ANALYTICAL LETTERS分析快报
ANALYTICAL LETTERS(英文缩写 ANAL LETT),ISSN 0003-2719,eISSN 1532-236X,中文译名:分析快报 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.267 | Q3 |
| 2022 | 2.000 | Q3 |
| 2023 | 1.600 | Q3 |
| 2024 | 1.800 | Q3 |
| 2025 | 1.800 | Q3 |
ANALYTICAL LETTERS 最新收录文献
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1. Quantitation of Arsenic in biological matrices: Use of Sector Field Inductively Coupled Plasma-Mass Spectrometry for Improved Accuracy and Specificity.
PMID:日期:2025-04-29Arsenic (As) and As-containing compounds have a wide array of applications in medicine, industry, and agriculture and has been associated with adverse health outcomes. In support of studies investigating the toxicity and toxicokinetic behavior of arsenic compounds, we developed and validated a high-resolution sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) method to quantitate total arsenic in Sprague-Dawley (SD) rat plasma and heart. Primary matrix standard curves prepared in male SD rat plasma were linear (r ≥ 0.999) over the range of 5 to 1250 ng As/mL plasma with accuracy determined as relative error (RE) was ≤ ±20% at the lower limit of quantitation (LLOQ) of 5 ng/mL and ≤ ±15% for all other concentrations. The method limit of detection (LOD) was 0.728 ng/mL plasma. Mean extraction recovery was 96.7%, with a relative standard deviation (RSD) of 4.0%. The method demonstrated acceptable inter- (≤ ±7.3% RE and ≤ 8.0% RSD) and intra-day (≤ ±14.9% RE and ≤ 6.8% RSD) accuracy and precision. Method selectivity was ≤ 20% of the response for the LLOQ. Method performance was also evaluated in SD rat heart by preparing QC samples in heart homogenate and quantifying using plasma calibration curve. The mean RE value was -3.8% and the RSD value was 3.4% demonstrating that the method is suitable for quantitation of arsenic in rat heart.
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2. Development of a Rapid Fluorescence Probe for the Determination of Aqueous Hypochlorite.
2. 快速荧光探针用于测定水溶液中次氯酸盐的研究PMID:日期:2024-09-17Chlorine interacts with the aqueous environment, including the mucosa and alveolar fluid in the lungs, causes the immediate transformation of chlorine to hypochlorous acid (HClO) and hydrochloric acid (HCl) which induces cellular damage and may produce systemic toxicity. There are multiple techniques to monitor hypochlorite (HClO/ClO) from various matrices, however, they each have significant disadvantages, including limited sensitivity, complexity, and slow response. In this study, we developed a water-soluble fluorescein-based probe, called fluorescein thioacid (FSH), for the rapid and sensitive detection of HClO/ClO in aqueous solutions via oxidation of FSH to produce fluorescein. FSH was synthesized via a simple, one-step procedure and exhibited excellent selectivity for ClO, maintaining 82% signal stability for 24 h. FSH performed well over a wide pH range (4 to 10; optimum pH of 4), produced a linear range from 1 to 100 μM, and had a detection limit of 100 nM. The probe was able to accurately quantify HClO/ClO concentrations in tap water (13.8 μM) and swimming pool water (56.9 μM) samples. Overall, FSH is simple to synthesize, highly selective, highly sensitive, has excellent water solubility, and produces a fast response for monitoring of aqueous ClO levels.
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3. High-Throughput Monitoring of Pathogenic Fungal Growth Using Whole Slide Imaging for Rapid Antifungal Susceptibility Assessment.
PMID:日期:2023-12-29Invasive fungal infections are a major health threat with high morbidity and mortality, highlighting the urgent need for rapid diagnostic tools to detect antifungal resistance. Traditional culture-based antifungal susceptibility testing (AFST) methods often fall short due to their lengthy process. In our previous research, we developed a whole-slide imaging (WSI) technique for the high-throughput assessment of bacterial antibiotic resistance. Building on this foundation, this study expands the application of WSI by adapting it for rapid AFST through high-throughput monitoring of the growth of hundreds of individual fungi. Due to the distinct "budding" growth patterns of fungi, we developed a unique approach that utilizes specific cell number change to determine fungi replication, instead of cell area change used for bacteria in our previous study, to accurately determine the growth rates of individual fungal cells. This method not only accelerates the determination of antifungal resistance by directly observing individual fungal cell growth, but also yields accurate results. Employing as a representative model organism, reliable minimum inhibitory concentration (MIC) of fluconazole inhibiting 100% cells of (denoted as MIC) was obtained within 3h using the developed method, while the modified broth dilution method required 72h for the similar reliable result. In addition, our approach was effectively utilized to test blood culture samples directly, eliminating the need to separate the fungi from whole blood samples spiked with . These features indicate the developed method holds great potential serving as a general tool in rapid antifungal susceptibility testing and MIC determination.
