ACTA BIOCHIMICA POLONICA波兰生物化学学报
ACTA BIOCHIMICA POLONICA(英文缩写 ACTA BIOCHIM POL),ISSN 0001-527X,eISSN 1734-154X,中文译名:波兰生物化学学报 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 2.349 | Q4 |
| 2022 | 1.700 | Q4 |
| 2023 | 1.400 | Q4 |
| 2024 | 1.700 | Q4 |
| 2025 | 2.200 | Q4 |
ACTA BIOCHIMICA POLONICA 最新收录文献
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1. Cyclotorsion as a structural and functional biomarker of ocular and neuroophthalmic diseases.
1. 旋转偏斜作为眼部和神经眼科疾病的结构与功能生物标志物PMID:日期:2026-09-18Cyclotorsion, defined as rotation of the eye around its anteroposterior axis, is an important but often underrecognized component of ocular motility. This review summarizes the physiological basis of cyclotorsion, methods of subjective and objective assessment, and the clinical relevance of torsional deviations in ocular and neuro-ophthalmic disorders. Particular emphasis is placed on objective measurement using the disc-foveal angle and its role in diagnosing and monitoring conditions such as trochlear nerve palsy, skew deviation, pulley disorders, inferior oblique overaction, thyroid eye disease, heavy eye syndrome, and sagging eye syndrome. Quantitative assessment of cyclotorsion may improve diagnostic accuracy, support surgical planning, and provide an objective marker of treatment outcome.
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2. Diagnostic value of endothelial-specific molecule-1 levels in patients with bone tumors.
PMID:日期:2026-09-10In suspected bone tumors where imaging and histopathological evaluation are fundamental for diagnosis, minimally invasive blood biomarkers are needed for earlier diagnosis, initial assessment, and risk stratification. The aim of this research was to evaluate the diagnostic value of Endothelial-Specific Molecule-1 (ESM-1) levels in determining the presence of bone tumors and distinguishing between benign and malignant tumors. This cross-sectional research, conducted at a research hospital, involved 60 participants diagnosed with bone tumors and 30 healthy participants. ESM-1 values were analyzed using Enzyme-Linked Immunosorbent Assay (ELISA). The data obtained were evaluated using binary logistic regression analysis and Receiver Operating Characteristic (ROC) curve analysis. ESM-1 values were determined to be higher in the bone tumors group than in the control group. ROC analysis showed an Area Under the Curve (AUC) of 0.895. Using a cut-off value of 138.94 ng/L for detecting bone tumors, the sensitivity and specificity were 78.3% and 80.0%, respectively. In addition, ESM-1, erythrocyte sedimentation rate, and C-reactive protein levels were found to be higher in the malignant subgroup than in the benign subgroup. In subgroup ROC analysis, ESM-1 perfectly discriminated against malignant tumors from both benign tumors (AUC = 1.000) and healthy controls (AUC = 1.000), with sensitivities and specificities of 100% at cut-off values of 204.54 ng/L and 195.00 ng/L, respectively. For differentiating benign tumors from healthy controls, the AUC was 0.789, with 70.0% sensitivity and 70.0% specificity at a cut-off value of 125.54 ng/L. ESM-1 levels may have potential diagnostic value in distinguishing between bone tumors and malignant tumors; however, these results need to be confirmed in larger samples.
