PLANT MOLECULAR BIOLOGY植物分子生物学
PLANT MOLECULAR BIOLOGY(英文缩写 PLANT MOL BIOL),ISSN 0167-4412,eISSN 1573-5028,中文译名:植物分子生物学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.335 | Q1 |
| 2022 | 5.100 | Q1 |
| 2023 | 3.900 | Q1 |
| 2024 | 3.800 | Q1 |
| 2025 | 3.700 | Q2 |
PLANT MOLECULAR BIOLOGY 最新收录文献
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1. Overview of molecular mechanisms underlying stress responses; signaling pathways and secondary metabolite synthesis in plants.
PMID:日期:2026-09-21Plants are susceptible to several biotic and abiotic stressors, including pathogens, extreme salinity, temperature, and drought. Continuous exposure to these conditions activates complex molecular mechanisms in plants. These include stress-signaling pathways mediated by phytohormones such as cytokins, salicylic acid, auxins, abscisic acid, jasmonic acid, and ethylene. These signaling molecules further interact with cellular pathways regulating stress-responsive gene expression through transcription factors such as DREBs, MYBs, bZIPs, and NACs. Key cellular pathways include mitogen-activated protein, calcium-dependent protein, and receptor-like kinases. Under continuous stress signaling, plants adopt adaptive strategies, including increased biosynthesis of secondary metabolites that protect against oxidative stress, pathogens, herbivores, and environmental stress. Major secondary metabolites include alkaloids, flavonoids, terpenoids, phenolic acids, and saponins. These molecules modulate redox homeostasis and activate antioxidant defense systems, including superoxide dismutase, catalase, and peroxidase. Advanced genomic and molecular tools reveal complex interactions between secondary metabolite synthesis and stress signaling. This suggests a novel approach to enhancing plant stress tolerance and improving crop resilience through genetic manipulation of these pathways. Given the complexity of these pathways, integrated system biology approaches are highly useful. Therefore, this review aims to explore the molecular mechanisms controlling plant stress regulation and their relationship to secondary metabolite biosynthesis. This review further proposes integrative system-biology approaches to enhance stress tolerance and to produce bioactive compounds for agricultural and pharmaceutical applications.
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2. Stage-preferential transcriptome profiling reveals senescence associated transcriptional programs linked to nutrient remobilization in rice flag leaves during grain filling.
PMID:日期:2026-09-19Leaf senescence during grain filling in cereals redistributes carbon, nitrogen, and mineral nutrients from source leaves to developing grains, thereby strongly affecting yield formation. Here, we profiled rice flag leaf transcriptomes at five reproductive stages (1 week before heading, heading, and 1, 3, and 5 weeks after heading) and identified stage-preferential genes using an intersection-based criterion in which genes upregulated at one stage were compared with those at all other stages. Transcriptional reprogramming was most pronounced at heading, with 419 preferentially expressed genes, and at five weeks after heading, with 1317 genes. Genes preferentially expressed at heading were enriched in jasmonate-related and defense-associated processes, whereas genes preferentially expressed at five weeks after heading were associated with senescence, nutrient transport, alternative respiration, and plastid regulation. A STRING-guided protein-protein interaction network integrating genes preferentially expressed at five weeks after heading with reference genes from the Leaf Senescence Database identified hub candidates associated with chloroplast and chlorophyll turnover and nitrogen remobilization. Quantitative RT-PCR analysis in the pale-green leaf mutant (pgl) revealed broadly reduced expression of representative hub genes, suggesting attenuation of late-stage senescence network activity. Together, these findings provide a stringent stage-resolved framework for prioritizing regulators of late senescence in rice flag leaves during grain filling.
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3. γ‑polyglutamic acid���induced GhJAZ8 positively regulates drought tolerance in cotton associated with phytohormone signaling.
