MICROBIOLOGICAL RESEARCH微生物学研究
MICROBIOLOGICAL RESEARCH(英文缩写 MICROBIOL RES),ISSN 0944-5013,eISSN 1618-0623,中文译名:微生物学研究 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.070 | Q2 |
| 2022 | 6.700 | Q1 |
| 2023 | 6.100 | Q1 |
| 2024 | 6.900 | Q1 |
| 2025 | 8.500 | Q1 |
MICROBIOLOGICAL RESEARCH 最新收录文献
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1. An N-acetyltransferase influences AreA nuclear localization and nitrogen utilization in the basidiomycete Ganoderma lucidum.
PMID:日期:2026-09-29Nitrogen metabolism is fundamental to fungal growth, development, and the biosynthesis of bioactive metabolites. The GATA transcription factor AreA plays a pivotal regulatory role in nitrogen metabolite repression. While the molecular mechanisms by which AreA responds to nitrogen sources have been relatively well elucidated in ascomycetes, they remain poorly understood in basidiomycetes. Here, using the macrofungus Ganoderma lucidum as a model, we elucidated a molecular mechanism by which an N-acetyltransferase (Nat) influences AreA nuclear entry under non-preferred nitrogen sources, thereby promoting nitrogen catabolic gene expression and the utilization of non-preferred nitrogen sources. Through a yeast two-hybrid library screen, we identified Nat as an AreA-interacting protein, and their physical interaction was subsequently validated by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. In vitro acetylation assays coupled with mass spectrometry analysis demonstrated that Nat specifically acetylates lysine residues 619 and 916 of AreA. In vivo experiments further showed that silencing of nat reduced the acetylation level of AreA. Nitrate conditions induced the expression of nat and increased NAT protein levels, compared with ammonium conditions. Silencing of nat did not affect areA transcription or total AreA protein abundance; however, it significantly reduced nuclear AreA accumulation with a concomitant increase in cytoplasmic retention. Compared with the wild-type strain, nuclear AreA accumulation in the nat-silenced strain was reduced by approximately 70% under nitrate conditions. Consequently, the expression and enzymatic activity of AreA-regulated nitrogen metabolic genes, including glutamine synthetase (gs) and nitrate reductase (nr), were markedly suppressed. This study illuminates a post-translational mechanism by which non-preferred nitrogen sources activate AreA in basidiomycetes. It further reveals a role for protein acetylation in the nitrogen metabolism of G. lucidum, thereby expanding our understanding of nitrogen metabolic networks in fungi.
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2. From oxygen detoxification to peroxide supply: Microbial NADH oxidases in redox physiology and H₂O₂-coupled biocatalysis.
2. 从氧气解毒到过氧化物供应：微生物NADH氧化酶在氧化还原生理学与H₂O₂偶联生物催化中的作用PMID:日期:2026-09-23NADH oxidases (NOXs) are flavin adenine dinucleotide (FAD)-dependent flavoproteins widely distributed in bacteria, archaea, and other microorganisms, where they couple NADH oxidation to oxygen reduction and contribute to redox homeostasis, oxygen adaptation, and peroxide metabolism. H₂O-forming NOX has traditionally been preferred for cofactor regeneration, whereas H₂O₂-forming NOX is increasingly recognized as a dual-function catalyst for NAD⁺ regeneration and in situ H₂O₂ production. This review summarizes the catalytic mechanisms, structural basis, and physiological functions of NOX, focusing on the biological and engineering potential of H₂O₂-forming NOX for in situ H₂O₂ generation and controlled utilization. It also discusses strategies for enhancing stability through protein engineering and process integration, particularly in peroxide-dependent cascade systems, using the H₂O₂-forming NOX/unspecific peroxygenase (UPO) cascade as a representative model. Finally, it highlights how native microbial redox functions can inspire NOX-centered H₂O₂-coupled biocatalytic systems that integrate cofactor regeneration, oxidant supply, and peroxide-flux control.
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3. Microbial communities and volatile profiles heterogeneity in a Mars-analogue volcanic lake.
