JOURNAL OF EXPERIMENTAL BIOLOGY实验生物学杂志
JOURNAL OF EXPERIMENTAL BIOLOGY(英文缩写 J EXP BIOL),ISSN 0022-0949,eISSN 1477-9145,中文译名:实验生物学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.308 | Q2 |
| 2022 | 2.800 | Q2 |
| 2023 | 2.800 | Q2 |
| 2024 | 2.600 | Q1 |
| 2025 | 3.000 | Q1 |
JOURNAL OF EXPERIMENTAL BIOLOGY 最新收录文献
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1. Phenanthrene disrupts calcium transients and inhibits INCX in zebrafish (Danio rerio) ventricular myocytes.
PMID:日期:2026-09-24Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) that is derived from fossil fuels and is present in most PAH mixtures found in water, air and soil. It has been shown to affect cardiac function of vertebrates, including fish. We investigated the effects of phenanthrene on intracellular calcium transient ([Ca2+]i) and the Na+/Ca2+ exchanger (NCX) current (INCX) in zebrafish (Danio rerio) ventricular cardiomyocytes using Fluo-4 AM imaging and whole-cell patch-clamp, respectively. Phenanthrene exposure (25 µM) reduced the amplitude and slowed the rate of decay of Ca²⁺ transients. Phenanthrene also inhibited INCX with equal efficacy in forward- and reverse-mode. We conclude that phenanthrene disrupts Ca²⁺ handling in zebrafish ventricular myocytes, which could impact cardiac contractility.
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2. Heart Rate Supports Accelerometry as a Reliable Predictor of Energy Expenditure in Bats.
PMID:日期:2026-09-24Measuring the energetic cost of behavior is essential for understanding decision-making in wild animals. This is particularly true for bats where life near their energetic ceiling has resulted in a diversity of behaviors to compensate for social and environmental unpredictability. While GPS and tri-axial accelerometry has improved movement-based estimates of energy expenditure in the field, complex flight kinematics in bats may violate key assumptions accelerometry-based estimates rely on. To test whether energy expenditure is predicted by acceleration-based movement in bats, we deployed heart rate loggers alongside GPS with tri-axial accelerometers on free-ranging greater spear-nosed bats (Phyllostomus hastatus) in Panamá. We hypothesized that heart rate would positively reflect increases in Vectorial Dynamic Body Acceleration (VeDBA), a commonly used proxy for energy expenditure, and that including information on behavior would enhance this relationship. We also hypothesized that heart rate and VeDBA would reflect theoretical speed-power curves of flight. We found VeDBA had a strong positive relationship with heart rate, and model accuracy improved when behavioral states of flying and not flying were defined. Additionally, incorporating behavioral duration and sequence number improved predictions, showing that temporal context and activity patterns further refine estimates of energy expenditure. The relationship between VeDBA and airspeed resembled the mechanical power curve previously estimated for P. hastatus, supporting the relevance of aerodynamic theory in interpreting bat energetics. Overall, acceleration-based estimates reliably predict energy expenditure in free-ranging P. hastatus and incorporating behavior improves model accuracy and ecological relevance, providing a powerful tool for understanding energy allocation in wild bats.
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3. Older adults do not have a higher metabolic cost than younger adults in outdoor overground walking.
PMID:日期:2026-09-21The metabolic cost of walking is widely used to evaluate human performance and effectiveness of clinical interventions. Decades of laboratory research, largely based on treadmill experiments, have established a canonical relationship between walking speed and metabolic cost, and suggested that ageing shifts this relationship upward, implying reduced efficiency in older adults. However, this relationship has not been well tested during overground walking across matched speeds. We compared healthy younger (n=16; 26±2yr) and older (n=11; 74±3yr) adults across eight outdoor overground walking trials at different speeds: preferred walking speed (PWS), three fixed speeds (0.8, 1.2, 1.6 m·s⁻¹), and four speeds at ±5% and ±10% of PWS. Contrary to our hypothesis, older adults did not show higher gross or net metabolic cost of walking (GCOW and NCOW) than younger adults at any speed; rather, both trended consistently lower in older adults, reaching significance for GCOW at 0.8 m·s⁻¹ only. Comparisons of resting metabolic rate and respiratory exchange ratio to prior reference groups did not indicate that our older cohort was unusually fit. Independent of age, GCOW was significantly higher at 0.8 m·s⁻¹ than at the remaining speeds (1.2-1.6 m·s⁻¹), confirming that walking at slower speeds increases GCOW. These findings challenge the view that ageing intrinsically increases the energetic cost of walking, suggesting instead that previously reported upward shifts in cost may reflect treadmill-specific constraints or speed effects. Future work is needed to explore direct comparisons of outdoor, overground walking with treadmill walking at fixed speeds in both younger and older adults.