PMID:日期:2026-09-17Cotton growth is frequently constrained by drought stress. Previous studies demonstrated that γ-PGA enhances cotton drought tolerance. Transcriptome analysis of γ-PGA-treated cotton revealed that multiple TIFY family members were markedly induced, among which GhJAZ8 showed the most pronounced upregulation. Sequence analysis indicated that GhJAZ8 belongs to the JAZ/TIFY5A subfamily, and its expression was significantly upregulated under drought stress. Virus-induced gene silencing of GhJAZ8 markedly decreased drought tolerance in cotton, evidenced by aggravated leaf wilting, reduced RWC, elevated MDA content, weakened antioxidant enzyme activities, and decreased proline accumulation. Conversely, overexpression of GhJAZ8 in Arabidopsis significantly enhanced drought tolerance. Furthermore, γ-PGA treatment improved drought tolerance in both TRV2 and GhJAZ8-silenced plants, but the effect was more pronounced in TRV2 plants, indicating that GhJAZ8 is required for the full effect of γ-PGA. Exogenous MeJA treatment only partially alleviated the drought-sensitive phenotype of GhJAZ8-silenced plants. Transcriptome analysis revealed that DEGs were predominantly enriched in ABA, JA, and SA signaling pathways, and hormone quantification further confirmed that GhJAZ8 silencing significantly altered endogenous ABA, JA, and SA levels. Taken together, this study reveals that GhJAZ8 functions as a positive regulator in cotton drought response and acts as an important downstream component of the γ-PGA-mediated drought tolerance pathway by modulating multiple phytohormone signaling pathways, providing a candidate gene resource for drought-tolerant cotton breeding.
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4. Pan-genome characterization of the maize 4CL gene family and its dynamic responses to abiotic stress.
PMID:日期:2026-09-101.Pan-genome analysis across 26 maize inbred lines identified 13 Zm4CL genes (nine core and four near-core) classified into three evolutionary clades.2.Structural variations (SVs) are significantly associated with the expression and altered conserved protein domains of key Zm4CL genes.3.Zm4CL genes exhibit distinct tissue-specific expression patterns and dynamic enzymatic and transcriptional responses to stresses, particularly cold and drought.4-Coumarate:CoA ligase (4CL) is a key enzyme in the phenylpropanoid pathway and plays important roles in plant growth, development, and responses to environmental stresses. However, a comprehensive pan-genome analysis of the 4CL gene family in maize is still lacking. In this study, 13 Zm4CL genes were identified from a maize pan-genome comprising 26 diverse inbred lines, including nine core genes and four near-core genes. Phylogenetic analysis classified these genes into three evolutionary clades, while Ka/Ks analysis indicated that most members have been maintained under purifying selection, although several genes exhibited greater evolutionary divergence and relatively relaxed evolutionary constraints. Structural variation (SV) analysis revealed significant associations between SVs and the expression of Zm4CL2 and Zm4CL3, while sequence comparisons suggested that SVs were also associated with alterations in conserved protein domains in some genotypes. Transcriptome analyses revealed distinct tissue-specific expression patterns and diverse transcriptional responses to abiotic and biotic stresses. Enzyme activity assays showed that cold stress significantly increased 4CL activity at 12 h, whereas heat, salt, and alkali stresses caused an initial decrease followed by recovery, while drought had no significant effect. Time-course RT-qPCR further validated dynamic expression changes of representative Zm4CL genes under cold and drought stresses. Overall, this study provides a comprehensive pan-genome framework for understanding the evolutionary conservation, regulatory diversification, and stress-responsive characteristics of the maize Zm4CL gene family, providing valuable resources for future functional studies and the genetic improvement of stress tolerance in maize.
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5. Pinpointing genomic regions conferring herbicide tolerance in cassava via genome-wide association mapping.