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4. Do Storage Conditions Affect Collected Cookstove Emission Samples? Implications for Field Studies.
PMID:日期:2022-12-20Cookstove emissions are a significant source of indoor air pollution in developing countries and rural communities world-wide. Considering that many research sites for evaluating cookstove emissions and interventions are remote and require potentially lengthy periods of particulate matter (PM) filter sample storage in sub-optimal conditions (e.g., lack of cold storage), an important question is whether samples collected in the field are stable over time. To investigate this, red oak was burned in a natural-draft stove, and fine PM (PM) was collected on polytetrafluoroethylene filters. Filters were stored at either ambient temperature or more optimal conditions (-20°C or -80°C) for up to 3 months and extracted. The effects of storage temperature and length on stability were evaluated for measurements of extractable organic matter (EOM), PM, and polycyclic aromatic compound (PAC) levels in the filter extracts. A parallel, controlled laboratory condition was also evaluated to further explore sources of variability. In general, PM and EOM in both simulated field and laboratory samples were similar regardless of the storage condition or duration. The extracts were also analyzed by gas chromatography to quantify 22 PACs and determine similarities and/or differences between the conditions. PAC levels were a more sensitive stability measure in differentiating between storage conditions. The findings suggest that measurements are relatively consistent across storage duration/temperatures for filter samples with relatively low EOM levels. This study aims to inform protocols and filter storage procedures for exposure and intervention research conducted in low- and middle-income countries where studies may be budget- and infrastructure-limited.
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5. Highly selective fluorescent probe with an ideal pH profile for the rapid and unambiguous determination of subcellular labile iron (III) pools in human cells.
5. 高选择性荧光探针，具有理想的pH响应特性，可用于快速、准确地测定人体细胞中亚细胞不稳定铁(III)池PMID:日期:2022-03-31A convenient tool for detecting iron ions in subcellular structures is desired for better understanding its roles in biological systems. In this work, a new Fe sensor, 2-(2-((1-(6-acetylpyridin-2-yl)ethylidene)amino)ethyl)-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one (RhPK), which operates across the entire cellular pH range and is capable of unambiguously detecting Fe ion in live human cells at subcellular resolution, is reported. The sensor exhibits high selectivity and sensitivity toward Fe with a rapid fluorescence response and a 12-fold increase in intensity upon the addition of 1 equivalent Fe at pH 7.3. RhPK forms a 1:1 complex with Fe with an apparent binding constant 1.54 × 10 M and a detection limit of 50 nM. The sensor is stable between pH 4.2 and 9.0 and operates across the whole cellular pH range. Cell imaging demonstrates the ability of the sensor to unambiguously detect basal level Fe as well as its dynamic changes in real-time in live cells at subcellular resolution, with one labile Fe pool identified in mitochondria in human primary fibroblast (ws1) cells for the first time and two Fe pools confirmed in mitochondria and endo/lysosomes in human SH-SY5Y neuroblastoma cells, suggesting different cell types have distinctive Fe storage in subcellular compartments. The RhPK probe is powerful for rapid and sensitive bioimaging of Fe at subcellular level, enabling the unambiguous detection of labile Fe pools at the entire cellular pH range, which is of great significance to understand the biological chemistry of Fe and its roles in physiological processes and diseases.
