RESEARCH IN MICROBIOLOGY微生物学研究
RESEARCH IN MICROBIOLOGY(英文缩写 RES MICROBIOL),ISSN 0923-2508,eISSN 1769-7123,中文译名:微生物学研究 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-10-01 至 2026-09-30，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.946 | Q3 |
| 2022 | 2.600 | Q3 |
| 2023 | 2.500 | Q3 |
| 2024 | 3.400 | Q2 |
| 2025 | 4.400 | Q2 |
RESEARCH IN MICROBIOLOGY 最新收录文献
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1. Deletion of major shell proteins of ethanolamine utilization microcompartment reduces intrinsic antibiotic resistance, biofilm, and intracellular survival of Salmonella Typhimurium.
PMID:日期:2025-12-05With the high rise in Salmonella infections and emergence of antibiotic resistance, developing a novel strategy to control the pathogen is imperative. Earlier studies have revealed that ethanolamine (EA) metabolism plays a crucial role in Salmonella intestinal colonization; however, the potential of this metabolism as a therapeutic target remains unexplored. The EA metabolic enzymes are localized within a proteinaceous microcompartment (MCP) shell composed of thousands of copies of shell proteins encoded by five genes from the eut operon. Our study reveals that supplementation of EA and vitamin B in both rich and minimal media enhances biofilm formation, motility, and tolerance to antibiotics. Conversely, mutants deficient in EA metabolism due to defective MCP shell exhibited no physiological fitness. Fascinatingly, these mutants exhibited enhanced susceptibility to various antibiotics and lower expression of biofilm and curli. Also, a mutation in one of the major shell proteins reduced intramacrophage viability of Salmonella. Notably, phenotypes were restored upon ectopic expression of corresponding genes. Mutations in the MCP shell proteins downregulated the expression of genes related to pathogenicity. Overall, this study sheds new light on understanding the relationship between EA metabolism and bacterial physiology that would pave the way for developing novel therapeutic interventions against Salmonella.
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2. Dynamic exometabolomics reveals metabolic adaptations of Staphylococcus epidermidis to pH-mimicking skin and bloodstream.
PMID:日期:2025-12-03Staphylococcus epidermidis (SE) is a common human skin coloniser, which is often the cause of medical device-associated infections. SE population is composed of two clonal lineages, A/C and B, with distinct pathogenic potential. Although pH is known to change during infection when SE crosses the host skin to access the bloodstream, the impact of this pH alteration on SE pathogenicity is poorly understood. Recognizing how SE deals with pH increments will help designing effective prevention and treatment strategies against SE infections. To investigate the metabolic adaptations of representative A/C and B strains to pH, we mimicked skin and blood pH conditions (5.5 and 7.4) and followed biomass formation, growth media pH and exometabolites over time. Although both strains share some metabolic patterns, specificities were identified for each strain and pH condition. The B strain was better adapted to use diverse carbon sources and at blood pH has a more active TCA cycle and amino acid catabolism. At blood pH, the B strain depletes formate from the extracellular media, while its extracellular accumulation by the A/C strain could work as a host invasion strategy. For both SE strains, TCA cycle regulation, purine biosynthesis and glutamate uptake could be associated with virulence, particularly biofilm production, especially relevant for ICE25 which is able to produce high adherence biofilm. The uptake and consumption of saccharides follow similar profiles and seem to be pH-regulated by both strains. The dynamic study of SE exometabolome has contributed to understanding the intracellular processes and their relationship with virulence.
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3. Co-expression network insights into Leptospira interrogans pathogenesis.
PMID:日期:2025-11-19Leptospira interrogans, the agent of leptospirosis, employs complex virulence mechanisms that are not fully understood at a systems level. To elucidate the regulatory landscape of its pathogenicity, we used Weighted Gene Co-expression Network Analysis (WGCNA) on a comprehensive transcriptomic dataset to map the architecture of its virulence programs. Our analysis revealed that the L. interrogans transcriptome is organized into distinct, functionally coherent modules. We discovered that known virulence factors are significantly concentrated in two key modules: a "lightgrey" module that orchestrates host colonization and immune evasion, containing genes for surface adhesion (loa22, ompL1) and defense (lipL21); and a "black" module that functions as an arsenal for tissue invasion (colA), stress adaptation (clpB), and cytotoxicity (sph2). Furthermore, by contextualizing genes within this network, our approach implicated numerous uncharacterized genes (e.g., from the PF07598 family) in pathogenesis due to their strong co-expression with established virulence factors. These findings provide a systems-level blueprint of the regulatory networks driving leptospirosis, offering a rich resource of functionally validated gene modules and novel targets for the development of next-generation vaccines and therapeutics. These findings not only deepen the understanding of L. interrogans virulence regulation but also provide a conceptual framework for integrating transcriptomic data into systems-level models of bacterial pathogenesis, paving the way for translational applications in diagnostics and vaccine design.
