Advances in Experimental Medicine and Biology实验医学与生物学进展
Advances in Experimental Medicine and Biology,ISSN 0065-2598,eISSN 2214-8019,中文译名:实验医学与生物学进展 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2020 | 2.622 | Q2 |
| 2021 | 3.650 | Q2 |
Advances in Experimental Medicine and Biology 最新收录文献
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2. The Emergent Architecture of Autonomous Clocks Described by Computational Algorithms of Regulatory Networks.
PMID:日期:2026-01-01Autonomous oscillators in systems biology are often mathematically expressed as regulatory network models. These models are interrogated to predict oscillator behavior under various environmental conditions. The potential suite of dynamical behaviors arising from a regulatory network model is rich and varied, as one might expect of a complex system. Therefore, a mathematical model has the capability to predict previously unobserved biological oscillator behavior that arises under untested conditions. We present a computational method that describes the full suite of dynamical behaviors of a network model, enabling both mechanistic understanding of experimental observations and prediction of unexpected dynamics. These ideas are presented through example network models, including some relevant to the yeast cell cycle.
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3. Physical Frameworks and Theories on How Autonomous Biological Oscillators Synchronize.
PMID:日期:2026-01-01Rhythms and oscillations are widely observed in biological systems. However, it is unclear how such noisy systems can produce robust and consistent timing, or how individual molecules or time-keeping systems couple together to produce macroscopically observable rhythms. Here, we focus on the synchronization of oscillatory processes at different scales: from the sub-cellular to the cellular and intercellular levels. Using examples from the circadian rhythms in cyanobacteria, cell cycle in eukaryotes, the firing of neurons, and from other emerging findings on the frontier of autonomous clocks, we discuss different paradigms of coupling or entrainment. We then highlight different methods of analysis from deterministic to stochastic approaches. Specifically, we discuss methods to quantify synchronization and its possible physical mechanisms, as well as prevailing mathematical models. Lastly, we close with a discussion of suggestions for new experiments or theoretical investigation.
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4. Leveraging Autonomous Biological Oscillators for Directed Evolution of Switchable Proteins.
PMID:日期:2026-01-01Engineering proteins that can switch between states is of strong interest to biotechnology. These include multi-state transcription factors, membrane receptors, kinases, and synthetic molecular logic gates. While traditional directed evolution approaches excel at optimizing steady-state protein functionalities, they require substantial adaptation to treat transitions in states of proteins. This chapter describes how the emerging regulatory logic of autonomous biological oscillators can be used to impose time-varying selection pressure for evolving switchable proteins. We first review the challenges of evolving switchable proteins and examine past strategies, including fluorescence-activated cell sorting (FACS) screens, alternating chemical selection and counterselection methods, and phage display techniques. We then compare these with "optovolution," an approach recently developed by our lab, which couples a protein's activity to host cell cycle progression and leverages programmable light inputs to drive continuous evolution of proteins. To this end, we explain the design principles underlying the use of an autonomous oscillator for evolution and how it promotes selection for both "on" and "off" states, as well as switching between them. In a direct comparison with a conventional directed evolution method, we elucidate how optovolution proves more reliable by avoiding evolutionary routes that hinder counterselection. In conclusion we discuss how optovolution opens new avenues for creating proteins with complex logic and dynamic control, complementing latest advances in computational protein design.
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5. Synthetic Cell-Based Approaches to Dissect Autonomous Cellular Oscillators: Recent Applications on the Cell Cycle.
PMID:日期:2026-01-01Cell-free extracts have proven to be a tremendous tool for studying cell cycle regulation. This chapter reviews recent advances that expand upon this platform by incorporating synthetic cell-like compartments to encapsulate functional cell cycle regulators. These synthetic approaches allow for a programmable reconstitution of self-sustained autonomous oscillators, including that of mitosis, exhibiting a diverse range of biochemical and physical properties. The high throughput and manipulability of this system provide a comprehensive tool to systematically analyze the single-cell dynamics of this autonomous cell-cycle oscillator across broader yet finer-grained parametric, temporal, and spatial scales. We highlight key applications from the past few years and demonstrate how extending the well-established extract-based research with synthetic systems opens new avenues for tackling unexplored questions regarding cell cycle oscillations and their emergent phenomena.
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6. Autonomous Oscillations of NF-κB.
PMID:日期:2026-01-01The nuclear factor κB (NF-κB) signaling pathway plays a crucial role in regulating immune responses, inflammation, and cell survival. After the discovery of NF-κB by Ranjan Sen and David Baltimore as a nuclear factor that binds near the κ light-chain gene in activated B-cells [1], extensive research has been conducted to uncover key biochemical mechanisms underlying the pathway and the downstream genes regulated by NF-κB. The core regulatory module of NF-κB/IκB (inhibitor of NF-κB)/IKK (IκB kinase) has also been a leading example of cell signaling pathways that can produce oscillatory activities, particularly in response to the pro-inflammatory cytokine tumor necrosis factor α (TNF-α). Despite this status, the existence and function of NF-κB oscillations was a contentious topic in the early years. In this chapter, we touch on the history, progression, and outlook of the quest to decipher the properties and biological function of NF-κB oscillations.