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3. {"_":"Bioactivity screening of plant ethanol extracts against and field evaluation of an optimized botanical combination formulation.","i":["Plutella xylostella"]}
3. {"_":"植物乙醇提取物对小菜蛾的生物活性筛选及优化植物组合制剂的田间评价。","i":["小菜蛾"]}PMID:日期:2026-08-27is a major pest of cruciferous vegetables. The prolonged overuse of chemical insecticides has resulted in increased resistance in populations, highlighting the urgent need for environmentally friendly and effective alternative control strategies. In this study, ethanol extracts from 15 plant species collected from the Qinling Mountains of China were systematically evaluated for their insecticidal, antifeedant, and oviposition-repellent activities against Laboratory bioassays were conducted using leaf-dipping and leaf-disc methods. Among the tested plants, ethanol extracts of and exhibited the strongest biological activities. Specifically, extract achieved corrected mortality, antifeedant, and oviposition-repellent rates of 83.11%, 89.46%, and 78.57%, respectively, whereas extract produced corrected mortality, antifeedant, and oviposition-repellent rates of 73.53%, 87.45%, and 82.14%, respectively. When combined at a volume ratio of 8:2, the two extracts showed enhanced insecticidal activity, with a median lethal concentration (LC50) of 65.762 mg/mL and a co-toxicity coefficient consistent with an additive effect without antagonism. A stable emulsifiable concentrate (EC) formulation was subsequently developed through optimization of the solvent and surfactant system. Field trials demonstrated that the 10% botanical EC effectively suppressed populations, achieving a control efficacy of 58.35% at 7 days post-application, which was comparable to that of the commonly used insecticide emamectin benzoate. Collectively, these findings suggest that and have potential as botanical insecticide resources for the integrated management of and may contribute to more sustainable agricultural practices.
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4. Hematological and biochemical alterations in patients with suspected COVID-19 infection with negative and positive RT-PCR swabs.
4. 疑似COVID-19感染且RT-PCR拭子阴性和阳性患者的血液学和生化改变PMID:日期:2026-08-18Hematological and biochemical abnormalities have been reported in patients with confirmed cases of coronavirus disease 2019 (COVID-19). This study compared selected hematological and biochemical parameters in patients with confirmed COVID-19 (RT-PCR positive) and suspected COVID-19 (RT-PCR negative). A retrospective cross-sectional study was conducted among 106 patients admitted to the COVID-19 isolation centers at Ibn-Sina Hospital, Mukalla, Yemen, from 1 December 2021 to 30 October 2022. The study included 63 men and 43 women, with a mean age of 58.3 ± 17.5 years. Demographic, clinical, hematological, and biochemical data were extracted from medical records. The following were analyzed: complete blood count (CBC), random blood glucose (RBG), alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), C-reactive protein (CRP), and ferritin. Of the 106 patients, 58 (54.72%) had confirmed (RT-PCR positive) COVID-19, and 48 (45.3%) had suspected (RT-PCR negative) COVID-19. Compared with the RT-PCR-negative group, the RT-PCR-positive patients had significantly higher hemoglobin, hematocrit, red blood cell (RBC), and white blood cell (WBC) counts and were more likely to exhibit neutrophilia, lymphopenia, and eosinopenia. Serum LDH, GGT, CRP, and ferritin levels were also significantly higher in the RT-PCR-positive group. Using binary logistic regression analysis, LDH (OR: 1.008, 95% CI: 1.002-1.013, P = 0.004) and GGT (OR: 0.949, 95% CI: 0.903-0.997, P = 0.039) were independently associated with RT-PCR positivity. Among the investigated laboratory markers, LDH and GGT showed independent associations with RT-PCR positivity. Given the retrospective cross-sectional design, these findings should be interpreted as associations rather than evidence of predictive or causal relationships.
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5. Urinary tract infections in diabetes: clinical significance and management approaches.
5. 糖尿病中的尿路感染：临床意义与管理方法PMID:日期:2026-08-17Diabetes mellitus is a significant risk factor for urinary tract infections (UTIs), which occur more frequently, have a more severe course, and show a greater tendency to recur in affected individuals. Metabolic disturbances, particularly hyperglycemia and glucosuria, promote bacterial growth while simultaneously impairing innate and adaptive immune responses. In addition, diabetes-related complications, such as autonomic neuropathy, contribute to bladder dysfunction and urinary stasis, further facilitating bacterial colonization. Epidemiological evidence indicates a two- to four-fold increased risk of UTIs in patients with diabetes, along with a high prevalence of asymptomatic bacteriuria and recurrent infections. A key feature of this relationship is its bidirectional nature. UTIs can exacerbate glycemic dysregulation by triggering stress responses and increasing insulin resistance, leading to transient or sustained hyperglycemia. Uropathogenic plays a critical role in the pathogenesis of UTIs, with hyperglycemic conditions further enhancing bacterial adhesion, biofilm formation, and immune evasion. Effective management requires simultaneous control of infection and optimization of glycemic control, particularly in the context of rising antimicrobial resistance. Emerging therapeutic strategies include targeting bacterial adhesion, disrupting biofilms, and modulating host immune responses. Improved understanding of host-pathogen interactions may enable the development of more effective preventive and therapeutic approaches in this high-risk population.
