电针可缓解骨关节炎，并与Nrf2/HO-1通路的激活和铁下垂相关变化的减少有关
Electroacupuncture alleviates osteoarthritis and is associated with activation of the Nrf2/HO-1 pathway and reduced ferroptosis-related changes.
摘要(中文译文)
电针（EA）在骨关节炎（OA）中已显示出有益作用，但其潜在机制仍不清楚。本研究探讨了电针对OA的作用是否与铁死亡相关标志物及Nrf2/HO-1信号通路的变化有关。将大鼠随机分为对照组、OA组、OA+ferrostatin-1组、OA+电针组和OA+ferrostatin-1+电针组。评估大体软骨外观、缩足阈值、体重和膝关节宽度。通过HE染色、番红O-固绿染色和OARSI评分评估软骨损伤。采用ELISA法检测血清和滑液中的炎性细胞因子。采用TUNEL染色、免疫荧光、生化检测和western blot评估软骨组织中的细胞凋亡、铁死亡相关标志物及Nrf2/HO-1信号通路。与对照组相比，OA大鼠表现出明显的软骨损伤、缩足阈值降低、体重增长减少以及膝关节宽度增加。OA组的组织学损伤和OARSI评分显著升高。血清和滑液中IL-6、IL-1β和TNF-α水平也升高。在软骨组织中，OA与细胞凋亡增加、SLC7A11、GPX4、SOX9和GSH水平降低以及Fe和ACSL4水平升高相关。OA软骨中Nrf2和HO-1表达增加。ferrostatin-1、电针或联合治疗均不同程度地减轻了上述变化。电针可减轻OA相关的结构和炎症改变，并与铁死亡相关改变的减少及Nrf2/HO-1信号通路的增强相关。
Abstract
Electroacupuncture (EA) has shown beneficial effects in osteoarthritis (OA), but the underlying mechanism remains unclear. This study examined whether the effects of EA on OA are associated with changes in ferroptosis-related markers and Nrf2/HO-1 signaling. Rats were randomly assigned to the Control, OA, OA + ferrostatin-1, OA + EA, and OA + ferrostatin-1+EA groups. Gross cartilage appearance, paw withdrawal threshold, body weight, and knee joint width were evaluated. Cartilage injury was assessed by HE staining, Safranin O-fast green staining, and OARSI scoring. Inflammatory cytokines in serum and synovial fluid were measured by ELISA. TUNEL staining, immunofluorescence, biochemical assays, and western blot were used to assess apoptosis, ferroptosis-related markers, and Nrf2/HO-1 signaling in cartilage tissue. Compared with the Control group, OA rats showed obvious cartilage damage, lower paw withdrawal threshold, reduced body weight gain, and increased knee joint width. Histological injury and OARSI scores were markedly increased in the OA group. Serum and synovial fluid levels of IL-6, IL-1β, and TNF-α were also elevated. In cartilage tissue, OA was associated with increased apoptosis, decreased SLC7A11, GPX4, SOX9, and GSH levels, and increased Fe and ACSL4 levels. Nrf2 and HO-1 expression was increased in OA cartilage. Treatment with ferrostatin-1, EA, or the combined treatment alleviated these changes to different degrees. EA alleviated OA-related structural and inflammatory changes and was associated with reduced ferroptosis-related alterations and enhanced Nrf2/HO-1 signaling.
如何引用
AMA
Mei Zhao, Lingxing Ouyang, Junjie Ma, Huimeng Zhu, Shengfang Zhu, Xuan Zhou, et al. Electroacupuncture alleviates osteoarthritis and is associated with activation of the Nrf2/HO-1 pathway and reduced ferroptosis-related changes.. Frontiers in molecular biosciences. 2026; doi:10.3389/fmolb.2026.1764620.
APA
Mei Zhao, Lingxing Ouyang, Junjie Ma, Huimeng Zhu, Shengfang Zhu, Xuan Zhou, et al (2026). Electroacupuncture alleviates osteoarthritis and is associated with activation of the Nrf2/HO-1 pathway and reduced ferroptosis-related changes.. Frontiers in molecular biosciences. https://doi.org/10.3389/fmolb.2026.1764620
GB/T 7714
Mei Zhao, Lingxing Ouyang, Junjie Ma, Huimeng Zhu, Shengfang Zhu, Xuan Zhou, et al. Electroacupuncture alleviates osteoarthritis and is associated with activation of the Nrf2/HO-1 pathway and reduced ferroptosis-related changes.[J]. Frontiers in molecular biosciences, 2026 doi:10.3389/fmolb.2026.1764620.
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