3. 火星模拟火山湖泊中微生物群落与挥发性成分的异质性PMID:日期:2026-09-20Analyses of the Martian surface suggest the existence of sedimentary lacustrine deposits that may have once harboured life. Lake Bagno dell'Acqua (Pantelleria, Italy) represents an optimal planetary field analogue for investigating the evolution and adaptation of life in Mars-like environments and for validating potential life-detection strategies. Accordingly, we characterised microbial communities and volatile organic compound profiles in actively growing organosedimentary structures within lake sediments and in biofilm covering hot springs at the lake margins. The microbial communities exhibited low taxonomic diversity and were dominated by a few extremophilic and extremotolerant taxa. Only a marginal difference in community composition was detected between sediment and biofilm samples; however, high variability was reported among the sampling sites and the replicates of each site. An exploratory volatile compound profiling revealed the presence of numerous terpenes, with small variability among sampling sites. The specialized assemblages detected in the Bagno dell'Acqua environment indicate that similar consortia could have adapted to analogous settings on ancient Mars. Patterns identified in these communities may serve as biosignatures, including redox imbalances generated by organisms driving oxidation-reduction processes. Moreover, pronounced fine-scale heterogeneity provides valuable insights into life-detection strategies in comparable extraterrestrial environments. The abundance of terpenes, ancient, widely distributed biomolecules across all domains of life, indicates that similar molecular adaptations may likewise have arisen in similar environments elsewhere. If preserved, these volatiles could represent promising biosignatures in clay-rich lacustrine sediments, such as those that were widespread on Noachian Mars and are abundant in the sediments of Lake Bagno dell'Acqua.
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4. Genome-resolved metagenomics reveals lineage-specific phylogenomic and functional differentiation among carbon-processing bacteria from mangrove and terrestrial forests.
PMID:日期:2026-09-16Microbial carbon processing in mangrove and terrestrial forests remains poorly resolved at the level of individual bacterial genomes. Here, shotgun metagenomics was used to compare bacterial communities and genome-encoded carbon-processing potential across mangrove sediments, terrestrial forest soils, and associated litter from Goa, India. Genome reconstruction recovered 15 bacterial metagenome-assembled genomes (MAGs), including eight phylogenomically distinct, species-unresolved lineages identified through genome-based taxonomic and relatedness analyses. Community profiles broadly overlapped between habitats, with the strongest contrasts emerging among reconstructed lineages and their functional repertoires. The recovered genomes differed in carbohydrate-processing and bioelement-associated functions, revealing lineage-specific organization of carbon-processing potential. The mangrove-associated Paracoccus marcusii encoded RuBisCO-associated genes together with C1- and sulfur-associated functions, representing a distinctive carbon- and redox-associated genomic repertoire. Validated carbonic anhydrase classes also varied among bacterial lineages. Carbohydrate-active enzyme profiles revealed pronounced substrate-level differentiation, with the mangrove-derived Verrucomicrobiota-affiliated JAAUTS01 lineage showing the broadest divergence from its close reference across multiple plant- and detritus-associated carbohydrate functions. Terrestrial-derived Pseudomonas_E and Sphingobacteriaceae-affiliated lineages displayed distinct restructuring across substrate-, bioelement-, and carbonic-anhydrase-associated traits, whereas several other MAG-reference pairs remained comparatively conserved, showing that genomic divergence does not uniformly translate into broad functional novelty. Exploratory carbon-strategy indices showed directionally higher stabilization scores among mangrove-derived MAGs and provided a transparent framework for testing genome-derived carbon traits against independently replicated environmental and process measurements. These findings show that carbon-processing potential is organized primarily at the lineage and functional-repertoire level and identify candidate genomic traits for future microbiome-informed carbon monitoring across blue-carbon and terrestrial forest systems.
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5. Multi-omics insights reveal a divergent type III secretion system-associated secretome in plant growth-promoting rhizobacterial Pseudomonas fluorescens.
