磁性镍纳米颗粒的制备及其在高效纯化组氨酸标记的融合蛋白中的应用
Preparation of Magnetic Nickel Nanoparticles and Their Use in Efficient Purification of Histidine-Tagged Fusion Proteins.
摘要(中文译文)
镍纳米颗粒（记为Ni NPs）可应用于催化、电池制造和生物分离等领域，因其显著的磁性、晶体各向异性、高矫顽力和优异的化学稳定性而备受关注。与以往需要通过多步表面功能化修饰螯合配体（如次氮基三乙酸，缩写为NTA）来实现组氨酸标签（记为His-tagged）蛋白结合的研究不同，本研究报道了一种一步水热合成具有球形-尖刺结构的Ni NPs的方法，该结构可提供丰富的内在Ni2+活性位点。通过一系列合成实验系统优化了水热反应条件（溶液pH、温度和时间）。结合其丰富的Ni2+活性结合位点和强磁响应性，评估了Ni NPs分离和纯化His-tagged融合蛋白的能力。结果发现，Ni NPs能够快速、特异且高效地纯化多种His-tagged蛋白，达到80.36 mg/g的高饱和吸附容量，显著高于商业Ni-NTA树脂和此前报道的镍基吸附剂，同时保持优异的可循环使用性（5次循环后仍大于80%）。这些特性凸显了Ni NPs作为低成本、可重复使用且高性能的蛋白质分离纳米吸附剂的潜力，相比传统依赖功能化的方法具有明显优势。
Abstract
Nickel nanoparticles (denoted as Ni NPs), being applicable in catalysis, battery manufacturing, and bioseparation, have garnered considerable attention due to their remarkable magnetism, crystalline anisotropy, high coercivity, and excellent chemical stability. Unlike previous studies that require multi-step surface functionalization with chelating ligands, such as nitrilotriacetic acid (abridged as NTA), to achieve histidine-tagged (denoted as His-tagged) protein binding, this study reports a one-step hydrothermal synthesis of Ni NPs with a spherical-spiky architecture that provides abundant intrinsic Ni2+ active sites. A series of synthesis experiments were conducted to systematically optimize the hydrothermal reaction conditions (solution pH, temperature, and time). The ability of Ni NPs to separate and purify His-tagged fusion proteins was evaluated in relation to their abundant Ni2+ active binding sites and robust magnetic responsiveness. It was found that Ni NPs were able to rapidly, specifically, and efficiently purify a variety of His-tagged proteins, achieving a high saturation adsorption capacity of 80.36 mg/g, significantly higher than commercial Ni-NTA resins and previously reported Ni-based adsorbents, while retaining excellent recyclability (>80% after 5 cycles). These features underscore the potential of Ni NPs as cost-effective, reusable, and high-performance nano-adsorbents for protein separation, offering distinct advantages over conventional functionalization-dependent methods.
如何引用
AMA
Mochou Gao, Xing Miao, Haowen Ren, Haocheng Xu, Haotian Li, Hao Wang, et al. Preparation of Magnetic Nickel Nanoparticles and Their Use in Efficient Purification of Histidine-Tagged Fusion Proteins.. ACS applied bio materials. 2026; doi:10.1021/acsabm.6c00399.
APA
Mochou Gao, Xing Miao, Haowen Ren, Haocheng Xu, Haotian Li, Hao Wang, et al (2026). Preparation of Magnetic Nickel Nanoparticles and Their Use in Efficient Purification of Histidine-Tagged Fusion Proteins.. ACS applied bio materials. https://doi.org/10.1021/acsabm.6c00399
GB/T 7714
Mochou Gao, Xing Miao, Haowen Ren, Haocheng Xu, Haotian Li, Hao Wang, et al. Preparation of Magnetic Nickel Nanoparticles and Their Use in Efficient Purification of Histidine-Tagged Fusion Proteins.[J]. ACS applied bio materials, 2026 doi:10.1021/acsabm.6c00399.
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