植物鞘氨醇破坏双物种口腔生物膜，并减弱LPS诱导的人牙龈成纤维细胞的促炎反应
Phytosphingosine disrupts dual-species oral biofilms and attenuates LPS-induced pro-inflammatory responses in human gingival fibroblasts.
摘要(中文译文)
口腔生物膜是龋病和牙周炎的主要病因因素，且通常对常规抗微生物治疗具有耐药性，尤其是在混合菌种群落中。植物鞘氨醇（PHS）是一种天然存在的生物活性鞘脂，已证实对多种病原体具有抗微生物活性；然而，其对混合菌种口腔生物膜及炎症反应的影响仍不明确。本研究探讨了PHS对单菌种和双菌种生物膜中变形链球菌和伴放线聚集杆菌的体外抗微生物、抗生物膜及免疫调节活性。共聚焦激光扫描显微镜显示，与未处理对照组相比，经PHS处理的单菌种和双菌种生物膜中生物膜基质形成均显著减少（P < 0.001）。细胞毒性分析表明，暴露于5 µg/ml PHS 24小时后，细胞活力仍保持在90%以上。此外，PHS预处理显著减弱了LPS诱导的IL-6和TNF-α产生（P < 0.001）。综上所述，这些发现表明PHS是一种具有抗微生物和抗炎特性的有前景的双功能制剂，可能作为生物膜相关口腔疾病的辅助治疗策略具有潜在应用价值。
Abstract
Oral biofilms are major etiological factors in dental caries and periodontitis and are often resistant to conventional anti-microbial therapies, particularly in mixed-species communities. Phytosphingosine (PHS), a naturally occurring bioactive sphingolipid, has demonstrated anti-microbial activity against several pathogens; however, its effects on mixed-species oral biofilms and inflammatory responses remain unclear. This study investigated the in vitro anti-microbial, anti-biofilm, and immunomodulatory activities of PHS against Streptococcus mutans and Aggregatibacter actinomycetemcomitans in single- and dual-species biofilms. Confocal laser scanning microscopy revealed a significant reduction in biofilm matrix formation in both single- and dual-species biofilms treated with PHS compared with untreated controls (P < 0.001). Cytotoxicity analysis demonstrated that cell viability remained above 90% following exposure to 5 µg/ml PHS for 24 h. Furthermore, PHS pretreatment significantly attenuated LPS-induced IL-6 and TNF-α production (P < 0.001). Collectively, these findings suggest that PHS is a promising dual-function agent with anti-microbial and anti-inflammatory properties and may have potential as an adjunctive therapeutic strategy for biofilm-associated oral diseases.
如何引用
AMA
Nattaset Chamnanvinijchai, Nicha Techawatcharathep, Natnicha Ngerndee, Sittikorn Teerawongwiwat, Boonjira Anukul, Panitan Jumjitvi, et al. Phytosphingosine disrupts dual-species oral biofilms and attenuates LPS-induced pro-inflammatory responses in human gingival fibroblasts.. Scientific reports. 2026; doi:10.1038/s41598-026-55714-3.
APA
Nattaset Chamnanvinijchai, Nicha Techawatcharathep, Natnicha Ngerndee, Sittikorn Teerawongwiwat, Boonjira Anukul, Panitan Jumjitvi, et al (2026). Phytosphingosine disrupts dual-species oral biofilms and attenuates LPS-induced pro-inflammatory responses in human gingival fibroblasts.. Scientific reports. https://doi.org/10.1038/s41598-026-55714-3
GB/T 7714
Nattaset Chamnanvinijchai, Nicha Techawatcharathep, Natnicha Ngerndee, Sittikorn Teerawongwiwat, Boonjira Anukul, Panitan Jumjitvi, et al. Phytosphingosine disrupts dual-species oral biofilms and attenuates LPS-induced pro-inflammatory responses in human gingival fibroblasts.[J]. Scientific reports, 2026 doi:10.1038/s41598-026-55714-3.
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