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4. Bees exhibit behavioural flexibility to learned visual cues to prevent negative consequences during navigation in clutter.
PMID:日期:2026-09-21Bumblebees navigate complex environments where collisions with obstacles can impair flight performance. While bees possess innate collision avoidance reflexes, they may benefit from learning to identify and avoid high-risk collision areas using environmental cues. However, the temporal dynamics of how bees form associations between visual cues and collision experiences remain unclear. We investigated whether bumblebees associate visual cues perceived before or after collision events with a collision avoiding movement direction. Individual foragers were trained to navigate through a flight tunnel containing a transparent barrier and an LED panel that switched colours immediately after the bee's first collision. Following training, we tested bees' responses to each colour cue without the barrier. Bees demonstrated significant preferences for avoiding the previously blocked side when presented with either the colour shown before the first collision (81% avoidance-consistent choice) or the colour shown after the first collision (71% avoidance-consistent choice), while showing no preference when no colour cue was presented. These results are consistent with bees forming associations with visual cues encountered in different time windows relative to negative experiences. However, an equally plausible interpretation is that the bees learned primarily the association with the visual cue they saw before the first collision and then translated this learned response to the related, but distinguishable, cue they saw after the first collision. Both interpretations demonstrate considerable flexibility with which bees form and deploy memories to aid navigation in cluttered environments.
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5. A miRNA-mediated gene regulatory network supports seasonal plasticity in the temperate coral Astrangia poculata.
PMID:日期:2026-09-21Phenotypic plasticity is critical for sessile marine invertebrates facing intensifying climate change. MicroRNAs (miRNAs)-small non-coding RNAs that regulate gene expression-are compelling candidates for mediating such plasticity, but their role in coral biology remains poorly characterized. We used the temperate coral Astrangia poculata, which endures annual temperature ranges exceeding 25°C, to investigate miRNA-mediated plasticity across seasonal and thermal contexts. Adult aposymbiotic colonies were exposed to ambient seasonal temperatures (∼5-22°C) and a chronic +3°C warming treatment from February to August 2021. Monthly physiological sampling showed that seasonal temperature variation drove significant shifts in photosynthesis, respiration, and soluble protein, whereas the +3°C treatment had minimal physiological effect. RNA-seq across three time points (February, June, and August) revealed that seasonal temperature variation also drove the strongest molecular response. Transcriptional functional enrichment shifted from winter protein homeostasis and cellular integrity to summer immunity, metabolism, and reproduction. We also identified 51 A. poculata miRNAs (46 novel), providing the first characterization of the miRNA repertoire in this species. Target prediction and co-expression analyses revealed that while mRNA-miRNA networks appear to maintain a stable infrastructure across seasons and treatments, specific interactions may be rewired to drive seasonal biology. This dynamic regulation is associated with putative suppression of energy-intensive cell division and morphogenesis during winter quiescence, while shifting to regulate tissue remodeling and reproduction genes during the summer. These results establish miRNAs as seasonal gene regulators in a temperate coral and suggest that the molecular infrastructure underlying its plasticity may also confer resilience to moderate thermal stress.
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6. Pursuit dynamics among australian marsupials and dogs: Comparative locomotor performance and escape probability.
PMID:日期:2026-09-17Predation can strongly influence population outcomes, and locomotor performance may be especially important during pursuit-evasion interactions. We hypothesised that differences in performance-based escape capacity could contribute to the abundance and distribution of prey populations. We compared the locomotor performance of northern brown bandicoots (Isoodon macrourus), which persist across mainland Australia, and northern quolls (Dasyurus hallucatus), which have declined on the mainland, with that of domestic dogs (Canis familiaris; multiple breeds, 3.4-44 kg) as an approximation of canid performance. Velocity, acceleration and changes in heading were extracted from top-down video footage and used to estimate relative escape performance. Dogs achieved the highest mean and maximum speeds, whereas quolls showed the greatest turning capacity. Bandicoots were the slowest, least agile and most predictable of the three groups. The ability of quolls to outmanoeuvre a pursuing predator may help explain their association with structurally complex habitats, where agility should provide a greater advantage. By contrast, the persistence and abundance of northern brown bandicoots are likely supported by other ecological traits because their locomotor capacity for avoiding pursuit predators appears limited. These findings show that locomotor performance alone cannot explain prey population outcomes, but may interact with habitat complexity to favour different escape strategies.
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7. Characterizing choice behavior in Drosophila olfactory classical conditioning.