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4. Differential DNA methylation associated with virulence attenuation in Edwardsiella piscicida.
PMID:日期:2025-11-14A marked difference in virulence was observed between two subpopulations of the Edwardsiella piscicida FSW910410 strain, originally isolated from olive flounder (Paralichthys olivaceus). While the strain maintained under routine laboratory culture conditions retained high virulence, the same strain stored long-term at -80 °C lost its ability to induce disease in olive flounder. Comparative in vivo assays demonstrated complete avirulence in the frozen-stored subpopulation, contrasting with high mortality rates induced by the virulent counterpart. Despite over 99 % genomic sequence identity and identical virulence gene repertoires, the two subpopulations differed significantly in their ability to evade host immune responses. Whole-genome methylation profiling revealed comparable levels of N6-methyladenine (mA) but notably reduced levels of cytosine methylation (mC/mC) in the avirulent subpopulation, particularly in genes associated with type III and type VI secretion systems (T3SS and T6SS). Quantitative RT-PCR analyses confirmed substantially lower expression of T3SS and T6SS genes in the avirulent strain. These results suggest that epigenetic modifications, especially cytosine methylation, may contribute to the observed differences in virulence, although additional regulatory mechanisms cannot be excluded. Collectively, the data highlight the complex interplay of genomic and epigenetic factors that may influence bacterial pathogenicity during prolonged storage or passage.
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5. Klebsiella pneumoniae-induced pneumonia: Pathogenesis, immune interactions, and antimicrobial resistance in a global context.
PMID:日期:2025-11-13Klebsiella pneumoniae is a Gram-negative bacterium and an important pathogen implicated in both hospital- and community-acquired pneumonia. K. pneumoniae strains are either classical, hypervirulent (hvKp), or antibiotic-resistant. There are several virulence factors of K. pneumoniae that helps immune evasion and survivability within the host. This review emphasizes the host immune interactions of K. pneumoniae. Evasion strategies of the pathogen and complex risk factors of the infection have been explained. Different diagnostic approaches, such as computed tomography, PCR, and Lateral Flow Immunoassay (LF), have been used for the diagnosis of K. pneumoniae. Multidrug-resistant Klebsiella pneumoniae (MDRKP) has emerged as a significant global public health concern, with higher cases of carbapenem-resistant K. pneumoniae (CRKP). CRKP caused more than 7000 deaths annually in Europe. Although K.pneumoniae has several mechanisms of antibiotic resistance, resistance to β-lactams, specifically carbapenems, causes a notable difference. Newer β-lactam/β-lactamase inhibitor combinations, such as ceftazidime-avibactam and cefiderocol are preferred for KPC-producing infections. This review emphasizes the ongoing challenges in translating therapeutic advancements into successful clinical outcomes, as well as the persisting complications in K. pneumoniae research, including the unsolved differences between hypervirulent and classical strains. Alternative methods, including bacteriophage therapy, antimicrobial peptides, and immunotherapy, are being studied for tackling CRKP. This review addresses recent advances in understanding Klebsiella pneumoniae-induced pneumonia, emphasizing how virulence factors interact with immune defences. Additionally, we highlight key challenges in antimicrobial resistance, current therapeutic strategies, K. pneumoniae's implications for infection control and antibiotic stewardship. Overall, this review aims to contribute to a deeper understanding of K. pneumoniae and to guide future interventions for effective prevention, control, and treatment strategies.
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6. Investigating inhibitory effect of sterol targeting compounds against B. anthracis: Membrane microdomain a probable target.