PMID:日期:2026-09-16The type III secretion system (T3SS) is a well-characterized virulence apparatus in pathogenic bacteria; however, its role in beneficial rhizobacteria remains poorly defined. In the current study, we integrated multi-omics analyses with molecular genetics to systematically characterize the regulatory network and secretory output of the T3SS in the plant growth-promoting rhizobacterium (PGPR) Pseudomonas fluorescens 2P24. The canonical effector repertoire of 2P24 is markedly streamlined, and the conserved effector homolog HopM1 has lost virulence-associated activities. The sigma factor RspL is a root-responsive regulator that reprograms 31.92% of detected bacterial genes, promoting energy generation and metabolic pathways while repressing behavioral and signaling programs. Furthermore, T3SS integrity directly governs extracellular protein allocation by facilitating the secretion of non-canonical metabolic enzymes-including a validated hydrolase and decarboxylase-while restricting the release of flagellar components. Collectively, these findings establish the RspL-T3SS axis as an integrated regulatory and secretory module that coordinates transcriptional reprogramming with selective protein secretion during root interaction. This study expands our understanding of the classic virulence-associated T3SS as an adaptive machinery for rhizosphere colonization and yields mechanistic insights into bacterial rhizosphere fitness.
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6. Comparative genomic and phenotypic biosafety assessment of plant-associated Klebsiella pneumoniae species complex strains isolated from faba bean root nodules.
PMID:日期:2026-09-16Plant-associated members of the Klebsiella pneumoniae species complex (KpSC) may harbor genomic traits of potential agricultural relevance while also carrying resistance- and virulence-associated determinants of biosafety concern. Here, whole-genome sequencing and comparative genomic analyses were used to characterize nine KpSC strains isolated from Vicia faba root nodules, comprising three Klebsiella quasipneumoniae and six Klebsiella variicola isolates. Genome-based taxonomic analysis identified four sequence types (ST-355, ST-3956, ST-4200, and ST-4574), with phylogenomic analysis indicating that genomic relatedness was more strongly associated with sequence type than isolation source. Comparative analyses revealed lineage-associated differences in resistance- and virulence-associated genomic profiles, while multidrug non-susceptibility was phenotypically observed in K. quasipneumoniae NGB-FR79 and K. variicola NGB-FR73 and NGB-FR75. The genomes also carried genes putatively associated with agriculturally relevant traits, including nitrogen fixation, phosphorus metabolism, iron acquisition, auxin-associated pathways, and adaptation to abiotic stresses. Phenotypic assays further evaluated tolerance to salt and elevated temperature. Collectively, these findings demonstrate that nodule-associated KpSC strains combine putative plant-beneficial genomic traits with resistance- and virulence-associated determinants, emphasizing the need for strain-level genomic and phenotypic biosafety assessment before consideration for agricultural applications.
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7. A mycovirus from entomogenous fungi Beauveria acts as a mobile effector of phytopathogen antagonism through cross-taxonomic transmission.
PMID:日期:2026-09-16Fungal antagonism shapes microbial community structure and provides an ecological basis for biological control. Although mycoviruses have been implicated in fungal antagonism, their effects are generally attributed to indirect modulation of resident host physiology. Here, we show that a mycovirus can function as a mobile effector of antagonism through cross-taxonomic transmission, representing the first documentation of viral transfer from an entomopathogenic/endophytic fungus (Beauveria) to a phytopathogenic fungus (Sclerotinia) via a plant‑mediated route. We characterized Beauveria brongniartii victorivirus 1 (BbrVV1), a totivirus infecting the plant-colonizing entomopathogenic fungus Beauveria brongniartii. BbrVV1 enhanced the in planta suppression of Sclerotinia sclerotiorum by B. brongniartii, achieving a biocontrol efficacy of up to 66.7% at 3 days post‑inoculation, compared to 49.1% for the virus‑free strain, an effect associated with transmission of BbrVV1 from B. brongniartii to the invading pathogen within colonized plants. In its native host, BbrVV1 caused only moderate fitness effects, whereas host shift to S. sclerotiorum induced pronounced hypovirulence, including restricted vegetative growth, impaired sclerotial development and attenuated pathogenicity. Furthermore, based on in vitro dual‑culture and in planta co‑inoculation assays, BbrVV1 was found to spread horizontally within S. sclerotiorum populations via hyphal contact, but was not transmitted through mono-sclerotial progeny. These findings expand the ecological significance of mycoviruses as mobile agents of fungal interaction and provide a conceptual foundation for plant-associated viral delivery in biological control.
