电沉积磷酸钙涂层对骨植入物早期炎症反应的免疫调节作用
Immunomodulatory effect of electrodeposited calcium phosphate coatings on early inflammatory response to bone implant.
摘要(中文译文)
宿主细胞对生物材料的反应对于植入物在骨部位的长期整合至关重要。磷酸钙涂层，尤其是羟基磷灰石（HA）涂层，改善了钛植入物的生物学性能和骨整合。然而，在更好地控制无菌性松动风险方面仍需取得进展，该风险主要与植入物附近不受控制的炎症反应有关。与HA相比，缺钙羟基磷灰石（CDHA）已表现出更优越的生物活性和体内性能。本研究的目的是在体外确定三种不同植入物表面——蚀刻钛合金、HA涂层钛和CDHA涂层钛——对人原代单核细胞早期炎症反应及其向破骨细胞分化的影响，以此作为植入物命运的预测指标。ELISA和RT-qPCR证实，与CDHA相比，HA引起更高的细胞因子反应（IL-8、TNF-α、IL-1β、IL-6）。酶谱实验显示，CDHA具有适度的MMP-9蛋白水解特征，这可能有利于植入物周围的骨重塑。在两种涂层存在下均未观察到破骨细胞生成增加，表明免疫细胞活化并不必然导致破骨细胞介导的骨降解。本研究表明，钛植入物上的CDHA涂层比标准HA涂层引发更合适的免疫反应，降低了炎症性骨溶解的风险。这些数据还强调，为准确预测植入物预后，需要考虑整个植入物周围环境。
Abstract
Host cells response to biomaterials is crucial for implant long-term integration in bone site. Calcium phosphate coatings, particularly hydroxyapatite (HA) coatings, have improved the biological properties and osseointegration of titanium implants. However, progress still needs to be made in better controlling the risk of aseptic loosening, which is mainly associated with uncontrolled inflammatory reaction near the implant. Calcium-deficient hydroxyapatite (CDHA) has demonstrated superior bioactivity and in vivo performance compared to HA. The aim of this work was to determine in vitro the effects of three different implant surfaces, etched titanium alloy, HA-coated titanium and CDHA-coated titanium on the early inflammatory response of human primary monocytes and their differentiation into osteoclasts as a predictor of implant fate. ELISA and RT-qPCR evidenced a higher cytokine response (IL-8, TNF-α, IL-1β, IL-6) with HA compared to CDHA. Zymography experiments revealed a moderate MMP-9 proteolytic profile with CDHA, which could be conducive to bone remodeling around the implant. No increased osteoclastogenesis was observed in the presence of both coatings, indicating that immune cell activation does not necessarily lead to osteoclast-mediated bone degradation. This study showed that CDHA coating on a titanium implant elicits a more suitable immune response than standard HA coating, reducing the risk of inflammatory osteolysis. These data also highlighted the need to consider the whole peri-implant environment for accurate implant prognosis.
如何引用
AMA
Léa Thoraval, Richard Drevet, Christine Guillaume, Camille Bour, Hicham Benhayoune, Frédéric Velard. Immunomodulatory effect of electrodeposited calcium phosphate coatings on early inflammatory response to bone implant.. Biomaterials advances. 2026; doi:10.1016/j.bioadv.2026.215043.
APA
Léa Thoraval, Richard Drevet, Christine Guillaume, Camille Bour, Hicham Benhayoune, Frédéric Velard (2026). Immunomodulatory effect of electrodeposited calcium phosphate coatings on early inflammatory response to bone implant.. Biomaterials advances. https://doi.org/10.1016/j.bioadv.2026.215043
GB/T 7714
Léa Thoraval, Richard Drevet, Christine Guillaume, Camille Bour, Hicham Benhayoune, Frédéric Velard. Immunomodulatory effect of electrodeposited calcium phosphate coatings on early inflammatory response to bone implant.[J]. Biomaterials advances, 2026 doi:10.1016/j.bioadv.2026.215043.
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