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6. Gap junction protein connexin 36 proteostatic mechanisms in photoreceptor cells subjected to excitotoxic stress.
6. 受兴奋性毒性应激的感光细胞中缝隙连接蛋白连接蛋白36的抑蛋白机制PMID:日期:2026-08-14Connexin 36 (Cx36), a neuronal gap junction protein, is essential for retinal signal transmission but may also contribute to neurodegeneration by facilitating the spread of excitotoxic stress signals. This study aimed to examine the degradation pathway of Cx36 under stress conditions relevant to retinal disease. Using immortalized 661W murine photoreceptor cells transfected with GFP-tagged Cx36, we evaluated the effects of lysosomal inhibition (Bafilomycin A1) and excitotoxic stimulation (NMDA). Immunofluorescence revealed intracellular Cx36 accumulation following Bafilomycin and NMDA exposure, accompanied by increased expression of p62/SQSTM1 and PSMA1, suggesting impaired autophagic flux and a potential compensatory involvement of the proteasome. Elevated RAB11A levels suggested recruitment of endosomal recycling pathways. NMDA treatment was associated with increased ubiquitin accumulation, particularly in Cx36-overexpressing cells, indicating enhanced proteostatic stress under excitotoxic conditions. These findings highlight the stress-responsive behavior of Cx36 and suggest that connexin proteostasis may represent a potential target for future investigation in retinal neurodegeneration, including glaucoma and age-related macular degeneration. Immunofluorescence revealed intracellular accumulation of p62 and PSMA1 after Bafilomycin exposure with signal intensities increasing approximately 1.5-2-fold versus controls (n = 3, p < 0.05). These data are consistent with disrupted autophagic flux and suggest proteasome involvement, but do not constitute functional proof.
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7. Optimization of a TEEDA-based initiation system for SDS-PAGE.
7. 基于TEEDA的SDS-PAGE引发系统的优化PMID:日期:2026-08-12Polyacrylamide gel preparation for SDS-PAGE is conventionally initiated by the ammonium persulfate (APS)-TEMED system. Although effective, the relatively high volatility of TEMED may increase operator exposure during routine laboratory use. In this study, N,N,N',N-tetraethylethylenediamine (TEEDA) was systematically evaluated as an alternative accelerator for SDS-PAGE gel preparation. TEEDA alone produced polymerization and protein separation performance comparable to those of the conventional TEMED-APS system, although higher concentrations (8.3 mM in 10% separating gels and 17.4 mM in 5% stacking gels) were required than those of TEMED (4.0 mM and 6.7 mM, respectively). When TEEDA concentrations comparable to those of TEMED were used, supplementation with 0.1% SBS in separating gels and 0.01% SBS in stacking gels restored polymerization and protein separation performance to levels comparable with those of the conventional TEMED-APS system. These findings provide an optimized TEEDA-based formulation for routine SDS-PAGE.
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8. Correction: Value evaluation of serum (sdLDLc*HCYc)/HDLc ratio in the stability of intracranial arterial plaques in patients with acute cerebral infarction.
8. 更正：血清(sdLDLc*HCYc)/HDLc比值对急性脑梗死患者颅内动脉斑块稳定性的价值评估PMID:日期:2026-07-29[This corrects the article DOI: 10.18388/abp.2020_6817.].
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9. The yields of nucleic acids and proteins extracted from various murine tissue types.