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8. Efficient scarless gene editing in Pichia pastoris via survival stress-based intramolecular homologous recombination.
PMID:日期:2026-09-16To overcome low efficiency and/or genomic instability induced by DNA cleavage in current genome-editing approaches, a novel pop-in/pop-out-based editing system was developed for Pichia pastoris. An ingenious arrangement of components leads to a more efficient screening by permitting the only type of DNA recombination under defined pressure conditions, in terms of the overall efficiency of gene editing, the system virtually depends on the integration efficiency mediated by single-crossover recombination. It does not rely on exogenous recombinases or programmable nucleases such as Cas9, thereby avoiding nuclease induced double strand breaks and associated off target mutations or chromatin fatigue. This strategy preserves high editing efficiency with no modification to the host's inherent genetic properties. Relative to site-specific recombination methods, its dual MazF counterselection enables seamless editing, avoiding scar sequence-induced genomic instability. In this study, nearly 100% knockout efficiency and over 86.67% integration efficiency were achieved in the described experimental cases with this system, which provides a new gene-editing tool for synthetic biology in Pichia pastoris.
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9. Ethanol-driven biosynthesis of polyhydroxyalkanoates by a newly isolated wild-type Cupriavidus strain.
PMID:日期:2026-09-16Ethanol has emerged as an attractive and sustainable feedstock for biomanufacturing because of its high atom economy and direct conversion to acetyl-CoA. However, its use for polyhydroxyalkanoate (PHA) production remains limited by the scarcity of microbial chassis. Here, we report a newly isolated wild-type strain, Cupriavidus sp. ZWJ01, obtained through ethanol-enrichment screening of soil samples collected from Pu'er City, Yunnan, China. This strain exhibits an exceptional innate capacity for ethanol-based PHA biosynthesis. Fermentation profiling under diverse carbon regimes revealed robust ethanol tolerance, sustained growth, and efficient polymer accumulation. After culture optimization, this unengineered strain produced 5.07 g/L poly(3-hydroxybutyrate) (PHB) with a conversion yield of 0.51 g/g ethanol, corresponding to 55% of the theoretical maximum. In addition, co-feeding ethanol with secondary substrates enabled the flexible synthesis of the functional copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB) with tunable monomer composition. Downstream characterization, including molecular weight analysis, further confirmed the structural integrity and material potential. Collectively, this work highlights ethanol as a highly efficient feedstock and establishes a robust native platform for the sustainable production of diverse high-value PHAs.
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10. CRISPR-Cas9 engineering for high-level β-galactosidase production in Saccharomyces boulardii.
PMID:日期:2026-09-15Saccharomyces boulardii is a clinically validated probiotic yeast widely used to treat gastrointestinal disorders. Its natural probiotic effects, combined with the capacity of surviving the harsh gastrointestinal conditions, makes it an attractive chassis for therapeutic protein delivery. However, its engineering has relied on a limited set of genetic tools and lacks systematic validation, which has constrained its development as a next generation engineered probiotic. In this study, the compatibility of the EasyClone-MarkerFree vector system with CRISPR-Cas9 editing in S. boulardii was systematically assessed. Eight genomic loci were identified in silico as suitable for integration, and four were used to construct strains with the Aspergillus niger lacA gene integrated at one or multiple genomic loci, encoding an extracellular β-galactosidase capable of hydrolysing lactose into glucose and galactose, an activity of potential therapeutic relevance for lactose intolerance. Engineered strains efficiently hydrolysed lactose, with the strain with the lacA cassette integrated in three genomic loci (Sb-Triple) displaying up to an 11.76-fold increase in specific β-galactosidase activity relative to single-integration strains, without compromising growth. Importantly, Sb-Triple retained the native pH tolerance characteristic of S. boulardii and was able to grow on, and hydrolyse lactose from, cheese whey powder, a food-grade substrate. These results demonstrate a validated CRISPR-Cas9 strategy for high extracellular β-galactosidase secretion in the clinically established S. boulardii CNCM I-745, while evaluating the compatibility of EasyClone-MarkerFree vector set with this strain. Overall, this work provides a foundation for future studies where the potential of the developed strains is evaluated in clinically relevant settings.