红树林来源真菌Quambalaria cyanescens的化学成分及其抗菌活性
Chemical constituents from the mangrove-derived fungus Quambalaria cyanescens and their antibacterial activity.
摘要(中文译文)
弧菌病对水产养殖构成重大细菌性威胁，影响多种鱼类和贝类。本研究从红树林来源真菌Quambalaria cyanescens HLLP-241中分离得到一个新的二苯并[b,f]氧杂䓬类似物phylloplaoxepin A（1）、四个新的1,4-萘醌类似物phylloplaquinones A–D（2–5），以及两个已知化合物6和7。化合物4和6对溶藻弧菌（V. alginolyticus）表现出强效抑制作用，两者MIC值均为3.125 μg/mL（氯霉素MIC=1.56 μg/mL）。化合物3和5–7对副溶血弧菌（V. parahaemolyticus）表现出显著抑制作用，MIC值均为0.78 μg/mL，与阳性对照相当（氯霉素MIC=0.78 μg/mL）。机制研究表明，化合物4以剂量依赖方式抑制溶藻弧菌生物膜形成，同时破坏膜完整性、耗竭ATP并诱导活性氧（ROS）蓄积。转录组分析、qRT-PCR验证、分子对接、分子动力学模拟及酶学实验共同证明，SerA是化合物4抑制溶藻弧菌生长的关键功能靶点。
Abstract
Vibriosis poses a significant bacterial threat to aquaculture, affecting a diverse range of fish and shellfish. In this study, a novel dibenzo[b,f]oxepin analogue, phylloplaoxepin A (1), and four new 1,4-naphthoquinone analogues, phylloplaquinones A-D (2-5), along with two known compounds 6 and 7 were isolated from the mangrove-derived fungus Quambalaria cyanescens HLLP-241. Compounds 4 and 6 exhibited potent inhibition against V. alginolyticus, with both exhibiting a MIC values of 3.125 (chloramphenicol, MIC = 1.56 μg/mL). Compounds 3 and 5-7 exhibited significant inhibitory effects against V. parahaemolyticus, each with a MIC values of 0.78 μg/mL, comparable to the positive control (chloramphenicol, MIC = 0.78 μg/mL). Mechanistic studies reveal that compound 4 inhibits biofilm formation of V. alginolyticus in a dose-dependent manner, while simultaneously disrupting membrane integrity, depleting ATP, and inducing accumulation of reactive oxygen species (ROS). Transcriptomic analysis, qRT-PCR validation, molecular docking, molecular dynamics simulations, and enzymatic assays collectively demonstrate that SerA is the key functional target of compound 4 in inhibiting the growth of V. alginolyticus.
如何引用
AMA
Hong Xiao, Pei Lv, Xiaobo Liu, Jingyu Yang, Bin Gong, Tiantao Xiong, et al. Chemical constituents from the mangrove-derived fungus Quambalaria cyanescens and their antibacterial activity.. Bioorganic chemistry. 2026; doi:10.1016/j.bioorg.2026.110382.
APA
Hong Xiao, Pei Lv, Xiaobo Liu, Jingyu Yang, Bin Gong, Tiantao Xiong, et al (2026). Chemical constituents from the mangrove-derived fungus Quambalaria cyanescens and their antibacterial activity.. Bioorganic chemistry. https://doi.org/10.1016/j.bioorg.2026.110382
GB/T 7714
Hong Xiao, Pei Lv, Xiaobo Liu, Jingyu Yang, Bin Gong, Tiantao Xiong, et al. Chemical constituents from the mangrove-derived fungus Quambalaria cyanescens and their antibacterial activity.[J]. Bioorganic chemistry, 2026 doi:10.1016/j.bioorg.2026.110382.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出