GENOME基因组
GENOME(英文缩写 GENOME),ISSN 0831-2796,eISSN 1480-3321,中文译名:基因组 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-10-01 至 2026-09-30，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.449 | Q3 |
| 2022 | 3.100 | Q2 |
| 2023 | 2.300 | Q3 |
| 2024 | 1.700 | Q3 |
| 2025 | 1.900 | Q3 |
GENOME 最新收录文献
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1. Integration of cytogenetics and genomics reveals translocation and recombination between nonhomologous chromosomes in a novel wheat line with dual introgression.
PMID:日期:2026-10-03The development of wheat-rye-Thinopyrum hybrids is a key strategy for introgressing agronomically valuable traits from multiple genera into common wheat, enriching its genetic diversity. However, the labor-intensive and time-consuming identification of lines carrying chromatin segments from all three donors has limited its widespread application. In this study, a stable hexaploid wheat line, 13-26 (2n=6x=42, AABBDD, T6JvsS·6AL-T5R·2D-T5R·4B), was developed by crossing octoploid Trititrigia with hexaploid triticale, followed by 17 generations of self-pollination. Line 13-26 outperforms its parents agronomically, with well-filled kernels and a TKW exceeding both parental lines, and the higher protein content relative to the male parent. Analyses via smGISH, mcFISH, and short-read sequencing confirmed the integration of wheat, rye, and Thinopyrum chromatin. Approximately 220 Mb was absent from wheat chromosome 6AS and 89 Mb from 2DL, while around 221 Mb of Thinopyrum 6JvsS and 42 Mb of rye 5RL were detected. Line 13-26 carries a Th. intermedium T6JvsS·6AL translocation and terminal segments of rye 5RL translocated to wheat 2DL and 4BL, demonstrating intergeneric translocation and recombination between nonhomologous chromosomes, which represents a valuable germplasm resource with integrated multi-genus traits. This combined cytogenomic approach enhances the sensitivity and specificity of alien chromatin detection, providing a robust framework for characterizing complex chromosomal rearrangements in polyploid wheat.
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2. Identification and functional analysis of 2,3-dihydroxybenzoic acid and catechol catabolic pathway genes in Aspergillus niger.
PMID:日期:2026-09-14Microorganisms catabolize many plant-derived aromatic compounds through the β-ketoadipate pathway. This pathway has two branches, beginning with protocatechuic acid and catechol, which converge on β-ketoadipate before further catalysis to produce tricarboxylic acid cycle intermediates. The protocatechuic acid branch of the pathway has been characterized in fungi.Here, we characterized the genes of the catechol branch of the β-ketoadipate pathway in the filamentous fungus Aspergillus niger. We predicted the genes involved using comparative transcriptomics and homology to characterized genes. We tested the predictions by constructing knockout mutants lacking candidate genes to observe the growth phenotype on 2,3dihydroxybenzoic acid (2,3-DHB), which is catabolized through the catechol branch, and by detection of metabolites accumulated in knockout mutants using mass spectrometry. Using these approaches to reconstruct the pathway, we confirmed the reported assignments of dhbA, crcA, kstA, and kctA as encoding 2,3-dihydroxybenzoate decarboxylase, catechol 1,2dioxygenase, β-ketoadipate:succinyl-CoA transferase, and β-ketoadipyl-CoA thiolase, respectively. We further assigned mciA, mliA, and kelA as encoding muconate cycloisomerase, muconolactone isomerase, and β-ketoadipate enol-lactonase, respectively. Additionally, we identified a transcription regulator involved with the β-ketoadipate pathway in fungi for the first time. This gene, pcaR, is upregulated on 2,3-DHB and deletion of the gene impaired growth on 2,3-DHB.
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3. Coffea Comparative Genomics Reveals Subgenome-Associated Expansion and Diversification of Biosynthetic Gene Clusters.
PMID:日期:2026-08-18Biosynthetic gene clusters (BGCs) are important for plant specialized metabolism, but remain poorly characterized in coffee. Given Brazil's importance in coffee production, we performed a comparative genomic analysis of BGCs across the allotetraploid Coffea arabica and its diploid progenitors C. eugenioides and C. canephora. Using standardized genome filtering, annotation, orthogroup inference, and cluster classification, we identified 472 BGCs comprising 3,118 biosynthetic genes, which were grouped into 194 cluster families and integrated with 28,280 orthogroups. Of these, 7,091 orthogroups were shared across all species; Coffea canephora and subgenomes shared 10,923, while Coffea eugenioides and subgenomes shared 11,446. Most BGC-associated orthogroups (86.4%) link to a single pathway class. BGC-associated genes form a highly structured yet lineage-dynamic component of the Coffea pangenome. C. eugenioides and its derived subgenomes in Arabica contributed disproportionately to 14 BGC-associated orthogroups, including flavonoid-, lipid-, and stress-related functions. In contrast, C. canephora derivatives contributed only two terpene-related orthogroups. The parental species showed fewer secondary metabolism-related enriched GO terms (3 and 1) than their subgenomes (53 and 56). Species-specific rearrangements, expansions, and subgenome retention indicate that hybridization and polyploidy shaped BGC diversification. These results advance understanding of specialized metabolism in Coffea and identify targets for coffee improvement and climate resilience.