PMID:日期:2025-11-06This study aimed to evaluate the role of membrane microdomains (MMd) in virulence of Bacillus anthracis by assessing the effects of known antifungal compounds, referred to as raft-associated lipid biosynthesis inhibitors (RALBIs), on its pathophysiology. FDA-approved antifungal compounds representing three distinct classes-azoles (ketoconazole), allylamines (terbinafine), and polyenes (nystatin), were tested against B. anthracis. These compounds target different enzymes involved in MMd-associated lipid biosynthesis. Their impact on sporulation, toxin secretion, macrophage interaction, bio-signaling, cell envelope fluidity, and biofilm formation was examined. Additionally, their synergistic potential with conventional antibiotics was evaluated. RALBIs markedly attenuated the pathogenic traits of B. anthracis, including reduced macrophage association, diminished toxin secretion, and impaired sporulation. Treatment also altered growth kinetics, morphology, biofilm development and cell envelope fluidity. Importantly, the combination of RALBIs with erythromycin significantly reduced its minimum inhibitory concentration (MIC) against B. anthracis. These findings highlight membrane microdomains as crucial regulators of virulence in B. anthracis and identify RALBIs as promising adjunctive agents. By targeting MMd, sterol inhibitors disrupt multiple pathogenic pathways and enhance antibiotic efficacy, underscoring their potential as novel antibacterial strategies.
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7. Regulatory network of nucleotide second messenger in Pseudomonas aeruginosa PFL-P1 and intercellular signaling molecules for functional amyloid fibrillation in biofilm and stress response.
PMID:日期:2025-10-25Biofilm formation is governed by quorum sensing (QS) and intracellular signaling, with cyclic di-GMP (c-di-GMP) acting as a key regulator that modulates biofilm stability in response to environmental cues. The present study aims to explore the regulatory network between QS, c-di-GMP signaling, and amyloid production in the marine biofilm-forming bacterium Pseudomonas aeruginosa PFL-P1 under various physicochemical stressors. P. aeruginosa PFL-P1 demonstrated adaptability to diverse conditions typical of marine habitats, attributed to the activity of diguanylate cyclase (dgc86) and phosphodiesterase (pde94) genes regulating c-di-GMP turnover. Gene expression analysis revealed a coordinated regulatory network during biofilm development, with significant upregulation of dgc86, pde94, fapC (functional amyloid synthesis), lasI, rhlI (QS), and nahAc [polycyclic aromatic hydrocarbon (PAH) degradation] at 48 h, indicating a mature biofilm. Under acidic condition (pH 4), all genes except pde94 exhibited an adaptive response (p<0.0001). Salinity ≤1 % enhanced gene expression, whereas salinity ≥5 % suppressed it due to osmotic stress (p<0.0001). At 40 °C, dgc86 (p=0.0457) and fapC (p=0.0444) were upregulated, promoting biofilm stability. Pyrene exposure induced significant upregulation of dgc86, lasI, rhlI, and nahAc (p<0.05), enhancing biofilm formation and PAH degradation while downregulating pde94. Supplementation with C4-HSL and 3OC12-HSL upregulated these genes, reinforcing the role of QS in biofilm regulation. Terrein, a QS and c-di-GMP inhibitor downregulated fapC and nahAc, disrupting biofilm formation and PAH degradation. The strong correlation between c-di-GMP levels, amyloid production, and its high binding affinity to FapC (-11.8 kcal/mol) suggests a dual role for c-di-GMP as a signaling molecule and molecular chaperone in amyloid assembly.
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8. Epidemiology of anthrax in Andhra Pradesh.
PMID:日期:2025-10-22Over the years, anthrax has become endemic in the state of Andhra Pradesh, India, posing risk for both animals and humans and spreading to the bordering state of Karnataka. The incidence is on the rise and the spread covering the entire state. The article is aimed at projecting spatio-temporal distribution of anthrax in the state of Andhra Pradesh highlighting possible reasons influencing the transmission dynamics in animals and resultant risk to humans. A select sub-sample of these were analyzed by Whole Genome Sequencing. From 2009 to 2024, 122 animal anthrax outbreaks were recorded, whereas 21 human anthrax outbreaks were reported from 2004 to 2024 in the state of Andhra Pradesh. Animal anthrax outbreaks were reported throughout the year with short peaks during March to October, covering high summer and rainy season. Most affected species were sheep (84 %), followed by Bovines (9 %) and Goats (4 %). Spill over was detected in Pig and Hog deer, and a probable transmission in humans. The recent spread of anthrax in livestock across the state, particularly to adjoining pasture lands of Odisha, Karnataka and Tamil Nadu due to migration for grazing might have led to the recent hot spots around state boundaries and forest fringes, calls for effective intervention to stop the spread.
