[弱光胁迫对半夏生长及有效成分含量的影响]
[Effect of low light stress on growth and active ingredient content of Pinellia ternata].
摘要(中文译文)
半夏是一种典型的耐阴植物。本研究采用LED光源，探究了半夏幼苗在三种光照强度下的表型、生理及分子响应：过度弱光（L1，100 μmol·m~(-2)·s~(-1)）、弱光（L2，200 μmol·m~(-2)·s~(-1)）和临界光强（L3，400 μmol·m~(-2)·s~(-1)）。结果表明，株高和叶绿素含量在L2处理下最高，而叶面积和生物量在L3处理下最高。L2处理下生物活性化合物含量高于其他两个光照处理。相反，过度遮阴（L1）因净光合速率较低而显著抑制了幼苗株高和茎粗，并诱导了较高的膜脂过氧化。此外，总生物碱和核苷含量在L3处理下最高，而总有机酸含量在L2处理下最高。进一步研究发现，处理5天时，光合效率及相关指标在L3处理下最高；而处理20天时，光合参数在L2处理下升高。转录组分析显示，响应不同光照强度的差异表达基因（DEGs）显著富集于光合作用、光合天线蛋白、苯丙烷生物合成以及淀粉和蔗糖代谢等相关通路。其中，42个DEGs参与光合作用相关通路，通过光系统相关基因（psa、psb、PNSL）、光合电子传递基因（petA、petC、petD）和腺苷三磷酸（ATP）合酶（atp、ATPC、ATPF2）调控光合效率。这些基因大多在处理5天时的L3和处理20天时的L2处理下上调表达。综上，这些发现阐明了半夏幼苗响应不同遮阴水平的生理和分子机制，为理解该物种的光照调控、栽培及生态适应机制提供了依据。
Abstract
Pinellia ternata is a characteristic shade-tolerant plant. In this study, LED light sources were used to investigate the phenotypic, physiological, and molecular responses of P. ternata seedlings under three light intensities: excessively low light(L1, 100 μmol·m~(-2)·s~(-1)), low light(L2, 200 μmol·m~(-2)·s~(-1)), and critical light(L3, 400 μmol·m~(-2)·s~(-1)). The results showed that plant height and chlorophyll content were highest under L2, while leaf area and biomass were highest under L3. The content of bioactive compounds was higher under L2 than under the other two light treatments. In contrast, excessive shading(L1) significantly inhibited seedling height and stem diameter due to a lower net photosynthetic rate and induced higher membrane lipid peroxidation. Additionally, the highest total alkaloid and nucleoside contents were observed under L3, whereas the highest total organic acid content was found under L2. Furthermore, at 5 days of treatment, photosynthetic efficiency and related indicators were highest under L3, whereas at 20 days, photosynthetic parameters increased under L2. Transcriptome analysis revealed that differentially expressed genes(DEGs) in response to different light intensities were significantly enriched in pathways related to photosynthesis, photosynthetic-antenna proteins, phenylpropanoid biosynthesis, and starch and sucrose metabolism. Among these, 42 DEGs were involved in photosynthesis-related pathways, regulating photosynthetic efficiency through photosystem related genes(psa, psb, PNSL), photosynthetic electron transport genes(petA, petC, petD), and adenosine triphosphate(ATP) synthase(atp, ATPC, ATPF2). Most of these genes were upregulated under L3 at 5 days and under L2 at 20 days. Together, these findings elucidate the physiological and molecular mechanisms by which P. ternata seedlings respond to different shading levels, providing a basis for understanding light regulation, cultivation, and ecological adaptation mechanisms in this species.
如何引用
AMA
Xue-Jiao Wen, Bin-Bin Liao, Jiao Fu, Xin-Yao Li, Hu Luo, Da-Hui Liu, et al. [Effect of low light stress on growth and active ingredient content of Pinellia ternata].. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2026; doi:10.19540/j.cnki.cjcmm.20251103.402.
APA
Xue-Jiao Wen, Bin-Bin Liao, Jiao Fu, Xin-Yao Li, Hu Luo, Da-Hui Liu, et al (2026). [Effect of low light stress on growth and active ingredient content of Pinellia ternata].. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. https://doi.org/10.19540/j.cnki.cjcmm.20251103.402
GB/T 7714
Xue-Jiao Wen, Bin-Bin Liao, Jiao Fu, Xin-Yao Li, Hu Luo, Da-Hui Liu, et al. [Effect of low light stress on growth and active ingredient content of Pinellia ternata].[J]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2026 doi:10.19540/j.cnki.cjcmm.20251103.402.
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