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4. Filling DNA barcode gaps in Central European Auchenorrhyncha: a new reference library and Oxford Nanopore Technologies-based species identification.
PMID:日期:2026-08-14Terrestrial arthropods are declining in most ecosystems, creating a need for efficient and scalable biodiversity monitoring tools. DNA-barcoding enables high-throughput species identification but is constrained by incomplete reference libraries for many taxa. Here, we expand the DNA barcode database for Austrian Auchenorrhyncha by combining morphological identification with Sanger-sequenced COI barcodes. A total of 1 304 specimens representing 394 species were successfully barcoded, resulting in 384 Barcode Index Numbers (BINs), including 73 newly established BINs. Elevated intraspecific COI divergence and BIN discordance in several taxa reveal species complexes and missing barcode gaps. The expanded reference library was applied to Auchenorrhyncha from Malaise trap samples collected at an agricultural lowland and an alpine site and analyzed using a high-throughput Oxford Nanopore Technologies (ONT) workflow. The improved reference library substantially increased assignment success and taxonomic resolution. Finally, we compared the ONT barcoding results from Malaise trap catches with morphology-based identifications from single-event suction sampling. Both approaches detected similar proportions of species, and differences in species composition likely reflect sampling- and method-specific biases. Overall, this study advances the development of a comprehensive DNA barcode reference library for Central European Auchenorrhyncha and underscores the importance of regionally curated databases and complementary approaches for biodiversity assessments.
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5. {"_":"Abiotic stress-responsive tRNA-derived fragments in pitanga ( L.): regulatory roles in drought and salinity adaptation.","i":["Eugenia uniflora"]}
PMID:日期:2026-06-15The tRNA-derived RNA fragments (tRFs) have emerged as pivotal regulators of eukaryotic stress responses. These small non-coding RNAs are implicated in various biological domains. This study investigates the expression and functions of plant tRFs in response to abiotic stress with focus on Eugenia uniflora L. In silico analysis identified a diverse set of small RNAs originating from 73 conserved tRNAs. High-throughput analysis of small RNA (sRNA) libraries revealed a prevalence of 5' tRFs, with 51,947 sRNAs. RT-qPCR analysis unveiled predominant tRFs, such as those derived from tRNA-Arg-CCT. Among the 11 tRFs examined, six displayed notable differences in expression levels under high salt and osmotic stress triggered by NaCl and polyethylene glycol. Notably, 5'tRF ArgTCG and 5'tRF GlyTCC were positively regulated under both saline and water stress conditions. Additionally, we demonstrated that some previously identified targets of these tRFs showed reduced expression following both types of stress. Our findings suggest that abiotic stress-related tRFs interact with various signaling and metabolic pathways. Alterations in the expression of three tRFs and their target genes in both stress conditions highlight the potential significance of tRF changes in E. uniflora adaptation to drought and salinity.
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6. Diacylglycerol kinase promotes forgetting of aversive olfactory memory in Drosophila.
PMID:日期:2026-06-15Decades of neuroscience research using the Drosophila model system has made enormous strides in uncovering genes that positively support the ability to form new memories or consolidate them for the long-term. However, recent research has revealed the existence of more rare but equally important memory suppressor genes whose normal function is to limit memory formation or even promote active forgetting. Here we report that RNAi targeting of Diacylglycerol kinase (Dgk), a major regulator of lipid signaling, within the mushroom body memory circuitry specifically enhances aversive olfactory memory retention while leaving learning and sensory-motor behavioural controls unaffected. Furthermore, memory retention is enhanced when targeting Dgk in these circuits with two additional RNAi lines. Finally, using in vivo functional imaging, we offer evidence that Dgk plays a role in regulating baseline synaptic transmission of memory circuits. While the exact mechanism for Dgk's synaptic effects and the implications for memory storage remain unclear, our findings implicate lipid signaling via Dgk as an important regulator of active-forgetting pathways. This work builds on our prior understanding of the importance of lipids in cognition by extended their role to memory suppression and active forgetting.
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7. From Lab Bench to Public Voice: How to Incorporate Science Communication into Your Research Program.