PMID:日期:2026-09-17Drosophila melanogaster is a powerful model organism to understand the neural and molecular mechanisms underlying learning and memory. However, memory is usually studied by assessing choice behavior at only one point in time in Drosophila, which limits our understanding of how flies respond to a learned cue. We developed a simple open-field-style assay that records the continuous behavioral dynamics of flies as they form preferences between two competing olfactory stimuli. We trained flies to associate odors with either a reward (sucrose) or punishment (electric shock) and, then placed them in a circular arena where they encountered a choice between a paired or unpaired odor. Within 30 seconds, flies approached the sugar-associated odor or avoided the shock-associated odor. Their collective behavior dynamics suggest the flies became more decisive over time; they traveled more on the preferred side, stayed closer to the source of the preferred cue, made more stops there, and consistently turned away from the alternative cue. We confirmed these results by showing that a learning-deficient mutant strain displayed no choice behavior. This assay provides enhanced resolution for understanding decision-making as a continuous, dynamic process in Drosophila.
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8. Vocal plasticity at different time spans during exposures to biotic and abiotic natural sounds in an austral temperate forest frog.
PMID:日期:2026-09-17Several instances of short-term plasticity of reproductive behaviors are known to modulate interactions among competing individuals. Habituation and sensitization processes result in decrements and increments, respectively, of agonistic behaviors in breeding areas. The occurrence of such phenomena in the long term have been reported to a quite restricted extent. Frogs communicating acoustically experience auditory activation along days of exposure to conspecific signals and thus we looked for vocal activation over such time span in male frogs Batrachyla taeniata. A total of 72 frogs were exposed during 8 or 10 days to either 5 hours of either broadcast of natural chorus sound, rain sound or left in silence, and subjected to stimulation with synthetic conspecific calls before and after such treatments. Results showed no significant variation of vocal activity between pre- and post- exposure days, indicating that no long-term plasticity phenomena occurred. However within each day of exposure an initial increment followed by a decrement in call rate were observed, pointing to processes of short-term sensitization and habituation or fatigue, respectively, but potential effects of photoperiod and energy depletion also need to be assessed. The lack of long-term sensitization of vocal activity contrasts with the auditory system activation for such time span reported for other anurans. The mismatch between both phenomena could relate to constraints imposed by the high energy expenditure that characterizes anuran vocal activity.
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9. Surface texture guides egg-laying decisions in Aedes aegypti mosquitoes.
PMID:日期:2026-09-15Mosquitoes undergo development as aquatic larvae and pupae before emerging as terrestrial adults. Accordingly, blood-fed and mated female mosquitoes must select an appropriate egg-laying site to maximize the fitness of their offspring. Female yellow fever mosquitoes (Aedes aegypti) lay their eggs above the waterline of small containers or natural bodies of water, where they can remain dormant for many months until they are submerged and hatch. Here, we show that female mosquitoes use surface texture as a powerful cue to guide egg-laying decisions, selecting rougher textures over smooth when choosing among containers and when selecting specific sites within a given substrate. In addition, we identify an interaction between substrate texture and water salinity with respect to egg-laying decisions, demonstrating that female mosquitoes integrate competing cues to determine the ultimate suitability of an egg-laying site. Finally, we explore the dynamics of local egg-laying search behaviour, demonstrating that texture modulates traversal speed while mosquitoes search for appropriate egg-laying sites.
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10. Flight energetics of barn swallows across speeds: high aerodynamic efficiency, but low energy conversion efficiency.
PMID:日期:2026-09-15Bird flight conversion efficiency (η) determines the amount of metabolic power (Pmet) transformed into mechanical power (Pmech) to remain aloft, and it is central to understanding animal flight energetics, yet it remains difficult to quantify. Using 13C-labelled sodium bicarbonate (NaBi) and particle image velocimetry (PIV), we measured Pmet and Pmech, respectively, in un-instrumented barn swallows (Hirundo rustica) flying across 7.8 to 13.6 m s-1 in a wind tunnel. Both Pmet and Pmech showed the expected U-shaped relationship with airspeed. Whole-animal η showed a shallow peak at 7% at 11 m s-1. Partial efficiency (ηp), estimated from horizontal and 4 deg climbing flight, was 27% (95% confidence interval CI: -2 to 56.1). Compared with thrush nightingales previously studied under identical conditions, barn swallows exhibited higher weight-specific flight Pmet but lower lift-specific Pmech, reflecting a higher basal metabolic rate but greater aerodynamic efficiency in barn swallows. Barn swallows showed lower peak and flatter η curve across speeds compared with that of the nightingales, suggesting a trade-off between flight energetics and flight speed: higher efficiency peak over a narrow speed range (thrush nightingales) versus lower, more consistent efficiency across a broader speed range (barn swallows). Whereas nightingales fly mainly during migration, barn swallows spend much of the day airborne and use a wider range of flight speeds, potentially favouring versatility over a narrow η peak. Although η has been studied in few species, our results suggest that η is both species and speed dependent, probably shaped by species-specific ecological demands.