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6. Quantitation of Phenolic Benzotriazole Class Compounds in Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
6. 血浆中酚类苯并三唑类化合物的液相色谱-串联质谱（LC-MS/MS）定量分析PMID:日期:2022-03-02Phenolic benzotriazoles are used as UV stabilizers in consumer products and have been detected in the environment suggesting potential human exposure. Phenolic benzotriazoles were nominated to the Division of National Toxicology Program for testing based on their potential widespread human exposure and lack of adequate toxicity data. Nine chemicals were selected for toxicological evaluation, representing unsubstituted (2-(2H-benzotriazole-2-yl)phenol, (P-BZT)), monosubstituted (drometrizole; 2-(2H-benzotriazol-2-yl)-4-tert-butylphenol (tBu-BZT); octrizole), disubstituted (2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (diMeEtPh-BZT), 2-(2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylpropyl)phenol (ditPe-BZT); 3-(2H-benzotriazol-2-yl)-5-(1,1-dimethylethyl)-4-hydroxybenzenepropanoic acid, octylester (tBuPrOcEst-BZT) and halogenated trisubstituted (bumetrizole; 2-(5-chloro-2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylethyl)phenol (ditBuCl-BZT)) compounds. Different extraction methods were utilized and methods were developed to analyze phenolic benzotriazoles by quantitating free (unconjugated parent) and total (free and conjugated parent) analyte levels in plasma of rats to aid in interpretation of toxicity data, understanding of absorption, distribution, metabolism, and excretion differences. The calibration standard range was 1-500 ng/mL for free analytes and 1-1000 ng/mL for total analytes. The methods were linear (r ≥ 0.99). The accuracy was determined as relative error (RE) and ranged from -18.2 to +17.8, and precision was determined as relative standard deviation (RSD) and ranged from 0.0 to 20.1% for both free and total plasma calibration standards, respectively. The limit of quantitation was ≤ 5.0 and 10.0 ng/mL and limit of detection was ≤ 1.2 and 2.0 ng/mL, for free and total analytes, respectively. These data demonstrate that the methods are suitable for quantitation of free and total analytes in rat plasma.
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7. {"_":"Development and Validation of an Analytical Method to Quantitate Hydroxycitric Acid, the Key Constituent in Extract, in Rodent Plasma and Fetus.","i":["Garcinia cambogia"]}
PMID:日期:2021-12-11extract (GCE) is a popular botanical supplement used in weight loss products. Hydroxycitric acid (HCA) is the principal component in GCE. Due to lack of adequate toxicity data to assess the safe use of GCE, the National Toxicology Program is testing GCE in Hsd:Sprague Dawley® SD® rats following perinatal exposure and in adult B6C3F1/N mice. We report a validated method utilizing sample clean up with ultrafiltration followed by liquid chromatography-tandem mass spectrometry analysis to quantify HCA in rat plasma over the concentration range of 20 to 800 ng/mL. The method was linear (r ≥ 0.99) with the limits of quantitation (LOQ) and detection (LOD) of 20.0 and 3.9 ng/mL plasma, respectively. The accuracy (determined as relative error, RE) and precision (determined as relative standard deviation, RSD) using Quality Control standards analyzed over multiple days were ≤ ± 7.5% and ≤ 9.5%, respectively. The method can be applied to quantify HCA in study matrices (RE ≤ ± 23.0%; RSD ≤ 6.0) except gestational day (GD)18 fetus. The method was partially validated in GD18 fetal homogenate over the concentration range 60-3000 ng/g (r ≥ 0.99, RE ≤ ± 11.9%, and RSD ≤ 5.5%; LOQ 60.0 ng/g; LOD 7.77 ng/g). The standards as high as 20,000 ng/mL (plasma) and 502,000 ng/g (fetus) can successfully be quantified after diluting into the validated range (RE ≤ ± 2.6%; RSD ≤ 5.2%). These data demonstrate that the method is suitable to quantify HCA in rodent matrices and can be adapted to other biological matrices.
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8. Validation of Analytical Method for Determination of Thallium in Rodent Plasma and Tissues by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
8. 采用电感耦合等离子体质谱法（ICP-MS）测定啮齿类动物血浆和组织中铊含量的分析方法验证PMID:日期:2021-12-08Thallium (Tl) can be released as a byproduct of smelting, mining, and other industries, causing human exposure. There are knowledge gaps on the toxicity of thallium compounds, so the National Toxicology Program is investigating the toxicity of thallium (I) sulfate in rodents. We developed and validated a method to quantitate Tl in rodent plasma and secondary matrices. Primary matrix standards and validation samples were digested with nitric acid and analyzed for Tl by inductively-coupled plasma - mass spectrometry (ICP-MS). Method performance was validated for linearity, accuracy, precision, and other criteria. Calibration was linear from 1.25 to 500 ng Tl/mL plasma; accuracy (RE) was -5.9 to 2.6% for all calibration standards. The lower limit of quantitation (LLOQ) was 1.25 ng Tl/mL plasma, and the limit of detection was 0.0370 ng Tl/mL plasma. Intra- and interday RE and precision (RSD) were -5.6 to -1.7% and ≤0.8% (intraday) and -4.8 to -1.3% and ≤4.3% (interday), respectively, at three sample concentration levels. Standards up to 10.0 × 10 ng/mL could be analyzed by dilution with digested blank matrix, with -6.4% RE and 5.4% RSD. Method was also evaluated in post-natal day 4 (PND4) Hsd:Sprague Dawley SD (HSD) dam and pup plasma, gestation day 18 (GD 18) HSD rat fetal homogenate, HSD rat urine, female HSD rat brain homogenate, female B6C3F1 mouse plasma. Background Tl was detected in control fetal and brain homogenates and urine at < 30% of LLOQ response. Results demonstrate that the method is suitable for determination of Tl in rodent matrices for toxicology studies.
