石蜡包埋组织上的雄激素受体免疫组织化学检测

摘要(中文译文)

雄激素剥夺仍是转移性前列腺癌患者的主要治疗方法。然而，对激素调控的反应差异很大。雄激素受体（androgen receptor，AR）表达已被用于预测对抗雄激素治疗的临床反应。然而，AR表达的检测方法一直局限于对冷冻组织匀浆的胞质或核组分进行受体生化测定，而这种方法存在非恶性上皮细胞和间质细胞污染的明显问题。因此，将AR表达与激素治疗反应相关联的研究一直有限且存在争议。近年来，免疫组织化学分析方法已经问世，但仅能在冷冻组织上进行。我们描述了一种简便方法，利用抗原修复技术在福尔马林固定、石蜡包埋的组织切片上评估AR。原发性和转移性前列腺癌均显示AR核染色。在增生性和正常前列腺腺体中，分泌细胞呈均匀染色。大多数间质细胞具有强核阳性。所有其他受检癌症（结肠癌、乳腺癌、肺癌、皮肤癌、肾癌）均为阴性。这种免疫组织化学技术使得在常规处理的组织中评估前列腺癌AR状态成为可能。因此，将该技术应用于存档材料应能评估AR表达是否能预测晚期前列腺癌对内分泌治疗的反应。

Abstract

Androgen deprivation remains the primary therapy for patients with metastatic prostate cancer. Response to hormonal manipulation, however, has been quite variable. Androgen receptor (AR) expression has been used to predict clinical response to antiandrogenic treatment. However, methods of detection of AR expression have been limited to receptor biochemical assays in cytosolic or nuclear fractions of frozen tissue homogenates with obvious contamination problems by nonmalignant epithelial and stromal cells. As a result, studies correlating AR expression with response to hormonal therapy have been limited and controversial. More recently, immunohistochemical methods of analysis have become available, but only on frozen tissue. We describe a simple method to evaluate ARs on formalin-fixed, paraffin-embedded tissue sections using antigen retrieval methods. Primary, as well as metastatic, prostate carcinomas showed nuclear staining for ARs. Secretory cells stained uniformly in hyperplastic and normal prostatic glands. The majority of stromal cells had strong nuclear positivity. All other cancers tested (colon, breast, lung, skin, kidney) were negative. This immunohistochemical technique allows evaluation of prostate cancer AR status in routinely processed tissues. Thus, application of this technology to archival material should permit assessment of whether AR expression is predictive of response to endocrine therapy in advanced prostate cancer.

如何引用

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 的其他文献

• 多种癌细胞中WT1蛋白的免疫组化检测
• 缺乏证据表明EB病毒在肺和胸膜肿瘤中具有病因学作用