猪源乳酸菌的体外益生特性及全基因组测序分析
{"_":" probiotic characteristics and whole-genome sequencing analysis of porcine-derived lactic acid bacteria.","i":["In vitro"]}
摘要(中文译文)
乳酸菌（LAB）具有作为益生菌的应用潜力，但其携带的抗生素耐药基因（ARGs）和毒力因子基因（VFGs）构成显著的公共卫生风险。本研究从规模化养猪场粪便样本中分离出八株乳酸菌，并通过体外功能实验和全基因组测序系统评价其益生潜力与安全性。结果表明，R124对肠上皮细胞表现出高黏附率（54.11%）和较强的共聚集能力（34.9%）。Z108表现出较强的耐酸性（pH 2.0时存活率为24.18%），而F130对高浓度胆盐的耐受性最高（6.28%）。所有菌株均呈现非溶血性γ-溶血表型，但抗生素敏感性试验揭示了广泛的表型多重耐药。值得注意的是，尽管R124和Z108对某些抗生素表现出耐药性，基因组分析表明它们不携带获得性ARGs或VFGs，提示其观察到的耐药性可能源于内在机制。F130携带四个VFGs和十三个ARGs，其中八个位于质粒上。重要的是，滤膜交配实验表明，F130的耐药质粒PlasmidE及其相关耐药基因可水平转移至受体菌株，赋予其抗生素耐药性。本研究表明，尽管所有分离菌株均具有一定的益生潜力，但其安全性因菌种和菌株特异性特征而异。R124和Z108兼具功能安全性和遗传安全性，是有前景的候选菌株，而F130的耐药性传播风险凸显了在益生菌筛选中进行全基因组安全性评估的必要性。
Abstract
Lactic acid bacteria (LAB) have potential applications as probiotics, but the antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) they carry pose significant public health risks. In this study, eight strains of LAB were isolated from fecal samples of large-scale pig farms, and their probiotic potential and safety were systematically evaluated through in vitro functional assays and whole-genome sequencing. The results showed that R124 exhibited high adhesion to intestinal epithelial cells (54.11%) and strong coaggregation ability (34.9%). Z108 demonstrated relatively strong acid resistance (24.18% survival rate at pH 2.0), while F130 showed the highest tolerance to high concentrations of bile salts (6.28%). All strains exhibited a non-hemolytic γ -hemolysis phenotype, but antibiotic susceptibility testing revealed widespread phenotypic multidrug resistance. Interestingly, while R124 and Z108 showed resistance to certain antibiotics, genomic analysis indicated they harbored no acquired ARGs or VFGs, suggesting that their observed resistance may stem from intrinsic mechanisms. F130 harbored four VFGs and thirteen ARGs, eight of which were located on plasmids. Importantly, the filter-mating experiment indicated that the antibiotic-resistant plasmid PlasmidE and its associated resistance gene from F130 could be horizontally transferred to recipient strains, conferring antibiotic resistance. This study suggests that, while all isolated strains possess certain probiotic potential, their safety varies according to species and strain-specific characteristics. R124 and Z108 are promising candidates with both functional and genetic safety, while the risk of resistance spread from F130 highlights the necessity of whole-genome safety assessments in probiotic screening.
如何引用
AMA
Shumei Li, Heming Zhang, Xin Xing, Yuyang Wang, Ying Shao, Zhao Qi. {"_":" probiotic characteristics and whole-genome sequencing analysis of porcine-derived lactic acid bacteria.","i":["In vitro"]}. PeerJ. 2026; doi:10.7717/peerj.21496.
APA
Shumei Li, Heming Zhang, Xin Xing, Yuyang Wang, Ying Shao, Zhao Qi (2026). {"_":" probiotic characteristics and whole-genome sequencing analysis of porcine-derived lactic acid bacteria.","i":["In vitro"]}. PeerJ. https://doi.org/10.7717/peerj.21496
GB/T 7714
Shumei Li, Heming Zhang, Xin Xing, Yuyang Wang, Ying Shao, Zhao Qi. {"_":" probiotic characteristics and whole-genome sequencing analysis of porcine-derived lactic acid bacteria.","i":["In vitro"]}[J]. PeerJ, 2026 doi:10.7717/peerj.21496.
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