PMID:日期:2026-06-01Science and research are under attack and scientists need to fight back. Public access to information is at an unprecedented high, but misinformation and disinformation are rampant and societal trust in science and research is at a historic low. Scientists need to be part of restoring trust in science and evidence-based decision making. For a variety of reasons, including time constraints, little professional recognition, and limited communication experience, too few scientists are involved in science outreach. Here, based on our experience as research scientists who engage in science outreach, we suggest ways to address these issues including four "How-To's": Take the leap, Engage, Find Partners, and Explain the scientific process. Like scientific research, science communication is a craft that can be intentionally practiced and developed. Scientists can restore trust in science and research. It is in our best interest, and that of society as a whole, for us to step up and meet this challenge.
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8. Systematic evaluation of rDNA ITS2 as a DNA barcode for molecular identification of cyclorrhapha flies in China.
PMID:日期:2026-01-01Morphological identification of medically important flies is often hindered by incomplete or damaged specimens, cryptic species, and morphological conservatism among closely related species. In this study, common fly species were collected from 15 cities across 10 provinces in China to evaluate rDNA internal transcribed spacer 2 (ITS2) as a potential DNA barcode for molecular identification. A total of 1252 fly specimens were collected, of which 933 were morphologically classified into 19 species, 15 genera, 9 families, and 3 superfamilies; 319 remained unidentified due to the loss of key morphological characters. Seventeen mitochondrial and ribosomal candidates were evaluated using sequences retrieved from GenBank across Cyclorrhapha. ITS2 was selected based on its relatively low intraspecific divergence, higher interspecific divergence, and conserved flanking regions suitable for universal primer design. The designed ITS2 primers successfully amplified and sequenced all 144 tested specimens, including morphologically damaged samples. Integrating morphological and molecular data, 27 fly species were identified. Barcode gap analyses indicated clear interspecific discrimination in the genera of Muscidae, Calliphoridae, and Sarcophagidae. Phylogenetic analyses further supported species-level identification and revealed ITS2 sequence diversity within specimens. These results demonstrate that ITS2 is a promising candidate DNA barcode for Cyclorrhapha flies, particularly for specimens with ambiguous or damaged morphology.
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9. {"_":"Unraveling evolutionary relationships in the generic alliance (Malvaceae, Malvoideae): a phylogenetic and cytotaxonomic overview.","i":["Sida"]}
PMID:日期:2026-01-01(Malvaceae), the largest Malveae-Abutilinae member, has poorly defined morphological limits which overlaps with 11 phylogenetically closely related genera that comprises the " generic alliance". The 12 genera are distributed in the tropics especially in Brazil where one third of its species diversity is found. Evolutionary relationships within generic alliance remain unresolved due to morphological convergence, limited taxon sampling, and lack of integrative approaches including cytogenetic data. We reconstructed the phylogeny of and allied genera using a multilocus dataset (nuclear and seven plastid loci) including 193 species classified in 19 genera and analyzed chromosome evolution using cytogenetic data (chromosome number) for 79 species of the 19 genera. The phylogeny recovered seven clades-, and three clades (I-III)-and confirmed the polyphyly of , the largest genera. We detected reticulate evolution, with incongruence between nuclear and plastid topologies. Chromosome number ranged from 2 = 12 to 60 and represented synapomorphies for most clades. Ancestral character reconstruction indicated that ascending dysploidy and polyploidy predominated in karyotype evolution of and allied genera. Our results reveal taxonomic incongruence in current classifications probably related to reticulate evolution. A generic-level taxonomic revision is necessary and should rely on integrated phylogenetic and karyotypic evidence. This study provides a framework for phylogenetic systematics and emphasizes the role of Brazil as a hotspot for plant genomic research.
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10. Cytogenetic diversity in maize landraces shaped by long-term cultivation and ecogeographic adaptation.
PMID:日期:2026-01-01This study characterizes the cytogenetic diversity of maize landraces from Northern Argentina, a region comprising Northwestern Argentina (NWA) and Northeastern Argentina (NEA). New data from four NWA landraces were analyzed using DAPI banding, fluorescence in situ hybridization, and flow cytometry. Intra- and inter-populational variation was detected in heterochromatin content (2.06%-12.6%), as well as in the number (3-15), size, and chromosomal position of knobs. Genome size (2C value) ranged from 5.27 to 5.74 pg, and numerical polymorphism (0-2) of B chromosomes was observed. Consensus idiograms were constructed for each studied landrace. A quantitative analysis combining these data with previously published information on Argentine maize revealed a negative association between knob heterochromatin content and cultivation altitude. In NWA landraces, genome size was also negatively associated with altitude, whereas no such relationship was detected when NWA and NEA data were analyzed together. In NEA landraces, which grow within a narrow altitudinal range, genome size variability may be influenced by local farmers selection practices, whereas in NWA landraces cytogenetic variation may reflect multifactorial anthropogenic and environmental influences. B chromosomes were recorded exclusively at altitudes above 2000 m.a.s.l. The observed cytogenetic patterns are discussed in the context of ecogeographic differentiation among maize landraces.