PMID:日期:2026-07-29To characterize deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein yields extracted from tissue of fourteen mouse organ types to establish baseline yield estimates for these biomolecules and provide guidance for tissue sample usage and storage in biomedical studies. Six wild-type C57BL/6J and six BALB/c nude mice were euthanized and fresh tissues were collected for extracting total DNA, RNA, and protein. The concentration, purity, and quality of nucleic acids were measured with NanoDrop™ One spectrophotometer, gel electrophoresis or capillary electrophoresis. Protein concentration was determined using the bicinchoninic acid (BCA) method. DNA yields were highest in the colorectum and lowest in bone. RNA yields were highest in the colorectum and spleen of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. Protein yields were highest in the kidney and liver of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. The tissue quantities required to obtain 10 μg of DNA, 10 μg of RNA, and 1 mg of protein were calculated and presented along with corresponding tissue sizes. DNA, RNA, and protein yields varied significantly across different mouse tissue types. These findings provide a reference for estimating tissue sample sizes needed for downstream assays and optimizing the preservation of mouse tissues.
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10. {"_":"Mitochondrial respiration and nucleotide profiling in human left internal mammary artery from CABG: a novel real-time approach.","i":["ex vivo"]}
10. {"_":"冠状动脉旁路移植术中人体左乳内动脉的线粒体呼吸与核苷酸谱分析：一种新的实时方法。","i":["离体"]}PMID:日期:2026-07-27[中文摘要] 线粒体功能障碍在心血管疾病和代谢性疾病的发病机制中发挥关键作用。然而，直接评估人血管组织中的线粒体呼吸在技术上仍具有挑战性。在本研究中，我们提出了一种对冠状动脉旁路移植术（CABG）中获取的人左乳内动脉（LIMA）移植物进行线粒体呼吸实时分析的方法。术中采集LIMA节段，并使用Seahorse XF Flex 3D分析仪进行生物能量学评估处理。采用线粒体应激测试评估线粒体呼吸，实现耗氧率（OCR）的实时测量。同时，使用高效液相色谱法（HPLC）定量组织核苷酸水平，从而对细胞能量状态和氧化还原平衡进行补充评估。我们证明了在完整人动脉组织中测量线粒体呼吸的可行性。据我们所知，这是Seahorse XF Flex 3D平台首次应用于完整人血管组织的实时生物能量学分析。OCR与核苷酸水平的联合分析实现了对血管生物能量学状态的综合评估。所应用的方案能够可靠评估关键生物能量学参数，包括基础呼吸和最大呼吸。本研究建立了一种用于人血管移植物生物能量学分析的新型概念验证工作流程，为未来心血管疾病和代谢性疾病中血管代谢的研究提供了平台。
[英文摘要] Mitochondrial dysfunction plays a critical role in the pathogenesis of cardiovascular and metabolic diseases. However, direct assessment of mitochondrial respiration in human vascular tissue remains technically challenging. In this study, we present an approach for real-time analysis of mitochondrial respiration in human left internal mammary artery (LIMA) grafts obtained during coronary artery bypass grafting (CABG). LIMA segment was collected intraoperatively and processed for bioenergetic assessment using the Seahorse XF Flex 3D Analyzer. Mitochondrial respiration was evaluated using the Mito Stress Test, enabling real-time measurement of oxygen consumption rate (OCR). In parallel, tissue nucleotide levels were quantified using high-performance liquid chromatography (HPLC), allowing complementary assessment of cellular energy status and redox balance. We demonstrate the feasibility of measuring mitochondrial respiration in intact human arterial tissue . To our knowledge, this is the first application of the Seahorse XF Flex 3D platform for real-time bioenergetic analysis in intact human vascular tissue. Combined analysis of OCR and nucleotide levels enabled integrated assessment of vascular bioenergetic status. The applied protocol enabled reliable assessment of key bioenergetic parameters, including basal and maximal respiration. This study establishes a novel proof-of-concept workflow for bioenergetic profiling of human vascular grafts, providing a platform for future investigations of vascular metabolism in cardiovascular and metabolic disorders.