissue regeneration, with improved epidermal/dermal restoration and collagen deposition. Consistently, neovascularization in wounds was markedly promoted. Mechanistically, TTT activated the Wnt/β‑catenin signaling pathway in BMSCs, which was associated with enhanced BMSC quantity, mobilization, and migration capacity. Inhibition of this pathway attenuated the TTT‑induced pro‑healing effects. Tibial cortex transverse transport promotes healing of DFUs primarily by activating the Wnt/β-catenin pathway in BMSCs, thereby enhancing their mobilization, homing, and pro-angiogenic capacity. This study reveals a key molecular mechanism underlying TTT's efficacy and supports its therapeutic potential for refractory Diabetic foot ulcer (DFU).
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7. Mesenchymal Stem Cell Therapy for Ischemic Stroke: Bridging Clinical Evidence and Translational Challenges.
PMID:日期:2026-09-21Ischemic stroke remains a major cause of long-term disability worldwide. Despite advances in acute reperfusion therapies, many survivors experience persistent neurological deficits due to irreversible neuronal loss and limited endogenous repair, highlighting the need for effective regenerative strategies. Mesenchymal stem cells (MSCs) have emerged as promising candidates for cell-based therapy because of their multimodal paracrine effects, including immunomodulation, promotion of angiogenesis and neurogenesis, attenuation of neuroinflammation, and enhancement of endogenous neuroplasticity. This Concise Review synthesizes the current clinical evidence supporting MSC therapy for ischemic stroke, critically examines the biological and translational determinants of therapeutic efficacy, and discusses emerging strategies, including engineered MSCs, extracellular vesicle (EV)-based therapies, and integration with neurorehabilitation. A structured literature search of PubMed/MEDLINE, Embase, Web of Science, and the Cochrane Library through June 2026 prioritized randomized controlled trials, systematic reviews, and meta-analyses. The largest contemporary meta-analysis, encompassing 30 clinical studies and 1,217 participants, confirmed the favorable safety profile of MSC therapy while emphasizing the need for larger, standardized randomized trials to establish definitive clinical efficacy. Available evidence indicates that MSC therapy is generally safe, with serious treatment-related adverse events being uncommon, and may improve neurological impairment and functional recovery. However, heterogeneity in cell source, manufacturing protocols, administration route, treatment timing, dosing strategies, and patient selection continues to limit definitive conclusions regarding efficacy. Future progress will depend on standardized Good Manufacturing Practice-compliant manufacturing, validated potency assays, optimized patient selection, identification of the optimal therapeutic window, and integration of engineered MSCs, EV-based therapies, and combination approaches with neuromodulation.
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8. Immune cell development from human pluripotent stem cells.
PMID:日期:2026-08-26The landmark derivation of induced pluripotent stem cells (iPSCs) sparked an enormous range of research on development of diverse cell populations. Perhaps no aspect of this work has been as productive as studies on blood and immune cell production. Indeed, essentially all human blood cell populations can be derived from human iPSCs. While derivation of transplantable hematopoietic stem cells (HSCs) from iPSCs has been relatively challenging, clinical translation of iPSC-derived immune cells has been particularly productive. Notably, more patients have been treated with iPSC-derived natural killer (NK) cells than any other iPSC-derived cell type. Use of iPSCs provides a key platform to incorporate multiplexed gene edits before differentiation, enabling NK cells to be engineered with tumor-targeting CARs, cytokine support for improved persistence, enhanced tumor trafficking, and resistance to host immune rejection, modifications uniformly expressed across the entire cellular product. While T cells, macrophages and other immune cells can be produced from human iPSCs, iPSC-derived NK cells have been the most widely used in clinical trials for treatment of refractory malignancies, as well as autoimmune disease. This review summarizes research with a focus on clinical translation of iPSC-derived immune cells, as well as highlights continued challenges and prospects of this field.
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9. Donor sex modulates hiPSC-derived cardiomyocyte metabolic and functional response to fatty acids.
PMID:日期:2026-08-22Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) enable cardiovascular disease modeling and regenerative research but exhibit substantial heterogeneity and incomplete metabolic maturation. Palmitate (PA) and oleate (OA) can promote aerobic maturation, although responses vary. Despite sex differences in adult cardiac metabolism, sex effects in hiPSC-CMs remain poorly defined. We assessed sex-dependent metabolic and functional responses in hiPSC-CMs from three male and three female lines treated with PA or OA. Contractile function, aerobic metabolism, and transcriptomic profiles were evaluated. Sex differences were modest at baseline but pronounced after FA treatment. Male- and female-derived hiPSC-CMs responded differently to PA and OA across contractile, metabolic, and transcriptional measures, indicating that FA identity and sex jointly shape hiPSC-CM phenotypes. Sex contributes to hiPSC-CM heterogeneity and modulates responses to FA-based maturation. Incorporating sex-specific metabolic and functional regulation may improve the consistency and physiological relevance of hiPSC-CM maturation strategies.
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10. Loss of ABCA4 from photoreceptor discs is associated with glial transcriptomic changes in retinal organoids.
10. 感光盘ABCA4的缺失与视网膜类器官中的神经胶质转录组变化有关PMID:日期:2026-08-22Loss-of-function mutations in the ABCA4 gene cause Stargardt disease (STGD1), the most common inherited macular dystrophy leading to progressive central vision loss. Here, we generated human induced pluripotent stem cell-derived retinal organoids harboring a premature stop codon in exon-24 of ABCA4 to evaluate the impact of this mutation on mRNA and protein levels in a human model. Immunofluorescence analysis revealed the absence of ABCA4 protein in the mutant photoreceptor outer segment discs, while single-cell RNA sequencing detected no major transcriptional alterations in rods and cones. Unexpectedly, differential gene expression and pathway enrichment analyses of Müller glial cells and astrocytes highlighted disruption of neuronal development, microenvironment of glial cells, intercellular communication, and programmed cell death pathways. These findings suggest that ABCA4 deficiency in photoreceptor discs may trigger early stress-associated transcriptomic responses in retinal glial cells prior to overt photoreceptor degeneration, potentially contributing to Stargardt disease pathogenesis.