Fusaspironols A-E: Steroidal spirostanols from the deep-sea-derived Fusarium inflexum ZEN8 with inhibitory activities against osteoclastogenesis and adipogenic differentiation.
从深海来源的尖孢镰刀菌ZEN8（Fusarium inflexum ZEN8）中分离得到五个此前未描述的多羟基螺甾烷醇类化合物fusaspironols A–E（1–5），以及八个已知化合物（6–13）。通过光谱分析、基于GIAO的NMR计算和DP4+概率分析阐明了它们的结构。值得注意的是，这是真菌来源螺甾烷醇类化合物的第二次报道。提出了连接化合物1–5与该真菌中假定前体胆固醇的合理生物合成途径。在生物学实验中，化合物8显著抑制了骨髓巨噬细胞中RANKL诱导的破骨细胞生成，而化合物2、3和7则抑制了成熟骨髓基质细胞的脂肪生成。在测试浓度下，所有化合物均未表现出可检测的细胞毒性，提示其作为抗骨质疏松药物开发先导化合物的潜力。
Five previously undescribed polyhydroxylated spirostanols, fusaspironols A-E (1-5), along with eight known compounds (6-13), were isolated from the deep-sea-derived fungus Fusarium inflexum ZEN8. Their structures were elucidated using spectroscopic analysis, GIAO-based NMR calculations, and DP4+ probability analysis. Notably, this represents only the second report of spirostanols of fungal origin. A plausible biosynthetic pathway linking compounds 1-5 to the putative precursor cholesterol in this fungus was proposed. In biological assays, compound 8 significantly inhibited RANKL-induced osteoclastogenesis in bone marrow macrophages, whereas compounds 2, 3, and 7 suppressed adipogenesis in mature bone marrow stromal cells. None of the compounds exhibited detectable cytotoxicity at the tested concentrations, suggesting their potential as lead compounds for anti-osteoporosis drug development.
Tai-Zong Wu, Wen-Si Su, Meng Zhang, Yan-Qing Guo, Hui-Ping Liu, You Li, et al. Fusaspironols A-E: Steroidal spirostanols from the deep-sea-derived Fusarium inflexum ZEN8 with inhibitory activities against osteoclastogenesis and adipogenic differentiation.. Bioorganic chemistry. 2026; doi:10.1016/j.bioorg.2026.110342.
Tai-Zong Wu, Wen-Si Su, Meng Zhang, Yan-Qing Guo, Hui-Ping Liu, You Li, et al (2026). Fusaspironols A-E: Steroidal spirostanols from the deep-sea-derived Fusarium inflexum ZEN8 with inhibitory activities against osteoclastogenesis and adipogenic differentiation.. Bioorganic chemistry. https://doi.org/10.1016/j.bioorg.2026.110342
Tai-Zong Wu, Wen-Si Su, Meng Zhang, Yan-Qing Guo, Hui-Ping Liu, You Li, et al. Fusaspironols A-E: Steroidal spirostanols from the deep-sea-derived Fusarium inflexum ZEN8 with inhibitory activities against osteoclastogenesis and adipogenic differentiation.[J]. Bioorganic chemistry, 2026 doi:10.1016/j.bioorg.2026.110342.
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