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9. An electron microscopy study of Bacillus anthracis capsule reveals early expression and novel strand-like structures.
PMID:日期:2025-10-10Capsule is a key virulence factor for Bacillus anthracis. In this study, we examined the onset of capsule expression and the ultrastructure of capsule expressed in vitro and in infected hosts. We determined by immunofluorescence and immunoelectron microscopy that capsule was produced as early as 15 min after onset of germination. Further analysis revealed bacilli were often fully encapsulated when they emerged from the exosporium. Using conventional fixative to prepare samples for electron microscopy resulted in loss of capsular material, but using a fixative containing ruthenium red and lysine acetate preserved it. Transmission electron microscopy using enhanced fixation revealed that B. anthracis grown in vitro were surrounded by a thick capsule layer comprised of globular structures and a slime layer. When examined using the enhanced fixation, extracellular bacilli in tissues from moribund animals were surrounded by a thick capsule layer, and had long branching strands like those observed on live but not fixed bacilli by fluorescence microscopy. Scanning electron microscopy using enhanced fixation similarly revealed B. anthracis within spleen sinusoids were covered with long branching strands that appeared to tether them to host cells. This is the first report describing the presence of long capsule strands during infection in vivo.
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10. Molecular analysis of Bacillus anthracis isolates from Karnataka's ruminant anthrax outbreaks reveals genetic relationships and environmental factors influencing spore persistence.
PMID:日期:2025-10-04Anthrax outbreaks continue to be reported from several districts of Karnataka in India. Many outbreaks in livestock and humans have reported, the soil and climatic conditions would have supported long-term spore persistence. Hence, the state of Karnataka in India was chosen as an initial pilot site to study, isolate, and characterize Bacillus anthracis. In this study, we integrate molecular typing and soil analysis across endemic and non-endemic areas, an approach that has been scarcely explored and could be a novel contribution. By comparing both endemic and non-endemic regions, it would be an opportunity to examine soil-related ecological factors that could have been linked to the anthrax outbreaks. A total of 45 suspected animal anthrax cases were investigated using culture and PCR-based methods. Colonies with Medusa head-like appearance on blood agar plates were subjected to PCR assays targeting protective antigen (pXO1), capsular gene (pXO2), and the chromosomal rpoB genes. Additionally, soil samples from 12 different sites (six endemic and six non-endemic) were processed using the Ground Anthrax Bacillus Refined Isolation (GABRI) method, which was used to enhance the detection of B. anthracis spores by reducing environmental contaminants that may inhibit spore germination and growth. Out of 45 suspected animal anthrax cases, 09 isolates were confirmed as Bacillus anthracis with colony characteristics of greyish-white, frosted-glass on blood agar, further verified through PCR analysis. Phylogenetic analysis based on the rpoB gene demonstrated a close genetic relationship among these isolates, suggesting that the transmission of spores was localized and likely facilitated by animal movement. The soil analysis showed that the endemic sites had an alkaline pH of 7.81-8.9, higher organic carbon 0.45-4.36 %, elevated phosphorus 10.32 to 123.7 kg/ha, and greater clay content up to 45 % in Bellary, contributing to higher survivability and retention of spores in endemic regions. In contrast, non-endemic soil exhibited neutral to slightly acidic pH (6.1-6.85), lower phosphorus levels, and sandy clay loam texture that may limit long-term spore retention. The study offers significant insights into the environmental and genetic factors contributing to the persistence of Bacillus anthracis in both endemic and non-endemic regions. The finding shows that soil plays a crucial role in the survival and transmission of anthrax spores, with higher retention observed in endemic areas. The close genetic relationship among isolates further suggests localized transmission, likely influenced by animal movement. These results underline the need for continued surveillance and prevention strategies, especially in regions with a history of anthrax outbreaks.