黄颡鱼两个亚种的线粒体基因组：对Siphonaptera内分子进化和系统发育分析的见解
{"_":"Mitochondrial genomes of two subspecies of : Insights into the molecular evolution and phylogenetic analysis within Siphonaptera.","i":["Citellophilus tesquorum"]}
摘要(中文译文)
在中国古北区的自然鼠疫疫源地中，某种蚤是主要的媒介蚤种，对兽医和公共卫生构成重大威胁。对其亚种进行精确鉴定和可靠的分类学界定，对于有效的媒介监测和进化研究至关重要。我们首先进行形态学鉴定以确保标本的真实性和准确性，随后利用Illumina NovaSeq平台对某蚤和某蚤的完整线粒体基因组进行了测序和注释。结合33个公开可用的蚤类线粒体基因组进行了比较线粒体基因组学和系统发育分析，以探讨基因组进化、密码子使用模式及系统发育关系。两个新测序的线粒体基因组表现出高度结构保守性和强烈的AT偏好，与蚤目（Siphonaptera）的典型特征一致。自然选择被确定为驱动密码子使用偏好的主导力量。系统发育重建显示，某蚤与某蚤紧密聚类，而某蚤形成了支持度良好的姐妹支系，表明这些亚种之间存在遗传分化。观察到的遗传分化可能由长期地理隔离和宿主适应所驱动。本研究建立了形态学与线粒体基因组学相结合的综合框架，丰富了蚤类线粒体基因组资源，并为蚤类媒介的系统发育、分类学及监测提供了关键分子证据。
Abstract
is a major flea vector in natural plague foci across the Palaearctic region of China, representing a significant threat to veterinary and public health. Precise identification and sound taxonomic delimitation of its subspecies are critical for effective vector surveillance and evolutionary research. We first performed morphological identification to guarantee specimen authenticity and accuracy, then sequenced and annotated the complete mitochondrial genomes of and using the Illumina NovaSeq platform. Comparative mitogenomic and phylogenetic analyses were conducted with 33 publicly available flea mitochondrial genomes to explore genomic evolution, codon usage patterns, and phylogenetic relationships. The two newly sequenced mitogenomes showed high structural conservation and a strong AT bias, consistent with typical features in Siphonaptera. Natural selection was identified as the dominant force driving codon usage bias. Phylogenetic reconstructions revealed that clustered strongly with , while formed a well-supported sister clade, indicating genetic divergence among these subspecies. The observed genetic differentiation is likely driven by long-term geographic isolation and host adaptation. This study establishes an integrative morphological and mitogenomic framework, enriches flea mitochondrial genomic resources, and provides key molecular evidence for the phylogeny, taxonomy, and surveillance of flea vectors.
如何引用
AMA
Mingna Duan, Yuran Xia, Dandan Jiang, Shaobo Tang, Rui Hou, Lanling Tian, et al. {"_":"Mitochondrial genomes of two subspecies of : Insights into the molecular evolution and phylogenetic analysis within Siphonaptera.","i":["Citellophilus tesquorum"]}. Frontiers in veterinary science. 2026; doi:10.3389/fvets.2026.1785674.
APA
Mingna Duan, Yuran Xia, Dandan Jiang, Shaobo Tang, Rui Hou, Lanling Tian, et al (2026). {"_":"Mitochondrial genomes of two subspecies of : Insights into the molecular evolution and phylogenetic analysis within Siphonaptera.","i":["Citellophilus tesquorum"]}. Frontiers in veterinary science. https://doi.org/10.3389/fvets.2026.1785674
GB/T 7714
Mingna Duan, Yuran Xia, Dandan Jiang, Shaobo Tang, Rui Hou, Lanling Tian, et al. {"_":"Mitochondrial genomes of two subspecies of : Insights into the molecular evolution and phylogenetic analysis within Siphonaptera.","i":["Citellophilus tesquorum"]}[J]. Frontiers in veterinary science, 2026 doi:10.3389/fvets.2026.1785674.
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