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7. An Autonomous Clock that Controls Genetic Information Flow and Cellular Proteostasis.
PMID:日期:2026-01-01The central dogma of molecular biology, which delineates the flow of genetic information from DNA to RNA to proteins, is fundamental to cellular identity and functionality. This process involves intricate steps of transcription, mRNA processing, translation, and protein processing, each of which has been studied extensively over the past decades. Despite significant advancements in our understanding of these individual steps, the mechanisms by which these processes are spatiotemporally coordinated within each cell remain elusive. Efficient and precise transfer of genetic information is critical; any disruptions or "traffic jams" along this pathway including cryptic transcription, aberrant mRNA splicing, ribosome stalling, and protein misfolding and aggregation can lead to severe consequences such as aging and various diseases. Recently, our group has unveiled a cell-autonomous 12-h clock in mammalian cells that modulates the rhythmic activity of the entire genetic information flow. This discovery challenges the existing paradigm that genetic information flow is functioning at steady state but rather suggests that it oscillates with a robust 12-h cycle under normal physiological conditions, peaking at times of transition at dawn and dusk when metabolic stress is heightened. In this chapter, I will delve into the regulation, function, and evolutionary origins of the 12-h clock, and further elaborate how the 12-h clock can be utilized as a discovery tool to uncover hidden principles governing the spatiotemporal coordination of genetic information flow. Special attention will be given to the role of phase separation in facilitating this coordination, highlighting its significance in ensuring the seamless flow of genetic information across various cellular compartments and organelles.
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8. Autonomous Redox Rhythms and Their Coupling to the Circadian Clock.
PMID:日期:2026-01-01Circadian rhythms have traditionally been considered to be outputs of genetic transcription-translation feedback loop (TTFL) oscillators. However, the discovery of circadian peroxiredoxin oxidation rhythms in anucleate red blood cells suggests that cells also possess an autonomous circadian redox rhythm. The conservation of this rhythm across all lineages of life, as well as in TTFL-defective backgrounds in multiple organisms, further indicates its importance. Rather than functioning as an isolated system, the redox rhythm is bidirectionally coupled to the genetic circadian clock. Perturbation of one oscillation may alter amplitude or shift period of the other, depending on biological context. In animals, the interplay between the redox rhythm and the genetic clock is complex, and the biological significance of the redox oscillation has yet to be established. In plants, immune-related redox perturbation by salicylic acid can reinforce the genetic clock, while the redox rhythm itself gates immune-induced programmed cell death toward morning as a circadian output. We propose that the redox rhythm reflects an intrinsic metabolic cycling in which cells alternate between the high-metabolic states that generate energy, reducing power, and reactive oxygen species (ROS), and the lower-metabolic states that favor detoxification, repair, and restoration of redox homeostasis. In this model, the redox rhythm is driven by the feedback between glycolysis, pentose phosphate pathway, ROS production via electron transport chains, antioxidant capacity, and cellular repair, and becomes entrained to the external cues via diurnal energy generation such as photosynthesis in green lineages.
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9. Repurposing Cell Cycle Oscillators for Multiciliated Cell Differentiation.
PMID:日期:2026-01-01Repurposing extant resources is at the basis of functional innovation in evolution. In cell biology, conserved regulatory circuits, with minor adjustments to a subset of components, can be recruited for functional novelty. A prominent example concerns multiciliated cells that generate cilia-based fluid flows, vital for organism development and homeostasis. During their post-mitotic differentiation, these cells repurpose the autonomous oscillators that normally govern the cell division cycle to drive massive production of cilia-nucleating organelles, named centrioles, in the cytoplasm without committing to cell division. In this chapter, we review recent advances in understanding how a repurposed cell cycle machinery regulates these cytoplasmic dynamics essential for motile ciliogenesis.
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10. The Centriole Biogenesis Cycle: Temporal Mechanisms, Autonomy and Coupling to the Cell Cycle and Other Cellular Clocks.
PMID:日期:2026-01-01Centrioles are cytoskeletal organelles whose duplication must occur with high temporal precision to ensure faithful cell division, ciliogenesis and tissue organization. Traditionally, the centriole biogenesis cycle has been viewed as a surrogate to the nuclear division cycle, particularly to oscillations in cyclin-dependent kinase (CDK) activity. In this chapter, we revisit classic experimental observations demonstrating that centriole duplication can proceed independently of DNA replication, transcription and CDK oscillations, thereby revealing an intrinsic autonomy in this cycle. We then discuss emerging molecular evidence identifying oscillatory dynamics of Polo-like kinase 4 (Plk4) as the core autonomous clock mechanism that initiates and times centriole biogenesis. Building on this framework, we examine mechanisms that entrain or couple the centriole cycle to the mitotic cycle, ciliogenesis and circadian rhythms, highlighting shared regulatory modules such as ubiquitin ligases and phosphatases. We explore the physiological importance of maintaining proper coupling between these cycles through examples of cancer pathologies and developmental defects that arise when centriole timing becomes uncoupled. Finally, we outline outstanding questions regarding the temporal machinery that governs the centriole biogenesis cycle, its evolution, and emerging therapeutic opportunities, positioning this cycle as a model system for understanding how autonomous cellular clocks integrate into broader temporal control programmes in biology.