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9. Development and validation of an ultraperformance liquid chromatography-tandem mass spectrometry method for quantitation of total 3,3',5-triiodo-L-thyronine and 3,3',5,5'-tetraiodo-L-thyronine in rodent serum.
9. 超高效液相色谱-串联质谱法测定啮齿动物血清中总3,3',5-三碘-L-甲状腺原氨酸和3,3',5,5'-四碘-L-甲状腺原氨酸的建立与验证PMID:日期:2021-08-27Many environmental chemicals are known to disrupt thyroid function. Measurement of thyroid hormones in animal studies provides useful information to understand the effects of environmental chemicals on thyroid hormone metabolism. We report an efficient method, utilizing a protein precipitation followed by ultraperformance liquid chromatography-tandem mass spectrometry analysis, to quantitate total 3,3',5-triiodo-L-thyronine (triiodothyronine, T3) and total 3,3',5,5'-tetraiodo-L-thyronine (thyroxine, T4) in rodent serum. The use of synthetic serum for calibration standards eliminated the interferences from endogenous total T3 and T4 and allowed the experimental lower limits of quantitation (LOQ) to be set at the required concentration (T3, 20 ng/dL; T4, 0.5 μg/dL) to allow quantitation of endogenous concentrations. The method was linear (r>0.99; range 20.0-600 ng/dL T3, 0.500-15 μg/dL T4) with good assay recoveries (90.4-107%) for both analytes. Intra- and inter-day accuracy, estimated as percent relative error, were ≤ ±7.6% and intra- and inter-day precision, estimated as the relative standard deviation, were ≤ 5.3% for both analytes. The method may easily be adapted to a well-plate format thereby further improving the efficiency. Total T3 and T4 concentrations were stable in male and female rat and mouse serum when stored in the freezer (~ -70 °C) for up to 62 d with determined values within 92.8-111% of day 0 for both analytes. The method can be extended to quantitate total T3 and T4 concentrations in humans or other species with minimal optimization.
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10. Quantitation of Total Vanadium in Rodent Plasma and Urine by Inductively Coupled Plasma - Mass Spectrometry (ICP-MS).
10. 采用电感耦合等离子体质谱法（ICP-MS）测定啮齿动物血浆和尿液中总钒的含量PMID:日期:2021-03-01Human exposure to vanadium (V) is anticipated because it is a drinking water contaminant. Due to limited data on soluble V salts, the National Toxicology Program is investigating the toxicity in rodents following drinking water exposure. Measurement of internal V dose allows for interpretation of toxicology data. The objective of this study was to develop and validate an inductively coupled plasma-mass spectrometric method to quantitate total V in rat plasma. The method was linear (r ≥ 0.99) from 5.00 - 1,000 ng V/mL. Intra- and inter-day relative error (% RE) and relative standard deviation (% RSD) of spiked plasma samples were 8.5% - 15.6% RE and ≤ 1.8% RSD and 7.3% - 11.7% RE and ≤ 3.1% RSD, respectively. The limit of detection was 0.268 ng V/mL plasma and absolute percent recovery was 113%. Standards up to 7,500 ng V/mL plasma were diluted into the validated range (5.6% RE, 0.9% RSD). V in extracted plasma samples over 15 days at ambient and refrigerated conditions was from 97.7 - 126% of day 0. Determined plasma V concentrations after three freeze-thaw cycles and after frozen storage for up to 63 days ranged from 100 - 106% and 100 - 122% of day 0, respectively. The method was extended to rat urine (accuracy and precision -2.0 - 0.3% RE and <0.6% RSD, respectively for same linear range). These data demonstrate that the method is suitable to quantitate V in rat plasma and urine.