PMID:日期:2026-09-09Cassava (Manihot esculenta Crantz) is a tropical crop of major socioeconomic importance, whose productivity can be limited by sensitivity to herbicides used for weed management. This study aimed to perform a genome-wide association study (GWAS) in 194 cassava genotypes to identify genomic regions associated with tolerance to the herbicides mesotrione, S-metolachlor, and chloransulam-methyl. The evaluations performed at 3, 6, 9, 15, and 30 days after application (DAA) were used to characterize the temporal progression of phytotoxicity. Based on this analysis, the phenotype obtained at 9 days after application (PhytoX9DAA) was selected for genome-wide association analyses because it represented the period of greatest symptom expression and the highest discrimination among genotypes. GWAS analyses were performed using de-regressed BLUPs and the MLM, MLMM, and BLINK models, incorporating kinship (K) and population structure (Q) matrices. Significant markers were detected across multiple chromosomes, and the corresponding genomic windows contained candidate genes with functional annotations related to herbicide response. The predominant functional categories included membrane transport, channel activity, signal peptide processing, protein phosphorylation, cellular signaling, and metabolic regulation. Key candidate genes included Manes.02G151900 and Manes.02G152700 (chromosome 2), associated with transmembrane transport and signal peptide processing; Manes.09G060900 (chromosome 9), associated with protein kinase activity, ATP binding, and protein phosphorylation; and Manes.15G083800 and Manes.15G084000 (chromosome 15), associated with S-adenosylmethionine-dependent methyltransferase activity, membrane-related functions, and protein phosphorylation. These genes participate in biochemical pathways involved in cellular signaling, membrane transport, and metabolic regulation that may contribute to herbicide tolerance. Overall, the results demonstrate that herbicide tolerance in cassava is a quantitative and polygenic trait governed by numerous small-effect loci. The integration of cellular signaling, metabolic regulation, and membrane transport supports the physiological resilience of the species under chemical exposure, providing valuable insights for breeding strategies and marker-assisted selection.
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6. Loss of galactan synthesis in pea (Pisum sativum) causes defects in organ expansion and is associated with increased extensin content.
PMID:日期:2026-09-09Pectic galactan is a rhamnogalacturonan-I side chain, composed of β-(1→4)-linked D-galactose residues. Its multifunctional role in cell wall assembly and properties has been proposed in various developmental contexts. In this study, we investigated the role of galactan in pea (Pisum sativum syn. Lathyrus oleraceus). First, we used glycan profiling to examine stems with different auxin-induced elongation rates for changes in cell wall composition. The galactan epitopes, recognised with the LM5 monoclonal antibody, were more abundant in the convex, more elongated part of the pea stem. This finding was supported by monosaccharide composition analysis and epitope detection chromatography (EDC). To evaluate the impact of disrupting galactan synthesis on pea development, we used Virus-Induced Gene Silencing (VIGS) to downregulate the auxin-inducible pea ortholog of Arabidopsis β-1,4-galactosyltransferases of the GT92 family (PsGALS3). Plants with silenced PsGALS3 exhibited severe defects in elongation and vegetative organ growth. Microarray polymer profiling (MAPP) revealed that, alongside the reduction in galactan epitopes, the cell wall proteoglycan extensin content increased in these plants, as confirmed by in situ immunohistological analysis. Our results provide new evidence linking galactan to cell and organ expansion.
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7. PlantCCC prioritizes context-specific candidate ligand-receptor communication patterns in plant spatial transcriptomics.
PMID:日期:2026-09-07Intercellular communication supports plant development and environmental responses, but its analysis in plant tissues is complicated by cell walls, plasmodesmata, and local tissue architecture. Spatial proximity therefore does not necessarily indicate effective communication. Plant ligand-receptor (L-R) resources also contain expanded gene families, homology-derived mappings, and uneven levels of experimental support. We developed PlantCCC, a spatially aware graph-learning framework that uses a plant L-R database as a candidate search space and combines residual spatial expression enhancement, a directed heterogeneous candidate graph, expression-gated spatial weighting, spatially aware multi-head graph attention, and self-supervised contrastive learning to prioritize context-specific candidate edges. In a semi-synthetic benchmark, PlantCCC distinguished TRUE pairs containing an injected interaction component from CONFOUNDER pairs showing tissue co-localization alone, and remained comparatively robust under dropout perturbation. In poplar stem analyses based on a homology-derived Populus candidate L-R set, and in an independent Arabidopsis Visium HD analysis based on Arabidopsis PlantPhoneDB entries, PlantCCC prioritized candidate L-R axes that were consistent with tissue architecture, spatial expression patterns, and prior evidence for the corresponding signaling modules. PlantCCC provides an interpretable computational framework for prioritizing context-specific candidate cell-cell communication patterns in plant spatial transcriptomics.
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8. Detailed re-analysis of satellitome mapping facilitated by the telomere-to-telomere (T2T) assembly of bread wheat genome.
