整合的单细胞和空间转录组学方法，以解读癌症的肿瘤微环境和治疗耐药性
Integrative single-cell and spatial transcriptomic approaches to decipher the tumor microenvironment and therapeutic resistance in pancreatic cancer.
摘要(中文译文)
胰腺导管腺癌（PDAC）是人类最具侵袭性的恶性肿瘤之一，预后极差。其进展在很大程度上由高度复杂且具有免疫抑制性的肿瘤微环境（TME）所驱动，这凸显了深入理解其分子机制的迫切需求。近年来，单细胞RNA测序（scRNA-seq）和空间转录组学（ST）的进展为剖析TME内的细胞异质性、空间组织和基因表达动态提供了前所未有的机遇。在本综述中，我们总结了主要的scRNA-seq和ST技术及其在癌症研究中的独特优势，并重点介绍了它们在揭示PDAC异质性、基质-免疫相互作用和治疗耐药机制方面的整合应用。我们进一步讨论了这些方法如何为生物标志物的发现提供信息，并指导新型治疗策略的开发。总之，这些发现表明，整合单细胞和空间转录组学为推进精准肿瘤学和改善胰腺癌患者预后提供了变革性的潜力。
Abstract
Pancreatic ductal adenocarcinoma (PDAC) is among the most aggressive human malignancies and has an extremely poor prognosis. Its progression is largely driven by a highly complex and immunosuppressive tumor microenvironment (TME), highlighting the urgent need for a deeper understanding of its molecular mechanisms. Recent advances in single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have provided unprecedented opportunities to dissect cellular heterogeneity, spatial organization, and gene expression dynamics within the TME. In this review, we summarize the major scRNA-seq and ST technologies and their unique strengths in cancer research and highlight their integrated applications in revealing PDAC heterogeneity, stromal-immune interactions, and mechanisms of therapeutic resistance. We further discuss how these approaches can inform biomarker discovery and guide the development of novel therapeutic strategies. Together, these findings suggest that integrated single-cell and spatial transcriptomics offers transformative potential to advance precision oncology and improve outcomes for patients with pancreatic cancer.
如何引用
AMA
Mengting Luo, Feng Shen, Wanli Xu, Christopher Corpe, Jin Wang. Integrative single-cell and spatial transcriptomic approaches to decipher the tumor microenvironment and therapeutic resistance in pancreatic cancer.. Cancer biology & therapy. 2026; doi:10.1080/15384047.2026.2699508.
APA
Mengting Luo, Feng Shen, Wanli Xu, Christopher Corpe, Jin Wang (2026). Integrative single-cell and spatial transcriptomic approaches to decipher the tumor microenvironment and therapeutic resistance in pancreatic cancer.. Cancer biology & therapy. https://doi.org/10.1080/15384047.2026.2699508
GB/T 7714
Mengting Luo, Feng Shen, Wanli Xu, Christopher Corpe, Jin Wang. Integrative single-cell and spatial transcriptomic approaches to decipher the tumor microenvironment and therapeutic resistance in pancreatic cancer.[J]. Cancer biology & therapy, 2026 doi:10.1080/15384047.2026.2699508.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出