冠状病毒NSP13解旋酶功能研究的最新进展和抑制剂开发的挑战
Recent advances in functional studies of coronavirus NSP13 helicase and challenges in inhibitor development.
摘要(中文译文)
冠状病毒解旋酶NSP13对病毒复制和转录至关重要，因其高度序列保守性和结构同源性，是广谱抗冠状病毒药物的有前景靶点。本综述总结了七种感染人类的冠状病毒中NSP13的序列特征、结构组织和功能活性。我们概述了关键酶学特性，包括双链RNA/DNA解旋和NTP水解，并描述了NSP13如何与其他非结构蛋白协同驱动复制和转录。除经典解旋酶作用外，我们讨论了冠状病毒中G-四链体（G4）元件的基因组分布，以及G4结构与NSP13在调控病毒生命周期中的潜在功能联系。最后，我们重点介绍了靶向NSP13抑制剂开发的最新进展，并探讨了其用于COVID-19和其他新发冠状病毒的潜在价值，为广谱抗病毒设计提供了依据。
Abstract
Coronavirus helicase NSP13 is essential for viral replication and transcription and is a promising target for broad-spectrum anti-coronavirus drugs due to its high sequence conservation and structural homology. This review summarizes NSP13 sequence features, structural organization, and functional activities across the seven human-infecting coronaviruses. We outline key enzymatic properties, including duplex RNA/DNA unwinding and NTP hydrolysis, and describe how NSP13 cooperates with other nonstructural proteins to drive replication and transcription. Beyond canonical helicase roles, we discuss the genomic distribution of G-quadruplex (G4) elements in coronaviruses and potential functional connections between G4 structures and NSP13 in regulating the viral life cycle. Finally, we highlight recent progress in developing NSP13-targeting inhibitors and consider their potential utility against COVID-19 and other emerging coronaviruses, providing a rationale for broad-spectrum antiviral design.
如何引用
AMA
Yangxue Dai, Silan Li, Xue He, Keyu Lu, Junjie Tian, Yuanmou Huang, et al. Recent advances in functional studies of coronavirus NSP13 helicase and challenges in inhibitor development.. Virulence. 2026; doi:10.1080/21505594.2026.2696653.
APA
Yangxue Dai, Silan Li, Xue He, Keyu Lu, Junjie Tian, Yuanmou Huang, et al (2026). Recent advances in functional studies of coronavirus NSP13 helicase and challenges in inhibitor development.. Virulence. https://doi.org/10.1080/21505594.2026.2696653
GB/T 7714
Yangxue Dai, Silan Li, Xue He, Keyu Lu, Junjie Tian, Yuanmou Huang, et al. Recent advances in functional studies of coronavirus NSP13 helicase and challenges in inhibitor development.[J]. Virulence, 2026 doi:10.1080/21505594.2026.2696653.
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