PMID:日期:2026-08-28The satellitome of Triticum aestivum cv. Chinese Spring consists of 36 satellite DNA (satDNA) families that were previously molecularly characterized and chromosomally mapped. The recent release of a complete gap-free telomere-to-telomere (T2T) assembly of the species offers a unique opportunity to refine the physical map and evolutionary interpretation of these sequences. Here, we re-evaluate the organization of the wheat satellitome by analyzing the T2T assembly with RepeatMasker and visualizing the results using the CHRISMAPP script. This research strengthens the notion that more than half of wheat satDNAs are connected to transposable elements (TEs) and enables us to propose a model of satDNA origin and evolution driven by TEs and other random genome sequences. By integrating T2T based physical mapping with previous FISH analyses, we refine the genomic distribution of tandem repeats, identify complex satellite organizations and length variants, and resolve several discrepancies between cytogenetic and genomic analyses. Finally, the detailed mapping of satDNA in centromeric and the subtelomeric regions highlight clear chromosome and subgenome specific patterns, suggesting potential roles of satellites in chromosome architecture. Together these findings provide the most complete and accurate satellitome map available for bread wheat, offering new insights into repeat evolution and genome organization in polyploid species.
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9. Characterization of disomic alien addition lines from an intersubgeneric cross between Glycine max and G. tomentella and phenotypic variation within those lines and their progenies.
PMID:日期:2026-08-28Disomic alien addition lines (DAALs, 2n = 42) were obtained from an intersubgeneric cross between Glycine max [L.] Merr. cv. Dwight (2n = 40, G1G1) and Glycine tomentella Hayata (PI 441001, 2n = 78, D3D3CC). These DAALs had two homologous G. tomentella chromosomes. In some progenies of the DAALs the extra G. tomentella chromosomes were eliminated. One objective of this research was to identify differences in gene expression between a DAAL (LG13-7552) and one of its 2n = 40 progenies (LG12-7063). RNA-sequencing documented that many genes critical to fundamental plant growth and related to stress and defense responses were differentially expressed. DNA and RNA-sequencing data showed that the gray pubescence of LG12-7063 was due to a novel adenine deletion in the T locus. When the DAALs were grown in the field, progeny were identified that possessed characteristics that were absent in both parents. Additional objectives of this research were to document this phenotypic variation and to determine the cause of these changes. All off-type progenies lost their G. tomentella chromosomes and reverted to the normal soybean chromosomal state (2n = 40). Significant variation was observed among the 2n = 40 progenies for the several qualitative and quantitative traits. Field research documented an average reversion frequency within the DAAL rows of 4%. Genotypic data showed that the revertants (2n = 40) differed from the sister DAAL lines by mostly heterozygous polymorphisms throughout the genome.Natural cross-pollination was the most probable cause of observed variation but the reasons for the elevated level of cross pollination and the mechanisms for long distance pollen transfer remain unknown.
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10. Molecular cartography of root nodule organogenesis: insights into the role of transcription factors.
PMID:日期:2026-08-27Nodule organogenesis is a complex developmental process which is induced post-embryonically following rhizobia infection and relies on coordinated action of a suite of transcription factors at multiple stages starting from its initiation, de novo meristem establishment and differentiation. The rewiring of nitrate-responsive NIN-LIKE PROTEIN (NLP) homolog, NODULE INCEPTION (NIN) transcription factor through genetic adaptations in the common ancestor of the nitrogen-fixing clade is crucial to the origin of nodulation trait. Moreover, nodule formation emerged through the co-option of evolutionary ancient root/lateral root developmental pathways mediated by conserved transcriptional regulators such as NF-Y, LBD16, SHR/SCR, WOX5, PLT and KNOX. Importantly, NIN has acquired functions to integrate signals from rhizobia infection and divert the existing developmental pathways towards nodule organogenesis, thereby acts as a master regulator of root nodule symbiosis. Nodule specific innovations in the existing developmental pathway genes through mechanisms like genetic adaptations in their cis-regulatory region, paralog retention, changes in spatio-temporal gene expression pattern and functional changes to the protein are crucial for imparting developmental novelty during nodule organogenesis. Here, we provide a consolidated idea on transcription factor-mediated genetic modules regulating distinct stages of nodule organogenesis with an emphasis on their nodule specific innovations. This knowledge gain is particularly important to engineer nitrogen-fixing nodules into non-nodulation crop plants which will eventually minimize the reliance on synthetic nitrogen fertilizers and thereby favors a sustainable agricultural system.